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Polymerase Chain Reaction (PCR) Techniques
Polymerase Chain Reaction (PCR) Techniques

... PCR is a technique used to amplify a single, or a few copies of, a piece of DNA (or in some cases RNA), generating thousands to millions of copies of the particular DNA sequence. PCR relies on thermal cycling, which consists of repeated cycles of heating and cooling the reaction involving DNA. Prime ...
Chapter 9 DNA: The Genetic Material Read 192
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... • DNA helicase unwinds the DNA and breaks the hydrogen bonds that hold the 2 strands of DNA together. • DNA polymerase forms the new halves of DNA by putting the correct nucleotides into position. It also proofreads the new DNA built for any errors. • This process occurs once in a cell’s lifetime- r ...
Bio 309F
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... -27. A woman was found to have a mosaic disorder called anhidrotic ectodermal dysplasia where patches of skin have either sweat glands or no sweat glands. How would one account for this? A. X-inactivation B. autosomal recessive trait C. autosomal dominant trait D. A, B,and C could account for the m ...
Join us in downtown Chicago, July 27-29, at the
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... If you have already taken DNAcreator v1 or v2 training, this session will certify you to submit DNAapps created using DNAcreator v3 for validation. Brand new developers are welcome to attend but will need to complete DNAcreator v1 training at a later date to be fully certified. Some programming back ...
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Biol-1406_Ch9Notes.ppt

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... • RNA ______________________________attaches to a special place (certain base sequence called ____________________________) on the DNA molecule and moves along the strand, unwinding and separating the strands. • The RNA polymerase then begins reading and copying the DNA as it goes along. “zips up” a ...
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... b. Another enzyme, called ___________________________, adds new bases to the new RNA primer. It always reads the strand from _______ to _____ and lays down the new strand from _____ to _______. This occurs continuously in the direction following Helicase opening up the replication fork. For this rea ...
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... Steps in the process of Replication Enzyme Helicase unwinds the DNA helix (1A) A Y-shaped Replication Fork results (1B) Single stranded DNA binding proteins prevent the strands from recombining (1C) Topoisomerase removes any twists or knots that form (1D) RNA Primase initiates DNA replication at spe ...
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... Gene sequences code for protein sequences via a symbolic code, the genetic code. This code is used nearly universally by living organisms; it is one of the most ancient shared characteristics of living things.  The “words” of the genetic code are nucleotide triplets called codons. Each codon codes ...
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... The double helical structure of DNA also explains how DNA replicates or copies itself. Each strand of DNA has all the information needed to reconstruct the other half by the rules of base pairing. ...
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Chapter 12: Nucleotides and Nucleic Acids
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... determined that all the basic rules of protein and nucleic acid structure and synthesis are the same in Oz as they are on earth, with only two apparent exceptions. First, in Oz, only 12 different amino acids could be detected in protein samples (Gly, Pro, Leu, Lys, Arg, Phe, Tyr, Glu, Ser, Cys, Gln, ...
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14.1 Structure of Ribonucleic Acid (RNA)

Ch 8-11 Review
Ch 8-11 Review

... genotype and phenotype of the offspring be? 13. What characteristics can make genetic disorders more likely to be passed from one generation to the next? (at least 3) 14. Describe the process of DNA replication. What is meant by semiconservative replication? How are continuous synthesis and disconti ...
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DNA polymerase



The DNA polymerases are enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase “reads” the existing DNA strands to create two new strands that match the existing ones.Every time a cell divides, DNA polymerase is required to help duplicate the cell’s DNA, so that a copy of the original DNA molecule can be passed to each of the daughter cells. In this way, genetic information is transmitted from generation to generation.Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightly woven form. This opens up or “unzips” the double-stranded DNA to give two single strands of DNA that can be used as templates for replication.
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