High-Throughput Analysis of Foodborne Bacterial Genomic DNA
... will contribute to UV-absorbance at 260 nm. However, for DNA fragmentation and library construction steps, input of specific quantities of HMW double-stranded DNA are required. Fluorescence-based methods using an intercalated dye are commonly used to quantify dsDNA. One of the methods, Qubit, is the ...
... will contribute to UV-absorbance at 260 nm. However, for DNA fragmentation and library construction steps, input of specific quantities of HMW double-stranded DNA are required. Fluorescence-based methods using an intercalated dye are commonly used to quantify dsDNA. One of the methods, Qubit, is the ...
Supplementary Text 1 (doc 52K)
... sequences not included in the MMC showed no mismatch. As the first primer set covers more sequences of the MMC than the second, the first system was chosen for screening of environmental samples. To determine the optimal annaeling temperatures for PCR and to avoid unspecific amplification, DNA from ...
... sequences not included in the MMC showed no mismatch. As the first primer set covers more sequences of the MMC than the second, the first system was chosen for screening of environmental samples. To determine the optimal annaeling temperatures for PCR and to avoid unspecific amplification, DNA from ...
Molecular Biology I
... There was some 35S label found associated with the bacterial cells – so some people believed that the genetic material laws the protein that was stuck to the cell BUT, in general, most people believed the DNA was the genetic material These experiments reinforced the notions proposed earlier by Avery ...
... There was some 35S label found associated with the bacterial cells – so some people believed that the genetic material laws the protein that was stuck to the cell BUT, in general, most people believed the DNA was the genetic material These experiments reinforced the notions proposed earlier by Avery ...
Pfu DNA Polymerase Product Information 9PIM774
... of enzyme to amplify a 1,200bp region of the α-1-antitrypsin gene from 100 molecules (0.33ng) of human genomic DNA. The resulting PCR product is visualized on an ethidium bromide-stained agarose gel. Standard DNA Polymerase Assay Conditions (not PCR conditions): The polymerase activity is assayed in ...
... of enzyme to amplify a 1,200bp region of the α-1-antitrypsin gene from 100 molecules (0.33ng) of human genomic DNA. The resulting PCR product is visualized on an ethidium bromide-stained agarose gel. Standard DNA Polymerase Assay Conditions (not PCR conditions): The polymerase activity is assayed in ...
Document
... DNA profiling is the use of molecular genetic methods to determine the exact genotype of a DNA sample in a way that can basically distinguish one human being from another The unique genotype of each sample is called a DNA profile. ...
... DNA profiling is the use of molecular genetic methods to determine the exact genotype of a DNA sample in a way that can basically distinguish one human being from another The unique genotype of each sample is called a DNA profile. ...
Bio Rad PCR Song Lyrics
... 6. How many molecules of double-stranded DNA will you have after three cycles? After five cycles? After 30 cycles? 3 cycles = 8 ds DNA (23); 5 cycles = 32 ds DNA (25); 30 cycles = 1,073,741,824 (230) ...
... 6. How many molecules of double-stranded DNA will you have after three cycles? After five cycles? After 30 cycles? 3 cycles = 8 ds DNA (23); 5 cycles = 32 ds DNA (25); 30 cycles = 1,073,741,824 (230) ...
Student Genetic recombination
... will carry a different recombinant DNA (that is, a different cloned insert). Therefore, the next step is to find a way to select the clone with the insert containing the specific gene in which we are interested. When this clone has been obtained, the DNA is isolated in bulk and the cloned gene of in ...
... will carry a different recombinant DNA (that is, a different cloned insert). Therefore, the next step is to find a way to select the clone with the insert containing the specific gene in which we are interested. When this clone has been obtained, the DNA is isolated in bulk and the cloned gene of in ...
Determination of the DNA and Amino Acid Sequences of the Lactate
... in media for the selection and growth of transformants. T4 DNA Ligase and restriction enzymes (Eco RI and Pst I) were purchased from Boehringer Corp. Taq DNA Polymerase was obtained from Pharmacia Biotech, Uppsala, Sweden. Polymerase Chain Reaction (PCR) Two oligonucleotide primers used to amplify P ...
... in media for the selection and growth of transformants. T4 DNA Ligase and restriction enzymes (Eco RI and Pst I) were purchased from Boehringer Corp. Taq DNA Polymerase was obtained from Pharmacia Biotech, Uppsala, Sweden. Polymerase Chain Reaction (PCR) Two oligonucleotide primers used to amplify P ...
November 2010 Prof Angela van Daal Forensic DNA
... Flanking regions are the stretches of DNA outside the region of interest. For STRs for example, these sequences are the non-repeated DNA regions which, unlike the repeat regions, are are the same amongst individuals. The primer sequences are designed from DNA in the flanking regions such that they w ...
... Flanking regions are the stretches of DNA outside the region of interest. For STRs for example, these sequences are the non-repeated DNA regions which, unlike the repeat regions, are are the same amongst individuals. The primer sequences are designed from DNA in the flanking regions such that they w ...
Comprehension Questions Key
... A DNA barcode is a metaphor for UPC barcodes that are used to identify and track retail products. Like UPC barcodes that are used to uniquely identify products, DNA sequences can be used to uniquely identify species. Each position is encoded by a nucleotide, this results in 4 possible nucleotides at ...
... A DNA barcode is a metaphor for UPC barcodes that are used to identify and track retail products. Like UPC barcodes that are used to uniquely identify products, DNA sequences can be used to uniquely identify species. Each position is encoded by a nucleotide, this results in 4 possible nucleotides at ...
Interactive Computer Program: Packaging DNA into Chromosomes
... of proteins, into thread-like structures called chromosomes. In prokaryotes (such as bacteria), the chromosomal DNA, when open, is often circular. The total length of a bacterial chromosomal DNA (e.g., E. coli DNA) may be a thousand times longer than the cell that contains it. Little is known about ...
... of proteins, into thread-like structures called chromosomes. In prokaryotes (such as bacteria), the chromosomal DNA, when open, is often circular. The total length of a bacterial chromosomal DNA (e.g., E. coli DNA) may be a thousand times longer than the cell that contains it. Little is known about ...
in Power-Point Format
... • Double helical model for DNA: complementary strands • Each strand is template for new partner strand – Semiconservative model for DNA replication 5’ -> 3’ – Leading strand continuous synthesis – Half-discontinuous (short pieces on lagging strand are later stitched together) – Requires RNA primers ...
... • Double helical model for DNA: complementary strands • Each strand is template for new partner strand – Semiconservative model for DNA replication 5’ -> 3’ – Leading strand continuous synthesis – Half-discontinuous (short pieces on lagging strand are later stitched together) – Requires RNA primers ...
Chapt 20 DNA Replication I: Basic Mechanism and Enyzmology
... • Double helical model for DNA: complementary strands • Each strand is template for new partner strand – Semiconservative model for DNA replication 5’ -> 3’ – Leading strand continuous synthesis – Half-discontinuous (short pieces on lagging strand are later stitched together) – Requires RNA primers ...
... • Double helical model for DNA: complementary strands • Each strand is template for new partner strand – Semiconservative model for DNA replication 5’ -> 3’ – Leading strand continuous synthesis – Half-discontinuous (short pieces on lagging strand are later stitched together) – Requires RNA primers ...
Data Analysis for Next Generation Sequencing: An
... Interpretation or tertiary data analysis, is the most complex, experiment-specific, time-consuming and manual phase of the NGS data analysis pipeline. To put things in perspective, a germline whole exome sequencing (WES) generates an average of about 20,000 SNPs (3) or even 30,000 in the case of Agi ...
... Interpretation or tertiary data analysis, is the most complex, experiment-specific, time-consuming and manual phase of the NGS data analysis pipeline. To put things in perspective, a germline whole exome sequencing (WES) generates an average of about 20,000 SNPs (3) or even 30,000 in the case of Agi ...
DNA Replication
... – They therefore require multiple origins of replication • To ensure that the DNA can be replicated in a reasonable time ...
... – They therefore require multiple origins of replication • To ensure that the DNA can be replicated in a reasonable time ...
Phasing Analysis Service for Whole Human Genome Sequencing
... Enabling Comprehensive Analysis of Complex Traits Because genome phasing technology provides haplotype information about each individual genome, it enables allele-specific and variant linkage analysis (Figure 3). This knowledge can inform studies of complex trait susceptibility, as allelic interacti ...
... Enabling Comprehensive Analysis of Complex Traits Because genome phasing technology provides haplotype information about each individual genome, it enables allele-specific and variant linkage analysis (Figure 3). This knowledge can inform studies of complex trait susceptibility, as allelic interacti ...
Biology 115 Lab 10:Gene Technology
... DNA DNA is the hereditary molecule. The information necessary to build an organism is encoded in the bases of a DNA molecule. Deoxyribonucleic Acid (DNA) is composed of subunits called nucleotides. A single nucleotide has three parts: a phosphate group, a 5′-carbon sugar (deoxyribose) and a nitrogen ...
... DNA DNA is the hereditary molecule. The information necessary to build an organism is encoded in the bases of a DNA molecule. Deoxyribonucleic Acid (DNA) is composed of subunits called nucleotides. A single nucleotide has three parts: a phosphate group, a 5′-carbon sugar (deoxyribose) and a nitrogen ...
Bulletin - Sigma
... AccuTaq LA Polymerase Mix combines Sigma’s high quality Taq DNA polymerase with a small amount of a thermostable proofreading enzyme. The result is an enzyme mix that amplifies genomic targets in excess of 20 kb. Using a less complex template, such as bacterial genomic and viral DNA, amplifications ...
... AccuTaq LA Polymerase Mix combines Sigma’s high quality Taq DNA polymerase with a small amount of a thermostable proofreading enzyme. The result is an enzyme mix that amplifies genomic targets in excess of 20 kb. Using a less complex template, such as bacterial genomic and viral DNA, amplifications ...
Suppl. Material
... product was then digested with BglII (Figure 2A) which has 3 cutting sites resulting in four different sizes (30bp, 162bp, 306bp and 35bp). The larger sized fragment of 306bp was cut from the gel using Qiagen gel extraction kit and purified. The 571bp waaG PCR product was also digested with BglII ( ...
... product was then digested with BglII (Figure 2A) which has 3 cutting sites resulting in four different sizes (30bp, 162bp, 306bp and 35bp). The larger sized fragment of 306bp was cut from the gel using Qiagen gel extraction kit and purified. The 571bp waaG PCR product was also digested with BglII ( ...
File
... template to direct the synthesis of other strand similar to its complementary one. • Thus nucleic acids are uniquely capable of directing their own self replication. • The diameter of the helix could only be kept constant at about 2 nm or 20 Å if one purine and one pyrimidine base made up each stair ...
... template to direct the synthesis of other strand similar to its complementary one. • Thus nucleic acids are uniquely capable of directing their own self replication. • The diameter of the helix could only be kept constant at about 2 nm or 20 Å if one purine and one pyrimidine base made up each stair ...
Chapter 16
... 5. The backbone of each single DNA chain is formed by alternating deoxyribose and phosphate groups joined by phosphodiester linkages. 6. Each phosphate group is linked to the 5’ carbon of one deoxyribose and to the 3’ carbon of the other deoxyribose. 7. Hydrogen bonds form between adenine and thymin ...
... 5. The backbone of each single DNA chain is formed by alternating deoxyribose and phosphate groups joined by phosphodiester linkages. 6. Each phosphate group is linked to the 5’ carbon of one deoxyribose and to the 3’ carbon of the other deoxyribose. 7. Hydrogen bonds form between adenine and thymin ...
DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery.Knowledge of DNA sequences has become indispensable for basic biological research, and in numerous applied fields such as medical diagnosis, biotechnology, forensic biology, virology and biological systematics. The rapid speed of sequencing attained with modern DNA sequencing technology has been instrumental in the sequencing of complete DNA sequences, or genomes of numerous types and species of life, including the human genome and other complete DNA sequences of many animal, plant, and microbial species.The first DNA sequences were obtained in the early 1970s by academic researchers using laborious methods based on two-dimensional chromatography. Following the development of fluorescence-based sequencing methods with a DNA sequencer, DNA sequencing has become easier and orders of magnitude faster.