DNA Technology Notes
... organism into a different organism. This changing of an organism’s DNA to give the organism new traits is called genetic engineering. It is based on the use of recombinant DNA technology. Recombinant DNA is DNA that contains genes from more than one organism. First GMO was in 1973– bacteria. Bacteri ...
... organism into a different organism. This changing of an organism’s DNA to give the organism new traits is called genetic engineering. It is based on the use of recombinant DNA technology. Recombinant DNA is DNA that contains genes from more than one organism. First GMO was in 1973– bacteria. Bacteri ...
Lab 8
... Most cells in our body contain DNA, the molecule that makes up the genes that control our physical characteristics. Half your DNA came from your mother, and half from your father. Your DNA is what determines your eye color, hair color, height, blood type, and all other physical properties of your bo ...
... Most cells in our body contain DNA, the molecule that makes up the genes that control our physical characteristics. Half your DNA came from your mother, and half from your father. Your DNA is what determines your eye color, hair color, height, blood type, and all other physical properties of your bo ...
short_answer_Barcoding_exam_Key
... Spin column—silca matrix is negatively charged, but Na+ ions create a cation bridge, so DNA sticks to matrix, then we wash away the cation bridge with water and the DNA is liberated 36. What happens to the DNA template when put in the spin column? (2) The DNA template also sticks to the silica matr ...
... Spin column—silca matrix is negatively charged, but Na+ ions create a cation bridge, so DNA sticks to matrix, then we wash away the cation bridge with water and the DNA is liberated 36. What happens to the DNA template when put in the spin column? (2) The DNA template also sticks to the silica matr ...
DNA Quantification
... equivalent to 50 µg DNA per ml (standard). We are taking 50 µl of DNA in 1 ml TE buffer, which means it is diluted 20 times. For example: if the spectrophotometer reading is 0.112 (say), then the concentration of DNA is calculated as, DNA concentration (µg/µl) = 0.112 × 20 × 50= 112 µg/ml most DNA e ...
... equivalent to 50 µg DNA per ml (standard). We are taking 50 µl of DNA in 1 ml TE buffer, which means it is diluted 20 times. For example: if the spectrophotometer reading is 0.112 (say), then the concentration of DNA is calculated as, DNA concentration (µg/µl) = 0.112 × 20 × 50= 112 µg/ml most DNA e ...
Gene Cloning Technology
... Restriction enzymes cut DNA infrequently The frequency with which this particular sequence of 6 base pairs will occur in a very large DNA molecule can be calculated on the basis of: the probability of occurrence of any specified base at each position: ...
... Restriction enzymes cut DNA infrequently The frequency with which this particular sequence of 6 base pairs will occur in a very large DNA molecule can be calculated on the basis of: the probability of occurrence of any specified base at each position: ...
The Structure of DNA
... Each experiment includes special safety precautions that are relevant to that particular project. These do not include all the basic safety precautions that are necessary whenever you are working on a scientific experiment. For this reason, it is absolutely necessary that you read and remain mindful ...
... Each experiment includes special safety precautions that are relevant to that particular project. These do not include all the basic safety precautions that are necessary whenever you are working on a scientific experiment. For this reason, it is absolutely necessary that you read and remain mindful ...
Gene Cloning Technology
... Restriction enzymes cut DNA infrequently The frequency with which this particular sequence of 6 base pairs will occur in a very large DNA molecule can be calculated on the basis of: the probability of occurrence of any specified base at each position: ...
... Restriction enzymes cut DNA infrequently The frequency with which this particular sequence of 6 base pairs will occur in a very large DNA molecule can be calculated on the basis of: the probability of occurrence of any specified base at each position: ...
Gene sequencing Terms
... • The term "wild type" allele is sometimes used to describe an allele that is thought to contribute to the typical phenotypic character as seen in "wild" populations of organisms. • Such a "wild type" allele was historically regarded as dominant, common, and "normal", in contrast to "mutant" alleles ...
... • The term "wild type" allele is sometimes used to describe an allele that is thought to contribute to the typical phenotypic character as seen in "wild" populations of organisms. • Such a "wild type" allele was historically regarded as dominant, common, and "normal", in contrast to "mutant" alleles ...
NUCLEIC ACID STRUCTURE AND DNA REPLICATION
... primase makes one RNA primer DNA polymerase attaches nucleotides in a 5’ to 3’ direction as it slides forward ...
... primase makes one RNA primer DNA polymerase attaches nucleotides in a 5’ to 3’ direction as it slides forward ...
T4 DNA Polymerase
... Extensive labeling is achieved by the replacement reaction, in which the 3´-exonuclease activity of the enzyme first digests dsDNA to produce molecules with recessed 3´-termini (7). On subsequent addition of labeled dNTPs, the polymerase activity of T4 DNA polymerase then extends the 3´-ends along t ...
... Extensive labeling is achieved by the replacement reaction, in which the 3´-exonuclease activity of the enzyme first digests dsDNA to produce molecules with recessed 3´-termini (7). On subsequent addition of labeled dNTPs, the polymerase activity of T4 DNA polymerase then extends the 3´-ends along t ...
Practical 1
... 5. Repeate step 1 and 2 for protein sequence by generating an amino acid polypeptide of length 100 and retriving the most over-‐represented amino acid in the sequence. 6. From the DNA sequence generated ...
... 5. Repeate step 1 and 2 for protein sequence by generating an amino acid polypeptide of length 100 and retriving the most over-‐represented amino acid in the sequence. 6. From the DNA sequence generated ...
Nucleic Acid Biotechnology Techniques
... • DNA can be sequenced by using several techniques, the most common being the chain termination method • Dideoxy nucleotides are used to terminate DNA synthesis. Multiple reactions are run with different dideoxy nucleotide in each reaction mix • The reactions produce a series of DNA fragments of dif ...
... • DNA can be sequenced by using several techniques, the most common being the chain termination method • Dideoxy nucleotides are used to terminate DNA synthesis. Multiple reactions are run with different dideoxy nucleotide in each reaction mix • The reactions produce a series of DNA fragments of dif ...
Nucleic Acid Test A
... 33__________The new strand of DNA which is laid down in a direction opposite of the movement of helicase, resulting in fragments of DNA which later need to be connected by ligase is known as the A) leading strand. B) lagging strand. C) semiconservative strand. D) antiparallel strand. E) degenerate s ...
... 33__________The new strand of DNA which is laid down in a direction opposite of the movement of helicase, resulting in fragments of DNA which later need to be connected by ligase is known as the A) leading strand. B) lagging strand. C) semiconservative strand. D) antiparallel strand. E) degenerate s ...
DNA
... – When a gene coding for a human protein (like a hormone or enzyme) is inserted into bacteria, the new recombinant cells may produce LARGE amounts of the protein. – The human growth hormone, a hormone required for growth and development, was incredibly rare before genetic engineering. – Now these tr ...
... – When a gene coding for a human protein (like a hormone or enzyme) is inserted into bacteria, the new recombinant cells may produce LARGE amounts of the protein. – The human growth hormone, a hormone required for growth and development, was incredibly rare before genetic engineering. – Now these tr ...
8-DNA
... A. showed that different species possess DNA with different ratios of nitrogenous bases: _____________________________. B. showed that bacteria can acquire new genetic information from their surroundings: _______________________; and who later showed that that genetic information appeared to be nucl ...
... A. showed that different species possess DNA with different ratios of nitrogenous bases: _____________________________. B. showed that bacteria can acquire new genetic information from their surroundings: _______________________; and who later showed that that genetic information appeared to be nucl ...
DNA - Biology
... Procedures used to isolate DNA and Determine VNTRs The basic procedure used to isolate an individual's DNA fingerprint is called Restriction Fragment Length Polymorphism (RFLP) analysis. In RFLP analysis, restriction enzymes (restriction endonucleases) cut the surrounding regions of VNTRs at certain ...
... Procedures used to isolate DNA and Determine VNTRs The basic procedure used to isolate an individual's DNA fingerprint is called Restriction Fragment Length Polymorphism (RFLP) analysis. In RFLP analysis, restriction enzymes (restriction endonucleases) cut the surrounding regions of VNTRs at certain ...
Strawberry DNA Extraction Lab (This promises to be berry interesting!)
... Background: DNA is found in the cells of every living organism. DNA is a double stranded molecule composed of nucleotide bases pairing Adenine with Thymine and Guanine with Cytosine. Strawberries are soft and easy to pulverize. Strawberries are polyploidy, meaning they have large genomes with many s ...
... Background: DNA is found in the cells of every living organism. DNA is a double stranded molecule composed of nucleotide bases pairing Adenine with Thymine and Guanine with Cytosine. Strawberries are soft and easy to pulverize. Strawberries are polyploidy, meaning they have large genomes with many s ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.