LS1a Problem Set #2
... iii. For each product strand, include the primer sequence used to generate it at the 5’ end. iv. Indicate the region(s) that each product contains (i.e., A, B, C, and/or D). For example, a solid-strand spanning region AB would be drawn as: ...
... iii. For each product strand, include the primer sequence used to generate it at the 5’ end. iv. Indicate the region(s) that each product contains (i.e., A, B, C, and/or D). For example, a solid-strand spanning region AB would be drawn as: ...
Section 1: The Structure of DNA
... the double helix unwinds, the two complementary strands of DNA separate from each other and form Y shapes. These Yshaped areas are called replication forks. At the replication fork, new nucleotides are added to each side and new base pairs are formed according to the base-pairing rules. ...
... the double helix unwinds, the two complementary strands of DNA separate from each other and form Y shapes. These Yshaped areas are called replication forks. At the replication fork, new nucleotides are added to each side and new base pairs are formed according to the base-pairing rules. ...
DNA - s3.amazonaws.com
... 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6. Using the chart on page 303, identify the amino acids coded for by these codons: ...
... 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6. Using the chart on page 303, identify the amino acids coded for by these codons: ...
Study Guide Chapter 16- Molecular basis of Inheritance
... 12. How do the following proteins: helicase and single stranded binding proteins assist in replication of DNA? Helicase- unwinds the DNA helix s.s. binding- bind to template DNA strands to keep them single stranded during replication 13. Can DNA Pol III initiate the synthesis of a new DNA strand? If ...
... 12. How do the following proteins: helicase and single stranded binding proteins assist in replication of DNA? Helicase- unwinds the DNA helix s.s. binding- bind to template DNA strands to keep them single stranded during replication 13. Can DNA Pol III initiate the synthesis of a new DNA strand? If ...
Packet #1: DNA Structure and Function
... principle.” The candidate molecules were DNA, RNA, and protein. These molecules were candidates because we knew that nuclei contained chromosomes which are associated with phenotypes (think Morgan’s fruit fly eye color experiments where eye color corresponded to the X- or Y-chromosome content of the ...
... principle.” The candidate molecules were DNA, RNA, and protein. These molecules were candidates because we knew that nuclei contained chromosomes which are associated with phenotypes (think Morgan’s fruit fly eye color experiments where eye color corresponded to the X- or Y-chromosome content of the ...
12.2 Powerpoint
... SWBAT create a timeline of the scientists that discovered DNA. SWBAT label parts of the DNA molecule SWBAT describe how a chromosome coils. ...
... SWBAT create a timeline of the scientists that discovered DNA. SWBAT label parts of the DNA molecule SWBAT describe how a chromosome coils. ...
Ch. 5: Presentation Slides
... • Many restriction enzymes all cleave their restriction site asymmetrically—at a different site on the two DNA strands • They create sticky ends = each end of the cleaved site has a single-stranded overhang that is complementary in base sequence to the other end • Some restriction enzymes cleave sym ...
... • Many restriction enzymes all cleave their restriction site asymmetrically—at a different site on the two DNA strands • They create sticky ends = each end of the cleaved site has a single-stranded overhang that is complementary in base sequence to the other end • Some restriction enzymes cleave sym ...
FINDING DNA
... • James Watson, a biologist from Indiana University, and Francis Crick, a physicist, were working at the Cavendish Lab in Cambridge, England Nobel Prize for structure of DNA ...
... • James Watson, a biologist from Indiana University, and Francis Crick, a physicist, were working at the Cavendish Lab in Cambridge, England Nobel Prize for structure of DNA ...
Sanger Sequencing Simulation
... understanding of how the DNA is duplicated as cells grow and divide. This process of DNA replication was then harnessed as a tool for the Sanger method of determining the sequence of a piece of DNA. Modern DNA sequencing technology is based on the method of controlled interruption of DNA replication ...
... understanding of how the DNA is duplicated as cells grow and divide. This process of DNA replication was then harnessed as a tool for the Sanger method of determining the sequence of a piece of DNA. Modern DNA sequencing technology is based on the method of controlled interruption of DNA replication ...
Unit-IV GENETIC ENGINEERING
... DNA Fingerprinting Technique DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a ...
... DNA Fingerprinting Technique DNA profiling (also called DNA testing, DNA typing, or genetic fingerprinting) is a technique employed by forensic scientists to assist in the identification of individuals by their respective DNA profiles. DNA profiles are encrypted sets of numbers that reflect a ...
A Crash Course in Genetics
... 4) DNA Polymerase, a key replication enzyme, travels along the single DNA strand adding free nucleotides to the 3’ end of the new strand (Directionality of 5’ to 3’). DNA Polymerase also proofreads the newly built strand in progress, checking that the nexly added nucleotide is in fact complementary. ...
... 4) DNA Polymerase, a key replication enzyme, travels along the single DNA strand adding free nucleotides to the 3’ end of the new strand (Directionality of 5’ to 3’). DNA Polymerase also proofreads the newly built strand in progress, checking that the nexly added nucleotide is in fact complementary. ...
New Measurements of DNA Twist Elasticity
... The idea of studying the response of DNA to mechanical stress is as old as the discovery of the double helix structure itself. While many elements of DNA function require detailed understanding of specific chemical bonds (for example the binding of small ligands), still others are quite nonspecific ...
... The idea of studying the response of DNA to mechanical stress is as old as the discovery of the double helix structure itself. While many elements of DNA function require detailed understanding of specific chemical bonds (for example the binding of small ligands), still others are quite nonspecific ...
A -
... of the cell division process. Before a cell divides, it first duplicates its DNA so that the new cell will have the same genetic information. The specific base pair matching during replication ensures that exact DNA copies are made. ...
... of the cell division process. Before a cell divides, it first duplicates its DNA so that the new cell will have the same genetic information. The specific base pair matching during replication ensures that exact DNA copies are made. ...
Test File
... direction of replication. c. The Okazaki fragments are joined by the action of DNA ligase. d. Both strands are synthesized continuously at the replication fork. 14. DNA polymerase requires a primer and cannot initiate synthesis de novo. What serves as a primer for DNA replication? a. Short fragments ...
... direction of replication. c. The Okazaki fragments are joined by the action of DNA ligase. d. Both strands are synthesized continuously at the replication fork. 14. DNA polymerase requires a primer and cannot initiate synthesis de novo. What serves as a primer for DNA replication? a. Short fragments ...
DNA
... 4) DNA Polymerase, a key replication enzyme, travels along the single DNA strand adding free nucleotides to the 3’ end of the new strand (Directionality of 5’ to 3’). DNA Polymerase also proofreads the newly built strand in progress, checking that the nexly added nucleotide is in fact complementary. ...
... 4) DNA Polymerase, a key replication enzyme, travels along the single DNA strand adding free nucleotides to the 3’ end of the new strand (Directionality of 5’ to 3’). DNA Polymerase also proofreads the newly built strand in progress, checking that the nexly added nucleotide is in fact complementary. ...
Lehninger Principles of Biochemistry
... requirements for DNA melting explain why DNA at replication origins, and at promoters used in gene transcription is enriched in A/T base pairs. RNA-RNA double helices and DNA-RNA hybrid double helices melt at higher temperatures than double-helical DNAs of comparable base composition, for unknown re ...
... requirements for DNA melting explain why DNA at replication origins, and at promoters used in gene transcription is enriched in A/T base pairs. RNA-RNA double helices and DNA-RNA hybrid double helices melt at higher temperatures than double-helical DNAs of comparable base composition, for unknown re ...
Day_1_-_DNA
... Okazaki fragment. B. DNA Pol III is released from lagging strand and “snaps back” to nearest unused primer to start new Okazaki fragment. C. DNA polymerase I degrades primer between Okazaki fragments in 5’ to 3’ direction and fills gap with DNA. D. DNA ligase seals last gap. ...
... Okazaki fragment. B. DNA Pol III is released from lagging strand and “snaps back” to nearest unused primer to start new Okazaki fragment. C. DNA polymerase I degrades primer between Okazaki fragments in 5’ to 3’ direction and fills gap with DNA. D. DNA ligase seals last gap. ...
DNA Replication
... DNA – deoxyribonucleic acid is the nucleic acid that stores and transmits genetic info. from one generation to the next. •present in all organisms, but different (unique) in each individual, except for identical twins. ...
... DNA – deoxyribonucleic acid is the nucleic acid that stores and transmits genetic info. from one generation to the next. •present in all organisms, but different (unique) in each individual, except for identical twins. ...
Paper I- Discussion Points
... Remember that In these experiments, we are following the cells by time-lapse fluorescence microscopy as they get out of G1 and go through S phase. We define co-localization as the two loci being at or less than 350 nm from each other for a length of 2 min or more. As noted already, we define the rep ...
... Remember that In these experiments, we are following the cells by time-lapse fluorescence microscopy as they get out of G1 and go through S phase. We define co-localization as the two loci being at or less than 350 nm from each other for a length of 2 min or more. As noted already, we define the rep ...
Chapter 16 Outline
... leading strand requires the formation of only a single primer as the replication fork continues to separate. For synthesis of the lagging strand, each Okazaki fragment must be primed separately. Another DNA polymerase, DNA polymerase I, replaces the RNA nucleotides of the primers with DNA versio ...
... leading strand requires the formation of only a single primer as the replication fork continues to separate. For synthesis of the lagging strand, each Okazaki fragment must be primed separately. Another DNA polymerase, DNA polymerase I, replaces the RNA nucleotides of the primers with DNA versio ...
Unit 5, pt 1: Chapter Objectives: from C Massengale – Biology
... 13. Explain the general process of transcription, including the three major steps of initiation, elongation, and termination. 14. Explain how RNA is modified after transcription in eukaryotic cells. 15. Describe the functional and evolutionary significance of introns. The Synthesis of Protein 16. D ...
... 13. Explain the general process of transcription, including the three major steps of initiation, elongation, and termination. 14. Explain how RNA is modified after transcription in eukaryotic cells. 15. Describe the functional and evolutionary significance of introns. The Synthesis of Protein 16. D ...
Nucleic Acids and the RNA World
... random chance and bonding) Hairpin loops forming a ribozyme (RNA Catalyst) – This molecule can split (through catalysis) and can form a template with free floating nucleotides (through base pairing) – This allows it to rapidly replicate itself, giving it a chance to increase its numbers against the ...
... random chance and bonding) Hairpin loops forming a ribozyme (RNA Catalyst) – This molecule can split (through catalysis) and can form a template with free floating nucleotides (through base pairing) – This allows it to rapidly replicate itself, giving it a chance to increase its numbers against the ...
Eukaryotic DNA replication
Eukaryotic DNA replication is a conserved mechanism that restricts DNA replication to only once per cell cycle. Eukaryotic DNA replication of chromosomal DNA is central for the duplication of a cell and is necessary for the maintenance of the eukaryotic genome.DNA replication is the action of DNA polymerases synthesizing a DNA strand complementary to the original template strand. To synthesize DNA, the double-stranded DNA is unwound by DNA helicases ahead of polymerases, forming a replication fork containing two single-stranded templates. Replication processes permit the copying of a single DNA double helix into two DNA helices, which are divided into the daughter cells at mitosis. The major enzymatic functions carried out at the replication fork are well conserved from prokaryotes to eukaryotes, but the replication machinery in eukaryotic DNA replication is a much larger complex, coordinating many proteins at the site of replication, forming the replisome.The replisome is responsible for copying the entirety of genomic DNA in each proliferative cell. This process allows for the high-fidelity passage of hereditary/genetic information from parental cell to daughter cell and is thus essential to all organisms. Much of the cell cycle is built around ensuring that DNA replication occurs without errors.In G1 phase of the cell cycle, many of the DNA replication regulatory processes are initiated. In eukaryotes, the vast majority of DNA synthesis occurs during S phase of the cell cycle, and the entire genome must be unwound and duplicated to form two daughter copies. During G2, any damaged DNA or replication errors are corrected. Finally, one copy of the genomes is segregated to each daughter cell at mitosis or M phase. These daughter copies each contain one strand from the parental duplex DNA and one nascent antiparallel strand.This mechanism is conserved from prokaryotes to eukaryotes and is known as semiconservative DNA replication. The process of semiconservative replication for the site of DNA replication is a fork-like DNA structure, the replication fork, where the DNA helix is open, or unwound, exposing unpaired DNA nucleotides for recognition and base pairing for the incorporationof free nucleotides into double-stranded DNA.