Birth of fertile bimaternal offspring following
... Figure 1 Birth of fertile bimaternal offspring following intracytoplasmic injection of phESCs. (A) Morphology of a live E13.5 bimaternal embryo. Scale bar, 2 mm. (B) Heat map of the methylation level of ICRs in bimaternal and WT E13.5 fetuses. DMRs with significantly different methylation level betw ...
... Figure 1 Birth of fertile bimaternal offspring following intracytoplasmic injection of phESCs. (A) Morphology of a live E13.5 bimaternal embryo. Scale bar, 2 mm. (B) Heat map of the methylation level of ICRs in bimaternal and WT E13.5 fetuses. DMRs with significantly different methylation level betw ...
Caspary T, Cleary MA, Perlman EJ, Zhang P, Elledge SJ, and Tilghman SM. Genes Dev. 1999 Dec 1;13(23):3115-24. Oppositely imprinted genes p57Kip2 and Igf2 interact in a mouse model for Beckwith-Wiedemann syndrome.
... animals exhibited overgrowth, polydactyly, and polyhydramnious, all symptoms of BWS patients. Together, these animal models support the hypothesis that BWS results from elevated expression of IGF2. The impact of loss-of-function mutations in p57Kip2 has also been examined in mice (Yan et al. 1997; Z ...
... animals exhibited overgrowth, polydactyly, and polyhydramnious, all symptoms of BWS patients. Together, these animal models support the hypothesis that BWS results from elevated expression of IGF2. The impact of loss-of-function mutations in p57Kip2 has also been examined in mice (Yan et al. 1997; Z ...
Caspary T, Cleary MA, Baker CC, Guan XJ, Tilghman SM. Mol Cell Biol. 1998 Jun;18(6):3466-74. Multiple mechanisms of imprinting on distal mouse chromosome 7.
... been shown for approximately 20 autosomal genes in mice and humans (4). A fundamental question about imprinting involves the mechanism used for distinguishing the maternal and paternal alleles of a gene. The leading candidate is DNA methylation that is established in different patterns in the male a ...
... been shown for approximately 20 autosomal genes in mice and humans (4). A fundamental question about imprinting involves the mechanism used for distinguishing the maternal and paternal alleles of a gene. The leading candidate is DNA methylation that is established in different patterns in the male a ...
PDF
... Second, H19 was found to be associated with polysomes in different human and mouse cell lines (Li et al., 1998; Milligan et al., 2000). Finally, after transfection of human DiHepG2 cells, H19 was suggested to participate in IGF2 protein repression, in part through transcriptional regulation (Wilkin ...
... Second, H19 was found to be associated with polysomes in different human and mouse cell lines (Li et al., 1998; Milligan et al., 2000). Finally, after transfection of human DiHepG2 cells, H19 was suggested to participate in IGF2 protein repression, in part through transcriptional regulation (Wilkin ...
LECTURE 13: EPIGENETICS – IMPRINTING Reading: Ch. 18, p
... ------------------------------------------------------------------------------------------------------------------In the 1980’s, scientists attempted to create gynogenetic or androgenetic diploids in mammals by putting either two female pronuclei or two male pronuclei into mouse eggs and then transf ...
... ------------------------------------------------------------------------------------------------------------------In the 1980’s, scientists attempted to create gynogenetic or androgenetic diploids in mammals by putting either two female pronuclei or two male pronuclei into mouse eggs and then transf ...
Novel Imprinted DLK1/GTL2 Domain on Human Chromosome 14
... monoallelically expressed in 11 tissues from five different human conceptuses. The expressed allele was determined to be of maternal origin by genotyping matching maternal decidua tissue (Fig. 1B). Using an alternative experimental approach, Miyoshi et al. (2000) recently identified a maternally exp ...
... monoallelically expressed in 11 tissues from five different human conceptuses. The expressed allele was determined to be of maternal origin by genotyping matching maternal decidua tissue (Fig. 1B). Using an alternative experimental approach, Miyoshi et al. (2000) recently identified a maternally exp ...
russell-silver syndrome
... expressed (turned on) in both the paternally and maternally inherited gene copies. Imprinted genes are different in that they are expressed (turned on) in a parent of origin specific manner. H19 works to suppress or hold back growth. Usually, the maternal copy of H19 is expressed (on) and the patern ...
... expressed (turned on) in both the paternally and maternally inherited gene copies. Imprinted genes are different in that they are expressed (turned on) in a parent of origin specific manner. H19 works to suppress or hold back growth. Usually, the maternal copy of H19 is expressed (on) and the patern ...
Document
... this regulation can cause human disease (10). - CDKN1C and IGF2 are both expressed from the same chromosomal region on chromosome 11. They are imprinted, with opposing parent of origin expression. IGF2 is a growth factor, while CDKN1C is a cell cycle inhibitor. Imprinting of each regulates gene dosa ...
... this regulation can cause human disease (10). - CDKN1C and IGF2 are both expressed from the same chromosomal region on chromosome 11. They are imprinted, with opposing parent of origin expression. IGF2 is a growth factor, while CDKN1C is a cell cycle inhibitor. Imprinting of each regulates gene dosa ...
Methylation of an upstream Alu sequence on the Imprinted H19
... Genomic imprinting involves “marking” parental alleles as either maternal or paternal. Such imprints are established during gamete production and involve differential DNA methylation. Unfortunately, however, the exact imprinting mechanism is unknown. DNA methylation is aided by the enzyme DNA methyl ...
... Genomic imprinting involves “marking” parental alleles as either maternal or paternal. Such imprints are established during gamete production and involve differential DNA methylation. Unfortunately, however, the exact imprinting mechanism is unknown. DNA methylation is aided by the enzyme DNA methyl ...
Beckwith-Wiedemann and Russel
... it not only detects DNA methylation abnormalities (epimutations), similar to Southern blot and quantitative methylation sensitive PCR, but it will also detect copy number variations (CNVs; deletions and duplications) of the 11p15 region. The presence of a CNV can increase the recurrence risk up to 5 ...
... it not only detects DNA methylation abnormalities (epimutations), similar to Southern blot and quantitative methylation sensitive PCR, but it will also detect copy number variations (CNVs; deletions and duplications) of the 11p15 region. The presence of a CNV can increase the recurrence risk up to 5 ...
Imprinting
... Is the imprint erased during embryogenesis? • Evidence in support – Biallelic expression of imprinted genes occurs in primordial germ cells – Igf2r imprinting control region (ICR) is methylated in E8 embryos, but unmethylated E12.5 embryos – Dnmt1 is at high levels during Igf2r maternal imprint ...
... Is the imprint erased during embryogenesis? • Evidence in support – Biallelic expression of imprinted genes occurs in primordial germ cells – Igf2r imprinting control region (ICR) is methylated in E8 embryos, but unmethylated E12.5 embryos – Dnmt1 is at high levels during Igf2r maternal imprint ...