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BIO 10 Lecture 9 REPRODUCTION: MITOSIS AND MEIOSIS
BIO 10 Lecture 9 REPRODUCTION: MITOSIS AND MEIOSIS

... • Living organisms are “survival machines” for DNA molecules • DNA molecules carry the information that enables host cells to protect and perpetuate them ...
HIV-1 Reverse Transcriptase
HIV-1 Reverse Transcriptase

... HIV-1 Reverse Transcriptase Human immunodeficiency virus (HIV) is the etiologic agent causing the Acquired Immunodeficiency Syndrome (AIDS), the world's most deadly infectious disease. According to the World Health Organization the total number of people living with the human immunodeficiency virus ...
Microarray technique and Functional genomics
Microarray technique and Functional genomics

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third of four for Chapter 9
third of four for Chapter 9

... transfer any bacterial gene, the process is called generalized transduction ...


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Molecular taxonomy,use of modern methods in the identification of a
Molecular taxonomy,use of modern methods in the identification of a

... restriction enzymes to cleave the DNA of both species and the comparison of the two may reveal the similarity or dissimilarity between them. In PCR identification, a single segment of DNA is amplified. Such DNA segment can be a particular protein coding gene or rRNA gene or a portion of it. The ampl ...
Inherited Diseases PowerPoint
Inherited Diseases PowerPoint

... What are the FOUR bases While you found in DNA? wait… 2. What molecule does DNA code for? 3. What is the name for the shape of a DNA molecule? 4. How many chromosomes does a human have? 5. Why is DNA extraction important? ...
The Human Genome Project: the next decade
The Human Genome Project: the next decade

... genome. Sequences of the two smallest human chromosomes were published in late 1999 and of course the complete “draft” of the human genome early in 2001. Competition between private and public sequencing efforts generated acrimony but spurred progress, and was a factor in the sequence being complete ...
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Dr. Becker`s Review – Exam 4 Notes provided by Kadie Keen

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Gene therapy- Methods, Status and Limitations
Gene therapy- Methods, Status and Limitations

... • Identify more efficient ways to deliver the genes to the patients’ genetic material • Develop vectors that can specifically focus on the targeted cells • Ensure that vectors will successfully insert the desired genes into each of these target cells ...
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SBI4U: Molecular Genetics Unit Review

... c. DNA polymerase – adds DNA nucleotides to form new DNA molecule, also replaces RNA primers with DNA d. Single-stranded binding proteins – stabilizes DNA molecule e. DNA ligase – joins DNA fragments together f. RNA primase – creates RNA primer 14. Why is it beneficial to have multiple replication b ...
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Introduction to some basic features of genetic information
Introduction to some basic features of genetic information

... individual nucleotides that form long polynucleotide chains; this macromolecule is collectively called DNA. Each nucleotide consists of three parts: a nitrogen base, a pentose sugar, and a phosphate group (see Figure 2.1). DNA consists of four different base nucleotides: adenine, thymine, guanine, a ...
REVIEW UNIT 4 & 5: HEREDITY & MOLECULAR GENETICS SAMPLE QUESTIONS
REVIEW UNIT 4 & 5: HEREDITY & MOLECULAR GENETICS SAMPLE QUESTIONS

... c. AA and Aa 2. A form of vitamin D-resistant rickets, known as hypophosphatemia, is inherited as an Xlinked dominant trait. If a male with hypophosphatemia marries a normal female, which of the following predictions concerning their potential progeny would be true? (1990:44) a. All of their sons wo ...
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Figure 3 (Biorad Laboratories, Inc.)

... need to be turned on at the same time. Operons are composed of several important segments: 1. A promoter - nucleotide sequence where the RNA polymerase binds to begin transcription 2. An operator - nucleotide sequence near or within the promoter, which acts as a “switch,” by controlling access to th ...
Wrap up Genes and Expression
Wrap up Genes and Expression

... >BBS4 exon2 TAAAGTAACTCTATCACAATATGGATTTAATGGATTAATTGCATAATTGGTGAGCTACTG ATTATTCTTGTTATTTGGATGCTTCTTTAAGTTAGCAAGTTTATATTGTGGTGCTTCAAT ATAGACTACTTATTTCATTTCAGAGAACTCAATTTCCTGTATCTACTGAGTCTCAAAAAC CCCGGCAGAAAAAAGGTCTGTATGCAGTTTCATGGTATGTGTATGTTTGCACAGACAGAT TTCTCTTTTATTTATTTATTTATTTTTTTTTTTGGAGGCAGAGT ...
DNA Webquest - sciencewithskinner
DNA Webquest - sciencewithskinner

... 11. How many amino acids are found in a typical protein? __________ 12. Protein synthesis is completed when a) a special stop codon is reached b) the cell has no more amino acids available c) the ribosomes disintegrate d) the tRNA anticodons no longer match the mRNA codons Return to "A Science Odyss ...
Genetic Markers and linkage mapping - genomics-lab
Genetic Markers and linkage mapping - genomics-lab

... hemoglobin is formed from two alpha chains and two beta chains.) The scheme shown was worked out from a comparison of beta-globin genes from many different organisms. For example, the nucleotide sequences of the gammaG and gammaA genes are much more similar to each other than either of them is to th ...
Forensic Science: An Introduction
Forensic Science: An Introduction

... used enzymes to cut the DNA around these tandem repeat sites and then run them on a gel electrophoresis • A Southern blot was then performed and radioactive probes were hybridized to help visualize the RFLPs ...
Chromosomes, DNA, and Genes
Chromosomes, DNA, and Genes

... • Unique characteristics are the result of different proteins • There are many unique proteins that an organism has to make to live and survive – Closely related organisms may have genes that code for the same or similar proteins – Organisms that are not closely related share fewer genes than organi ...
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Genomic library



A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.
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