PowerPoint 演示文稿 - 茶叶生物学与资源利用国家重点实验室
... caffeine/L. Furthermore, we selected adenosyl-nucleosidase, inosine-/guanosinenucleosidase and inosine-/guanosine-phospholipase from established tea genomic library, on the basis of semi-quantative PCR results separately amplified in leaf and root of tea plant, we isolated three candidate tea xantho ...
... caffeine/L. Furthermore, we selected adenosyl-nucleosidase, inosine-/guanosinenucleosidase and inosine-/guanosine-phospholipase from established tea genomic library, on the basis of semi-quantative PCR results separately amplified in leaf and root of tea plant, we isolated three candidate tea xantho ...
LOTUS-DB: an integrative and interactive database for
... from China, USA, Australia and Japan (4, 15). As the initial step to understand the myths of sacred lotus, our group’s genome sequence is acquired by shotgun approach with 94.2 Gb (101) illumina and 4.8 Gb (5.2) 454 sequence. The final genome assembly reaches to 804 Mb, which is 86.5% of the estim ...
... from China, USA, Australia and Japan (4, 15). As the initial step to understand the myths of sacred lotus, our group’s genome sequence is acquired by shotgun approach with 94.2 Gb (101) illumina and 4.8 Gb (5.2) 454 sequence. The final genome assembly reaches to 804 Mb, which is 86.5% of the estim ...
Document
... • For the design of media used for the production of cell mass, the determination of an elemental material balance is a useful exercise. • For defined media, the determination is a straightforward calculation from the components. • For complex media, Traders’ Co. and other manufacturers of complex n ...
... • For the design of media used for the production of cell mass, the determination of an elemental material balance is a useful exercise. • For defined media, the determination is a straightforward calculation from the components. • For complex media, Traders’ Co. and other manufacturers of complex n ...
Sequencing genomes
... Holly Grail of sequencing – complete human genome below $ 1000. 1st generation – Sanger dideoxy method 2nd generation – sequencing by synthesis (pyrosequencing) 3rd generation – single molecule sequencing ...
... Holly Grail of sequencing – complete human genome below $ 1000. 1st generation – Sanger dideoxy method 2nd generation – sequencing by synthesis (pyrosequencing) 3rd generation – single molecule sequencing ...
Human Identity Testing
... suspect. An example of the result is provided in Figure 11.2. Here, the left hand panel is for reference. It has electrophoretic bands for all of the major alleles. The right Figure 11.2: Major TH01 alleles after hand panel is a sample either from the electrophoresis and probe identification. crime ...
... suspect. An example of the result is provided in Figure 11.2. Here, the left hand panel is for reference. It has electrophoretic bands for all of the major alleles. The right Figure 11.2: Major TH01 alleles after hand panel is a sample either from the electrophoresis and probe identification. crime ...
DNA extraction from cheek cells protocol I mailed to you
... Each chromosome in the nucleus of a cell contains a very long molecule of DNA. If you stretched out the DNA found in one of your cells, it would be 2-3 meters long. To fit all of this DNA inside a tiny cell nucleus, the DNA is wrapped tightly around proteins. The enzyme in meat tenderizer is a prote ...
... Each chromosome in the nucleus of a cell contains a very long molecule of DNA. If you stretched out the DNA found in one of your cells, it would be 2-3 meters long. To fit all of this DNA inside a tiny cell nucleus, the DNA is wrapped tightly around proteins. The enzyme in meat tenderizer is a prote ...
Breeding - Farming Ahead
... DNA pedigree testing is the first step in molecular genetic technology and breeders who start recording the parentage of their sheep can take advantage of new genetic markers as they become available. The research team is using its parentage trials to measure important commercial traits such as flee ...
... DNA pedigree testing is the first step in molecular genetic technology and breeders who start recording the parentage of their sheep can take advantage of new genetic markers as they become available. The research team is using its parentage trials to measure important commercial traits such as flee ...
chapter 15 chromosomal basis of inheritance
... be inherited together. Genetic recombination – the production of offspring with new combinations of traits inherited from two parents. 2 types of offspring can be produced: Parental types – offspring matching a parental phenotype. Recombinants – offspring that do not match either parent’s phenot ...
... be inherited together. Genetic recombination – the production of offspring with new combinations of traits inherited from two parents. 2 types of offspring can be produced: Parental types – offspring matching a parental phenotype. Recombinants – offspring that do not match either parent’s phenot ...
Recombinant DNA WS
... 1. Sequence each event of creating protein using recombinant DNA in the correct order. Use the word bank to fill in the blanks in the sequencing cutouts. 2. Draw a picture to match the process, making sure to label your drawing with the underlined words from the cut-outs. Use a different color to re ...
... 1. Sequence each event of creating protein using recombinant DNA in the correct order. Use the word bank to fill in the blanks in the sequencing cutouts. 2. Draw a picture to match the process, making sure to label your drawing with the underlined words from the cut-outs. Use a different color to re ...
slides
... Primers are short, artificial DNA strands — often not more than 50 and usually only 18 to 25 base pairs long — that are complementary to the beginning or the end of the DNA fragment to be amplified. ...
... Primers are short, artificial DNA strands — often not more than 50 and usually only 18 to 25 base pairs long — that are complementary to the beginning or the end of the DNA fragment to be amplified. ...
Lec 08 - Development of e
... requiring time, but is also a source of errors in the data. Therefore, elaborate procedures for mutation detection have been developed in some eukaryotes . eg; Drosophila, maize etc. These procedures employ specific markers to facilitate the identification of chromosomes from the treated or irradiat ...
... requiring time, but is also a source of errors in the data. Therefore, elaborate procedures for mutation detection have been developed in some eukaryotes . eg; Drosophila, maize etc. These procedures employ specific markers to facilitate the identification of chromosomes from the treated or irradiat ...
7.03 Fall 2003 Problem Set #3 Solutions
... (b) Since none of the 100 Kanr tranductants were Lac+, we can conclude that Tn5 was never co-transduced with lac2+. This indicates that the distance between lac2- and Tn5 is at least one phage head (105 bp). We know from part (a) that Tn5 and lac1- are within one phage head since their cotransductio ...
... (b) Since none of the 100 Kanr tranductants were Lac+, we can conclude that Tn5 was never co-transduced with lac2+. This indicates that the distance between lac2- and Tn5 is at least one phage head (105 bp). We know from part (a) that Tn5 and lac1- are within one phage head since their cotransductio ...
2009 Hart and Grosbe.. - Evolution and Ecology | UC Davis
... prediction. In the best (and most recent) test, Roeding et al. (23) sampled 149 genes (11,168 codons) from the genome of a scorpion and aligned those sequences to orthologues from the genomes of 2 onychophorans and 17 insects, plus 12 crustaceans and 21 other chelicerates and myriapods. These data r ...
... prediction. In the best (and most recent) test, Roeding et al. (23) sampled 149 genes (11,168 codons) from the genome of a scorpion and aligned those sequences to orthologues from the genomes of 2 onychophorans and 17 insects, plus 12 crustaceans and 21 other chelicerates and myriapods. These data r ...
A whole-genome assembly of the domestic cow, Bos taurus
... Having an accurate assembly of the genome of an important species provides an invaluable substrate for future research. For example, studies of genetic diversity need a good reference genome in order to catalog differences in new strains or lineages. Expression analyses that sequence RNA from variou ...
... Having an accurate assembly of the genome of an important species provides an invaluable substrate for future research. For example, studies of genetic diversity need a good reference genome in order to catalog differences in new strains or lineages. Expression analyses that sequence RNA from variou ...
2.5.2 Heredity and Gene Expression
... Many of the proteins made by genes are enzymes. Genes control a cell because the enzymes they make control cell activities. Therefore, the role of a gene is to control a cell. Chromosome structure. Chromosomes are made of DNA (40%) and protein (60%). The DNA is a very long molecule. It is very coile ...
... Many of the proteins made by genes are enzymes. Genes control a cell because the enzymes they make control cell activities. Therefore, the role of a gene is to control a cell. Chromosome structure. Chromosomes are made of DNA (40%) and protein (60%). The DNA is a very long molecule. It is very coile ...
BMC Research Notes - FABI
... gene cluster region (Region-1) organization Comparative gene cluster region (Region-1) organization. Genes denoted by the same color (except light yellow) are from the same orthologous group (top COG hit). Light yellow = no COG assignment. Figures 2-4 were generated at the IMG website as detailed in ...
... gene cluster region (Region-1) organization Comparative gene cluster region (Region-1) organization. Genes denoted by the same color (except light yellow) are from the same orthologous group (top COG hit). Light yellow = no COG assignment. Figures 2-4 were generated at the IMG website as detailed in ...
LP - Columbia University
... B. Lysogenic Cycle See 17A, Sadava fig. 16.4 (13.3) or Becker Box 18A, esp. fig. 18A-4. 1. Integration. Some viruses can become part of the host chromosome by crossing over between the viral DNA and the bacterial DNA -- the process is parallel to the way a plasmid such as the F factor joins the bact ...
... B. Lysogenic Cycle See 17A, Sadava fig. 16.4 (13.3) or Becker Box 18A, esp. fig. 18A-4. 1. Integration. Some viruses can become part of the host chromosome by crossing over between the viral DNA and the bacterial DNA -- the process is parallel to the way a plasmid such as the F factor joins the bact ...
A Rapid Method for the Identification of Plasmid Desoxyribonucleic
... simplicity, and the variety of bacterial species it can be applied to. The migration rate was found to be inversely related to the logarithm of the plasmid mass in the 2- to 50-Md range in a 0.8% agarose gel as noted elsewhere (4) (data not shown). The technique has been used to identify plasmids pr ...
... simplicity, and the variety of bacterial species it can be applied to. The migration rate was found to be inversely related to the logarithm of the plasmid mass in the 2- to 50-Md range in a 0.8% agarose gel as noted elsewhere (4) (data not shown). The technique has been used to identify plasmids pr ...
17.1 – Isolating the Genetic Material
... → the nucleotide composition of DNA varies from one species to another → the nucleotide composition of DNA specimens taken from different animals of the same species is fairly constant → in DNA, the amount of adenine is always equal to the amount of thymine and the amount of guanine is always equal ...
... → the nucleotide composition of DNA varies from one species to another → the nucleotide composition of DNA specimens taken from different animals of the same species is fairly constant → in DNA, the amount of adenine is always equal to the amount of thymine and the amount of guanine is always equal ...
DNA and Chromatin
... oligonucleotide is listed starting from the 5' end, followed by the sequence of nitrogenous bases, ending with the 3' end. ...
... oligonucleotide is listed starting from the 5' end, followed by the sequence of nitrogenous bases, ending with the 3' end. ...
CYTOGENETICS AND MEDICAL GENETICS IN THE 1960s
... be 48. In 1956, Tjio and Levan exploited advances in tissue culture, the use of colchicine to arrest cells in metaphase, hypotonicity to disperse the chromosomes and to enhance the quality of the cell preparation for study. Working with cultures of embryonic fibroblasts, they first identified the co ...
... be 48. In 1956, Tjio and Levan exploited advances in tissue culture, the use of colchicine to arrest cells in metaphase, hypotonicity to disperse the chromosomes and to enhance the quality of the cell preparation for study. Working with cultures of embryonic fibroblasts, they first identified the co ...
Meiosis II
... * Offers another form of cell division that allows offspring to have same # of chromosomes as parents. • Meiosis = cell division process which produces gametes containing half the number of chromosomes as a parent’s body cell. * consists of two divisions: Meiosis I & II male gametes = sperm ...
... * Offers another form of cell division that allows offspring to have same # of chromosomes as parents. • Meiosis = cell division process which produces gametes containing half the number of chromosomes as a parent’s body cell. * consists of two divisions: Meiosis I & II male gametes = sperm ...
Genomes 3/e
... start/end of a gene based on mRNA Prerequisite: a M13 library clone spanning the gene end is available Use of S1 nuclease to trim dsDNA molecule Figure 5.14 Genomes 3 (© Garland Science 2007) ...
... start/end of a gene based on mRNA Prerequisite: a M13 library clone spanning the gene end is available Use of S1 nuclease to trim dsDNA molecule Figure 5.14 Genomes 3 (© Garland Science 2007) ...
Genomic library
A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly a population of Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector allows for easy amplification and retrieval of specific clones from the library for analysis.There are several kinds of vectors available with various insert capacities. Generally, libraries made from organisms with larger genomes require vectors featuring larger inserts, thereby fewer vector molecules are needed to make the library. Researchers can choose a vector also considering the ideal insert size to find a desired number of clones necessary for full genome coverage.Genomic libraries are commonly used for sequencing applications. They have played an important role in the whole genome sequencing of several organisms, including the human genome and several model organisms.