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Biotechnology Article1
Biotechnology Article1

... Mendel discovered that the inheritance of characteristics in pea plants was governed by some simple rules. Following Mendel, other scientists expanded the study of heredity to include all kinds of living organisms. The scientific study of heredity led to more predictable results in breeding animals ...
The nucleotide sequence of the tnpA gene completes the sequence
The nucleotide sequence of the tnpA gene completes the sequence

... transposase of transposon Tn501 has been determined. It contains an open reading frame for a polypeptide of M -111,500, which terminates within the inverted repeat sequence of the transposon. The reading frame would be transcribed in the same direction as the mercury-resistance genes and the tnpR ge ...
dna sequencing lab - Georgia Standards
dna sequencing lab - Georgia Standards

... How does DNA code for living organisms? How can a change in the sequence of DNA affect the type of protein that is coded? Why would two different species have similar DNA and yet have different traits? Why are the processes of translation and transcription crucial to biological function in an organi ...
DNA! - Chapter 10
DNA! - Chapter 10

... 5. What are four key differences between DNA polymerase and RNA polymerase? (“they are difference molecules” doesn’t count as one!) 6. Compare and contrast codons and anticodons? 7. What is alternative splicing? Why is it necessary in eukaryotes? 8. During translation, what amino acid sequence would ...
File
File

... Highly repetitive DNA accounts for 5%–45% of the human genome. They usually contain 5–300 base pairs that can repeat up to 100,000 times. The function of these sequences is still being researched, but it has been determined that they are transposable (can move from one location to another within the ...
DNA Extraction from Fruit - DNALC Lab Center
DNA Extraction from Fruit - DNALC Lab Center

... • Discuss how James Watson and Francis Crick were able to determine the shape of the DNA molecule. • Discuss the fact that the DNA extraction is an experiment that is not only used by scientists, but also by detectives (fingerprinting) and doctors (disease diagnosis). Why would someone want to extra ...
Gene Prediction in Genomic Studies Ab-initio
Gene Prediction in Genomic Studies Ab-initio

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Chapter 14 Protein Synthesis
Chapter 14 Protein Synthesis

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Molecular Mechanism of Mutation
Molecular Mechanism of Mutation

...  2nd major class of gene mutation  Addition or the removal, respectively, of one or more nucleotide pair  Usually changes the reading frame, altering all amino acids encoded by codons following the mutation  Also called as frame shift mutations ...
issues on dna and informed consent
issues on dna and informed consent

... At this time, NIH, Office for Human Research Protection (OHRP) does not have any plans to add any new regulations. However, on April 17, 1996 OHRP issued a flow chart intended to clarify the applicability of four sections of the Department of Health and Human Services (DHHS) human subject regulation ...
aps6-artifact - Clemson University
aps6-artifact - Clemson University

... For the mRNA code to be translated correctly, codons must be read in the right order. o A change in the order the codons are read changes the resulting protein. ...
C - bYTEBoss
C - bYTEBoss

... manufacture RNA and are concerned with protein synthesis. • Chromosomes are long threads made of DNA and some protein. They are the carriers of GENES which are responsible for our hereditary traits. When a cell Is not dividing the chromosomes are not visible but are in a very tangled mass, the ...
Structure of Nucleic Acids
Structure of Nucleic Acids

... Notice that in this nucleotide the sugar (β-D-ribose, found in RNA) is an aldopentose. If you wanted to draw the sugar found in DNA, then you have to remove the Oxygen atom from C-2’ (keep in mind that the C atoms in the sugar are numbered in a clockwise direction). ...
Supporting online material for
Supporting online material for

... where Br is the bending rigidity, Sr is the stretch modulus, Cr is the twist rigidity, and gr is the twist-stretch coupling. Yr is the Young’s modulus of the material that makes up the inner rod and Rr is the rod’s radius. Note that the inner rod by itself cannot have any twist-stretch coupling (gr ...
(CH7) DNA Repair
(CH7) DNA Repair

... Insertions or deletions can cause frameshift mutations • If the length of an insertion or deletion is not an exact multiple of three nucleotides, this results in a shift in the reading frame of the resulting mRNA. • Usually leads to production of a nonfunctional protein. ...
CHAPTER 10 TEST REVIEW - Hudson City School District
CHAPTER 10 TEST REVIEW - Hudson City School District

... #6 Which enzyme? • Separates the 2 DNA strands (breaks H bonds for replication) • ANSWER: Helicase (D) ...
G. SANTANGELO (*) MACRONUCLEAR DNA CONTENT IN
G. SANTANGELO (*) MACRONUCLEAR DNA CONTENT IN

... All these findings are evidence to the large macronuclear DNA content of heterotrichous ciliates as well as B. americanum and B. japonicum; the large DNA quantities found could be connected with the polyploid, or rather, highly repetitive structure of the macronucleus of ciliates in which many isola ...
GenomeLab™ GeXP Genetic Analysis System
GenomeLab™ GeXP Genetic Analysis System

... The multiplex reaction contains the cDNA for all genes of choice tagged with a 5’ end universal sequence. Two types of primers are present in the reaction: 1) Chimeric primers contain a gene-specific sequence with a tag at the 5’ end universal sequence. They are used to synthesize a double-stranded ...
RNA and Transcription
RNA and Transcription

... know that DNA is the genetic material and its sequence of nucleotide bases carry some sort of code. This code holds instructions that tell a cell how to build proteins, which in turn help to determine an organism’s characteristics. ...
DNA - speringbio
DNA - speringbio

... • The amino acid sequence is aspartic acid-cysteineglycine-leucine-valine ...
DNA profiling : standardising the report
DNA profiling : standardising the report

... data which an independent scientist would be able to use to assess the accuracy of the conclusions drawn in the first section. Variable number tandem repeat (VNTR) probes hybridised under high stringency (single locus) appears to be the technique most favoured in DNA profiling (Budowle et al. 1988). ...
No Slide Title
No Slide Title

... F.J. ...
Figure 11.7
Figure 11.7

... shorter with each round of DNA replication if not solved • Solution= adding DNA sequences to the ends of telomeres • Requires a specialized mechanism catalyzed by the enzyme telomerase (e.g. stem cells, cancer) • Telomerase contains protein and RNA – The RNA is complementary to the DNA sequence foun ...
DNA Replication
DNA Replication

... shorter with each round of DNA replication if not solved • Solution= adding DNA sequences to the ends of telomeres • Requires a specialized mechanism catalyzed by the enzyme telomerase (e.g. stem cells, cancer) • Telomerase contains protein and RNA – The RNA is complementary to the DNA sequence foun ...
Problems 10
Problems 10

... B) (6 pts) No. Any combination of 1-2-6-7 has two centromeres; 5-3-4-7 has three telomeres; segment 7 appears twice. C) (8 pts) Yes. In a heterozygote, during meiosis, translocated chromosomes and normal chromosomes pair. Adjacent I segregation (or independent assortment) results in aneuploidy for s ...
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Microsatellite



A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from 2–5 base pairs) are repeated, typically 5-50 times. Microsatellites occur at thousands of locations in the human genome and they are notable for their high mutation rate and high diversity in the population. Microsatellites and their longer cousins, the minisatellites, together are classified as VNTR (variable number of tandem repeats) DNA. The name ""satellite"" refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying ""satellite"" layers of repetitive DNA. Microsatellites are often referred to as short tandem repeats (STRs) by forensic geneticists, or as simple sequence repeats (SSRs) by plant geneticists.They are widely used for DNA profiling in kinship analysis and in forensic identification. They are also used in genetic linkage analysis/marker assisted selection to locate a gene or a mutation responsible for a given trait or disease.
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