SPA1: A New Genetic Locus Involved in Phytochrome A
... is the isolation of mutants defective in normal light signaling (reviewed in Deng, 1994; Millar et al., 1994; McNellis and Deng, 1995; Quail et al., 1995; Chory et al., 1996; von Arnim and Deng, 1996). Several types of screens for mutants have been used. One approach focused on isolating mutants tha ...
... is the isolation of mutants defective in normal light signaling (reviewed in Deng, 1994; Millar et al., 1994; McNellis and Deng, 1995; Quail et al., 1995; Chory et al., 1996; von Arnim and Deng, 1996). Several types of screens for mutants have been used. One approach focused on isolating mutants tha ...
Decreased energy metabolism extends lifespan in
... or behavioral mutant phenotype were transferred onto individual small plates. Strains issued from those F2 animals that produced an entire brood of slow growing, but anatomically wildtype, worms that also had a slow defecation cycle were analyzed further. To determine further the heritability of the ...
... or behavioral mutant phenotype were transferred onto individual small plates. Strains issued from those F2 animals that produced an entire brood of slow growing, but anatomically wildtype, worms that also had a slow defecation cycle were analyzed further. To determine further the heritability of the ...
Two Classes of sir3 Mutants Enhance the sir1
... in previously identified silencing genes SIR2, SIR3, SIR4, NAT1, and ARD1 (Reifsnyder 1996). Twenty-nine mutants in six complementation groups were uncovered. These included 1 allele of sas2 (Reifsnyder et al. 1996), 5 alleles of sir2 (S. Garcia and L. Pillus, unpublished results), 13 alleles of sir ...
... in previously identified silencing genes SIR2, SIR3, SIR4, NAT1, and ARD1 (Reifsnyder 1996). Twenty-nine mutants in six complementation groups were uncovered. These included 1 allele of sas2 (Reifsnyder et al. 1996), 5 alleles of sir2 (S. Garcia and L. Pillus, unpublished results), 13 alleles of sir ...
Functional Interactions of Genes Mediating Convergent Extension
... expression of shh, twhh, Indian hedgehog (Ihh), or a dominant negative form of protein kinase A leads to the reduction of pax6 expression and to the suppression of retina and pigment epithelium. This is accompanied by increased pax2 expression and hypertrophy of the optic stalks in both wild-type an ...
... expression of shh, twhh, Indian hedgehog (Ihh), or a dominant negative form of protein kinase A leads to the reduction of pax6 expression and to the suppression of retina and pigment epithelium. This is accompanied by increased pax2 expression and hypertrophy of the optic stalks in both wild-type an ...
Analysis of large and small colony L5178Y tk−/− mouse lymphoma
... observed in the many mutants analyzed, suggesting that loss of the entire 77t7b allele is not uncommon in tkr1' mutants. The molecular distinction between large and small colony mutants was not clear, since 71fc7b appeared to be completely lost in many mutants in both size classes. Cancer is a genet ...
... observed in the many mutants analyzed, suggesting that loss of the entire 77t7b allele is not uncommon in tkr1' mutants. The molecular distinction between large and small colony mutants was not clear, since 71fc7b appeared to be completely lost in many mutants in both size classes. Cancer is a genet ...
Novel p53 mutants selected in BRCA
... transfection assay of p53-dependent growth arrest in Saos-2 (p537/7) cells. Of the 13 mutants tested, only four (163N, 168Y, 234C and 248W) were completely negative for growth arrest whereas the remaining nine mutants exhibited reproducible growth suppressor activity (Figure 2). Mutants with wild-ty ...
... transfection assay of p53-dependent growth arrest in Saos-2 (p537/7) cells. Of the 13 mutants tested, only four (163N, 168Y, 234C and 248W) were completely negative for growth arrest whereas the remaining nine mutants exhibited reproducible growth suppressor activity (Figure 2). Mutants with wild-ty ...
PDF
... Citation: Garrido-Allepuz C, González-Lamuño D, Ros MA (2012) Sirenomelia Phenotype in Bmp7;Shh Compound Mutants: A Novel Experimental Model for Studies of Caudal Body Malformations. PLoS ONE 7(9): e44962. doi:10.1371/journal.pone.0044962 ...
... Citation: Garrido-Allepuz C, González-Lamuño D, Ros MA (2012) Sirenomelia Phenotype in Bmp7;Shh Compound Mutants: A Novel Experimental Model for Studies of Caudal Body Malformations. PLoS ONE 7(9): e44962. doi:10.1371/journal.pone.0044962 ...
Sporulation in BhciZZus subtilis 168. Control of
... (1970) and adapted by Coote (rgpb). The lysates were used to transduce auxotrophic strains, essentially as described by Coote (1972 b). Transduced cultures and controls were plated on LP agar which lacked the appropriate auxotrophic requirement of the recipient strain. After incubation for 2 days at ...
... (1970) and adapted by Coote (rgpb). The lysates were used to transduce auxotrophic strains, essentially as described by Coote (1972 b). Transduced cultures and controls were plated on LP agar which lacked the appropriate auxotrophic requirement of the recipient strain. After incubation for 2 days at ...
i A Thesis Entitled A Visual Screen for Centriolar Mutants in
... CPAP, their localization is not restricted to the centriole’s proximal end (Blanchon 2009; Mottier-Pavie 2009). Mammalian Centrobin is a centriolar protein that prevents degradation of CPAP. Therefore mutations in human Centrobin indirectly cause short centrioles through loss of centriolar CPAP (Gud ...
... CPAP, their localization is not restricted to the centriole’s proximal end (Blanchon 2009; Mottier-Pavie 2009). Mammalian Centrobin is a centriolar protein that prevents degradation of CPAP. Therefore mutations in human Centrobin indirectly cause short centrioles through loss of centriolar CPAP (Gud ...
Jaw and branchial arch mutants in zebrafish I: branchial
... Two large mutant classes emerged initially: the ‘flatheadgroup’, with reduced eyes, brain and jaw, and the ‘hammerhead group’, with reduced jaws but normal eyes and brain (Table 1). These and other mutations were classified to groups according to their live phenotypes and skeletal preparations. We h ...
... Two large mutant classes emerged initially: the ‘flatheadgroup’, with reduced eyes, brain and jaw, and the ‘hammerhead group’, with reduced jaws but normal eyes and brain (Table 1). These and other mutations were classified to groups according to their live phenotypes and skeletal preparations. We h ...
The axr4 auxin-resistant mutants of Arabidopsis thaliana define a
... the growing root. In many plants, very low concentrations of auxin stimulate root elongation, while higher concentrations invariably inhibit elongation (Burstr(]m, 1969; Evans et al., 1994). Root gravitropism is thought to involve the effects of auxin on cell elongation. According to the Cholodney-W ...
... the growing root. In many plants, very low concentrations of auxin stimulate root elongation, while higher concentrations invariably inhibit elongation (Burstr(]m, 1969; Evans et al., 1994). Root gravitropism is thought to involve the effects of auxin on cell elongation. According to the Cholodney-W ...
Granato 1996
... unbundled (fub), result in completely paralyzed embryos. The mutant phenotypes become visible by 20 hours, as spontaneous muscle contractions become apparent in wild-type embryos but not in mutant embryos. Mutant embryos remain completely immotile and fail to hatch from their chorions. Birefringency ...
... unbundled (fub), result in completely paralyzed embryos. The mutant phenotypes become visible by 20 hours, as spontaneous muscle contractions become apparent in wild-type embryos but not in mutant embryos. Mutant embryos remain completely immotile and fail to hatch from their chorions. Birefringency ...
How to test for complementation between mutant strains. David D. Perkins Background
... "Heterokaryon tests were made with heavy conidial suspensions of the individual mutant strains. Approximately 0.03 ml of the individual conidial suspensions was placed in spots near the periphery of petri plates previously prepared with 15−20 ml of minimal medium solidified with 1.0% agar. Each plat ...
... "Heterokaryon tests were made with heavy conidial suspensions of the individual mutant strains. Approximately 0.03 ml of the individual conidial suspensions was placed in spots near the periphery of petri plates previously prepared with 15−20 ml of minimal medium solidified with 1.0% agar. Each plat ...
THE GENETICS OF CAENORΉABDITIS ELEGANS
... carried out without much success. DR. J. SULSTON discovered that the worms could be stored in liquid nitrogen, provided that glycerol was present and that the initial squeezing took place slowly. The standard method used is as follows: worms are washed off the surface of a petri dish culture using a ...
... carried out without much success. DR. J. SULSTON discovered that the worms could be stored in liquid nitrogen, provided that glycerol was present and that the initial squeezing took place slowly. The standard method used is as follows: worms are washed off the surface of a petri dish culture using a ...
Analyses of Mutants of Three Genes that Influence Root Hair
... to the egg, the resulting hypoploid embryos would yield mutant seedlings. In contrast, no mutants will be recovered from crosses between female parents that carry a mutant that is not included on the translocated segment. Hence, if plants carrying a given mutant are crossed by all 19 BA stocks it is ...
... to the egg, the resulting hypoploid embryos would yield mutant seedlings. In contrast, no mutants will be recovered from crosses between female parents that carry a mutant that is not included on the translocated segment. Hence, if plants carrying a given mutant are crossed by all 19 BA stocks it is ...
The genetics of Caenorhabditis elegans.
... stored in liquid nitrogen, provided that glycerol was present and that the initial freezing took place slowly. The standard method used is as follows: worms are washed off the surface of a petri dish culture using about 1.5 m l of S o r M 9 buffer. To 1 ml of this suspension is added 1 ml of a 30% s ...
... stored in liquid nitrogen, provided that glycerol was present and that the initial freezing took place slowly. The standard method used is as follows: worms are washed off the surface of a petri dish culture using about 1.5 m l of S o r M 9 buffer. To 1 ml of this suspension is added 1 ml of a 30% s ...
Anaerobic Growth of Escherichia coli K12 with
... Two nutritionally distinct groups were detected. The major group (13 mutants) responded to 1,4-dihydroxy-2-naphthoate(DHN), 2-succinylbenzoate (SB) and its dilactone, whereas the minor group (2 mutants) only responded to DHN. DHN was at least five times more effective than SB but it inhibited growth ...
... Two nutritionally distinct groups were detected. The major group (13 mutants) responded to 1,4-dihydroxy-2-naphthoate(DHN), 2-succinylbenzoate (SB) and its dilactone, whereas the minor group (2 mutants) only responded to DHN. DHN was at least five times more effective than SB but it inhibited growth ...
hirota - Genetics
... containing these mutations were pili+ and others were pili-. That such phenotypically similar mutants were obtained as deletion mutations may suggest the presence of subunits in cistron C which probably control piliation. The pili+ mutants having deletions for all of cistron A and at the same time, ...
... containing these mutations were pili+ and others were pili-. That such phenotypically similar mutants were obtained as deletion mutations may suggest the presence of subunits in cistron C which probably control piliation. The pili+ mutants having deletions for all of cistron A and at the same time, ...
Functional cooperation between the non-paralogous
... 1986), (ii) all lumbar vertebrae are very similar to each other, lateral and spinous processes (the more useful features for vertebral rank identification) having a smooth gradient of size and shape from the first lumbar vertebra to the last one, and (iii) these differences between normal lumbar ver ...
... 1986), (ii) all lumbar vertebrae are very similar to each other, lateral and spinous processes (the more useful features for vertebral rank identification) having a smooth gradient of size and shape from the first lumbar vertebra to the last one, and (iii) these differences between normal lumbar ver ...
Adventitious root formation in rice requires OsGNOM1 and
... In contrast to the tap root system of Arabidopsis thaliana, monocot cereals consist almost entirely of a complex fibrous root system, comprising a mass of adventitious roots (ARs) that develop from the stem base postembryonically. Rice (Oryza sativa L.) is a model cereal crop with seminal roots that ...
... In contrast to the tap root system of Arabidopsis thaliana, monocot cereals consist almost entirely of a complex fibrous root system, comprising a mass of adventitious roots (ARs) that develop from the stem base postembryonically. Rice (Oryza sativa L.) is a model cereal crop with seminal roots that ...
Transductional Analysis of Arginineless Mutants in Proteus rnirabilis
... were sometimes present in transducing lysates. The effect of these virulent phages on the transduction frequency was tested in reconstruction experiments as follows : Phage 13vir was prepared by the method of Prozesky et al. (1965).Dilutions of the 13vir lysates were made in a lysate of transducing ...
... were sometimes present in transducing lysates. The effect of these virulent phages on the transduction frequency was tested in reconstruction experiments as follows : Phage 13vir was prepared by the method of Prozesky et al. (1965).Dilutions of the 13vir lysates were made in a lysate of transducing ...
Käfer, E. and D. Luk
... Results for UV, mitomycin C (MC) and HIS are summarized in Table 1. All new mutants were found to be cross-sensitive to some mutagenic agents and are, therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induc ...
... Results for UV, mitomycin C (MC) and HIS are summarized in Table 1. All new mutants were found to be cross-sensitive to some mutagenic agents and are, therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induc ...
lac
... -lacI-d mutations alter LacI such that it binds the operator even in the presence of inducer and prevents expression of the operon ...
... -lacI-d mutations alter LacI such that it binds the operator even in the presence of inducer and prevents expression of the operon ...
c. MUTANTS OF PARAMECIUM DEFECTIVE IN CHEMOKINESIS
... wild-type phenotypes appears in crosses of two separate fol' lines, it is possible that there is some suppression or other interaction between folZd and fol' gene products. However, the intergenic suppression is not complete because the crosses of folZdwith fol'* and fol" fail to fit a 1:l ratio at ...
... wild-type phenotypes appears in crosses of two separate fol' lines, it is possible that there is some suppression or other interaction between folZd and fol' gene products. However, the intergenic suppression is not complete because the crosses of folZdwith fol'* and fol" fail to fit a 1:l ratio at ...
RESEARCH NOTES
... fragment of the resulting colony was removed and grown on a Fries complete agar slant for later analysis. To select MBC resistant mutants, DMS mutagenized conidia were resuspended in liquid Fries minimal medium containing 1.5% sucrose (w/v) and swirled overnight at room temperature. The suspension w ...
... fragment of the resulting colony was removed and grown on a Fries complete agar slant for later analysis. To select MBC resistant mutants, DMS mutagenized conidia were resuspended in liquid Fries minimal medium containing 1.5% sucrose (w/v) and swirled overnight at room temperature. The suspension w ...