BIOLOGY-DNA and Protein Synthesis PPT
... 1952 Hershey and Chase Experiment: - used bacteriophages - a virus that attacks bacteria Question: Is the Protein Coat or DNA or both enter the bacteria to kill it? -used one type of radioactive marker to mark the protein coat -used another type radioactive marker to mark the DNA Results- nearly al ...
... 1952 Hershey and Chase Experiment: - used bacteriophages - a virus that attacks bacteria Question: Is the Protein Coat or DNA or both enter the bacteria to kill it? -used one type of radioactive marker to mark the protein coat -used another type radioactive marker to mark the DNA Results- nearly al ...
BIOTECHNOLOGY
... occurring enzymes that act like a pair of molecular scissors to cut DNA in a predictable and precise manner, at a specific nucleotide sequence called a recognition site . Hamilton Smith, John Hopkins University, won the Nobel Prize in 1978 for discovering restriction enzymes in bacteria (Hind III). ...
... occurring enzymes that act like a pair of molecular scissors to cut DNA in a predictable and precise manner, at a specific nucleotide sequence called a recognition site . Hamilton Smith, John Hopkins University, won the Nobel Prize in 1978 for discovering restriction enzymes in bacteria (Hind III). ...
Gene Cloning Technology
... 2. DNA Cloning Vectors A cloning vector is a DNA molecule that can contains information for replicating itself – a so-called “origin of replication” If a piece of foreign DNA is incorporated into the vector, then the foreign DNA will be replicated along with the vector DNA The most commonl ...
... 2. DNA Cloning Vectors A cloning vector is a DNA molecule that can contains information for replicating itself – a so-called “origin of replication” If a piece of foreign DNA is incorporated into the vector, then the foreign DNA will be replicated along with the vector DNA The most commonl ...
Nucleic acids
... The middle hydrogen bonds run in opposite directions. In the middle bond, T donates a hydrogen to an acceptor on A. C does not have a hydrogen to donate in the middle position; rather, it accepts a hydrogen from G's middle position. The same is true of the "third hydrogen bond" (the one that is abse ...
... The middle hydrogen bonds run in opposite directions. In the middle bond, T donates a hydrogen to an acceptor on A. C does not have a hydrogen to donate in the middle position; rather, it accepts a hydrogen from G's middle position. The same is true of the "third hydrogen bond" (the one that is abse ...
DNA Replication
... B) The leading strand is synthesized 5’3’, but the lagging strand is synthesized ...
... B) The leading strand is synthesized 5’3’, but the lagging strand is synthesized ...
Gene Cloning Technology
... 2. DNA Cloning Vectors A cloning vector is a DNA molecule that can contains information for replicating itself – a so-called “origin of replication” If a piece of foreign DNA is incorporated into the vector, then the foreign DNA will be replicated along with the vector DNA The most commonl ...
... 2. DNA Cloning Vectors A cloning vector is a DNA molecule that can contains information for replicating itself – a so-called “origin of replication” If a piece of foreign DNA is incorporated into the vector, then the foreign DNA will be replicated along with the vector DNA The most commonl ...
DNA Spooling vB - College of the Canyons
... DNA Spooling…it all starts here with the isolation and purification DNA from the other molecules in a cell. While it can be extracted from almost any living or preserved tissue, we will use bananas or another fruit, as they are easy to collect. In this lab you will isolate DNA using common household ...
... DNA Spooling…it all starts here with the isolation and purification DNA from the other molecules in a cell. While it can be extracted from almost any living or preserved tissue, we will use bananas or another fruit, as they are easy to collect. In this lab you will isolate DNA using common household ...
Chapter 9 DNA: The Genetic Material
... - Base Pairing Rules state that C always bonds with G A always bonds with T ...
... - Base Pairing Rules state that C always bonds with G A always bonds with T ...
Last Name: First Name: Per. _____ Parent Signature: Pre
... Number of strands _2- double___. Parallel or antiparallel? __antiparallel____. Rosalind Franklin’s x-ray crystallography image indicated that the 3-D shape of the molecule was __helical_. Chargaff’s ratios indicated that there is complementary base pairing: A with _T_ and C with G_. If the amount of ...
... Number of strands _2- double___. Parallel or antiparallel? __antiparallel____. Rosalind Franklin’s x-ray crystallography image indicated that the 3-D shape of the molecule was __helical_. Chargaff’s ratios indicated that there is complementary base pairing: A with _T_ and C with G_. If the amount of ...
Section 8 – The human genome project
... •Since each cut with a known enzyme is a specific base sequence comparing restriction maps allows biologists to look for the numbers and locations of these base sequences. The theory is that the greater the number of sequences and the closer their location on the DNA the more closely related the ind ...
... •Since each cut with a known enzyme is a specific base sequence comparing restriction maps allows biologists to look for the numbers and locations of these base sequences. The theory is that the greater the number of sequences and the closer their location on the DNA the more closely related the ind ...
BIOLOGY I HONORS Course Code - Science - Miami
... trace the history of those cancer cells back to when they were healthy. ...
... trace the history of those cancer cells back to when they were healthy. ...
MUTATIONS
... agents can all cause mutations. Nitrous acid, found in cigarette smoke changes cytosine to uracil. It can also substitute wrong, but similar chemicals for the bases adenine and guanine so when a substituted base DNA tries to replicate all sorts of mistakes are made. ...
... agents can all cause mutations. Nitrous acid, found in cigarette smoke changes cytosine to uracil. It can also substitute wrong, but similar chemicals for the bases adenine and guanine so when a substituted base DNA tries to replicate all sorts of mistakes are made. ...
Analysis of Gene Sequences
... (1) A crude preparation of chromosomal DNA is extracted from the bacterial strain of interest. (2) Two short oligo nucleotide primers (each about 18 bases long) are added to the DNA. The primers are designed from the known genomic sequence to be complimentary to opposite strands of DNA and to flank ...
... (1) A crude preparation of chromosomal DNA is extracted from the bacterial strain of interest. (2) Two short oligo nucleotide primers (each about 18 bases long) are added to the DNA. The primers are designed from the known genomic sequence to be complimentary to opposite strands of DNA and to flank ...
Manipulating DNA - Emerald Meadow Stables
... – Used to separate DNA fragments. DNA fragments placed in a gel and electricity is applied to the gel. DNA molecules are negatively charged and move towards the positive end of the gel. Smaller DNA fragments move faster and farther – This technique used to compare the genomes of different organisms ...
... – Used to separate DNA fragments. DNA fragments placed in a gel and electricity is applied to the gel. DNA molecules are negatively charged and move towards the positive end of the gel. Smaller DNA fragments move faster and farther – This technique used to compare the genomes of different organisms ...
Document
... To start the process, a quartz wafer is coated with a light-sensitive compound (mask). This mask prevents coupling between the wafer and an unwanted nucleotide. The surface is then covered with a solution containing either adenine, or thymine, or cytosine, or guanine. Coupling of the nucleotid to th ...
... To start the process, a quartz wafer is coated with a light-sensitive compound (mask). This mask prevents coupling between the wafer and an unwanted nucleotide. The surface is then covered with a solution containing either adenine, or thymine, or cytosine, or guanine. Coupling of the nucleotid to th ...
Genetic Variation and DNA Markers in Forensic Analysis
... Second, Better protection: The mitochondrion also has a strong protein coat that protects the mitochondrial DNA from degradation by bacterial enzymes. This compares to the nuclear envelope that is relatively weak and liable to degradation. Third, Higher rate of evolution: DNA alterations (mutations) ...
... Second, Better protection: The mitochondrion also has a strong protein coat that protects the mitochondrial DNA from degradation by bacterial enzymes. This compares to the nuclear envelope that is relatively weak and liable to degradation. Third, Higher rate of evolution: DNA alterations (mutations) ...
Troubling and Terrific Technology
... restriction enzyme, the enzyme cuts it in the same place every time. This leaves the same fragments of DNA every time it is exposed to the restriction enzyme Sticky ends - restriction fragments can be staggered, leaving short stretches of single stranded DNA (sticky ends). These single stranded area ...
... restriction enzyme, the enzyme cuts it in the same place every time. This leaves the same fragments of DNA every time it is exposed to the restriction enzyme Sticky ends - restriction fragments can be staggered, leaving short stretches of single stranded DNA (sticky ends). These single stranded area ...
Exclusive Highly-Specific Kits and Antibodies for DNA
... family of oxygenases. 5-hydroxymethylcytosine may represent a new pathway to demethylate DNA involving a repair mechanism converting hmC to C and, as such open up entirely new perspectives in epigenetic studies. Since its discovery in neuronal Purkinje, granule and ES cells, studies of this new modi ...
... family of oxygenases. 5-hydroxymethylcytosine may represent a new pathway to demethylate DNA involving a repair mechanism converting hmC to C and, as such open up entirely new perspectives in epigenetic studies. Since its discovery in neuronal Purkinje, granule and ES cells, studies of this new modi ...
B6-AB DNA
... organism? What does it look like, how does it work, and where is it found? The Genetic Blueprint for Life Deoxyribonucleic acid (DNA) stores all of the genetic information required to grow and maintain a living organism. In eukaryotes, DNA is housed in the nucleus of each cell. Like other major orga ...
... organism? What does it look like, how does it work, and where is it found? The Genetic Blueprint for Life Deoxyribonucleic acid (DNA) stores all of the genetic information required to grow and maintain a living organism. In eukaryotes, DNA is housed in the nucleus of each cell. Like other major orga ...
DNA repair
DNA repair is a collection of processes by which a cell identifies and corrects damage to the DNA molecules that encode its genome. In human cells, both normal metabolic activities and environmental factors such as UV light and radiation can cause DNA damage, resulting in as many as 1 million individual molecular lesions per cell per day. Many of these lesions cause structural damage to the DNA molecule and can alter or eliminate the cell's ability to transcribe the gene that the affected DNA encodes. Other lesions induce potentially harmful mutations in the cell's genome, which affect the survival of its daughter cells after it undergoes mitosis. As a consequence, the DNA repair process is constantly active as it responds to damage in the DNA structure. When normal repair processes fail, and when cellular apoptosis does not occur, irreparable DNA damage may occur, including double-strand breaks and DNA crosslinkages (interstrand crosslinks or ICLs).The rate of DNA repair is dependent on many factors, including the cell type, the age of the cell, and the extracellular environment. A cell that has accumulated a large amount of DNA damage, or one that no longer effectively repairs damage incurred to its DNA, can enter one of three possible states: an irreversible state of dormancy, known as senescence cell suicide, also known as apoptosis or programmed cell death unregulated cell division, which can lead to the formation of a tumor that is cancerousThe DNA repair ability of a cell is vital to the integrity of its genome and thus to the normal functionality of that organism. Many genes that were initially shown to influence life span have turned out to be involved in DNA damage repair and protection.