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Profile Documents Logout
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RNA-seq presentation
RNA-seq presentation

... Log-in using the yellow username on your machine. Go through the tutorial sheet. There are two tasks, both using Galaxy: – Reference-based transcript assembly and expression analysis without annotation using Galaxy ...
Chapter 10 lecture
Chapter 10 lecture

... ─ A population of cells with similar characteristics ─ Clone: Population of cells derived from a single cell ...
Taxonomy of Bacteria and Archaea
Taxonomy of Bacteria and Archaea

... Detailed phylogenetic tree of the major lineages (phyla) of Bacteria based on 16S ribosomal RNA sequence comparisons ...
Genomics – The Language of DNA
Genomics – The Language of DNA

... Alu elements consist of a sequence of 300 base pairs containing a site that is recognized by the restriction enzyme AluI. They appear to be reverse transcripts of 7S RNA, part of the signal recognition particle. Most SINEs do not encode any functional molecules and depend on the machinery of active ...
Validity of transferring the footprint sites identified in lab
Validity of transferring the footprint sites identified in lab

... Validity of transferring the footprint sites identified in lab strains to the natural populations Are the CRM sequences in the reference genome different from those in the Raleigh, N.C. sample? A concern was raised that laboratory lines might be subject to conditions that may have led to the loss of ...
What Is Gene cloning and How Is It Used? 1. Explain what is meant
What Is Gene cloning and How Is It Used? 1. Explain what is meant

... What Is Gene cloning and How Is It Used? 1. Explain what is meant by the term "gene cloning" and indicate the main goals of this procedure. ...
What Is Gene cloning and How Is It Used? 1. Explain what is meant
What Is Gene cloning and How Is It Used? 1. Explain what is meant

... What Is Gene cloning and How Is It Used? 1. Explain what is meant by the term "gene cloning" and indicate the main goals of this procedure. ...
Chapter 15 – Recombinant DNA and Genetic Engineering
Chapter 15 – Recombinant DNA and Genetic Engineering

... homologous DNA sequences resulting in fragments of different lengths are restriction fragment length polymorphisms (RFLP’s) ...
What is a genome?
What is a genome?

... human genome 1953. Watson and Crick propose the double helix model for DNA 1977. Sanger proposes the sequencing method with terminators 1986. Dulbecco auspicates in Science sequencing of the human genome 1988. Watson becomes director of the project at the NIH 1991. Craig Venter (then NIH) publishes ...
No Slide Title
No Slide Title

... the Gene(s) to Study • How do we integrate all the available information that we and others generate? • How do we locate the one or few genetic variations involved in our trait in the sea of hundreds or thousands of possible variations? • Most methods identify a set, often a large set, of possible v ...
Genetica per Scienze Naturali aa 05
Genetica per Scienze Naturali aa 05

... In the latter case, nearly all sequence positions are the same simply because not enough time has elapsed since the last common ancestor for large numbers of changes to have occurred. In contrast, because of functional constraints in human-mouse comparisons the exons in genes stand out as small isla ...
Mutation or polymorphism?
Mutation or polymorphism?

... mutation changes this to a rare and abnormal variant. In contrast, a polymorphism is a DNA sequence variation that is common in the population. In this case no single allele is regarded as the standard sequence. Instead there are two or more equally acceptable alternatives. The arbitrary cut-off poi ...
Multiple choice questions
Multiple choice questions

... are not very common in the human genome can be used in genetic mapping of genomes are usually longer than 200 bp are normally found at the end of chromosomes Transcriptomes consist of RNA consist of DNA consist of proteins are translated into proteins do not change differ in different cells ...
Principle of TAIL-PCR
Principle of TAIL-PCR

... Many product bands from the primary TAIL-PCR reaction disappeared after the secondary TAIL-PCR, indicating that these were non-specific type II products Specific products were not always seen in the primary reactions due to their low concentration. However, these specific products becomes visible af ...
Data/hora: 08/05/2017 02:33:57 Biblioteca(s): Embrapa Meio
Data/hora: 08/05/2017 02:33:57 Biblioteca(s): Embrapa Meio

... impact of intensive agriculture on these systems. Studies have identified an area of high risk of groundwater contamination by agrochemicals, since geological formation of the region exposes a natural vulnerability of an aquifer just below (100m), associated to the intense use of agrochemicals highl ...
What is bioinformatics? - The British Association of Sport and
What is bioinformatics? - The British Association of Sport and

... The genomes for many prokaryote, eukaryote, plant, invertebrate and vertebrate model species have now been sequenced. The DNA sequences of these genomes have been posted online. However, these websites contain much more than just the “naked” DNA sequence which has limited use. With the help of speci ...
Browsing the Genome
Browsing the Genome

... ESTs, mRNAs, CpG islands, assembly gaps and coverage, chromosomal bands, mouse homologies, and more Annotation tracks are both computed at UCSC from publicly available sequence data and provided by collaborators Users can also add their own custom tracks to the browser ...
Molecular Evidence for Evolution
Molecular Evidence for Evolution

... Chimpanzees and humans turn out to be very similar—if you look at their DNA. When scientists determined the entire genetic code of both humans and chimpanzees, they found that we have over 98% identical DNA. Molecular Evidence ...
슬라이드 1
슬라이드 1

... events resulting in a widespread distribution of complete or partial retroviral sequences throughout the human genome. The human genome comprises approximately 8% of the human endogenous retroviruses (HERVs) and other long terminal repeat (LTR)–like elements. Most HERVs seem to have entered the geno ...
Designer science and the “omic” revolution
Designer science and the “omic” revolution

... and transport processes through cellular membranes at given times. We might expect ...
Oncomine - OpenWetWare
Oncomine - OpenWetWare

... Author_type of cancer Description of Cancer ...
Genomics - University of Missouri
Genomics - University of Missouri

... Genes compromise a very small percentage of the DNA that makes up the chromosome, <5% generally. DNA containing genes is called euchromatin. ...
Supplementary material 1 grimalt
Supplementary material 1 grimalt

... Reagent (Gibco, Paisley, UK) using Eppendorf-fitting, RNase free pestles (Iberlabo, Madrid, Spain). RNA was extracted in TRIzol as specified by the supplier. Total RNA concentration was estimated by spectrophotometric absorption at 260 nm in a Nanodrop Spectrophotometer ND-1000 (NanoDrop Technologie ...
Jeffreys - OldForensics 2012-2013
Jeffreys - OldForensics 2012-2013

... first developed DNA fingerprinting techniques those of which are commonly used today for police and detective work, paternity tests, and immigration issues ...
GOBASE—a database of organelle and bacterial
GOBASE—a database of organelle and bacterial

... of complex genes (Figure 1a) and neighbouring genes on the chromosome (Figure 1b). This also allows for a more sophisticated representation of trans-spliced genes than has previously been possible. Information from the Gene Ontology project (9) has also been integrated into the GOBASE database. Ever ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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