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Chapter 1: Forensic Biology - California State University, Sacramento
Chapter 1: Forensic Biology - California State University, Sacramento

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... 1. You are analyzing a double-stranded DNA sample, and you find that 23% of the sample is guanine (G) nucleotides. What are the percentages of the other three bases? In double-stranded DNA, G pairs with C, so the amounts of these two bases should be equal: C=23% Now, G+C = 46%, so the remaining 54% ...
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... with great inter-locus and intra-color balance. Peak height balances were greater than 70% and there were few occurrences of off-scale data. The data quality of this method was comparable to the method currently used at the USACIL DNA Database; however, with the Investigator® STR GO! Lysis Buffer me ...
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DNA profiling



DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing. First developed and used in 1985, DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic (""identical"") twins. DNA profiling uses repetitive (""repeat"") sequences that are highly variable, called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.The DNA profiling technique nowadays used is based on technology developed in 1988.
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