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Chapter 12: Genetic Engineering
Chapter 12: Genetic Engineering

... code for protein – in the human genome  Junk DNA is made up of repeated sequences that are called repeats  Although individuals may have identical genes, there may be different numbers of repeats between these genes  The more repeats, the longer the junk DNA between genes Restriction enzymes are ...
Ch. 12.2: Replication of DNA
Ch. 12.2: Replication of DNA

... Replication of DNA • Before a cell can divide by mitosis or meiosis, it must first make a copy of its chromosomes. • The DNA in the chromosomes is copied in a process called DNA replication. • Without DNA replication, new cells would have only half the DNA of their parents. • DNA is copied during in ...
Breeding Studies On Tomato For Nematode Resistance Through
Breeding Studies On Tomato For Nematode Resistance Through

... However, most of these marker systems show a low level of polymorphism in wheat, especially among cultivated lines and/or cultivars (Chao et al. 1989; Devos and Gale 1992). ...
12-1Discovering the role of DNA
12-1Discovering the role of DNA

... Prokaryotes – single circular DNA – referred to as chromosome Eukaryotic – DNA inside nucleus in the form of number of chromosomes which varies from one species to next. Eukaryotic chromosome contains DNA and protein tightly packed together to form chromatin in which DNA is coiled around proteins ca ...
Introduction to Genetics
Introduction to Genetics

... Because both strands are copied during PCR, there is an exponential increase of the number of copies of the gene. Suppose there is only one copy of the wanted gene before the cycling starts, after one cycle, there will be 2 copies, after two cycles, there will be 4 copies, three cycles will result ...
document
document

... Southern blot analysis for the diagnosis of fragile X syndrome. Patient DNA is simultaneously digested with restriction endonucleases EcoR1 and Eag1, blotted to a nylon membrane, and hybridized with a 32P-labeled probe adjacent to exon 1 of FMR1 (see Figure 29.1). Eag1 is a methylation-sensitive res ...
mutations
mutations

... “The (achondroplasia) mutations just discussed are single base substitutions. The most striking is achondroplasia, in which 153 of 154 analysed cases are due to a glycine to arginine substitution at codon 1,138. The mutations are in the transmembrane domain of the fibroblast growth factor receptor 3 ...
DNA - Mr. McVey`s Biology Class
DNA - Mr. McVey`s Biology Class

... In simple terms, DNA controls the production of proteins within the cell. These proteins in turn, form the structural units of cells and control all chemical processes within the cell. Think of proteins as the building blocks for an organism. Proteins make up your skin, your hair, and parts of indiv ...
Read Francis Crick reading
Read Francis Crick reading

... Now we have two of these chains winding round each other — each one is a helix — and the chain, made up of sugar and phosphorus, is on the outside, and the bases are all on the inside. I can’t draw it very well, but it looks like this. ...
Nucleic Acids
Nucleic Acids

... Histones – proteins used to wrap the loose strands of chromatin around and start to spool it into chromosomes Nucleosome – unit of several histones with ...
DNA/RNA/Protein Synthesis Replication Cell Division
DNA/RNA/Protein Synthesis Replication Cell Division

... Griffith “F” for failing to find genetic material! But… He found TRANSFORMATION Griffith injected mice with heat killed disease causing bacteria and alive good bacteria. The mice died of pneumonia. Some “factor” caused good bacteria to become disease causing bacteria. This is called TRANSFORMATION! ...
mutated
mutated

... Damage may occur at any time in any cell. Errors during chromosome replication happen only about once in 100,000 bases. Given that the human genome has about 6 billion bases, this means each replication cycle will have 60,000 errors associated with it. Cells contain several complex systems to fix da ...
DNAandReplication Exam Qs.doc
DNAandReplication Exam Qs.doc

... spelling variants offered for thymine. E4.(a) This proved to be a good discriminator. Most students were aware that DNA helicase separates strands or breaks hydrogen bonds. However, only better responses went on to state that this allows nucleotides to attach or the strands to act as templates. Unfo ...
Lecture 8
Lecture 8

... sequence homology. Exchange may can occur at any point between the homologous region, although particular DNA sequences may influence frequency of exchange. 2. Efficiency: whenever sufficiently long homologous sequences are brought together in a single cell under appropriate conditions, the producti ...
02HYD16_Layout 1
02HYD16_Layout 1

... A) Its charged phosphate groups B) The formation of hydrogen bonds between bases from opposite strands C) The opposite direction of the two strands D) The pairing of bases on one strand with bases on the other strand 25. What is antisense technology? A) RNA polymerase producing DNA B) A cell display ...
F1C - OIE
F1C - OIE

... Advantages: Specific, sensitive, high throughput, fast, cost effective, high ...
DNA Replication - inetTeacher.com
DNA Replication - inetTeacher.com

... The DNA Polymerase serves as a proof reader to correct any mistakes Mistakes occur in 1/10,000 nucleotides added. An error creating a DNA could cause a disease to occur or the wrong directions given in another part of the cell. Fun Fact… There are some people who lack DNA polymerase and do not have ...
3_DNA coloring and questions
3_DNA coloring and questions

... center" because it controls all the activities of the cell including cell reproduction, and heredity. How does it do this? The nucleus controls these activities by the chromosomes. Chromosomes are microscopic, threadlike strands composed of the chemical DNA (short for deoxyribonucleic acid). In simp ...
Dog DNA Activity, Part1 and Part 2 Powerpoint
Dog DNA Activity, Part1 and Part 2 Powerpoint

...  The DNA molecule contains a sequence of four chemical bases (represented here by four symbols). Each base is referred to by the first letter of its name: Adenine (A), Cytosine (C), Guanine (G) and Thymine (T).  The sequence of these chemical bases encodes a detailed set of instructions for buildi ...
DNA - Edmonds
DNA - Edmonds

... • Goal: build a simple model of DNA to use as we learn how DNA is transcribed into mRNA and translated into proteins – DNA  mRNA  Proteins  Characteristics • Work with your lab partner at your desk – Complete the “DNA model” instructions • One partner gets materials; other reads instructions – Ma ...
Lecture 6
Lecture 6

... • Rates  vary  widely  for  different   proteins  but  scale  with  time • Local  clock  vs  global  clock • Rates  can  vary  over  branches  and   over  time • Selection • Generation  time  effect • Efficiency  of  DNA  repair • Some  evidence ...
Strings: Theory, Properties and Applications
Strings: Theory, Properties and Applications

... A phylogenetic tree or evolutionary tree is a diagram that depicts the evolutionary relationships among various species or other entities, based upon similarities and differences in their characteristics. Phylogenetic trees can be inferred from sequence alignment and used to detect potentially impor ...
09-DNA-Replication
09-DNA-Replication

...  The two DNA strands are held together by weak hydrogen bonds between complementary base pairs  A and T  C and G ...
DNA - My Teacher Pages
DNA - My Teacher Pages

... • DNA is a polymer made of repeating subunits called nucleotides.(the monomer) • Nucleotides have three parts: a simple sugar, a phosphate group, and a nitrogenous base. Nitrogenous base ...
Plant Nuclear Genome Size Variation
Plant Nuclear Genome Size Variation

... 4)Interspecific variation in mutational tendency to delete excess DNA. 5)Population size and mutational hazard of excess DNA, especially gain of function mutations. 6)All of the above ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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