Presentation
... Multialignments • Select BLAST alignments to be multialigned • Clustal-W performs multialignment • Aligns – The originating IMAS gene sequence – The “Full” sequence found by BLAST • Not just the high-quality section – Useful to align entire genes, or entire corresponding segments of DNA ...
... Multialignments • Select BLAST alignments to be multialigned • Clustal-W performs multialignment • Aligns – The originating IMAS gene sequence – The “Full” sequence found by BLAST • Not just the high-quality section – Useful to align entire genes, or entire corresponding segments of DNA ...
Resolution of the type material of the Asian elephant, Elephas
... control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, us ...
... control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, us ...
Resolution of the type material of the Asian elephant, Elephas
... control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, us ...
... control region) were aligned for 653 Loxodonta (GenBank accession numbers: JQ438119–JQ438771; Ishida et al., 2013) and 73 E. maximus (unpubl. data). Three short regions that contained genus-diagnostic nucleotide sites were identified, and primers were designed with conserved sequences at 3′ ends, us ...
STRUCTURE AND DIAGNOSTIC APPLICATIONS OF DNA
... What type of bonds link the Deoxy-Nucleotides in DNA? ...
... What type of bonds link the Deoxy-Nucleotides in DNA? ...
MOTIFS MOTIFSMARTIFAMORIFSMOOTIFSMICIFC
... The Gold Bug Problem: the ;48 clue • Mapping “the” to “;48” and substituting all occurrences of the symbols: 53++!305))6*the26)h+.)h+)te06*the!e`60))e5t]e*:+*e!e3(ee)5* !th6(tee*96*?te)*+(the5)t5*!2:*+(th956*2(5*h)e`e*th0692e5)t )6!e)h++t1(+9the0e1te:e+1the!e5th)he5!52ee06*e1(+9thet(eeth ...
... The Gold Bug Problem: the ;48 clue • Mapping “the” to “;48” and substituting all occurrences of the symbols: 53++!305))6*the26)h+.)h+)te06*the!e`60))e5t]e*:+*e!e3(ee)5* !th6(tee*96*?te)*+(the5)t5*!2:*+(th956*2(5*h)e`e*th0692e5)t )6!e)h++t1(+9the0e1te:e+1the!e5th)he5!52ee06*e1(+9thet(eeth ...
Structure-based prediction of C2H2 zinc-finger
... in a similar fashion (46–48), one solution is to use a protein–DNA complex of a structurally homologous protein as a template for a TF for which no DNAbound complex is available. Recent papers by Morozov et al. (42) and Contreras-Moreira and Collado-Vides (35) have used all-atom modeling and knowled ...
... in a similar fashion (46–48), one solution is to use a protein–DNA complex of a structurally homologous protein as a template for a TF for which no DNAbound complex is available. Recent papers by Morozov et al. (42) and Contreras-Moreira and Collado-Vides (35) have used all-atom modeling and knowled ...
Original Article:
... Staphylococcus aureus is a gram-positive bacterium infections causing a wide range of diseases, including skin infections, soft tissue infections, endocarditis, and septicaemia. Bacteraemic infections often develop from the resident S. aureus flora located in the anterior nares of the patient. The u ...
... Staphylococcus aureus is a gram-positive bacterium infections causing a wide range of diseases, including skin infections, soft tissue infections, endocarditis, and septicaemia. Bacteraemic infections often develop from the resident S. aureus flora located in the anterior nares of the patient. The u ...
achondroplasia
... • In more than 80 percent of cases, however, achondroplasia is not inherited but results from a new mutation (change) that occurred in the egg or sperm cell that formed the embryo. The parents of children with achondroplasia resulting from new mutations are usually normal-sized. Typically, these par ...
... • In more than 80 percent of cases, however, achondroplasia is not inherited but results from a new mutation (change) that occurred in the egg or sperm cell that formed the embryo. The parents of children with achondroplasia resulting from new mutations are usually normal-sized. Typically, these par ...
Organization of DNA replication origins in the fission yeast genome
... activity. (A) Northern analysis of nmt1⫹ expression in S.pombe wildtype cells (wt) without thiamine (–T) or with 5 μg/ml thiamine (⫹T). The autoradiograph on the left was exposed for 1 h. The right panel shows that a low level of expression was detected in wild-type cells at longer exposure times (4 ...
... activity. (A) Northern analysis of nmt1⫹ expression in S.pombe wildtype cells (wt) without thiamine (–T) or with 5 μg/ml thiamine (⫹T). The autoradiograph on the left was exposed for 1 h. The right panel shows that a low level of expression was detected in wild-type cells at longer exposure times (4 ...
DNA is the genetic material
... At the end of each replication bubble is a replication fork, a Y-shaped region where new DNA strands are elongating Helicase untwists the parental double helix and separates the template DNA strands at the replication fork. This untwisting causes tighter twisting ahead of the replication fork, and t ...
... At the end of each replication bubble is a replication fork, a Y-shaped region where new DNA strands are elongating Helicase untwists the parental double helix and separates the template DNA strands at the replication fork. This untwisting causes tighter twisting ahead of the replication fork, and t ...
Potassium hydroxide - Sigma
... analyses have been performed on the SigmaUltra potassium hydroxide. The Certificate of Analysis provides lot-specific results. SigmaUltra potassium hydroxide is for applications which require tight control of elemental content. Potassium hydroxide (KOH) is a caustic reagent that is widely used to ne ...
... analyses have been performed on the SigmaUltra potassium hydroxide. The Certificate of Analysis provides lot-specific results. SigmaUltra potassium hydroxide is for applications which require tight control of elemental content. Potassium hydroxide (KOH) is a caustic reagent that is widely used to ne ...
Assessing the Homogeneity of Plasmid DNA: An Important
... Plasmids are extra-chromosomal, double-stranded DNA molecules that may exist in several forms differing in topology and size. However, as plasmid DNA is being used as vectors for therapeutic genes, the development of good analytical processes to assess both purity and heterogeneity is of great impor ...
... Plasmids are extra-chromosomal, double-stranded DNA molecules that may exist in several forms differing in topology and size. However, as plasmid DNA is being used as vectors for therapeutic genes, the development of good analytical processes to assess both purity and heterogeneity is of great impor ...
Gel Electrophoresis
... cleave DNA at specific sites along the molecule. They can be seen as scissors for DNA that only cut if they detect a specific order of base pairs. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. Restriction enzymes can be isolated from bacterial c ...
... cleave DNA at specific sites along the molecule. They can be seen as scissors for DNA that only cut if they detect a specific order of base pairs. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. Restriction enzymes can be isolated from bacterial c ...
Phylogenetic Relationships Among Ascomycetes: Evidence from an
... completely (fig. 1; Berbee and Taylor 1995; Gargas and Taylor 1995; Spatafora 1995; Berbee 1996). To achieve a more complete view of the evolutionary relationships between groups of the Ascomycota, it will be necessary to broaden the base of molecular characters used. Protein-coding genes are promis ...
... completely (fig. 1; Berbee and Taylor 1995; Gargas and Taylor 1995; Spatafora 1995; Berbee 1996). To achieve a more complete view of the evolutionary relationships between groups of the Ascomycota, it will be necessary to broaden the base of molecular characters used. Protein-coding genes are promis ...
DNA Double Helix
... DNA and separate the DNA strands as a template to assemble the nucleotides into another DNA strand This first process is called transcription. The process begins at a section of DNA called a promoter, which has specific base sequence that signal Pearson Prentice Hall where Copyright to start ...
... DNA and separate the DNA strands as a template to assemble the nucleotides into another DNA strand This first process is called transcription. The process begins at a section of DNA called a promoter, which has specific base sequence that signal Pearson Prentice Hall where Copyright to start ...
district court, county of boulder, state of colorado
... Then, in January of 1999, Agent Lobato performed more tests on the samples. This time she used a PCR-based multiplex STR system using two commercial kits manufactured by Perkins Elmer Biosytems (PE), the AmpFLSTR Profiler Plus™(Profiler Plus) and the AmpFLSTR Cofiler™ (Cofiler) kits. These kits had ...
... Then, in January of 1999, Agent Lobato performed more tests on the samples. This time she used a PCR-based multiplex STR system using two commercial kits manufactured by Perkins Elmer Biosytems (PE), the AmpFLSTR Profiler Plus™(Profiler Plus) and the AmpFLSTR Cofiler™ (Cofiler) kits. These kits had ...
Bioinformatics Dr. Víctor Treviño Pabellón Tec
... Procedure for comparing two (pair-wise alignment) or more (multiple sequence alignment) sequences by searching for similar patterns that are in the same order in the sequences ...
... Procedure for comparing two (pair-wise alignment) or more (multiple sequence alignment) sequences by searching for similar patterns that are in the same order in the sequences ...
The splicing factor SR45 affects the RNA
... We further analyzed the progression of FWA de novo methylation across subsequent generations in sr45-1. In wild type, the FWA transgene did not reach full levels of methylation until the T2 generation (Fig. 2). In sr45-1 mutants, the levels of methylation at FWA transgene are reduced in the T1 gener ...
... We further analyzed the progression of FWA de novo methylation across subsequent generations in sr45-1. In wild type, the FWA transgene did not reach full levels of methylation until the T2 generation (Fig. 2). In sr45-1 mutants, the levels of methylation at FWA transgene are reduced in the T1 gener ...
Methods for pattern discovery in unaligned biological sequences
... Such patterns could correspond to regions of the sequences responsible for the function itself, and could be used later for the functional annotation of newly determined sequences. Despite many studies, this problem can be considered far from being solved. The main dif®culty lies in the fact that si ...
... Such patterns could correspond to regions of the sequences responsible for the function itself, and could be used later for the functional annotation of newly determined sequences. Despite many studies, this problem can be considered far from being solved. The main dif®culty lies in the fact that si ...
Synonymous codon bias and functional constraint on GC3
... and non-synonymous sites (17) suggesting that evolutionary forces acting on these neighboring sites are not as independent as has often been historically assumed. Intriguingly, a recent experiment in Methylobacterium extorquens demonstrated that synonymous mutations toward globally favored frequent ...
... and non-synonymous sites (17) suggesting that evolutionary forces acting on these neighboring sites are not as independent as has often been historically assumed. Intriguingly, a recent experiment in Methylobacterium extorquens demonstrated that synonymous mutations toward globally favored frequent ...
Identification and quantification of mycotoxigenic fungi
... extended to equal the number of kb of the product to be amplified and to yield doubled DNA. The process is cycled approximately 40 times to give the desired quantity of DNA product. The use of the thermophilic polymerases involved in the reactions is of course revolutionary. The DNAs are separated o ...
... extended to equal the number of kb of the product to be amplified and to yield doubled DNA. The process is cycled approximately 40 times to give the desired quantity of DNA product. The use of the thermophilic polymerases involved in the reactions is of course revolutionary. The DNAs are separated o ...
physical maps
... Programs for identifying matches between a particular sequence and a large population of previously sequenced fragments Programs for identifying overlaps of DNA fragments Programs for estimating error rates Programs for identifying genes in chromosomal sequences Copyright © The McGraw-Hill Companies ...
... Programs for identifying matches between a particular sequence and a large population of previously sequenced fragments Programs for identifying overlaps of DNA fragments Programs for estimating error rates Programs for identifying genes in chromosomal sequences Copyright © The McGraw-Hill Companies ...
Sequence and RFLP analysis of the elongation factor Tu gene used
... 79-9-81.8 % A or T at the third position. Glutamine, for example, is only represented by the codon GAA but never by GAG. From the possible 61 codons, only 45 are used. The GTP-binding elongation factor signature was found in all three sequences between amino acids 16 and 30 (50 and 64 in M. hominis) ...
... 79-9-81.8 % A or T at the third position. Glutamine, for example, is only represented by the codon GAA but never by GAG. From the possible 61 codons, only 45 are used. The GTP-binding elongation factor signature was found in all three sequences between amino acids 16 and 30 (50 and 64 in M. hominis) ...
Nonenzymatic Sequence-Specific Cleavage of Single
... products of the )'P-labeled strand were separated by denaturing polyacrylamide gel electrophoresis and visualized by autorddiography. Cleavage was produced on the complementary strand a t a single G located two base pairs to the 5'-side of the MT-U base (Figure 3. lane 3). Neighboring G bases were c ...
... products of the )'P-labeled strand were separated by denaturing polyacrylamide gel electrophoresis and visualized by autorddiography. Cleavage was produced on the complementary strand a t a single G located two base pairs to the 5'-side of the MT-U base (Figure 3. lane 3). Neighboring G bases were c ...
Contig annotation tool CAT robustly classifies assembled
... questions in metagenomics (Handelsman, 2004) i.e. "Who is there?" and "What are they doing?". Read mapping considers each read as an independent observation, whose taxonomic origin and functional class can be estimated by identifying the closest match in a reference database, and tallying these ann ...
... questions in metagenomics (Handelsman, 2004) i.e. "Who is there?" and "What are they doing?". Read mapping considers each read as an independent observation, whose taxonomic origin and functional class can be estimated by identifying the closest match in a reference database, and tallying these ann ...
Microsatellite
A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.