An ISI-like element is responsible for high-level
... P-Lactamase TEM-6 was first detected in two E. coli strains isolated in the Federal Republic of Germany (Bauernfeind & Horl, 1987). Since 1987, outbreaks of infections due to enterobacteria resistant to ceftazidime have been observed in this country (Bauernfeind et al., 1989). Nine transconjugants i ...
... P-Lactamase TEM-6 was first detected in two E. coli strains isolated in the Federal Republic of Germany (Bauernfeind & Horl, 1987). Since 1987, outbreaks of infections due to enterobacteria resistant to ceftazidime have been observed in this country (Bauernfeind et al., 1989). Nine transconjugants i ...
Ch. 12 DNA Replication and Recombination
... Replication are similar but there are some differences; these include… ...
... Replication are similar but there are some differences; these include… ...
Site-Directed Mutagenesis Analysis of Pils, a Type IVB Pilin
... PilS is a Type IVb pilin and forms the pili of Salmonella typhi which is a major adhesion factor for this pathogen to enter the human gastrointestinal epithelial cells and cause typhoid fever in infected patients. The aim of this project is to investigate the impact of mutation of two positively cha ...
... PilS is a Type IVb pilin and forms the pili of Salmonella typhi which is a major adhesion factor for this pathogen to enter the human gastrointestinal epithelial cells and cause typhoid fever in infected patients. The aim of this project is to investigate the impact of mutation of two positively cha ...
7. Nucleic acids
... a deoxyribose sugar and three phosphate groups. They are sometimes referred to as dATP, dCTP, dGTP and dTTP. • Before a new strand of DNA can be formed, it is necessary to start with an RNA primer. The RNA primer is a few RNA nucleotides which bind to the old DNA strand (hydrogen bonding between th ...
... a deoxyribose sugar and three phosphate groups. They are sometimes referred to as dATP, dCTP, dGTP and dTTP. • Before a new strand of DNA can be formed, it is necessary to start with an RNA primer. The RNA primer is a few RNA nucleotides which bind to the old DNA strand (hydrogen bonding between th ...
DNA - Warren County Schools
... *DNA molecules are incredibly long. If all of the DNA from all of your cells was stretched out into a single thread, it would extend to the moon and back ...
... *DNA molecules are incredibly long. If all of the DNA from all of your cells was stretched out into a single thread, it would extend to the moon and back ...
CHROMOSOME FUSION
... those in our chromosome 2. Do you see the white band located there, labeled 2q13? B. INTERESTING QUESTION: Why does our chromosome #2 appear to be so very similar to the two shorter chromosomes found in apes (chimpanzees, gorillas, and orangutans)? C. SOME POSSIBLE EXPLANATIONS (Building a Working H ...
... those in our chromosome 2. Do you see the white band located there, labeled 2q13? B. INTERESTING QUESTION: Why does our chromosome #2 appear to be so very similar to the two shorter chromosomes found in apes (chimpanzees, gorillas, and orangutans)? C. SOME POSSIBLE EXPLANATIONS (Building a Working H ...
Creating 3-Dimensional Graph Structures with DNA
... NATASA JONOSKA, STEPHEN A. KARL, AND MASAHICO SAITO ...
... NATASA JONOSKA, STEPHEN A. KARL, AND MASAHICO SAITO ...
Gel electrophoresis of restriction digest
... Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5 to 25Kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) ...
... Agarose gel electrophoresis is a simple and highly effective method for separating, identifying, and purifying 0.5 to 25Kb DNA fragments. The protocol can be divided into three stages: (1) a gel is prepared with an agarose concentration appropriate for the size of DNA fragments to be separated; (2) ...
zChap01_140901 - Online Open Genetics
... characteristics. It includes the study of genes, themselves, how they function, interact, and produce the visible and measurable characteristics we see in individuals and populations of species as they change from one generation to the next, over time, and in different environments. Heredity – Human ...
... characteristics. It includes the study of genes, themselves, how they function, interact, and produce the visible and measurable characteristics we see in individuals and populations of species as they change from one generation to the next, over time, and in different environments. Heredity – Human ...
Structural characterization and DNA-binding properties of
... [2] G. Zuber, J.C. Quada and S.M. Hecht, Sequence selective cleavage of a DNA octanucleotide by chlorinated bithiazoles and bleomycins, J. Am. Chem. Soc. 120 (1998), 9368–9369. [3] I. Haq, B. Lincoln, B.Z. Choedhry and J.B. Charires, Intercalation of A- and A- [Ru(phen)zDPpZ]2 with DNA: A calorimeri ...
... [2] G. Zuber, J.C. Quada and S.M. Hecht, Sequence selective cleavage of a DNA octanucleotide by chlorinated bithiazoles and bleomycins, J. Am. Chem. Soc. 120 (1998), 9368–9369. [3] I. Haq, B. Lincoln, B.Z. Choedhry and J.B. Charires, Intercalation of A- and A- [Ru(phen)zDPpZ]2 with DNA: A calorimeri ...
Restriction of M13 DNA by the restriction enzyme TaqI
... You should get a pop-up window containing the coordinates of each TaqI site and the distances between each site, i.e. the sizes of the fragments produced by digesting M13 with TaqI. SQR2. How many TaqI sites are there? How many fragments are produced? Why that number and not one more? SQR3. Compare ...
... You should get a pop-up window containing the coordinates of each TaqI site and the distances between each site, i.e. the sizes of the fragments produced by digesting M13 with TaqI. SQR2. How many TaqI sites are there? How many fragments are produced? Why that number and not one more? SQR3. Compare ...
On joint maximum-likelihood estimation of PCR efficiency and initial
... of infectious and hereditary diseases, genetic fingerprinting, etc. Typically, a given sample contains only a small amount of the target which needs to be detected and quantified. PCR replicates the target using two primers which serve as initiation sites for a DNA polymerase enzyme. The reaction is ...
... of infectious and hereditary diseases, genetic fingerprinting, etc. Typically, a given sample contains only a small amount of the target which needs to be detected and quantified. PCR replicates the target using two primers which serve as initiation sites for a DNA polymerase enzyme. The reaction is ...
Potassium hydroxide - Sigma
... hydroxide is for applications which require tight control of elemental content. Potassium hydroxide (KOH) is a caustic reagent that is widely used to neutralize acids and prepare potassium salts of reagents. It is used in a variety of large-scale applications, such as the manufacture of soap, the me ...
... hydroxide is for applications which require tight control of elemental content. Potassium hydroxide (KOH) is a caustic reagent that is widely used to neutralize acids and prepare potassium salts of reagents. It is used in a variety of large-scale applications, such as the manufacture of soap, the me ...
Restriction Endonuclease Troubleshooting Guide - IMBB
... strength and pH, Mg2+ concentration. Sometimes the presence of BSA in the reaction mix has the crucial influence on the activity of enzyme, because it stabilizes the enzyme, binds some impurities, prevents the enzyme sorption on the test tube surface. Reaction conditions for each endonuclease are in ...
... strength and pH, Mg2+ concentration. Sometimes the presence of BSA in the reaction mix has the crucial influence on the activity of enzyme, because it stabilizes the enzyme, binds some impurities, prevents the enzyme sorption on the test tube surface. Reaction conditions for each endonuclease are in ...
DNA - York University
... At the place where the DNA is open, enzymes cause a backbone of ribose and phospate to form and attract to it the purines and pyrimidines that are the complements of the exposed bases on the DNA. This forms a piece of RNA (which is single stranded). The piece of RNA that has formed and copied the se ...
... At the place where the DNA is open, enzymes cause a backbone of ribose and phospate to form and attract to it the purines and pyrimidines that are the complements of the exposed bases on the DNA. This forms a piece of RNA (which is single stranded). The piece of RNA that has formed and copied the se ...
Identification of the target DNA sequence and characterization of
... HlyU Vc binds to an imperfect palindrome about 164 bp upstream of hlyA transcription start site As Williams and Manning showed a 710-bp DNA sequence upstream of hlyA gene in conjunction with HlyU Vc increases HlyA production (16), we scanned the region upstream of the hlyA gene for the precise delin ...
... HlyU Vc binds to an imperfect palindrome about 164 bp upstream of hlyA transcription start site As Williams and Manning showed a 710-bp DNA sequence upstream of hlyA gene in conjunction with HlyU Vc increases HlyA production (16), we scanned the region upstream of the hlyA gene for the precise delin ...
Document
... Steps in Translation – The polypeptide chain continues to grow until the ribosome reaches a “stop” codon on the mRNA molecule. – When the ribosome reaches a stop codon, it releases both the newly formed polypeptide and the mRNA molecule, completing the process of ...
... Steps in Translation – The polypeptide chain continues to grow until the ribosome reaches a “stop” codon on the mRNA molecule. – When the ribosome reaches a stop codon, it releases both the newly formed polypeptide and the mRNA molecule, completing the process of ...
IOSR Journal of Computer Engineering (IOSR-JCE)
... The steps for decryption are reverse of the encryption algorithm. 1. Initially, the encrypted matrix is XORed with the last key. 2. Then the matrix undergoes eight rounds of DNA module with the round keys. 3. The matrix so obtained is XORed with the first key to get the block of plaintext. 4. This b ...
... The steps for decryption are reverse of the encryption algorithm. 1. Initially, the encrypted matrix is XORed with the last key. 2. Then the matrix undergoes eight rounds of DNA module with the round keys. 3. The matrix so obtained is XORed with the first key to get the block of plaintext. 4. This b ...
Nucleic Acids Nucleotides
... At the place where the DNA is open, enzymes cause a backbone of ribose and phospate to form and attract to it the purines and pyrimidines that are the complements of the exposed bases on the DNA. This forms a piece of RNA (which is single stranded). The piece of RNA that has formed and copied the se ...
... At the place where the DNA is open, enzymes cause a backbone of ribose and phospate to form and attract to it the purines and pyrimidines that are the complements of the exposed bases on the DNA. This forms a piece of RNA (which is single stranded). The piece of RNA that has formed and copied the se ...
Bioinformatics (Warm Up + Cracking the Genetic Code)
... • Garmov - Diamond Code • Crick - Non-Overlapping Commafree Code ...
... • Garmov - Diamond Code • Crick - Non-Overlapping Commafree Code ...
striking similarities in diverse telomerase proteins revealed by
... in [36]. The performance of protein-DNA interface predictor trained on the DB208 dataset (DNABindR, http://cild.iastate.edu/DNABindR) and estimated using 10-fold sequence-based cross-validation, is comparable to that of the previously published protein-DNA interface predictor, which was trained on t ...
... in [36]. The performance of protein-DNA interface predictor trained on the DB208 dataset (DNABindR, http://cild.iastate.edu/DNABindR) and estimated using 10-fold sequence-based cross-validation, is comparable to that of the previously published protein-DNA interface predictor, which was trained on t ...
Curriculum vitae
... Email: [email protected] Mobile Phone No: 0030-6977852035 FAX: 0030-2551029145 ...
... Email: [email protected] Mobile Phone No: 0030-6977852035 FAX: 0030-2551029145 ...
Oligonucleotide-directed mutagenesis of gene IX of bacteriophage
... mutants a purified phage stock was prepared and the mutant viral DNA was isolated by phenol/chloroform extraction. A preliminary screening for the gene IX amber mutant character was performed by marker rescue experiments with purified M13 restriction fragments (25). Surprisingly, only the viral DNA ...
... mutants a purified phage stock was prepared and the mutant viral DNA was isolated by phenol/chloroform extraction. A preliminary screening for the gene IX amber mutant character was performed by marker rescue experiments with purified M13 restriction fragments (25). Surprisingly, only the viral DNA ...
DNA nanotechnology
DNA nanotechnology is the design and manufacture of artificial nucleic acid structures for technological uses. In this field, nucleic acids are used as non-biological engineering materials for nanotechnology rather than as the carriers of genetic information in living cells. Researchers in the field have created static structures such as two- and three-dimensional crystal lattices, nanotubes, polyhedra, and arbitrary shapes, as well as functional devices such as molecular machines and DNA computers. The field is beginning to be used as a tool to solve basic science problems in structural biology and biophysics, including applications in crystallography and spectroscopy for protein structure determination. Potential applications in molecular scale electronics and nanomedicine are also being investigated.The conceptual foundation for DNA nanotechnology was first laid out by Nadrian Seeman in the early 1980s, and the field began to attract widespread interest in the mid-2000s. This use of nucleic acids is enabled by their strict base pairing rules, which cause only portions of strands with complementary base sequences to bind together to form strong, rigid double helix structures. This allows for the rational design of base sequences that will selectively assemble to form complex target structures with precisely controlled nanoscale features. A number of assembly methods are used to make these structures, including tile-based structures that assemble from smaller structures, folding structures using the DNA origami method, and dynamically reconfigurable structures using strand displacement techniques. While the field's name specifically references DNA, the same principles have been used with other types of nucleic acids as well, leading to the occasional use of the alternative name nucleic acid nanotechnology.