![Development of an internally controlled real-time PCR](http://s1.studyres.com/store/data/001773381_1-1d4aa45a5ef06fcd5fc1caad582861e6-300x300.png)
Development of an internally controlled real-time PCR
... designed during the present study also amplified and detected these other three species, but because of sequence homology in the ompA gene, it was not possible to design primers that could distinguish these four species in a LightCycler assay. However, for clinical purposes, this is not important, s ...
... designed during the present study also amplified and detected these other three species, but because of sequence homology in the ompA gene, it was not possible to design primers that could distinguish these four species in a LightCycler assay. However, for clinical purposes, this is not important, s ...
Resolvase OsGEN1 Mediates DNA Repair by
... between sister or homologous chromatids that form DNA joint molecules (JMs; Mimitou and Symington, 2009). Most JMs are repaired by the synthesis-dependent strand annealing pathway (Andersen and Sekelsky, 2010; Sarbajna et al., 2014). The remaining JMs may be ligated to form Holliday junctions (HJs), ...
... between sister or homologous chromatids that form DNA joint molecules (JMs; Mimitou and Symington, 2009). Most JMs are repaired by the synthesis-dependent strand annealing pathway (Andersen and Sekelsky, 2010; Sarbajna et al., 2014). The remaining JMs may be ligated to form Holliday junctions (HJs), ...
1 - life.illinois.edu
... DNA). The lambda phage grown on the E. coli K (P1) host is modified for both K and P1 sites so they are resistant to both K and P1 restriction systems. b. In a second experiment, they labeled lambda DNA of phage growing in E. coli K (P1) with 32P so that the newly synthesized DNA in the phage was ra ...
... DNA). The lambda phage grown on the E. coli K (P1) host is modified for both K and P1 sites so they are resistant to both K and P1 restriction systems. b. In a second experiment, they labeled lambda DNA of phage growing in E. coli K (P1) with 32P so that the newly synthesized DNA in the phage was ra ...
Full-Text PDF
... a 15-residue-long linker, and it contains two conserved zinc-binding modules, Zn1 and Zn2 (Figure 1), where each zinc is coordinated by four cysteines [19,24]. Zn2 and the helical region between the two zinc-binding modules provide the extended interaction interface (~4000 Å2 ) with p70, while the s ...
... a 15-residue-long linker, and it contains two conserved zinc-binding modules, Zn1 and Zn2 (Figure 1), where each zinc is coordinated by four cysteines [19,24]. Zn2 and the helical region between the two zinc-binding modules provide the extended interaction interface (~4000 Å2 ) with p70, while the s ...
ucscDatabase
... • Most of the information in a GenBank flat file record ends up in the genome database. • The mrna table contains an entry for every mRNA, EST, and RefSeq. • The mrna table itself just contains the GenBank accession, and id’s that link into other tables. ...
... • Most of the information in a GenBank flat file record ends up in the genome database. • The mrna table contains an entry for every mRNA, EST, and RefSeq. • The mrna table itself just contains the GenBank accession, and id’s that link into other tables. ...
When epigenetics meets alternative splicing: the roles of DNA
... structures evolved: one located in high GC content regions and the other in low GC content regions. During mammalian and avian evolution GC-rich regions underwent a GC increase that resulted in even higher GC values. Genes located in these high GC content regions have a much less pronounced differen ...
... structures evolved: one located in high GC content regions and the other in low GC content regions. During mammalian and avian evolution GC-rich regions underwent a GC increase that resulted in even higher GC values. Genes located in these high GC content regions have a much less pronounced differen ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... among isolates that are members of the same anastomosis group. Isolates of AG-3, AG-5, AG-7, and AG-BI hybridized with other isolates within their respective groups at a rate of 91% or higher (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of di ...
... among isolates that are members of the same anastomosis group. Isolates of AG-3, AG-5, AG-7, and AG-BI hybridized with other isolates within their respective groups at a rate of 91% or higher (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of di ...
GeNeViSTA Coffin Siris Syndrome: A Disorder of SWI/SNF Pathway
... Two mechanisms of chromosome remodeling by SWI/SNF have been proposed. The rst model involves a unidirectional diffusion of a twist defect in the nucleosomal DNA that starts at the DNA entry site of the nucleosome and results in a corkscrew-like propagation of DNA on the histone octamer surface. The ...
... Two mechanisms of chromosome remodeling by SWI/SNF have been proposed. The rst model involves a unidirectional diffusion of a twist defect in the nucleosomal DNA that starts at the DNA entry site of the nucleosome and results in a corkscrew-like propagation of DNA on the histone octamer surface. The ...
The past, present and future of plant breeding
... occurred (and they still do now). These can occur ...
... occurred (and they still do now). These can occur ...
A multiplex PCR assay for the identification of animal species
... possible to apply the assay to samples whose DNA is highly degraded by heat following the treatment indicated by the EU Law for thermal processing of animal ingredients (134.4 – 141.9 8C and 3.03 – 4.03 bar for 24 min). This degradation has caused some drawbacks in PCR application as observed by Bot ...
... possible to apply the assay to samples whose DNA is highly degraded by heat following the treatment indicated by the EU Law for thermal processing of animal ingredients (134.4 – 141.9 8C and 3.03 – 4.03 bar for 24 min). This degradation has caused some drawbacks in PCR application as observed by Bot ...
letters
... with DNA methyltransferases (Fig. 4c). It is well established that in various organisms H3K9 methylation can be a prerequisite to DNA methylation17–19, which in mammals involves the SUV39H enzymes and also probably the HP1 (heterochromatin protein 1) adaptor13,19. It should be noted, however, that t ...
... with DNA methyltransferases (Fig. 4c). It is well established that in various organisms H3K9 methylation can be a prerequisite to DNA methylation17–19, which in mammals involves the SUV39H enzymes and also probably the HP1 (heterochromatin protein 1) adaptor13,19. It should be noted, however, that t ...
Noninvasive prenatal diagnosis of fetal chromosomal aneuploidies
... fetal chromosomal aneuploidy, using trisomy 18 as a model system (see later sections) (20 ). Since the development of the SERPINB5 marker, many other fetal epigenetic markers suitable for detection in maternal plasma have been described, including the RASSF1A gene on chromosome 3 (21, 22 ) and numer ...
... fetal chromosomal aneuploidy, using trisomy 18 as a model system (see later sections) (20 ). Since the development of the SERPINB5 marker, many other fetal epigenetic markers suitable for detection in maternal plasma have been described, including the RASSF1A gene on chromosome 3 (21, 22 ) and numer ...
Detection of Agrobacterium vitis by polymerase chain reaction in
... sap sample was streaked onto tartrate plates (AB minimal medium with 0.5 % (w/v) L(+)tartrate and 2.5 mg·l -1 bromothymolblue) and incubated at 27 °C for 7 d. Then a half loop of colonies resembling Agrobacterium was suspended in 0.9 ml of sterile distilled water, supplemented with 0.1 ml 10 % (v/v) ...
... sap sample was streaked onto tartrate plates (AB minimal medium with 0.5 % (w/v) L(+)tartrate and 2.5 mg·l -1 bromothymolblue) and incubated at 27 °C for 7 d. Then a half loop of colonies resembling Agrobacterium was suspended in 0.9 ml of sterile distilled water, supplemented with 0.1 ml 10 % (v/v) ...
forensic odontology
... b). Class features: Incisorrectangular Caninestriangular or rectangular Premolars and molars spherical or point shaped c). Individual features: Class features intern have characteristics such as fractures rotation etc. • Site of Bite Marks Females on breast, legs( inner part of thigh)-sexual ass ...
... b). Class features: Incisorrectangular Caninestriangular or rectangular Premolars and molars spherical or point shaped c). Individual features: Class features intern have characteristics such as fractures rotation etc. • Site of Bite Marks Females on breast, legs( inner part of thigh)-sexual ass ...
Implications of DNA replication for eukaryotic gene expression
... slow. In vitro, the process of assembling a complete transcription complex takes several minutes. This understanding of transcription complex assembly follows from experiments using fractionated proteins and naked DNA as a substrate. In vivo the template for transcription is chromatin, the complex o ...
... slow. In vitro, the process of assembling a complete transcription complex takes several minutes. This understanding of transcription complex assembly follows from experiments using fractionated proteins and naked DNA as a substrate. In vivo the template for transcription is chromatin, the complex o ...
Using the Wolbachia Bacterial Symbiont to Teach Inquiry
... bacterium Wolbachia and invertebrates. This endeavor, called “Discover the Microbes In addition to insects, Wolbachia are also known to infect filarial Within! The Wolbachia Project,” has the following goals: (1) involve students in grade 7 through college in a nationwide effort to collect new genet ...
... bacterium Wolbachia and invertebrates. This endeavor, called “Discover the Microbes In addition to insects, Wolbachia are also known to infect filarial Within! The Wolbachia Project,” has the following goals: (1) involve students in grade 7 through college in a nationwide effort to collect new genet ...
Recombinant DNA Technology I Restriction Enzymes
... algorithm and search for high scoring alignments. – The alignment scores are then statistically evaluated – one example is the E value discussed previously. – BLAST is actually a family of programs. ...
... algorithm and search for high scoring alignments. – The alignment scores are then statistically evaluated – one example is the E value discussed previously. – BLAST is actually a family of programs. ...
J. Biol. Chem.
... TPT versus ara-C plus TPT lead us to ask whether cDDP adducts would also act to poison Top1 or induce distinct DNA lesions that trigger aberrant repair by the HR pathways (20). cDDP may interact with nucleophiles in many cellular components, including DNA, RNA, and proteins. However, it is generally ...
... TPT versus ara-C plus TPT lead us to ask whether cDDP adducts would also act to poison Top1 or induce distinct DNA lesions that trigger aberrant repair by the HR pathways (20). cDDP may interact with nucleophiles in many cellular components, including DNA, RNA, and proteins. However, it is generally ...
De novo DNA cytosine methyltransferase activities in
... Wild-type and mutant ES cells were cultured individually for two passages and then mixed together at about 1:1 ratio and cultured for 2 days. Cells were then trypsinized and divided into three fractions. One fraction was used for DNA isolation (i.e. d0 in Fig. 8, which represents the inital ratio of ...
... Wild-type and mutant ES cells were cultured individually for two passages and then mixed together at about 1:1 ratio and cultured for 2 days. Cells were then trypsinized and divided into three fractions. One fraction was used for DNA isolation (i.e. d0 in Fig. 8, which represents the inital ratio of ...
RAPD markers for identifying oil palm (Elaeis guineensis Jacq
... identification of oil palm varieties at vegetative phase by comparing the activity of marker enzymes of lignin synthesis reveal the possibility of distinguishing the varieties biochemically under ideal conditions5, 6. Advent of molecular marker techniques has provided a different and sensitive appro ...
... identification of oil palm varieties at vegetative phase by comparing the activity of marker enzymes of lignin synthesis reveal the possibility of distinguishing the varieties biochemically under ideal conditions5, 6. Advent of molecular marker techniques has provided a different and sensitive appro ...
Rosalind Franklin and the Double Helix
... showed that the bases of the A form are tilted and curved slightly, and that 11 pairs of bases are compacted within a repeat distance of 28.1 Å. In her section of the 1952 King's Medical Research Council (MRC) report, Franklin gave quantitative measurements for the interphosphate distances and discu ...
... showed that the bases of the A form are tilted and curved slightly, and that 11 pairs of bases are compacted within a repeat distance of 28.1 Å. In her section of the 1952 King's Medical Research Council (MRC) report, Franklin gave quantitative measurements for the interphosphate distances and discu ...
Complete Laboratory PDF
... polymorphisms that are detected by molecular methods. In Arabidopsis, molecular markers exploit the natural differences between distinct ecotypes, such as the widely used Landsberg (Ler) and Columbia (Col), which differ about 1% at the DNA level. This laboratory uses linkage to map ago1 (argonaute), ...
... polymorphisms that are detected by molecular methods. In Arabidopsis, molecular markers exploit the natural differences between distinct ecotypes, such as the widely used Landsberg (Ler) and Columbia (Col), which differ about 1% at the DNA level. This laboratory uses linkage to map ago1 (argonaute), ...
Natural Transfer of Conjugative Transposon Tn916 between Gram
... gram-negative eubacteria, with subsequent expression in the new host. Although we did not make any attempt to determine the nature of the transfer process (transformation versus conjugation), there are indications that favor a conjugationlike mechanism. First, C. acetobutylicum has been reported to ...
... gram-negative eubacteria, with subsequent expression in the new host. Although we did not make any attempt to determine the nature of the transfer process (transformation versus conjugation), there are indications that favor a conjugationlike mechanism. First, C. acetobutylicum has been reported to ...
Can Jury Trial Innovations Improve Juror Understanding of DNA
... the mtDNA profiles of hair from the sweatshirt and the samples combed from the defendant’s head at the time of his police interview were an exact match. He commented that the profile was rare and had not been observed in the Federal Bureau of Investigation’s (FBI’s) mtDNA database of more than 5,000 ...
... the mtDNA profiles of hair from the sweatshirt and the samples combed from the defendant’s head at the time of his police interview were an exact match. He commented that the profile was rare and had not been observed in the Federal Bureau of Investigation’s (FBI’s) mtDNA database of more than 5,000 ...
2. Assessing the probative value of DNA evidence
... the Crown Prosecution Service, the National Policing Improvement Agency (NPIA)5 and the Forensic Science Service,6 as well as academic lawyers, statisticians and forensic scientists. ...
... the Crown Prosecution Service, the National Policing Improvement Agency (NPIA)5 and the Forensic Science Service,6 as well as academic lawyers, statisticians and forensic scientists. ...