BAC vectors (Bacterial Artificial Chromosome)
... (here BamH I) remove a length of DNA between two telomere sequences leaving the telomeres at the ends of the linearized DNA. Cleavage at another internal site (EcoRI) divides the vector into two DNA segments, referred to as vector arms, each with a different selectable marker. The genomic DNA is pre ...
... (here BamH I) remove a length of DNA between two telomere sequences leaving the telomeres at the ends of the linearized DNA. Cleavage at another internal site (EcoRI) divides the vector into two DNA segments, referred to as vector arms, each with a different selectable marker. The genomic DNA is pre ...
(DNA).
... • To use PCR, the sequence of a gene to be copied or at least a sequenced segment bordering the desired DNA must be known. • In such a case, two primers that are complementary to the ends of the gene or to the bordering DNA can be synthesized. The primers are polynucleotides consisting of 12 to 16 n ...
... • To use PCR, the sequence of a gene to be copied or at least a sequenced segment bordering the desired DNA must be known. • In such a case, two primers that are complementary to the ends of the gene or to the bordering DNA can be synthesized. The primers are polynucleotides consisting of 12 to 16 n ...
Word Work File L_2.tmp
... hydroxyl at another end. 7. The two DNA strands are antiparallel, that is, their sugar phosphate backbones run in opposite directions. 8. DNA polymerases catalyze the linking together of the nucleotide subunits. There are at least eleven DNA polymerases involved in eukaryote replication. 9. Nucleoti ...
... hydroxyl at another end. 7. The two DNA strands are antiparallel, that is, their sugar phosphate backbones run in opposite directions. 8. DNA polymerases catalyze the linking together of the nucleotide subunits. There are at least eleven DNA polymerases involved in eukaryote replication. 9. Nucleoti ...
Slide 1
... Any two unrelated individuals differ by one base pair every 1,000 or so, referred to as SNPs. Many SNPs have no effect on cell function and therefore can be used as molecular markers. ...
... Any two unrelated individuals differ by one base pair every 1,000 or so, referred to as SNPs. Many SNPs have no effect on cell function and therefore can be used as molecular markers. ...
DNA Extraction - Sucrose Lysis Method
... into the lower chamber. If any wash solution remains in the upper chamber, extend the spin time for subsequent spins. 21. Load up to 4 ml of your sample into the upper reservoir of your pre-rinsed column, and spin at 2000 x g (4°C, 10 min). NOTE: After spinning your sample, there will still be liqui ...
... into the lower chamber. If any wash solution remains in the upper chamber, extend the spin time for subsequent spins. 21. Load up to 4 ml of your sample into the upper reservoir of your pre-rinsed column, and spin at 2000 x g (4°C, 10 min). NOTE: After spinning your sample, there will still be liqui ...
1 What Does DNA Look Like?
... Before a cell divides, it makes a copy of its genetic information for the new cell. The pairing of bases allows the cell to replicate, or make copies of, DNA. Remember that bases are complementary and can only fit together in certain ways. Therefore, the order of bases on one side of the DNA strand ...
... Before a cell divides, it makes a copy of its genetic information for the new cell. The pairing of bases allows the cell to replicate, or make copies of, DNA. Remember that bases are complementary and can only fit together in certain ways. Therefore, the order of bases on one side of the DNA strand ...
Molecular Biology what are proteins? what are the building blocks of
... location - ribosomes in cytoplasm or on ER enzymes - none, uses tRNA - brings amino acids and rRNA - ribosomes to combine amino acids sites: codon - set of 3 nucleotides on mRNA, match up with anticodon - set of 3 nucleotides on tRNA start codon - begin process stop codon - end process binding site ...
... location - ribosomes in cytoplasm or on ER enzymes - none, uses tRNA - brings amino acids and rRNA - ribosomes to combine amino acids sites: codon - set of 3 nucleotides on mRNA, match up with anticodon - set of 3 nucleotides on tRNA start codon - begin process stop codon - end process binding site ...
GLP 021 - University of Newcastle
... Transfer the aqueous phase to a fresh tube, and save the organic phase if isolation of DNA or protein is desired. After complete removal of the aqueous phase, as described in the RNA isolation protocol, the DNA in the interphase and phenol phase from the initial homogenate may be isolated. Following ...
... Transfer the aqueous phase to a fresh tube, and save the organic phase if isolation of DNA or protein is desired. After complete removal of the aqueous phase, as described in the RNA isolation protocol, the DNA in the interphase and phenol phase from the initial homogenate may be isolated. Following ...
Ch. 16 The Molecular Basis of Life
... unpaired strands and keeps them stable until new strands can be synthesized 5. DNA polymerase adds new nucleotides by adding a nucleoside triphosphate. When added it loses two phosphate groups (pyrophosphate) which then gets hydrolyzed to two phosphate molecules DNA polymerase adds the nucleot ...
... unpaired strands and keeps them stable until new strands can be synthesized 5. DNA polymerase adds new nucleotides by adding a nucleoside triphosphate. When added it loses two phosphate groups (pyrophosphate) which then gets hydrolyzed to two phosphate molecules DNA polymerase adds the nucleot ...
Molecular Genetics
... because there are 64 different types of codons, but only 20 amino acids • Takes 3 bases to code for 1 amino acid ...
... because there are 64 different types of codons, but only 20 amino acids • Takes 3 bases to code for 1 amino acid ...
Identification, Individualization, Uniqueness
... others showed that because the effect of the database search is not merely to identify a single suspect, but to exclude the rest of the database as potential suspects, the match actually is at least slightly more probative than a single-suspect match (for citations and a simple proof, see Walsh & Bu ...
... others showed that because the effect of the database search is not merely to identify a single suspect, but to exclude the rest of the database as potential suspects, the match actually is at least slightly more probative than a single-suspect match (for citations and a simple proof, see Walsh & Bu ...
DNA notes
... DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions. Commercial kits are now available for easy PCR reaction setup an ...
... DNA degraded to fragments only a few hundred base pairs in length can serve as effective templates for amplification. Large numbers of copies of specific DNA sequences can be amplified simultaneously with multiplex PCR reactions. Commercial kits are now available for easy PCR reaction setup an ...
No Slide Title
... strands, and then bundled up even more. In a cell that has a nucleus, the strands of DNA and proteins are bundled into chromosomes. • A gene consists of a string of nucleotides that give the cell information about how to make a specific trait. ...
... strands, and then bundled up even more. In a cell that has a nucleus, the strands of DNA and proteins are bundled into chromosomes. • A gene consists of a string of nucleotides that give the cell information about how to make a specific trait. ...
DNA Replication
... strain Pneumoccocus bacteria • He found that R strain could become virulent when it took in DNA from heat-killed S strain • Study suggested that DNA was probably the genetic material ...
... strain Pneumoccocus bacteria • He found that R strain could become virulent when it took in DNA from heat-killed S strain • Study suggested that DNA was probably the genetic material ...
DNA:RNA PACKETPkt_
... 9. State two safety precautions that specifically related to this lab. Explain how you maintained a safe lab ...
... 9. State two safety precautions that specifically related to this lab. Explain how you maintained a safe lab ...
Case Study First Draft: How does DNA replicate
... ‘If an adenine forms one member of a pair, on either chain, then…the other member must be thymine; similarly for guanine and cytosine…It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material. ’ Watson and Cr ...
... ‘If an adenine forms one member of a pair, on either chain, then…the other member must be thymine; similarly for guanine and cytosine…It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material. ’ Watson and Cr ...
DNA Structure - WordPress.com
... a measure of length. 1000 bp = 1 kilobase pair (kb or kbp) 1,000,000 = 1 megabase pair (Mb or Mbp) ...
... a measure of length. 1000 bp = 1 kilobase pair (kb or kbp) 1,000,000 = 1 megabase pair (Mb or Mbp) ...
Chapter 9 DNA Powerpoint
... recovered from bodies or stains that have been subject to extreme decomposition • With the technology of PCR one can extract and amplify a combination of different STR’s. More on ...
... recovered from bodies or stains that have been subject to extreme decomposition • With the technology of PCR one can extract and amplify a combination of different STR’s. More on ...
Mutation detection using nucleotide analogs that alter
... strand consistently causes that DNA to migrate two bases slower than expected in a sequencing gel (16). Prompted by these observations, we have found that incorporation of biotin-11-dUTP, a commercially available analog of TTP, into a DNA strand causes a one nucleotide mobility shift when the DNA is ...
... strand consistently causes that DNA to migrate two bases slower than expected in a sequencing gel (16). Prompted by these observations, we have found that incorporation of biotin-11-dUTP, a commercially available analog of TTP, into a DNA strand causes a one nucleotide mobility shift when the DNA is ...
Structure and function of DNA
... The bases are on the inside of the molecules and the 2 chains are joined together by double H-bond between A and T and triple H-bond between C and G. The base pairing is very specific which make the 2 strands complementary to each other. So each strand contain all the required information for synthe ...
... The bases are on the inside of the molecules and the 2 chains are joined together by double H-bond between A and T and triple H-bond between C and G. The base pairing is very specific which make the 2 strands complementary to each other. So each strand contain all the required information for synthe ...
Structure and function of DNA
... The bases are on the inside of the molecules and the 2 chains are joined together by double H-bond between A and T and triple H-bond between C and G. The base pairing is very specific which make the 2 strands complementary to each other. So each strand contain all the required information for synthe ...
... The bases are on the inside of the molecules and the 2 chains are joined together by double H-bond between A and T and triple H-bond between C and G. The base pairing is very specific which make the 2 strands complementary to each other. So each strand contain all the required information for synthe ...
DNA: Hereditary Molecules of Life
... Negative DNA wraps around positive histones Nucleosome – cluster of 8 histones Solenoids – coiled strings of nucleosomes (chromatin ...
... Negative DNA wraps around positive histones Nucleosome – cluster of 8 histones Solenoids – coiled strings of nucleosomes (chromatin ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.