Technological applications arising from the interactions of DNA
... have been constructed to monitor DNA hybridization and to detect small molecules. The hydrogen bonding pattern in the DNA stem region is replaced by metal-base pairing (Figure 2b) [14,15]. The new molecular beacon was demonstrated to be superior because it exhibits a lower background signal, higher ...
... have been constructed to monitor DNA hybridization and to detect small molecules. The hydrogen bonding pattern in the DNA stem region is replaced by metal-base pairing (Figure 2b) [14,15]. The new molecular beacon was demonstrated to be superior because it exhibits a lower background signal, higher ...
Bacteria Screening PCR Kit
... pH8.0) then suspend in a 200 μl of Chelex® solution. 2) Heat at 99℃ for a period of 5 minutes then quickly cool by placing on ice for 1 minute or more. 3) Centrifuge at 12,000 rpm (approx. 13,000 X g ) for 1 minute. 4) Use the supernatant as the DNA Sample Solution for PCR. [ Option 2 ] (Use o ...
... pH8.0) then suspend in a 200 μl of Chelex® solution. 2) Heat at 99℃ for a period of 5 minutes then quickly cool by placing on ice for 1 minute or more. 3) Centrifuge at 12,000 rpm (approx. 13,000 X g ) for 1 minute. 4) Use the supernatant as the DNA Sample Solution for PCR. [ Option 2 ] (Use o ...
CIRCULAR DNA MOLECULES IN THE GENUS
... strains of D. melanogaster (Canton S , Lausanne, Oregon R-M and Swedish C) were analyzed with the same results. If these results are an artifact of the DNA extraction procedure. it applies only to D. melanogaster, for when DNA is extracted from a mixture of D. uirilis and D. melanogaster embryos, th ...
... strains of D. melanogaster (Canton S , Lausanne, Oregon R-M and Swedish C) were analyzed with the same results. If these results are an artifact of the DNA extraction procedure. it applies only to D. melanogaster, for when DNA is extracted from a mixture of D. uirilis and D. melanogaster embryos, th ...
Sheared DNA fragment sizing: comparison of techniques
... Fig. 4 Size Distribution of Sheared DNA Fragments. Panels a, b and c show the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative m ...
... Fig. 4 Size Distribution of Sheared DNA Fragments. Panels a, b and c show the relative number of DNA molecules corresponding to given length increments (base pairs) as determined by Kleinschmidt EM, Adsorption EM and gel electrophoresis procedures, respectively. Panels d, e and f show the relative m ...
DNA RNA Protein Trait DNA mRNA Protein
... Nucleic Acid Chemistry Automated DNA Sequencing • One major improvement in recent years has been the development of automated procedures for fluorescent DNA sequencing (Wilson et al., Genomics, 1990, pg. 626). • These procedures generally use primers or dideoxynucleotides to which are attached fluo ...
... Nucleic Acid Chemistry Automated DNA Sequencing • One major improvement in recent years has been the development of automated procedures for fluorescent DNA sequencing (Wilson et al., Genomics, 1990, pg. 626). • These procedures generally use primers or dideoxynucleotides to which are attached fluo ...
Document
... 17- Record the charge for each dye molecule by noting which pole (+/-) the dye sample was traveling when the power was shut off. [DATA TABLE] 18- Use the recorded migration distances to complete the last column of the data table by ranking the five known dye samples from fastest (#1) to slowest (#5) ...
... 17- Record the charge for each dye molecule by noting which pole (+/-) the dye sample was traveling when the power was shut off. [DATA TABLE] 18- Use the recorded migration distances to complete the last column of the data table by ranking the five known dye samples from fastest (#1) to slowest (#5) ...
Trawling DNA Databases for Partial Matches: What is the FBI Afraid
... and federal databases as combined in the National DNA Index System (NDIS) hold over seven million short tandem repeat (STR) profiles from convicted offenders as well as a growing number of people who were merely arrested or detained. 3 When investigators recover a DNA sample from the scene of a crim ...
... and federal databases as combined in the National DNA Index System (NDIS) hold over seven million short tandem repeat (STR) profiles from convicted offenders as well as a growing number of people who were merely arrested or detained. 3 When investigators recover a DNA sample from the scene of a crim ...
Stereoselectivity in DNA-Templated Organic
... in stabilizing single-stranded helical conformations. Indeed, singlestranded DNA of mixed sequence has been reported to retain helical structure even when approximately one-half of its aromatic bases are removed.5 This model of stereoselectivity predicts that global conformational changes in the tem ...
... in stabilizing single-stranded helical conformations. Indeed, singlestranded DNA of mixed sequence has been reported to retain helical structure even when approximately one-half of its aromatic bases are removed.5 This model of stereoselectivity predicts that global conformational changes in the tem ...
Application of PCR-technique in biological labs
... • A piece of X-ray film is put on the top and a spot is produced on the film where the probe sticks. • Using a ruler, scientists measure the position of the spots on the film and produce a set of numb ers. • The odds of two individuals having the same pattern are between 1,000 to 1-to trillions- 1 ...
... • A piece of X-ray film is put on the top and a spot is produced on the film where the probe sticks. • Using a ruler, scientists measure the position of the spots on the film and produce a set of numb ers. • The odds of two individuals having the same pattern are between 1,000 to 1-to trillions- 1 ...
Polymerase chain reaction and its applications
... The enzyme was purif|ed in 1958, but automation and modern PCR technology was not developed until 1983. The discovery of thermostable polymerase enzymes revolutionized PCR making automation and rapid reactions possible. PCR mimics the in vivo process of DNA replication. It begins with one molecule o ...
... The enzyme was purif|ed in 1958, but automation and modern PCR technology was not developed until 1983. The discovery of thermostable polymerase enzymes revolutionized PCR making automation and rapid reactions possible. PCR mimics the in vivo process of DNA replication. It begins with one molecule o ...
Laboratory of Insect Genetics and Biosciences (IGB) Dept. Biology
... in bees. From the literature it is obvious that methylation happens, and that different castes have a different methylation profile. However, the text goes too far on suggesting that the origin of eusociality is explained by the acquisition of epigenetic mechanisms. There are many 'traditional genet ...
... in bees. From the literature it is obvious that methylation happens, and that different castes have a different methylation profile. However, the text goes too far on suggesting that the origin of eusociality is explained by the acquisition of epigenetic mechanisms. There are many 'traditional genet ...
DNA Repair: Its Importance and How to Improve it An Interview with
... its DNA, since there is no template available for repair by homologou s recombination. There are “backup” NHEJ pathways in higher eukaryotes. Besides its role as a genome caretaker, NHEJ is required for joining hairpin-capped double-strand breaks induced during V(D)J recombination, the process that ...
... its DNA, since there is no template available for repair by homologou s recombination. There are “backup” NHEJ pathways in higher eukaryotes. Besides its role as a genome caretaker, NHEJ is required for joining hairpin-capped double-strand breaks induced during V(D)J recombination, the process that ...
Vol. 75, No. 5, May 2002 New Laws Reflect the Power and Potential
... postconviction DNA cases the evidence is misplaced or discovered in places where chain of custody may be dubious. It also recognizes that DNA evidence is unique in that often it can itself establish to a high degree of certainty its own authenticity and integrity. In sexual assault cases, for exampl ...
... postconviction DNA cases the evidence is misplaced or discovered in places where chain of custody may be dubious. It also recognizes that DNA evidence is unique in that often it can itself establish to a high degree of certainty its own authenticity and integrity. In sexual assault cases, for exampl ...
Leading strand
... nucleotides are added. – DNA polymerase III extends the strand in the 5’-to-3’ direction – DNA polymerase I degrades the RNA primer and replaces it with DNA – DNA ligase joins the DNA fragments into a continuous daughter strand Overall direction of replication ...
... nucleotides are added. – DNA polymerase III extends the strand in the 5’-to-3’ direction – DNA polymerase I degrades the RNA primer and replaces it with DNA – DNA ligase joins the DNA fragments into a continuous daughter strand Overall direction of replication ...
Interaction of Rat Testis Protein, TP, with Nucleic Acids in Vitro
... respectively. It is clear that TP did induce hyperchromicity of both DNA (Fig. 8A) and poly(rA) (Fig. 8B) at this salt concentration also, and the shapes of the difference spectra were very similar to those observed at 2 mM Na' concentration. Effect of TP Binding on the Thermal Melting of the DNAThe ...
... respectively. It is clear that TP did induce hyperchromicity of both DNA (Fig. 8A) and poly(rA) (Fig. 8B) at this salt concentration also, and the shapes of the difference spectra were very similar to those observed at 2 mM Na' concentration. Effect of TP Binding on the Thermal Melting of the DNAThe ...
Chem 121 Chapter 22. Nucleic Acids 1. Any given nucleotide in a
... D) base pairing combinations are always A-C and G-T 8. Replication of DNA produces two daughter DNA molecules in which A) one daughter molecule contains both parent strands and one daughter molecule contains both newly synthesized strands. B) each daughter molecule contains one parent strand and one ...
... D) base pairing combinations are always A-C and G-T 8. Replication of DNA produces two daughter DNA molecules in which A) one daughter molecule contains both parent strands and one daughter molecule contains both newly synthesized strands. B) each daughter molecule contains one parent strand and one ...
7.03 Fall 2003 Problem Set #3 Solutions
... never co-transduced with lac2+. This indicates that the distance between lac2- and Tn5 is at least one phage head (105 bp). We know from part (a) that Tn5 and lac1- are within one phage head since their cotransduction frequency was 18%. But since we do not know the relative order of the three marker ...
... never co-transduced with lac2+. This indicates that the distance between lac2- and Tn5 is at least one phage head (105 bp). We know from part (a) that Tn5 and lac1- are within one phage head since their cotransduction frequency was 18%. But since we do not know the relative order of the three marker ...
Stalking the Wild Plasmid - Marine Biological Laboratory
... computationally. Many of the plasmid preps yielded a band around 2000 base pairs which, when sequenced, aligned perfectly with the transposon, indicating its circularization (Fig 7a). Unfortunately, this component of the reactions was highly representative in out plasmid library. The fact that the c ...
... computationally. Many of the plasmid preps yielded a band around 2000 base pairs which, when sequenced, aligned perfectly with the transposon, indicating its circularization (Fig 7a). Unfortunately, this component of the reactions was highly representative in out plasmid library. The fact that the c ...
DNA Repair: Its Importance and How to Improve it
... its DNA, since there is no template available for repair by homologous recombination. There are ―backup‖ NHEJ pathways in higher eukaryotes. Besides its role as a genome caretaker, NHEJ is required for joining hairpin-capped double-strand breaks induced during V(D)J recombination, the process that ...
... its DNA, since there is no template available for repair by homologous recombination. There are ―backup‖ NHEJ pathways in higher eukaryotes. Besides its role as a genome caretaker, NHEJ is required for joining hairpin-capped double-strand breaks induced during V(D)J recombination, the process that ...
Lab 1 genomic DNA
... Two common reducing agents found in extraction buffers are ß-mercaptoethanol or dithiothreitol (DTT). EDTA is also present to chelate Mg+2 ions thus mediating aggregation of nucleic acids to each other and to proteins. Ethanol and/or Isopropanol Precipitation ...
... Two common reducing agents found in extraction buffers are ß-mercaptoethanol or dithiothreitol (DTT). EDTA is also present to chelate Mg+2 ions thus mediating aggregation of nucleic acids to each other and to proteins. Ethanol and/or Isopropanol Precipitation ...
Laboratory 9: Plasmid Isolation
... 1. The day before lab, pick 4 transformants from each ligation with separate sterile toothpicks, and inoculate separate culture tubes containing liquid medium (LB+Kan). 2. Grow overnight with shaking at 37ºC. Day of lab: B. Harvest and lyse bacteria. 1. Transfer 1.4 mL of bacterial cultures to a ste ...
... 1. The day before lab, pick 4 transformants from each ligation with separate sterile toothpicks, and inoculate separate culture tubes containing liquid medium (LB+Kan). 2. Grow overnight with shaking at 37ºC. Day of lab: B. Harvest and lyse bacteria. 1. Transfer 1.4 mL of bacterial cultures to a ste ...
The Occurrence of 6-Methylaminopurine in Deoxyribonucleic Acids
... soon after growth ceased. In other cultures, where incubation of the bacteria was continued for several hours after growths had ceased, the yields of DNA were exceptionally low. The optimum conditions for this type of growth have not yet been determined. ...
... soon after growth ceased. In other cultures, where incubation of the bacteria was continued for several hours after growths had ceased, the yields of DNA were exceptionally low. The optimum conditions for this type of growth have not yet been determined. ...
DNA profiling
DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.