Folding minimal sequences: the lower bound for sequence
... two reduced-alphabet sequences: a simpli¢ed sarc homology 3 (SH3) domain [3] and a designed helical protein, DHP1 [5]. Simpli¢ed SH3 domains were selected by their biological binding function and characterized by circular dichroism and other methods as folding into a structure similar to that found ...
... two reduced-alphabet sequences: a simpli¢ed sarc homology 3 (SH3) domain [3] and a designed helical protein, DHP1 [5]. Simpli¢ed SH3 domains were selected by their biological binding function and characterized by circular dichroism and other methods as folding into a structure similar to that found ...
HeLa cells
... The main question - Does MKP-1 play a role in regulating transcriptional activation in response to genotoxic agents? ...
... The main question - Does MKP-1 play a role in regulating transcriptional activation in response to genotoxic agents? ...
Ch 45 Notes
... • Epinephrine binds to receptors on the plasma membrane of liver cells. • This triggers the release of messenger molecules that activate enzymes and result in the release of glucose into the ...
... • Epinephrine binds to receptors on the plasma membrane of liver cells. • This triggers the release of messenger molecules that activate enzymes and result in the release of glucose into the ...
DOC - National Center for Macromolecular Imaging
... Why Studying Large Protein Complexes is Crucial at this Time DAVID G. DRUBIN Large protein complexes are important for virtually every biological process and most disease states. Complete genome sequences have revealed 10,000s of genes in a variety of different organisms. The daunting task of under ...
... Why Studying Large Protein Complexes is Crucial at this Time DAVID G. DRUBIN Large protein complexes are important for virtually every biological process and most disease states. Complete genome sequences have revealed 10,000s of genes in a variety of different organisms. The daunting task of under ...
Cell membrane phospholipids
... Production of second messengers Digestion of phospholipids by pancreatic juice Pathogenic bacteria degrade phospholipids of membranes spread of infection 2- Remodeling of phospholipids: Specific phospholipase removes fatty acid from phospholipid. Replacement of fatty acid by alternative fatty acid ...
... Production of second messengers Digestion of phospholipids by pancreatic juice Pathogenic bacteria degrade phospholipids of membranes spread of infection 2- Remodeling of phospholipids: Specific phospholipase removes fatty acid from phospholipid. Replacement of fatty acid by alternative fatty acid ...
ESCHERICHIA COLI
... to interspecies [13]. Humans use secondary metabolites in the form of herbal and recreational drugs as well as medicines. In recent years, the use of some secondary metabolites as an alternative to conventional antibiotics has generated the interest in human health research. More than 13,000 seconda ...
... to interspecies [13]. Humans use secondary metabolites in the form of herbal and recreational drugs as well as medicines. In recent years, the use of some secondary metabolites as an alternative to conventional antibiotics has generated the interest in human health research. More than 13,000 seconda ...
GPR17 shRNA Plasmid (r): sc-270023-SH
... G protein-coupled receptor 17, GPR17, also known as uracil nucleotide/cysteinyl leukotriene receptor or P2Y-like receptor (P2YL), is a 367 amino acid member of the G protein-coupled receptor 1 family of proteins. While GPR17 is expressed in kidney, heart and umbilical vein endothelial cells, it is e ...
... G protein-coupled receptor 17, GPR17, also known as uracil nucleotide/cysteinyl leukotriene receptor or P2Y-like receptor (P2YL), is a 367 amino acid member of the G protein-coupled receptor 1 family of proteins. While GPR17 is expressed in kidney, heart and umbilical vein endothelial cells, it is e ...
Cytochrome P450 3A4: The Impossible Protein
... this situation the drugs will not be oxidized in the same way that they would be if there were only one bound to the protein. This makes it difficult to administer more than one drug at once because of the possible effects that occur due to 3A4. In addition to having this binding property, 3A4 is t ...
... this situation the drugs will not be oxidized in the same way that they would be if there were only one bound to the protein. This makes it difficult to administer more than one drug at once because of the possible effects that occur due to 3A4. In addition to having this binding property, 3A4 is t ...
Protein folding: looping from hydrophobic nuclei.
... expectation we compared sequence locations of the loop ends1 in nine major folds8 with positions of hydrophobic clusters in their sequences. These clusters were identified by Kyte & Doolitlle procedure9 as individual peaks in the hydropathicity plots. Figure 2 presents pairwise comparisons of the hy ...
... expectation we compared sequence locations of the loop ends1 in nine major folds8 with positions of hydrophobic clusters in their sequences. These clusters were identified by Kyte & Doolitlle procedure9 as individual peaks in the hydropathicity plots. Figure 2 presents pairwise comparisons of the hy ...
Bacterial Systems for Assembly, Secretion and Targeted
... intestinal epithelial cells (Fig. 1F). A Type III system is also used by Pseudomonas syringae and other plant pathogens to deliver a set of proteins, called avirulence proteins, which induce apoptosis in the host cells (7). The type III system is the most complex bacterial protein secretion system. ...
... intestinal epithelial cells (Fig. 1F). A Type III system is also used by Pseudomonas syringae and other plant pathogens to deliver a set of proteins, called avirulence proteins, which induce apoptosis in the host cells (7). The type III system is the most complex bacterial protein secretion system. ...
protein expression after nacl treatment in two tomato cultivars
... Na+/H+ antiporter that mediates transport of Na+ and K+ into the vacuole (Sottosanto et al., 2007). Myosin mutants are defective in both organelle movement and polar auxin transport through the action on several vesicle-mediated processes Holweg and Nick (2004). The expression patterns of the protei ...
... Na+/H+ antiporter that mediates transport of Na+ and K+ into the vacuole (Sottosanto et al., 2007). Myosin mutants are defective in both organelle movement and polar auxin transport through the action on several vesicle-mediated processes Holweg and Nick (2004). The expression patterns of the protei ...
Adding Protein Context to the Human Protein-Protein
... connected all proteins annotated with the GO terms ‘receptor’ and ‘sequence-specific DNA binding transcription factor activity’, respectively, in the UniprotKB [23]. This is done assuming that signal pathways follow the transmission of information through interacting proteins starting in cell surfac ...
... connected all proteins annotated with the GO terms ‘receptor’ and ‘sequence-specific DNA binding transcription factor activity’, respectively, in the UniprotKB [23]. This is done assuming that signal pathways follow the transmission of information through interacting proteins starting in cell surfac ...
lecture 7
... the native state OR Hsp70 may ‘transfer’ non-native proteins to other chaperones for folding (e.g., chaperonins) Hsp70 is also important during cellular stresses (thermotolerance), and has numerous other functions in the cell apart from assisting de novo protein folding. It often works in collabor ...
... the native state OR Hsp70 may ‘transfer’ non-native proteins to other chaperones for folding (e.g., chaperonins) Hsp70 is also important during cellular stresses (thermotolerance), and has numerous other functions in the cell apart from assisting de novo protein folding. It often works in collabor ...
PPT
... Only main-chain heavy atoms and Cbeta-atom of sidechains are taken into account, Bond lengths and bond angles are held constant and correspond to the alanine geometry. The only remaining geometrical variables are the backbone torsion angles. ...
... Only main-chain heavy atoms and Cbeta-atom of sidechains are taken into account, Bond lengths and bond angles are held constant and correspond to the alanine geometry. The only remaining geometrical variables are the backbone torsion angles. ...
Recognition of Metal Ion Binding Proteins
... This experiment indicates that a combination of C4.5 classifier and Correlation-based feature selection has great potential in detecting specific metal-binding protein classes especially Sodium and Nickel binding proteins. Furthermore it also indicates that there exists a general fingerprint common ...
... This experiment indicates that a combination of C4.5 classifier and Correlation-based feature selection has great potential in detecting specific metal-binding protein classes especially Sodium and Nickel binding proteins. Furthermore it also indicates that there exists a general fingerprint common ...
Lecture 6 Thurs 4-13-06
... Another protein (guanine-nuc. exchange factor) catalyzes exchange of GDP bound to cytosolic Rab for GTP, which allows Rab to bind to the transport vesicle. ...
... Another protein (guanine-nuc. exchange factor) catalyzes exchange of GDP bound to cytosolic Rab for GTP, which allows Rab to bind to the transport vesicle. ...
Genomics of Theileria parva
... • Can include the cleavage of the pro- region to release the active protein, the removal of the signal peptide and numerous covalent modifications such as, acetylations, glycosylations, hydroxylations, methylations and phosphorylations. • Posttranslational modifications may alter the molecular weigh ...
... • Can include the cleavage of the pro- region to release the active protein, the removal of the signal peptide and numerous covalent modifications such as, acetylations, glycosylations, hydroxylations, methylations and phosphorylations. • Posttranslational modifications may alter the molecular weigh ...
Gene Section STK11 (serine/threonine kinase 11) Atlas of Genetics and Cytogenetics
... Many of the polyps that develop in Peutz-Jeghers syndrome (see below) show loss of heterozygosity; somatic mutations have been tested and rarely found in the following sporadic cancers: pancreas, colon, stomach, breast, uterine cervix, ovary, testis, melanomas. The inactivation of the LKB1can also o ...
... Many of the polyps that develop in Peutz-Jeghers syndrome (see below) show loss of heterozygosity; somatic mutations have been tested and rarely found in the following sporadic cancers: pancreas, colon, stomach, breast, uterine cervix, ovary, testis, melanomas. The inactivation of the LKB1can also o ...
Protein Folding
... for large protein, long range contacts are important; cooperativity between the shortrange initiation and long range contacts lead to efficient folding. (In fact, helical protein tend to fold faster than b sheet protein) A core in large systems may occur independently in different regions, resulting ...
... for large protein, long range contacts are important; cooperativity between the shortrange initiation and long range contacts lead to efficient folding. (In fact, helical protein tend to fold faster than b sheet protein) A core in large systems may occur independently in different regions, resulting ...
The Number of Protein Subunits Per Helix Turn in Narcissus Mosaic
... By combining the results of Fourier transform calculations from digitized electron micrographs and molecular volume calculations based on the amino acid composition and RNA content of narcissus mosaic virus particles, firm evidence that there are 8-8 protein subunits per turn of the helix in the vir ...
... By combining the results of Fourier transform calculations from digitized electron micrographs and molecular volume calculations based on the amino acid composition and RNA content of narcissus mosaic virus particles, firm evidence that there are 8-8 protein subunits per turn of the helix in the vir ...
Structure and function of the chloroplast signal recognition particle
... formation with cpSRP. By analogy, with the co-translational targeting system where binding of cytosolic SRP54 to a substrate protein is mediated via its hydrophobic signal sequence, it was shown that a hydrophobic domain within LHCP is required for the post-translational binding to cpSRP (DeLille et ...
... formation with cpSRP. By analogy, with the co-translational targeting system where binding of cytosolic SRP54 to a substrate protein is mediated via its hydrophobic signal sequence, it was shown that a hydrophobic domain within LHCP is required for the post-translational binding to cpSRP (DeLille et ...
Protein Interaction Analysis - Bio-Rad
... Even if a protein and its ligand exhibit strong binding affinity for each other and are expressed at sufficient levels in a particular tissue to interact functionally, other factors influence the nature of their interaction. Certain molecules, such as ions or cofactors, may be critical for binding, ...
... Even if a protein and its ligand exhibit strong binding affinity for each other and are expressed at sufficient levels in a particular tissue to interact functionally, other factors influence the nature of their interaction. Certain molecules, such as ions or cofactors, may be critical for binding, ...
Full Text
... volatile anesthetics in the LGICs are the cavities formed within the core of transmembrane four-helix bundles. For many years since the era of Meyer and Overton,1,2 the mechanisms of anesthetic action were thought to be mediated via the lipid bilayer of cells involved in neuronal pathways fundamenta ...
... volatile anesthetics in the LGICs are the cavities formed within the core of transmembrane four-helix bundles. For many years since the era of Meyer and Overton,1,2 the mechanisms of anesthetic action were thought to be mediated via the lipid bilayer of cells involved in neuronal pathways fundamenta ...
PDF file
... proteins that form a heterodimer complex. The amino acid sequence of the catalytic region of yeast RTPase subunit has no obvious similarity to PTPs [9,10]. Unlike metazoan RTPases, the yeast proteins require divalent cations for activity [11,12]. It has been suggested that fungal RTPases comprise a ...
... proteins that form a heterodimer complex. The amino acid sequence of the catalytic region of yeast RTPase subunit has no obvious similarity to PTPs [9,10]. Unlike metazoan RTPases, the yeast proteins require divalent cations for activity [11,12]. It has been suggested that fungal RTPases comprise a ...
G protein–coupled receptor
G protein–coupled receptors (GPCRs), also known as seven-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptor, and G protein–linked receptors (GPLR), constitute a large protein family of receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. Coupling with G proteins, they are called seven-transmembrane receptors because they pass through the cell membrane seven times.G protein–coupled receptors are found only in eukaryotes, including yeast, choanoflagellates, and animals. The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. G protein–coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. Two of the United States's top five selling drugs (Hydrocodone and Lisinopril) act by targeting a G protein–coupled receptor. The 2012 Nobel Prize in Chemistry was awarded to Brian Kobilka and Robert Lefkowitz for their work that was ""crucial for understanding how G protein–coupled receptors function."". There have been at least seven other Nobel Prizes awarded for some aspect of G protein–mediated signaling.There are two principal signal transduction pathways involving the G protein–coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. When a ligand binds to the GPCR it causes a conformational change in the GPCR, which allows it to act as a guanine nucleotide exchange factor (GEF). The GPCR can then activate an associated G protein by exchanging its bound GDP for a GTP. The G protein's α subunit, together with the bound GTP, can then dissociate from the β and γ subunits to further affect intracellular signaling proteins or target functional proteins directly depending on the α subunit type (Gαs, Gαi/o, Gαq/11, Gα12/13).