Calcium Signals from the Vacuole
... certain developmental stage and when a slight hypoosmolar stress is applied to isolated vacuoles. Since the pioneering work on InsP3-dependent vacuolar Ca2+ release in the late 1980s and early 1990s little new experimental work on this important topic has been reported. The answer to the question if ...
... certain developmental stage and when a slight hypoosmolar stress is applied to isolated vacuoles. Since the pioneering work on InsP3-dependent vacuolar Ca2+ release in the late 1980s and early 1990s little new experimental work on this important topic has been reported. The answer to the question if ...
SACpaper-TMG12-27 FS..
... be a member of the TRP family of receptors. Blocking Ca2+ influx through other channel types has little or opposite effects. The growth-promoting effects induced by blocking specific Ca2+ influx pathways occurs for neurons growing upon a variety of biological and non-biological substrata, as well as ...
... be a member of the TRP family of receptors. Blocking Ca2+ influx through other channel types has little or opposite effects. The growth-promoting effects induced by blocking specific Ca2+ influx pathways occurs for neurons growing upon a variety of biological and non-biological substrata, as well as ...
Potentiation of acid-sensing ion channels by sulfhydryl compounds
... ACID-SENSING ION CHANNELS (ASICs) are members of the degenerin (DEG)/epithelial Na channel (ENaC) ion channel family. These voltage-independent ion channels are activated by extracellular acidosis and are expressed in neurons throughout the central and peripheral nervous system (46, 47). There are f ...
... ACID-SENSING ION CHANNELS (ASICs) are members of the degenerin (DEG)/epithelial Na channel (ENaC) ion channel family. These voltage-independent ion channels are activated by extracellular acidosis and are expressed in neurons throughout the central and peripheral nervous system (46, 47). There are f ...
store-operated calcium channels
... Prakriya M, Lewis RS. Store-Operated Calcium Channels. Physiol Rev 95: 1383–1436, 2015. Published September 23, 2015; doi:10.1152/physrev.00020.2014.—Storeoperated calcium channels (SOCs) are a major pathway for calcium signaling in virtually all metozoan cells and serve a wide variety of functions ...
... Prakriya M, Lewis RS. Store-Operated Calcium Channels. Physiol Rev 95: 1383–1436, 2015. Published September 23, 2015; doi:10.1152/physrev.00020.2014.—Storeoperated calcium channels (SOCs) are a major pathway for calcium signaling in virtually all metozoan cells and serve a wide variety of functions ...
Towards the Physics of Calcium Signalling in Plants
... One of the earliest models [18,19] of calcium signalling in plants was motivated by the observation that in the unicellular green alga Eremosphaera viridis, the presence of Sr+ or caffeine induces repetitive [Ca2+] spiking in the cytosol. The model consists of a system of ODEs that describes calcium ...
... One of the earliest models [18,19] of calcium signalling in plants was motivated by the observation that in the unicellular green alga Eremosphaera viridis, the presence of Sr+ or caffeine induces repetitive [Ca2+] spiking in the cytosol. The model consists of a system of ODEs that describes calcium ...
Characterization of Nifedipine Block of the Human Heart Delayed
... transiently transfected into HEK cells using the mammalian expression vector pRc/CMV. Cells expressing hKv1.5 were detected by cotransfecting cells with the vector pHook-1 (Invitrogen, San Diego, CA). This plasmid encoded the production of an antibody to the hapten phOX, which when expressed is disp ...
... transiently transfected into HEK cells using the mammalian expression vector pRc/CMV. Cells expressing hKv1.5 were detected by cotransfecting cells with the vector pHook-1 (Invitrogen, San Diego, CA). This plasmid encoded the production of an antibody to the hapten phOX, which when expressed is disp ...
Differential Excitability Modulation of Striatal Medium Neuron
... Fluo-4 (200 M). The Alexa 568 enabled detailed visualization of distal dendrites and spines, whereas the Ca 2⫹-sensitive indicator Fluo-4 reported Ca 2⫹ transients induced in these regions by the somatically generated bAPs. After eGFP phenotyping and patching, the internal solution was allowed to e ...
... Fluo-4 (200 M). The Alexa 568 enabled detailed visualization of distal dendrites and spines, whereas the Ca 2⫹-sensitive indicator Fluo-4 reported Ca 2⫹ transients induced in these regions by the somatically generated bAPs. After eGFP phenotyping and patching, the internal solution was allowed to e ...
Calcium diffusion models and transmitter release in
... only an approximately square law dependence between calcium influx and release for different size depolarizations? It is probably because increasing depolarization increases influx by opening more calcium channels and thus recruiting additional domains of transmitter release. It is helpful to recall ...
... only an approximately square law dependence between calcium influx and release for different size depolarizations? It is probably because increasing depolarization increases influx by opening more calcium channels and thus recruiting additional domains of transmitter release. It is helpful to recall ...
Mutational Analysis of Muscle Nicotinic Acetylcholine Receptor
... changes resulting from these mutations are designated by position, wild-type amino acid~mutant amino acid. The sequence of the deletion mutants are presented in Materials and Methods. ¢ High affinity BTX binding was assayed by toxin antitoxin immunoprecipitation of extracts from cells pulse labeled ...
... changes resulting from these mutations are designated by position, wild-type amino acid~mutant amino acid. The sequence of the deletion mutants are presented in Materials and Methods. ¢ High affinity BTX binding was assayed by toxin antitoxin immunoprecipitation of extracts from cells pulse labeled ...
Vacuolar calcium channels - Journal of Experimental Botany
... been a pleiotropic effect. When KCO1 was expressed in yeast, it formed a voltage-independent, Ca2+-activated, K+selective ion channel (Bihler et al., 2005). An Arabidopsis knock-out mutant lacking TPC1 (tpc1-2) does not show any SV channel activity, and TPC1-overexpressing lines have increased SV ch ...
... been a pleiotropic effect. When KCO1 was expressed in yeast, it formed a voltage-independent, Ca2+-activated, K+selective ion channel (Bihler et al., 2005). An Arabidopsis knock-out mutant lacking TPC1 (tpc1-2) does not show any SV channel activity, and TPC1-overexpressing lines have increased SV ch ...
Characterization of Mg -regulated TRPM7
... Data from different groups suggest that the altered function and/or regulation of TRPM7 channels could be implicated in pathological conditions such as anoxic cell death and ischaemia-reperfusion injury [5], arrhythmias [17], atria fibrillation [18], etc. However, it is important to note that TRPM7 ...
... Data from different groups suggest that the altered function and/or regulation of TRPM7 channels could be implicated in pathological conditions such as anoxic cell death and ischaemia-reperfusion injury [5], arrhythmias [17], atria fibrillation [18], etc. However, it is important to note that TRPM7 ...
Expression Profiles of High Voltage
... Voltage-activated Ca2⫹ channels (VACC) allow Ca2⫹ influx in response to membrane depolarization and mediate diverse neural functions, including transmitter release and excitability. In general, VACC are composed of an ␣1 subunit and differential auxiliary subunits (, ␣2␦, and ␥). Until now, molecul ...
... Voltage-activated Ca2⫹ channels (VACC) allow Ca2⫹ influx in response to membrane depolarization and mediate diverse neural functions, including transmitter release and excitability. In general, VACC are composed of an ␣1 subunit and differential auxiliary subunits (, ␣2␦, and ␥). Until now, molecul ...
Artk96_CyclGMPReview_2016 - Pure
... present in prokaryotes and eukaryotes. Mammals count 20 CN-PDEs. Surprisingly, although CN-PDE activity has been reported in several plant preparations, the protein(s) that carries out this function in plants is still unknown. Its identification is made difficult by the lack of canonical sequences f ...
... present in prokaryotes and eukaryotes. Mammals count 20 CN-PDEs. Surprisingly, although CN-PDE activity has been reported in several plant preparations, the protein(s) that carries out this function in plants is still unknown. Its identification is made difficult by the lack of canonical sequences f ...
as PDF
... Durell et al. proposed a 3-D structural model of amyloid channels obtained from a computer simulation of the secondary structure of AβP(1–40) in membranes that showed the aggregation of 5- to 8-mers to form pore-like structures on the membranes (Durell et al., 1994). Jang et al. established a model ...
... Durell et al. proposed a 3-D structural model of amyloid channels obtained from a computer simulation of the secondary structure of AβP(1–40) in membranes that showed the aggregation of 5- to 8-mers to form pore-like structures on the membranes (Durell et al., 1994). Jang et al. established a model ...
Cytoplasmic Unsaturated Free Fatty Acids Inhibit ATP
... rather than a protein. This study reports the successful identification of the endogenous inhibitor and its effect on KACh channel kinetics. The inhibitor was determined to be a lipid molecule in the cytoplasm. Tests of various lipid molecules on the KACh channel function showed that only unsaturate ...
... rather than a protein. This study reports the successful identification of the endogenous inhibitor and its effect on KACh channel kinetics. The inhibitor was determined to be a lipid molecule in the cytoplasm. Tests of various lipid molecules on the KACh channel function showed that only unsaturate ...
Anion-Channel Blockers Inhibit S-Type Anion
... As illustrated in Figure 4A, the anion-channel blockers probenecid and niflumic acid (Greger, 1991) also prevented ABA inhibition of stomatal opening in Commelina. The Ki for 50% reversal of ABA inhibition of stomatal opening is approximately 120 p~ for 9-AC (Fig. 3), 400 p~ for probenecid, and only ...
... As illustrated in Figure 4A, the anion-channel blockers probenecid and niflumic acid (Greger, 1991) also prevented ABA inhibition of stomatal opening in Commelina. The Ki for 50% reversal of ABA inhibition of stomatal opening is approximately 120 p~ for 9-AC (Fig. 3), 400 p~ for probenecid, and only ...
Endoplasmic reticulum potassium–hydrogen exchanger and small
... did not slow down backward Ca2+ leak through SERCA (supplementary material Fig. S4D). The role of K+/H+ exchange was confirmed in cardiac fibers by the external addition of the KHE, nigericin (30 mM), which was able to attenuate the inhibitory effect of propranolol (Fig. 4D). Another KHE inhibitor, ...
... did not slow down backward Ca2+ leak through SERCA (supplementary material Fig. S4D). The role of K+/H+ exchange was confirmed in cardiac fibers by the external addition of the KHE, nigericin (30 mM), which was able to attenuate the inhibitory effect of propranolol (Fig. 4D). Another KHE inhibitor, ...
Molecular Structure and Physiological Function of Chloride Channels
... (147) (shown at right). A, bottom: model of CLC Cl⫺ channel derived from threedimensional crystal structure of a bacterial CLC protein shows that the membrane-associated part of the protein is composed of 17 ␣-helices (helix A is not inserted into the membrane). Inspection of the crystal (131a) reve ...
... (147) (shown at right). A, bottom: model of CLC Cl⫺ channel derived from threedimensional crystal structure of a bacterial CLC protein shows that the membrane-associated part of the protein is composed of 17 ␣-helices (helix A is not inserted into the membrane). Inspection of the crystal (131a) reve ...
KATP channels and preconditioning - Philipps
... central mediator of preconditioning [34,50–53]. Liu et al. [54] even proposed that activation of PKC, which requires its translocation from the cytosol to anchoring proteins on the membrane cytoskeleton [55], produces the preconditioned state. This idea was supported by experiments showing that PKC ...
... central mediator of preconditioning [34,50–53]. Liu et al. [54] even proposed that activation of PKC, which requires its translocation from the cytosol to anchoring proteins on the membrane cytoskeleton [55], produces the preconditioned state. This idea was supported by experiments showing that PKC ...
Second Messengers Mediate Increases in Cytosolic Calcium in
... relative quantitative contributions of intracellular/extracellular stores cannot be gauged. Qualitatively, it is clear from our results that both types of Ca21 stores are involved, although the 8-Br- derivatives were less potent than the db- derivatives at concentrations that mediated a [Ca21]cyt in ...
... relative quantitative contributions of intracellular/extracellular stores cannot be gauged. Qualitatively, it is clear from our results that both types of Ca21 stores are involved, although the 8-Br- derivatives were less potent than the db- derivatives at concentrations that mediated a [Ca21]cyt in ...
Modulation of Jellyfish Potassium Channels by External Potassium
... In higher vertebrates, extracellular potassium concentrations are regulated as a result of homeostatic mechanisms at the organ, tissue, and cellular levels. In contrast, there are no known organs or tissues maintaining relatively constant extracellular potassium levels in lower metazoans such as the ...
... In higher vertebrates, extracellular potassium concentrations are regulated as a result of homeostatic mechanisms at the organ, tissue, and cellular levels. In contrast, there are no known organs or tissues maintaining relatively constant extracellular potassium levels in lower metazoans such as the ...
Article
... Neuronal voltage-gated Ca2+ channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca2+ channels is generating regenerative dendritic Ca2+ spikes. However, the Ca2+ dependent mechanisms used to create t ...
... Neuronal voltage-gated Ca2+ channels are involved in electrical signalling and in converting these signals into cytoplasmic calcium changes. One important function of voltage-gated Ca2+ channels is generating regenerative dendritic Ca2+ spikes. However, the Ca2+ dependent mechanisms used to create t ...
Sensory TRP Channel Interactions with Endogenous Lipids and
... Phe-Met-Arg-Phe-NH2 (FMRFamide) signaling [88]. In mammalian sensory physiology, NGF, prostaglandins (PGs) and bradykinin are the three major inflammatory pain mediators via affecting C-fiber functions, which often leads to hyperalgesia. NGF and PGs are known to only sensitize sensory excitability, ...
... Phe-Met-Arg-Phe-NH2 (FMRFamide) signaling [88]. In mammalian sensory physiology, NGF, prostaglandins (PGs) and bradykinin are the three major inflammatory pain mediators via affecting C-fiber functions, which often leads to hyperalgesia. NGF and PGs are known to only sensitize sensory excitability, ...
Protein Kinase C inhibits Kv1.1 potassium channel function
... in ventricular cells (36). In the Xenopus oocyte expression system, several types of recombinant, voltagegated K channels have been shown to be modulated by PKC (18, 19, 21, 23, 29), and in some cases there is evidence that the K channel protein is the substrate for PKC phosphorylation (2, 3, 4, 8). ...
... in ventricular cells (36). In the Xenopus oocyte expression system, several types of recombinant, voltagegated K channels have been shown to be modulated by PKC (18, 19, 21, 23, 29), and in some cases there is evidence that the K channel protein is the substrate for PKC phosphorylation (2, 3, 4, 8). ...
Physiological implications of the regulation of vacuolar
... However, even after dissipating the potential difference across endosome membranes with valinomycin, ATPase still depends on the presence of Cl- ions. These findings suggest a chloride binding site in an intramembrane pump sector. Chloride channels The underlying mechanisms for the differences in ch ...
... However, even after dissipating the potential difference across endosome membranes with valinomycin, ATPase still depends on the presence of Cl- ions. These findings suggest a chloride binding site in an intramembrane pump sector. Chloride channels The underlying mechanisms for the differences in ch ...