Microscopes - Biozentrum
... performance microscopes, the optical configuration of the objective lens and eyepiece are matched to give the best possible optical performance. This occurs most commonly with apochromatic objectives. The objective lens - a cylinder containing one or more lenses, typically made of glass, to collect ...
... performance microscopes, the optical configuration of the objective lens and eyepiece are matched to give the best possible optical performance. This occurs most commonly with apochromatic objectives. The objective lens - a cylinder containing one or more lenses, typically made of glass, to collect ...
robust and automatic vanishing points detection with their
... introduced by Barnard (Barnard, 1983) and that since then has been used, modified, and optimized by many different authors (Magee and Aggarwal,1984)(Shufelt, 1999). Other authors used other accumulation spaces using the Hough transform (Lutton et al., 1994)(Quan and Mohr, 1989)(Tuytelaars et al., 19 ...
... introduced by Barnard (Barnard, 1983) and that since then has been used, modified, and optimized by many different authors (Magee and Aggarwal,1984)(Shufelt, 1999). Other authors used other accumulation spaces using the Hough transform (Lutton et al., 1994)(Quan and Mohr, 1989)(Tuytelaars et al., 19 ...
A nanometer notch filter with high rejection and throughput
... particularly for high finesse systems. In Fig. 2 we show a comparison between two calculated reflection response functions for a Fabry-Perot with no tilt and the other tilted to accommodate a 0.4-nm shift from the notch wavelength at 694.3 nm. We incorporate the measured FE and the finite input cone ...
... particularly for high finesse systems. In Fig. 2 we show a comparison between two calculated reflection response functions for a Fabry-Perot with no tilt and the other tilted to accommodate a 0.4-nm shift from the notch wavelength at 694.3 nm. We incorporate the measured FE and the finite input cone ...
Variational Phase Imaging Using the Transport-of
... that translate the phase shifts into detectable intensity differences are phase contrast microscopy (PC) [7] and differential interference contrast (DIC) microscopy [8]. However, both DIC and PC images are challenging to segment (and to track) due to shade and halo artifacts (see Figure 1). This ess ...
... that translate the phase shifts into detectable intensity differences are phase contrast microscopy (PC) [7] and differential interference contrast (DIC) microscopy [8]. However, both DIC and PC images are challenging to segment (and to track) due to shade and halo artifacts (see Figure 1). This ess ...
Electrical Excitation of Surface Plasmons
... @! me c2 , since energy and momentum cannot be simultaneously conserved. One way to bridge this mismatch is to employ polariton modes (plasmons). They have the same energy as free space photons but arbitrarily high spatial localization (hence momentum), and can therefore couple efficiently to elec ...
... @! me c2 , since energy and momentum cannot be simultaneously conserved. One way to bridge this mismatch is to employ polariton modes (plasmons). They have the same energy as free space photons but arbitrarily high spatial localization (hence momentum), and can therefore couple efficiently to elec ...
Development of a Total Internal Reflection Illumination System for
... subunits share the same distribution. This method will be discussed first; ultimately, it was not successful, so an alternative method using laser illumination was studied, which uses temporal, rather than sp ...
... subunits share the same distribution. This method will be discussed first; ultimately, it was not successful, so an alternative method using laser illumination was studied, which uses temporal, rather than sp ...
University of Groningen CURVATURE MEASUREMENT
... et al. (1964, p. 223) found quite different values for R. However, re-evaluating their exemplary case with Eq. 1 yields a very satisfactory linear fit. Our results thus show that instead of factoring out the phase parameter p (Eq. 1) it is a much better approach to make a direct, linear fit to the s ...
... et al. (1964, p. 223) found quite different values for R. However, re-evaluating their exemplary case with Eq. 1 yields a very satisfactory linear fit. Our results thus show that instead of factoring out the phase parameter p (Eq. 1) it is a much better approach to make a direct, linear fit to the s ...
Intense switchable fluorescence in light wave coupled electrowetting
... 艌1 m thick. This thickness is needed since the evanescent portion of the propagating violet light penetrates (with exponential intensity decay) ⬃1 m into the low refractive index layer. LWC operation utilizes this effect of total internal reflection to turn the device OFF. As shown in Fig. 2(b) th ...
... 艌1 m thick. This thickness is needed since the evanescent portion of the propagating violet light penetrates (with exponential intensity decay) ⬃1 m into the low refractive index layer. LWC operation utilizes this effect of total internal reflection to turn the device OFF. As shown in Fig. 2(b) th ...
Broad Band Two-Dimensional Manipulation of Surface Plasmons
... recorded features was measured by an atomic force microscope (AFM, Dimension 3100, Veeco). The exposure results for both samples are presented in panels c and e of Figure 2, respectively. Clearly, onedimensional gratings which come from the SPP interference were obtained between the slits/edges in b ...
... recorded features was measured by an atomic force microscope (AFM, Dimension 3100, Veeco). The exposure results for both samples are presented in panels c and e of Figure 2, respectively. Clearly, onedimensional gratings which come from the SPP interference were obtained between the slits/edges in b ...
Parfocal and Critical Focus Zone
... focus accounting for the effects of turbulence, diffraction and other factors that spread the light of the star into a (Airy) disk. The practical (rather than theoretical) CFZ is 10-30% larger. The differences in focus for this system are within the CFZ. The word "system" is used because these varia ...
... focus accounting for the effects of turbulence, diffraction and other factors that spread the light of the star into a (Airy) disk. The practical (rather than theoretical) CFZ is 10-30% larger. The differences in focus for this system are within the CFZ. The word "system" is used because these varia ...
... ratio of image field to object field, kEkimg/kEkobj, with given lateral wave vector kx. Using the Fresnel formula of a stratified medium (18), we computed the optical transfer function of a silver superlens system, taking into consideration the absorption in silver and in the adjacent media (Fig. 3) ...
Focusing of light through a stratified medium: a practical
... The description of waves in focal regions has lead to numerous efforts by many authors (Reference 1 and references therein). The computation of the point spread function of the optical microscope has generated intensive work to establish theoretical models of image formation [2-10], mainly because o ...
... The description of waves in focal regions has lead to numerous efforts by many authors (Reference 1 and references therein). The computation of the point spread function of the optical microscope has generated intensive work to establish theoretical models of image formation [2-10], mainly because o ...
Schlieren Visualization
... The first task is to assemble and align the schlieren setup. This may seem tedious and might take some time to attain good alignment and focus, but a well-aligned setup will make all the difference. The instructions here are a summary of Chapter 8 of Settles [1]; the reader is referred there for a m ...
... The first task is to assemble and align the schlieren setup. This may seem tedious and might take some time to attain good alignment and focus, but a well-aligned setup will make all the difference. The instructions here are a summary of Chapter 8 of Settles [1]; the reader is referred there for a m ...
Computational photography with plenoptic camera and light field
... photographic films in most instances. The latest revolution is computational photography, which seeks to make image reconstruction computation an integral part of the image formation process; in this way, there can be new capabilities or better performance in the overall imaging system. A leading ef ...
... photographic films in most instances. The latest revolution is computational photography, which seeks to make image reconstruction computation an integral part of the image formation process; in this way, there can be new capabilities or better performance in the overall imaging system. A leading ef ...
Polarization spectra of excited-state-Mg(3p)--rare-gas
... produced by absorption of linearly polarized light tuned to the 3s 'S0~3p 'Pl resonance transition at collision-induced polarization spectra are measured in a +200-cm 285.2 nm. Detuning-dependent, range around the Mg 3p 'Pl ~5s 'So transition at 571.2 nm. The spectra correspond to probing transient ...
... produced by absorption of linearly polarized light tuned to the 3s 'S0~3p 'Pl resonance transition at collision-induced polarization spectra are measured in a +200-cm 285.2 nm. Detuning-dependent, range around the Mg 3p 'Pl ~5s 'So transition at 571.2 nm. The spectra correspond to probing transient ...
Soft X-ray tomography and cryogenic light microscopy - X
... images produced in these studies were, however, highly informative and helped guide the specimen-handling protocols and data collection strategies used in later work. After the commissioning of an XM-2 in 2008, the earlier work of Larabell and colleagues was quickly surpassed. In addition to having ...
... images produced in these studies were, however, highly informative and helped guide the specimen-handling protocols and data collection strategies used in later work. After the commissioning of an XM-2 in 2008, the earlier work of Larabell and colleagues was quickly surpassed. In addition to having ...
Principles of Electron Structure Research at Atomic Resolution
... reciprocal space can be reached. The answer for the study of three-dimensional Fourier transforms, almost trivial in the case of X-ray structure analysis, is either to rotate the specimen for a fixed wavelength or to change the wavelength for a given orientation. The first method has independently b ...
... reciprocal space can be reached. The answer for the study of three-dimensional Fourier transforms, almost trivial in the case of X-ray structure analysis, is either to rotate the specimen for a fixed wavelength or to change the wavelength for a given orientation. The first method has independently b ...
a PDF of the paper on this project published in the OCEANS 2005
... drag generating elements at the height of the sample volume. In addition, the components surrounding the sample are streamlined and designed to minimize the local flow disturbance. The data from the two digital cameras and other sensors are transmitted at 120 MB/s through a 1 km long, 250 µm diamete ...
... drag generating elements at the height of the sample volume. In addition, the components surrounding the sample are streamlined and designed to minimize the local flow disturbance. The data from the two digital cameras and other sensors are transmitted at 120 MB/s through a 1 km long, 250 µm diamete ...
18.1 Raman Scattering
... refractive index changes, while Raman scatter is due to time-dependent changes in polarizability • scattering cross-sections are used to compare signal strengths from Rayleigh, Raman and fluorescence • several Raman spectra are examined that show interesting features ...
... refractive index changes, while Raman scatter is due to time-dependent changes in polarizability • scattering cross-sections are used to compare signal strengths from Rayleigh, Raman and fluorescence • several Raman spectra are examined that show interesting features ...
Phase-only hologram generation based on integral imaging and its
... holography[2−4] . Since II provides a compact pickup process in which 3D information is captured under an incoherent light source, the high requirements of the traditional holography recording process can be alleviated. Many researchers verified the performance of hologram generated based on II usin ...
... holography[2−4] . Since II provides a compact pickup process in which 3D information is captured under an incoherent light source, the high requirements of the traditional holography recording process can be alleviated. Many researchers verified the performance of hologram generated based on II usin ...
The Principles of Microscopy Part 2
... SVM Professor of Cytomics Professor of Immunopharmacology & Biomedical Engineering Director, Purdue University Cytometry Laboratories, Purdue University These slides are intended for use in a lecture series. Copies of the slides are distributed and students encouraged to take their notes on these gr ...
... SVM Professor of Cytomics Professor of Immunopharmacology & Biomedical Engineering Director, Purdue University Cytometry Laboratories, Purdue University These slides are intended for use in a lecture series. Copies of the slides are distributed and students encouraged to take their notes on these gr ...
An Introduction to Pulsed Dye Lasers Introduction Light amplification
... state and not the ground state. The common red He-Ne laser operates by exciting He atoms that then transfer their energy during collisions into an excited state of the Ne atom (Figure 3). Laser emission is then created from a three level system in the Ne atom. This process is called sensitization, w ...
... state and not the ground state. The common red He-Ne laser operates by exciting He atoms that then transfer their energy during collisions into an excited state of the Ne atom (Figure 3). Laser emission is then created from a three level system in the Ne atom. This process is called sensitization, w ...
BioE 123 Teaching Material Stanford University
... you the importance of watching out for spherical aberrations, especially with highcurvature plano-convex lenses! Ray Tracing Rules This is the part you’ve been waiting for! In this section we learn how to follow light rays as they travel through optical systems and thus predict where images will be ...
... you the importance of watching out for spherical aberrations, especially with highcurvature plano-convex lenses! Ray Tracing Rules This is the part you’ve been waiting for! In this section we learn how to follow light rays as they travel through optical systems and thus predict where images will be ...
Fundamental Limits in Confocal Microscopy
... crucial to being able to detect enough photons to produce a statistically well-defined fluorescence image in a reasonable amount of time. At present, the total power of the narrow-band illumination needed to excite fluorescence that emerges from the best diskscanning instruments is at least an order ...
... crucial to being able to detect enough photons to produce a statistically well-defined fluorescence image in a reasonable amount of time. At present, the total power of the narrow-band illumination needed to excite fluorescence that emerges from the best diskscanning instruments is at least an order ...
OPTICAL FILTER COATINGS
... To apply the table to a specific filter, simply multiply by peak transmittance. Both minimum and maximum full bandwidths are shown at various normalized transmittance levels. The difference between minimum and maximum full bandwidths allows for spacer material choice and filter-to-filter variation. ...
... To apply the table to a specific filter, simply multiply by peak transmittance. Both minimum and maximum full bandwidths are shown at various normalized transmittance levels. The difference between minimum and maximum full bandwidths allows for spacer material choice and filter-to-filter variation. ...