Technological applications arising from the interactions of DNA
... In addition, nucleic acids undergo straightforward sequence-dependent hybridization with complementary strands and they capture specific target molecules with high specificities and affinities. This property enables information to be conveniently and specifically programmed into a variety of DNA nan ...
... In addition, nucleic acids undergo straightforward sequence-dependent hybridization with complementary strands and they capture specific target molecules with high specificities and affinities. This property enables information to be conveniently and specifically programmed into a variety of DNA nan ...
Caitlin Davis
... Plasmids are small circular pieces of DNA found in bacteria. They are commonly used in biotechnology as vectors: DNA that can accept, carry, and replicate other pieces of DNA (Thieman and Palladino, 2009). Restriction enzymes are enzymes that recognize segments of DNA, called restriction sites, and ...
... Plasmids are small circular pieces of DNA found in bacteria. They are commonly used in biotechnology as vectors: DNA that can accept, carry, and replicate other pieces of DNA (Thieman and Palladino, 2009). Restriction enzymes are enzymes that recognize segments of DNA, called restriction sites, and ...
chapter9_From DNA to Protein(1
... dose as small as a few grains of salt can kill an adult • Ricin inactivates ribosomes – organelles that assemble amino acids into proteins • Proteins are critical to all life processes, so cells that cannot make them die very quickly ...
... dose as small as a few grains of salt can kill an adult • Ricin inactivates ribosomes – organelles that assemble amino acids into proteins • Proteins are critical to all life processes, so cells that cannot make them die very quickly ...
Chapter 5 Gases - Colorado Mountain College
... dose as small as a few grains of salt can kill an adult • Ricin inactivates ribosomes – organelles that assemble amino acids into proteins • Proteins are critical to all life processes, so cells that cannot make them die very quickly ...
... dose as small as a few grains of salt can kill an adult • Ricin inactivates ribosomes – organelles that assemble amino acids into proteins • Proteins are critical to all life processes, so cells that cannot make them die very quickly ...
pdf file - Collins Lab @ MIT
... mutagenesis and as a significant contributor to the mutational burden experienced by microbes during periods of oxidative stress (e.g. [11–13]). This notion is supported by the existence of several overlapping enzymatic mechanisms employed by bacteria to combat ROS toxicity [14]. ROS are generated ...
... mutagenesis and as a significant contributor to the mutational burden experienced by microbes during periods of oxidative stress (e.g. [11–13]). This notion is supported by the existence of several overlapping enzymatic mechanisms employed by bacteria to combat ROS toxicity [14]. ROS are generated ...
Rapid Purification of DNA with High PCR Efficiency
... mainly caused by various bacteria, leads to reduced milk quality and yield. Quick, sensitive and accurate detection of the causal bacteria is therefore needed to provide efficient control. DNA/ PCR-based protocols for milk bacteria identification have been developed in recent years to replace the mo ...
... mainly caused by various bacteria, leads to reduced milk quality and yield. Quick, sensitive and accurate detection of the causal bacteria is therefore needed to provide efficient control. DNA/ PCR-based protocols for milk bacteria identification have been developed in recent years to replace the mo ...
STRUCTURE AND DIAGNOSTIC APPLICATIONS OF DNA
... • Nucleotide sequence of each DNA strand is the same when each is read in 5’ to 3’ direction; • Restriction enzymes can cut the Phosphodiester bonds on each DNA strand in three different ways; • Several Restriction Enzymes have been isolated from bacteria; they are named according to the bacterial s ...
... • Nucleotide sequence of each DNA strand is the same when each is read in 5’ to 3’ direction; • Restriction enzymes can cut the Phosphodiester bonds on each DNA strand in three different ways; • Several Restriction Enzymes have been isolated from bacteria; they are named according to the bacterial s ...
DNA-based Intrusion Detection System
... Deoxyribonucleic Acid (DNA) is made two strands of complementary pairs of nucleotides. Each strand is made of different sequences of four bases (nucleotides) Adenine, Guanine, Cytosine, and Thymine. DNA has tremendous information storage capacity. For example, only 1 gram of DNA contains as much inf ...
... Deoxyribonucleic Acid (DNA) is made two strands of complementary pairs of nucleotides. Each strand is made of different sequences of four bases (nucleotides) Adenine, Guanine, Cytosine, and Thymine. DNA has tremendous information storage capacity. For example, only 1 gram of DNA contains as much inf ...
FluoCycle II™ SYBR® Master Mix
... than 80% might be not completely denatured during thermal cycling, causing lower amplification yield. G/C-rich sequences are also prone to nonspecific interactions decreasing the amplification efficiency and producing a non-specific signal in SYBR® Green dye assays. For the same reason, primers cont ...
... than 80% might be not completely denatured during thermal cycling, causing lower amplification yield. G/C-rich sequences are also prone to nonspecific interactions decreasing the amplification efficiency and producing a non-specific signal in SYBR® Green dye assays. For the same reason, primers cont ...
Functional analysis of plastid DNA replication origins in tobacco by
... ß Blackwell Publishing Ltd, The Plant Journal, (2002), 32, 175–184 ...
... ß Blackwell Publishing Ltd, The Plant Journal, (2002), 32, 175–184 ...
CtrA mediates a DNA replication checkpoint that prevents cell
... to cell division genes that are required for late stages in division. The ftsQ and ftsA genes, which are required for late stages of cell division, are co-transcribed from promoter PQA at the end of S phase when ftsZ transcription is repressed by CtrA (Sackett et al., 1998). This suggested the possi ...
... to cell division genes that are required for late stages in division. The ftsQ and ftsA genes, which are required for late stages of cell division, are co-transcribed from promoter PQA at the end of S phase when ftsZ transcription is repressed by CtrA (Sackett et al., 1998). This suggested the possi ...
chapter9_Sections 1
... hydrogen atom or hydroxyl group at the 2’ carbon of the sugar (shown in green). Fig. 9.2b, p. 138 ...
... hydrogen atom or hydroxyl group at the 2’ carbon of the sugar (shown in green). Fig. 9.2b, p. 138 ...
Analysis of high molecular weight genomic DNA using the Agilent
... Genomic DNA Quantification The Genomic DNA ScreenTape assay uses the lower maker to quantify the samples. The samples were run as triplicates and the quantification data was collated for each extraction kit. The samples were also quantified using the Qubit dsDNA broad range assay and NanoDrop spectroph ...
... Genomic DNA Quantification The Genomic DNA ScreenTape assay uses the lower maker to quantify the samples. The samples were run as triplicates and the quantification data was collated for each extraction kit. The samples were also quantified using the Qubit dsDNA broad range assay and NanoDrop spectroph ...
Detection of Genetically Modified Organisms in Foods by Protein
... absence of Roundup Ready DNA, no signals were found by using these primers, which indicates the specificity of this method. For maize, identical series of experiments were performed with fully comparable results (data not shown). These results indicate that this PCR analysis is very sensitive and ac ...
... absence of Roundup Ready DNA, no signals were found by using these primers, which indicates the specificity of this method. For maize, identical series of experiments were performed with fully comparable results (data not shown). These results indicate that this PCR analysis is very sensitive and ac ...
DNA Sequencing Handbook
... your primer. Our thermocycling protocol anneals at 50°C and extends at 60°C. If the Tm of your primer is on the low side, please consider redesigning a longer primer. When the Tm is too low, the primer may anneal incorrectly or not at all. A high Tm can be OK if there are not long strings (>3) of Gs ...
... your primer. Our thermocycling protocol anneals at 50°C and extends at 60°C. If the Tm of your primer is on the low side, please consider redesigning a longer primer. When the Tm is too low, the primer may anneal incorrectly or not at all. A high Tm can be OK if there are not long strings (>3) of Gs ...
1 Generating a Synthetic Genome by Whole Genome Assembly
... reaction, similar to a PCR reaction, but without a pair of primers present in excess compared with template (14). At each cycle, DNA is melted and overlapping single strands reanneal. If the 3’-ends of reannealed strands are such that they can be extended using the opposite strand as template, then ...
... reaction, similar to a PCR reaction, but without a pair of primers present in excess compared with template (14). At each cycle, DNA is melted and overlapping single strands reanneal. If the 3’-ends of reannealed strands are such that they can be extended using the opposite strand as template, then ...
Chapter 7: Essentials of Molecular Biology Question: Which strand
... leading strand, ligase, DNA polymerase lagging strand, primer, helicase single-strand binding protein, replication fork, promoter DNA polymerase III, DNA polymerase I, ligase ...
... leading strand, ligase, DNA polymerase lagging strand, primer, helicase single-strand binding protein, replication fork, promoter DNA polymerase III, DNA polymerase I, ligase ...
DNA Metallization Processes and Nanoelectronics
... Concerning the intercalation mechanism of metal complexes between the DNA bases, the Pd or Pt complexes have been the more extensively studied. Indeed, the binding process of Pt(II) complexes to DNA is well investigated in the case of cisplatin (cis-[Pt(NH3)2Cl2]), which is widely used as an antican ...
... Concerning the intercalation mechanism of metal complexes between the DNA bases, the Pd or Pt complexes have been the more extensively studied. Indeed, the binding process of Pt(II) complexes to DNA is well investigated in the case of cisplatin (cis-[Pt(NH3)2Cl2]), which is widely used as an antican ...
DNA PROVIDER bro.indd - the National Center for Victims of Crime
... DNA profiles created for criminal justice purposes contain a certain set of identifiers or characteristics, which are found at specific points—called loci—on the DNA ...
... DNA profiles created for criminal justice purposes contain a certain set of identifiers or characteristics, which are found at specific points—called loci—on the DNA ...
TaqI, 10 U/uL, 3000U - Thermo Fisher Scientific
... Thermo Scientific Tango Buffer is provided to simplify buffer selection for double digests. 98% of Thermo Scientific restriction enzymes are active in a 1X or 2X concentration of Tango™ Buffer. Please refer to www.thermoscientific.com/doubledigest to choose the best buffer for your experiments. 1X T ...
... Thermo Scientific Tango Buffer is provided to simplify buffer selection for double digests. 98% of Thermo Scientific restriction enzymes are active in a 1X or 2X concentration of Tango™ Buffer. Please refer to www.thermoscientific.com/doubledigest to choose the best buffer for your experiments. 1X T ...
The Occurrence of 6-Methylaminopurine in Deoxyribonucleic Acids
... E. coli, Aerobacter aerogenes and Mycobacterium tuberculosis. DNA was prepared from these bacteria by the method of Smith & Wyatt (1951). This method involves extraction with N-KOH and gives a product completely free of ribonucleic acid (RNA) but considerably depolymerized. Polymerized DNA was prepa ...
... E. coli, Aerobacter aerogenes and Mycobacterium tuberculosis. DNA was prepared from these bacteria by the method of Smith & Wyatt (1951). This method involves extraction with N-KOH and gives a product completely free of ribonucleic acid (RNA) but considerably depolymerized. Polymerized DNA was prepa ...
Microbial Genetics
... carry information, how information is expressed, and how genes are replicated Gene: a segment of DNA that encodes a functional product, usually a protein Chromosome: structure containing DNA that physically carries hereditary information; the chromosomes contain the genes Genome: all the genet ...
... carry information, how information is expressed, and how genes are replicated Gene: a segment of DNA that encodes a functional product, usually a protein Chromosome: structure containing DNA that physically carries hereditary information; the chromosomes contain the genes Genome: all the genet ...
DNA Excision Repair Pathways - DNA Replication and Human
... by a DNA polymerase (Fig. 1). In vitro, such repair synthesis is efficiently catalyzed by DNA polymerase-P (Dianov et al. 1992). It has been suggested that because of its limited fidelity, polymerase-P may be utilized in this particular very short patch mode of repair synthesis during BER and not in ...
... by a DNA polymerase (Fig. 1). In vitro, such repair synthesis is efficiently catalyzed by DNA polymerase-P (Dianov et al. 1992). It has been suggested that because of its limited fidelity, polymerase-P may be utilized in this particular very short patch mode of repair synthesis during BER and not in ...
Extreme Radiation Resistance by Interdependent DNA
... this prediction, we have developed an immunofluorescent microscopy method to detect and measure, on single cell level, the newly synthesized DNA in its single- and double-stranded form. The method is based on the specificity of monoclonal anti-5-BrdU antibodies that bind the 5-BrdU moiety in single- ...
... this prediction, we have developed an immunofluorescent microscopy method to detect and measure, on single cell level, the newly synthesized DNA in its single- and double-stranded form. The method is based on the specificity of monoclonal anti-5-BrdU antibodies that bind the 5-BrdU moiety in single- ...
Chapter 9 - People Server at UNCW
... • Used E.coli bacteria infected with a virus that consisted of a protein head surrounding DNA • Grew a batch of virus in a medium containing 35S and 32P • Blender experiments showed that the virus transfers DNA, not protein, into a bacterial cell • Thus, DNA is the genetic material ...
... • Used E.coli bacteria infected with a virus that consisted of a protein head surrounding DNA • Grew a batch of virus in a medium containing 35S and 32P • Blender experiments showed that the virus transfers DNA, not protein, into a bacterial cell • Thus, DNA is the genetic material ...
DNA replication
DNA replication is the process of producing two identical replicas from one original DNA molecule. This biological process occurs in all living organisms and is the basis for biological inheritance. DNA is made up of two strands and each strand of the original DNA molecule serves as a template for the production of the complementary strand, a process referred to as semiconservative replication. Cellular proofreading and error-checking mechanisms ensure near perfect fidelity for DNA replication.In a cell, DNA replication begins at specific locations, or origins of replication, in the genome. Unwinding of DNA at the origin and synthesis of new strands results in replication forks growing bidirectional from the origin. A number of proteins are associated with the replication fork which helps in terms of the initiation and continuation of DNA synthesis. Most prominently, DNA polymerase synthesizes the new DNA by adding complementary nucleotides to the template strand.DNA replication can also be performed in vitro (artificially, outside a cell). DNA polymerases isolated from cells and artificial DNA primers can be used to initiate DNA synthesis at known sequences in a template DNA molecule. The polymerase chain reaction (PCR), a common laboratory technique, cyclically applies such artificial synthesis to amplify a specific target DNA fragment from a pool of DNA.