Diapositive 1 - Institut Pasteur
... Analyses of the genetic material of different species help understanding the similarity and differences between genomes, their evolution and the evolution of their genes. •Intra-genomic comparisons help understanding the degree of duplication (genome regions; genes) and genes organization,... •Inter ...
... Analyses of the genetic material of different species help understanding the similarity and differences between genomes, their evolution and the evolution of their genes. •Intra-genomic comparisons help understanding the degree of duplication (genome regions; genes) and genes organization,... •Inter ...
Welcome to Comp 665 - UNC Computational Genetics
... segments organized into structures called chromosomes • Chromosomes vary between different organisms. The DNA molecule may be circular or linear, and can contain from 10,000 to 1,000,000,000 nucleotides. • Simple single-cell organisms (prokaryotes, cells without nuclei such as bacteria) generally ha ...
... segments organized into structures called chromosomes • Chromosomes vary between different organisms. The DNA molecule may be circular or linear, and can contain from 10,000 to 1,000,000,000 nucleotides. • Simple single-cell organisms (prokaryotes, cells without nuclei such as bacteria) generally ha ...
DNA Questions #1
... 7) Fill in. There are two types of DNA found in your cells. The first type, ___nDNA__________ is found in the nucleus of your cells. It is in the form called ___chromosomes________, which are linear structures that are made of DNA and proteins. Each of your cells has ____46______(number) pieces of ...
... 7) Fill in. There are two types of DNA found in your cells. The first type, ___nDNA__________ is found in the nucleus of your cells. It is in the form called ___chromosomes________, which are linear structures that are made of DNA and proteins. Each of your cells has ____46______(number) pieces of ...
dna
... polymerase the DNA is created in short pieces called OKASAKI FRAGMENTS. Another enzyme called a DNA LIGASE takes these fragments and puts them together into what is called the LAGGING STRAND. ...
... polymerase the DNA is created in short pieces called OKASAKI FRAGMENTS. Another enzyme called a DNA LIGASE takes these fragments and puts them together into what is called the LAGGING STRAND. ...
Sequencing and Phylogeny - World Health Organization
... Sequencing Process to determine the exact order of nucleotides in DNA ...
... Sequencing Process to determine the exact order of nucleotides in DNA ...
RNA-seq presentation
... Go through the tutorial sheet. There are two tasks, both using Galaxy: – Reference-based transcript assembly and expression analysis without annotation using Galaxy ...
... Go through the tutorial sheet. There are two tasks, both using Galaxy: – Reference-based transcript assembly and expression analysis without annotation using Galaxy ...
Synergies between assisted reproduction technologies and
... when coupled to the FokI nuclease, create efficient geneediting tools [42–44]. The TALEN and CRISPR/Cas9 systems are easier to engineer and more reliable than the use of zinc finger nucleases [45]. In the presence of single- or double-stranded DNA homologous to the target sequence, these systems can ...
... when coupled to the FokI nuclease, create efficient geneediting tools [42–44]. The TALEN and CRISPR/Cas9 systems are easier to engineer and more reliable than the use of zinc finger nucleases [45]. In the presence of single- or double-stranded DNA homologous to the target sequence, these systems can ...
Manual: XL1-Blue Supercompetent Cells
... transformation and one tube is for the pUC18 control.) Preheat SOC medium to 42°C. 2. Thaw the cells on ice. When thawed, gently mix and aliquot 100 µl of cells into each of the two pre-chilled tubes. 3. Add 1.7 µl of the β-mercaptoethanol provided with this kit to each aliquot of cells. 4. Swirl th ...
... transformation and one tube is for the pUC18 control.) Preheat SOC medium to 42°C. 2. Thaw the cells on ice. When thawed, gently mix and aliquot 100 µl of cells into each of the two pre-chilled tubes. 3. Add 1.7 µl of the β-mercaptoethanol provided with this kit to each aliquot of cells. 4. Swirl th ...
Chapter 8 Lecture Notes
... d. Genetics: Study of what genes are, how they carry information, how information is expressed, how genes/DNA are replicated, and how they are passed from one generation to the next (or passed between organisms). e. Genomics: Molecular study of genomes. f. Genotype and phenotype. i. Genotype: Entire ...
... d. Genetics: Study of what genes are, how they carry information, how information is expressed, how genes/DNA are replicated, and how they are passed from one generation to the next (or passed between organisms). e. Genomics: Molecular study of genomes. f. Genotype and phenotype. i. Genotype: Entire ...
Biology 105 Midterm Exam 2
... 34. Human skin color is a polygenic trait. The more dominant alleles a person has, the darker their skin color is. If two people with the genotype AaBbCc married, their children would be: a. all dark skinned b. all light skinned c. all medium skinned d. anywhere along the continuum of skin color 35. ...
... 34. Human skin color is a polygenic trait. The more dominant alleles a person has, the darker their skin color is. If two people with the genotype AaBbCc married, their children would be: a. all dark skinned b. all light skinned c. all medium skinned d. anywhere along the continuum of skin color 35. ...
PDF (black and white)
... cross-pollinated true-breeding plants to carry out his experiment. What were Mendel's two experiments? In his first experiment, Mendel studied 7 characteristics. He performed crosses ...
... cross-pollinated true-breeding plants to carry out his experiment. What were Mendel's two experiments? In his first experiment, Mendel studied 7 characteristics. He performed crosses ...
Unit review questions
... 8. Define plasmid & tell how they’re used in genetic engineering. 10. What is the first step in genetically engineering insulin from bacterial cells? 11. What is cloning? 12. What is recombinant DNA? 13. A plasmid containing recombinant DNA is inserted into a host ____________called a ______________ ...
... 8. Define plasmid & tell how they’re used in genetic engineering. 10. What is the first step in genetically engineering insulin from bacterial cells? 11. What is cloning? 12. What is recombinant DNA? 13. A plasmid containing recombinant DNA is inserted into a host ____________called a ______________ ...
File
... State that, when genes are transferred between species, the amino acid sequence of polypeptides translated from them is unchanged because the genetic code is universal. Outline a basic technique used for gene transfer involving plasmids, a host cell (bacterium, yeast or other cell), restriction enzy ...
... State that, when genes are transferred between species, the amino acid sequence of polypeptides translated from them is unchanged because the genetic code is universal. Outline a basic technique used for gene transfer involving plasmids, a host cell (bacterium, yeast or other cell), restriction enzy ...
Document
... • We share several of these with our most recent evolutionary ancestors – There are several thousand in the human genome ...
... • We share several of these with our most recent evolutionary ancestors – There are several thousand in the human genome ...
Nucleotide Sequence Databases
... • REFERENCE: credits author(s) who initially determined the sequence; includes ...
... • REFERENCE: credits author(s) who initially determined the sequence; includes ...
ScrFl restriction/modification system from
... encode a type I1 restriction ENase, ScrFI, which recognizes the sequence 5' CCJNGG 3' and cleaves as indicated (Fitzgerald et al., 1982). Two constructs, pCI931m and pCI932m, were isolated from the chromosome of this strain ;each encoded a SmC MTase which conferred resistance to ScrFI digestion (Dav ...
... encode a type I1 restriction ENase, ScrFI, which recognizes the sequence 5' CCJNGG 3' and cleaves as indicated (Fitzgerald et al., 1982). Two constructs, pCI931m and pCI932m, were isolated from the chromosome of this strain ;each encoded a SmC MTase which conferred resistance to ScrFI digestion (Dav ...
Putting it all together: Finding the cystic fibrosis gene
... Putting it all together: Finding the cystic fibrosis gene • What did we know before getting started? – CF is inherited in a classically Mendelian fashion – More than 10 million Americans are unknowing, symptomless carriers of the defective CF gene. – An individual must inherit two defective CF gene ...
... Putting it all together: Finding the cystic fibrosis gene • What did we know before getting started? – CF is inherited in a classically Mendelian fashion – More than 10 million Americans are unknowing, symptomless carriers of the defective CF gene. – An individual must inherit two defective CF gene ...
Section 8.1 Power point
... 8.1 Identifying DNA as the Genetic Material Historical timeline of discovering DNA 1875 - 1953 • Although Gregor Mendel’s experiments with pea plants in the 1870’s led to the the new science of genetics, he was never able to answer an important question – “What are the “factors” that control heredi ...
... 8.1 Identifying DNA as the Genetic Material Historical timeline of discovering DNA 1875 - 1953 • Although Gregor Mendel’s experiments with pea plants in the 1870’s led to the the new science of genetics, he was never able to answer an important question – “What are the “factors” that control heredi ...
DNA Replication - OG
... Fingerprinting takes the DNA out of a cell and separates it. This will allow investigators to distinguish body cells of different individuals (since they are unlikely to have the same DNA) Cloning – take the DNA out of one of your cells then take the DNA out of a zygote (fertilized egg). Put the DNA ...
... Fingerprinting takes the DNA out of a cell and separates it. This will allow investigators to distinguish body cells of different individuals (since they are unlikely to have the same DNA) Cloning – take the DNA out of one of your cells then take the DNA out of a zygote (fertilized egg). Put the DNA ...
CSE 181 Project guidelines
... • Codon: The sequence of 3 nucleotides in DNA/RNA that encodes for a specific amino acid. • mRNA (messenger RNA): A ribonucleic acid whose sequence is complementary to that of a protein-coding gene in DNA. • Ribosome: The organelle that synthesizes polypeptides under the direction of mRNA • rRNA (ri ...
... • Codon: The sequence of 3 nucleotides in DNA/RNA that encodes for a specific amino acid. • mRNA (messenger RNA): A ribonucleic acid whose sequence is complementary to that of a protein-coding gene in DNA. • Ribosome: The organelle that synthesizes polypeptides under the direction of mRNA • rRNA (ri ...
Table of Contents
... • Gene libraries contain fragments of DNA from an organism’s genome. • Restriction enzymes are used to break chromosomes into fragments, which are inserted into vectors and taken up by host cells. ...
... • Gene libraries contain fragments of DNA from an organism’s genome. • Restriction enzymes are used to break chromosomes into fragments, which are inserted into vectors and taken up by host cells. ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.