Duplication of an approximately 1.5 Mb DNA segment
... locus were retained in all informative cases without any sign of allelic duplication. One of the VHL patients with multiple bilateral tumours showed constitutional heterozygosity at APC and MCC loci. Six out of nine tumours showed duplication of one allele of the MCC gene (Table 1b, and Figure 1a), ...
... locus were retained in all informative cases without any sign of allelic duplication. One of the VHL patients with multiple bilateral tumours showed constitutional heterozygosity at APC and MCC loci. Six out of nine tumours showed duplication of one allele of the MCC gene (Table 1b, and Figure 1a), ...
Intrastrand Self-complementary Sequences in Bacillus subtilis DNA
... widely scattered throughout the B. subtilis genome were transformed by the DNA which contained secondary structure suggesting that the location of such sequences is not unique. The biological nature of the self-annealing DNA has been further characterized using transformation assays and by hybridiza ...
... widely scattered throughout the B. subtilis genome were transformed by the DNA which contained secondary structure suggesting that the location of such sequences is not unique. The biological nature of the self-annealing DNA has been further characterized using transformation assays and by hybridiza ...
ERF/AP2 Subfamily A3 and ER/AP2 Subfamily A6 Genes
... Chalazal seed coat/ linear cotyledon stage Chalazal seed coat/ Mature green stage ...
... Chalazal seed coat/ linear cotyledon stage Chalazal seed coat/ Mature green stage ...
A new ferrochelatase mutation combined with low
... [2]. While most EPP patients are associated with skin photosensitivity only, some (5 % to 10 %) develop hepatic failure due to massive hepatic accumulation of protoporphyrin [3]. Since the sequence of ferrochelatase cDNA was published by Nakahashi et al. [4], more than 60 different kinds of molecula ...
... [2]. While most EPP patients are associated with skin photosensitivity only, some (5 % to 10 %) develop hepatic failure due to massive hepatic accumulation of protoporphyrin [3]. Since the sequence of ferrochelatase cDNA was published by Nakahashi et al. [4], more than 60 different kinds of molecula ...
Inheritance of Organelle DNA Sequences in a Citrus–Poncirus
... configurations. The Yates-corrected chisquared test ( Yates 1934) for a 1:1 segregation in this population was 1.9 (P ⬎ .1). This hypothesis cannot be confirmed directly because the paternal tree is no longer living. Given that P. trifoliata trees are naturally outcrossing and highly heterozygous ( ...
... configurations. The Yates-corrected chisquared test ( Yates 1934) for a 1:1 segregation in this population was 1.9 (P ⬎ .1). This hypothesis cannot be confirmed directly because the paternal tree is no longer living. Given that P. trifoliata trees are naturally outcrossing and highly heterozygous ( ...
slides
... - runs of three or more Cs or Gs at the 3'-ends of primers may promote mispriming at G or C-rich sequences (because of stability of annealing), and should be avoided; - 3'-ends of primers should not be complementary (ie. base pair), as otherwise primer dimers will be synthesised preferentially to an ...
... - runs of three or more Cs or Gs at the 3'-ends of primers may promote mispriming at G or C-rich sequences (because of stability of annealing), and should be avoided; - 3'-ends of primers should not be complementary (ie. base pair), as otherwise primer dimers will be synthesised preferentially to an ...
DNA Mismatch Repair and Synonymous Codon Evolution in
... Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855 1059. Mol. Biol. Evol. 11(1):88-98. 1994. ...
... Department of Biological Sciences, Rutgers University, Piscataway, New Jersey 08855 1059. Mol. Biol. Evol. 11(1):88-98. 1994. ...
DNA - Armstrong State University
... For the forensic scientist, PCR offers a distinct advantage in that it can amplify minute quantities of DNA many millions of times. First, the DNA is heated to separate it. Second, primers (short strands of DNA used to target specific regions of DNA for replication) are added which hybridize with th ...
... For the forensic scientist, PCR offers a distinct advantage in that it can amplify minute quantities of DNA many millions of times. First, the DNA is heated to separate it. Second, primers (short strands of DNA used to target specific regions of DNA for replication) are added which hybridize with th ...
MOLLECULAR BIOLOGY COURSE
... (A), guanine (G), cytosine (C), and thymine (T). Human DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the information available for building and maintaining an organism, similar to the way ...
... (A), guanine (G), cytosine (C), and thymine (T). Human DNA consists of about 3 billion bases, and more than 99 percent of those bases are the same in all people. The order, or sequence, of these bases determines the information available for building and maintaining an organism, similar to the way ...
Reaction of Systemic Lupus Erythematosus Antinative DNA
... Most SLE patients are ill when their sera contain antinative DNA, but some produce DNA-binding antibodies at times of little disease activity (8, 9). Factors such as complement-fixing activity (9-12) or functional affinity (13, 14) may be important in determining the variable pathogenetic potential ...
... Most SLE patients are ill when their sera contain antinative DNA, but some produce DNA-binding antibodies at times of little disease activity (8, 9). Factors such as complement-fixing activity (9-12) or functional affinity (13, 14) may be important in determining the variable pathogenetic potential ...
DNA Methylation Mechanisms and Analysis Methods to
... • Primers do not contain any CpG sites, but do contain non-CpG cytosines • Bisulfite-specific primer pair: amplifies both bisulfite-converted methylated and bisulfite converted unmethylated DNA • Wildtype-specific primer pair: amplifies unconverted DNA, both methylated or unmethylated sequences ...
... • Primers do not contain any CpG sites, but do contain non-CpG cytosines • Bisulfite-specific primer pair: amplifies both bisulfite-converted methylated and bisulfite converted unmethylated DNA • Wildtype-specific primer pair: amplifies unconverted DNA, both methylated or unmethylated sequences ...
Selection of Functional Signal Peptide Cleavage Sites from a Library of Random Sequences.
... equilibrated with the same buffer as that used for dialysis (3). Fractions containing ,B-lactamase activity were identified by nitrocefin hydrolysis and SDS-PAGE (16). For amino-terminal sequencing, protein from fractions with the highest purity (80 to 90% pure) was fractionated by SDS-PAGE and elec ...
... equilibrated with the same buffer as that used for dialysis (3). Fractions containing ,B-lactamase activity were identified by nitrocefin hydrolysis and SDS-PAGE (16). For amino-terminal sequencing, protein from fractions with the highest purity (80 to 90% pure) was fractionated by SDS-PAGE and elec ...
NUCLEIC ACIDS
... divided and packaged into 46 separate structures known as chromosomes. During the interphase, when the DNA needs to be accessible to the transcription and replication enzymes, it is packaged into less densely mitotic chromosomes known as chromatin. Chromatin can be detected by microscopic staining, ...
... divided and packaged into 46 separate structures known as chromosomes. During the interphase, when the DNA needs to be accessible to the transcription and replication enzymes, it is packaged into less densely mitotic chromosomes known as chromatin. Chromatin can be detected by microscopic staining, ...
Leading strand
... nucleotides are added. – DNA polymerase III extends the strand in the 5’-to-3’ direction – DNA polymerase I degrades the RNA primer and replaces it with DNA – DNA ligase joins the DNA fragments into a continuous daughter strand Overall direction of replication ...
... nucleotides are added. – DNA polymerase III extends the strand in the 5’-to-3’ direction – DNA polymerase I degrades the RNA primer and replaces it with DNA – DNA ligase joins the DNA fragments into a continuous daughter strand Overall direction of replication ...
shERWOOD-UltramiR shRNA
... Knockdown efficiencies of shERWOOD-UltramiR hairpins were benchmarked against existing TRC and GIPZ early generation shRNA-mir hairpins targeting 3 different genes. shERWOOD-UltramiR designs produced very potent and consistent knockdown relative to available TRC and GIPZ hairpins targeting the same ...
... Knockdown efficiencies of shERWOOD-UltramiR hairpins were benchmarked against existing TRC and GIPZ early generation shRNA-mir hairpins targeting 3 different genes. shERWOOD-UltramiR designs produced very potent and consistent knockdown relative to available TRC and GIPZ hairpins targeting the same ...
Gene Section FANCD2 (Fanconi anemia, complementation group D2) Atlas of Genetics and Cytogenetics
... FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This FA complex translocates into the nucleus, where FANCE and FANCF are present; FANCE and FANCF join the complex. The FA complex subsequently interacts with FANCD2 by monoubiquitination of FANCD2 durin ...
... FANCC join the complex; phosphorylation of FANCA would induce its translocation into the nucleus.This FA complex translocates into the nucleus, where FANCE and FANCF are present; FANCE and FANCF join the complex. The FA complex subsequently interacts with FANCD2 by monoubiquitination of FANCD2 durin ...
Transformation of Clostridium Thermocellum by Electroporation
... orientation of the 50 –30 flanks and the int region affects the resulting intermediate chromosomal configuration. If the orientation of the flanks is the same as that shown in Fig. 17.3 (i.e., the order is 50 flank, 30 flank, cat, hpt, and int region), the intermediate chromosomal configuration will ...
... orientation of the 50 –30 flanks and the int region affects the resulting intermediate chromosomal configuration. If the orientation of the flanks is the same as that shown in Fig. 17.3 (i.e., the order is 50 flank, 30 flank, cat, hpt, and int region), the intermediate chromosomal configuration will ...
Text S2.
... DivL expression vectors Primers and plasmids used in this study are shown in Supplementary Tables S2-S3. A fragment of divL encoding residues 523-769 (used in crystallization) was amplified by PCR from the template cosmid 2G1 [1] using primers WSCp8 and WSCp11. These primers introduced NdeI and EcoR ...
... DivL expression vectors Primers and plasmids used in this study are shown in Supplementary Tables S2-S3. A fragment of divL encoding residues 523-769 (used in crystallization) was amplified by PCR from the template cosmid 2G1 [1] using primers WSCp8 and WSCp11. These primers introduced NdeI and EcoR ...
6 Core Chapter 6
... Real-World Case Study: In the 1990s a private company discovered a set of genes that increase the risk of getting breast and ovarian cancers (BRCA1 and BRCA2). They applied for and received a patent for the genes, which means they are the only company that can offer a genetic test for the BRCA1 and ...
... Real-World Case Study: In the 1990s a private company discovered a set of genes that increase the risk of getting breast and ovarian cancers (BRCA1 and BRCA2). They applied for and received a patent for the genes, which means they are the only company that can offer a genetic test for the BRCA1 and ...
2.5.1 Variation of Species 2.5.2 Heredity and Gene
... 2.5.4 DNA Structure, Replication and Profiling Follow-Me – iQuiz ...
... 2.5.4 DNA Structure, Replication and Profiling Follow-Me – iQuiz ...
replication v 2015_21
... DNA Polymerase – enzyme that catalyzes connection of nucleotides to form complementary DNA strand in 5’ to 3’ direction (reads template in 3’ to 5’ direction) Leading Strand – transcribed continuously in 5’ to 3’ direction Lagging Strand – transcribed in segments in 5’ to 3’ direction (Okazaki fragm ...
... DNA Polymerase – enzyme that catalyzes connection of nucleotides to form complementary DNA strand in 5’ to 3’ direction (reads template in 3’ to 5’ direction) Leading Strand – transcribed continuously in 5’ to 3’ direction Lagging Strand – transcribed in segments in 5’ to 3’ direction (Okazaki fragm ...
The Functional Organization of the Vestigial Locus in Drosophila
... DNA sequencing of the putative 3′ end of cDNA1 identified two overlapping poly(A) addition sites preceded by a third poly(A) site. DNA sequencing of M13 clones of genomic vg DNA from the +16 SalI site indicates that these poly(A) addition sites are located approximately 150 bp proximal to the SalI s ...
... DNA sequencing of the putative 3′ end of cDNA1 identified two overlapping poly(A) addition sites preceded by a third poly(A) site. DNA sequencing of M13 clones of genomic vg DNA from the +16 SalI site indicates that these poly(A) addition sites are located approximately 150 bp proximal to the SalI s ...
Justification of Size Estimates for Tomato Genome Sequencing
... flanking heterochromatin sequences (Wu et al., 2004, Yan et al., 2005). The tomato genome is comprised of a majority of paracentric heterochromatin typically flanked by large euchromatin islands that comprise the majority of the chromosome “arms” (see below). For the purpose of the international tom ...
... flanking heterochromatin sequences (Wu et al., 2004, Yan et al., 2005). The tomato genome is comprised of a majority of paracentric heterochromatin typically flanked by large euchromatin islands that comprise the majority of the chromosome “arms” (see below). For the purpose of the international tom ...
Restriction Enzyme digestion of DNA
... • A special class of endonucleases from a bacteria has been isolated for this experiment. These special enzymes, termed restriction endonucleases (RE), digest DNA by breaking bonds only within a specific short sequence of bases. These base sequences usually ran in size from 48 base pairs but can be ...
... • A special class of endonucleases from a bacteria has been isolated for this experiment. These special enzymes, termed restriction endonucleases (RE), digest DNA by breaking bonds only within a specific short sequence of bases. These base sequences usually ran in size from 48 base pairs but can be ...
Genotyping of urinary samples stored with EDTA for
... Baseline assessment of the mass concentration of fresh urinary DNA showed that Chinese men had significantly less urinary DNA than Chinese women did. A similar decrease in the amount of human urinary DNA in men has been found in studies undertaken in the United States (Johnson et al., 2007), Italy ( ...
... Baseline assessment of the mass concentration of fresh urinary DNA showed that Chinese men had significantly less urinary DNA than Chinese women did. A similar decrease in the amount of human urinary DNA in men has been found in studies undertaken in the United States (Johnson et al., 2007), Italy ( ...