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inducible protein expression in embryonic tick cell lines
inducible protein expression in embryonic tick cell lines

... Growth and survival of R. rickettsii in mammalian and tick cell lines To determine their relative ability to support rickettsial growth, R. rickettsii were inoculated in Vero76, ECV304, DALBE3 and IDE2 cells and incubated at 34°C. Cell sonicates were prepared at various times post-infection (p.i.) a ...
IMMUNITY AND IMMUNIZATION
IMMUNITY AND IMMUNIZATION

... It is widely distributed in the tissue fluids and are equally available in the intra and extravascular spaces. It can cross the placenta, and so it provides passive immunity to the ...
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... • small glycosylated proteins containing NUMEROUS binding sites to cells, signaling molecules, and other ECM components • e.g. fibronectin and laminin: important for adhesion of epithelial cells to the basal lamina via transmembrane integrin ...
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... How do the airways remain protected from pathogens during injury, when differentiated cells normally providing host defense function are damaged and BCs, which are far less “experienced” at mediating host– pathogen interactions, become directly exposed to the outside environment full of microbes? An ...
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Power Point Presentation
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... • Each consists of two different polypeptide chains • The tips of the chain form a variable (V) region; the rest is a constant (C) region ...
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... • Suppressive cytokines (TGFb) ...
Read more - Alliance for Cancer Gene Therapy
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... vaccine formulations for induction of protective immunity [4,5]. An extension of this logic would be that even single proteins contain many hundreds of antigenic epitopes, all of which are not necessary; whereas some may even be detrimental to the induction of protective immunity. This has created a ...
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... In the 1930s and 1940s, scientists were very interested in identifying the biochemical nature of the “transforming principle.” The candidate molecules were DNA, RNA, and protein. These molecules were candidates because we knew that nuclei contained chromosomes which are associated with phenotypes (t ...
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... have the same variable region. The class of Ig does not have anything to do with binding specificity. Differential polyadenylation and splicing of an mRNA leads to coexpression of sIgM and sIgD. To get chains other than μ and δ, switch recombination causes a VHDJH unit to be translocated from upstre ...
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... Penaeid shrimp aquaculture is an important economic activity worldwide. Nevertheless, shrimp production has been seriously affected by diseases, mostly those caused by viruses (Flegel, 2006) and Vibrio bacteria (Bachère, 2000). Shrimp resistance to invading organisms is strongly influenced by its im ...
and t-lymphocyte immune deficiencies
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... chlamydia or viruses) and are very challenging, or even impossible, to grow on artificial media. 2. Some diseases, such as tetanus, have variable signs and symptoms between patients. 3. Some diseases, such as pneumonia & nephritis, may be caused by a variety of microbes. 4. Some pathogens, such as S ...
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Cell culture and cell lines

... • Cells from many fishes readily tolerate centrifugation at 20oC or even higher but frictional heating coupled with high ambient temperature may injure cells from cold water fishes. Seeding density for cell lines • The seeding density for subcultures of cell lines will vary with the cell, the medium ...
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Molecular mimicry

Molecular mimicry is defined as the theoretical possibility that sequence similarities between foreign and self-peptides are sufficient to result in the cross-activation of autoreactive T or B cells by pathogen-derived peptides. Despite the promiscuity of several peptide sequences which can be both foreign and self in nature, a single antibody or TCR (T cell receptor) can be activated by even a few crucial residues which stresses the importance of structural homology in the theory of molecular mimicry. Upon the activation of B or T cells, it is believed that these ""peptide mimic"" specific T or B cells can cross-react with self-epitopes, thus leading to tissue pathology (autoimmunity). Molecular mimicry is a phenomenon that has been just recently discovered as one of several ways in which autoimmunity can be evoked. A molecular mimicking event is, however, more than an epiphenomenon despite its low statistical probability of occurring and these events have serious implications in the onset of many human autoimmune disorders. In the past decade the study of autoimmunity, the failure to recognize self antigens as ""self,"" has grown immensely. Autoimmunity is a result of a loss of immunological tolerance, the ability for an individual to discriminate between self and non-self. Growth in the field of autoimmunity has resulted in more and more frequent diagnosis of autoimmune diseases. Consequently, recent data show that autoimmune diseases affect approximately 1 in 31 people within the general population. Growth has also led to a greater characterization of what autoimmunity is and how it can be studied and treated. With an increased amount of research, there has been tremendous growth in the study of the several different ways in which autoimmunity can occur, one of which is molecular mimicry. The mechanism by which pathogens have evolved, or obtained by chance, similar amino acid sequences or the homologous three-dimensional crystal structure of immunodominant epitopes remains a mystery.
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