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... collection of genes an organism has.  Ex: Human Genome Project- scientists now know the sequence of 20,500 genes!  Gene technology helps scientists study genomes of organisms ...
III. Biotechnology
III. Biotechnology

... Biotechnology ...
File - Mr. Lambdin`s Biology
File - Mr. Lambdin`s Biology

... = PCR – A Polymerase Chain Reaction machine makes copies of the sequences that you want ...
Chapter 13 – Genetic Engineering
Chapter 13 – Genetic Engineering

... – Ex – bananas, citrus fruit, strawberries, many ornamental flowers Diploid corn Tetraploid corn ...
Chapter 13 – Genetic Engineering
Chapter 13 – Genetic Engineering

... – Ex – bananas, citrus fruit, strawberries, many ornamental flowers Diploid corn Tetraploid corn ...
U.S. Meat Animal Research Center (MARC)
U.S. Meat Animal Research Center (MARC)

... Current limitations in genomic research Single gene selection is worse than single trait selection  Need to incorporate with EPDs We need to rapidly identify sufficient number of genes that explain the majority of the genetic variation  Need for additional laboratory tools ...
Chapter 13 – Genetic Engineering
Chapter 13 – Genetic Engineering

... Producing new kinds of bacteria • Can expose millions of bacteria at one time to radiation – increases chances of producing a successful mutant. – Ex – bacteria that can digest oil have been produced this way ...
Seeking Out Dislipidemia Variants with LipidSeq
Seeking Out Dislipidemia Variants with LipidSeq

... samples, and receive good quality data in return. For example, we work with the staff of the LRGC to set up experiments that meet their customers’ needs. They like that fact that the MiSeq System can sequence small bacterial genomes and transcriptomes, that they can multiplex samples on 1 run cartri ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... and 1173 to 1194, respectively. The PCR amplifications of nodD and nifH genes were carried out with the DNA extracted from the same isolated colonies using the primer pair 5⬘-CTCGTCGCGCTCGACGCATTGA-3⬘ and 5⬘-TGCCCCAT GGACATGTA-3⬘ (nodD) and the primer pair 5⬘-GTCTCCTATGACGTGCT CGG-3⬘ and 5⬘-GCTTCCAT ...
slides
slides

... Mutations in bacteria can be selected by a change in the environment. Grown in the presence of rifampicin, mutants with an RNA polymerase that is not disabled by this antibiotic survive and take over the culture. In any large population of bacteria there will be many different “mutants” (genetic va ...
Bi 430 / 530 Theory of Recombinant DNA Techniques Syllabus
Bi 430 / 530 Theory of Recombinant DNA Techniques Syllabus

... Why is PCR such a versatile tool for nucleic acid studies? What DNA sequences exist in nature, and what are they for? How are computers used to manage and study biological information? How can all of the genes in a genome be studied at once? How is DNA moved into and between biological systems? ...
DNA barcoding as a diagnostic tool DNA barcoding is a generic
DNA barcoding as a diagnostic tool DNA barcoding is a generic

... Overall diagnostic sensitivity was 87%. Analysis of the TPS data showed that neither the amplification/sequencing of loci, nor the creation of consensus sequences, negatively influenced the diagnostic sensitivity. Consensus sequence data generated by the participants was highly similar to the refere ...
LINEs
LINEs

... Transposons can be used to transfer DNA between bacterial cells Transposons (pink) integrate into new sites on the chromosome or plasmids by non-homologous recombination. Integrons (dark green) use similar mechanisms to exchange single gene cassettes (brown). ...
BioCyc Microbial Genomes and Metabolic Pathways Web Portal
BioCyc Microbial Genomes and Metabolic Pathways Web Portal

... times per year, often with new databases present. Two members of the BioCyc collection, the EcoCyc [1] and MetaCyc [2] databases, are derived from more than three decades of literature-based curation of genome and pathway data. The HumanCyc database provides a curated collection of many human metabo ...
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Heat, salt, pressure, acidity - how `extremophile` bacteria are yielding

... This may be because standard culture media – a kind of nutrient-rich jelly on which bacteria can be grown in a lab – does not contain all the other microscopic organisms that live in the natural deep-sea environment. It seems that extreme marine bacteria rely partly on the by-products of metabolism ...
Ch 7 Control of Microbial Growth
Ch 7 Control of Microbial Growth

... (TDT): Time to kill all cells in a culture • Decimal reduction time (DRT): Minutes to kill 90% of a population at a given temperature ...
Genome - Faperta UGM
Genome - Faperta UGM

... The size of genomes is given in base pairs (bp) The size of genomes is species dependent The difference in the size of genome is mainly due to a different number of identical sequence of various size arranged in sequence The gene for ribosomal RNAs occur as repetitive sequence and together with the ...
Using GenomiPhi DNA Amplification Kit for the Representative
Using GenomiPhi DNA Amplification Kit for the Representative

... enzyme with excellent strand displacement activity, along with random-sequence hexamer primers to amplify DNA. Most notable features include simplicity of use, high sensitivity, preservation of genetic heterogeneity, and easy compatibility of GenomiPhi products with downstream applications such as P ...
bc lecture 1
bc lecture 1

... chromosome 22 and is thought to be a so-called "tumor suppressor gene." Metadata (from an online publication database) The pattern of inheritance is autosomal dominant, caused by a spontaneous mutation in the egg or sperm before fertilization. Visualization of NF2 Gene on ...
Caenorhabditis Annotation in UniProtKB
Caenorhabditis Annotation in UniProtKB

... UniProtKB works closely with both the nematode worm research community and with WormBase, the database of the biology and genome of C. elegans and related nematode species, to ensure that UniProtKB presents detailed and current proteomes, sequences and functional annotation of nematode proteins in a ...
Diapositive 1
Diapositive 1

... If we want to be 100% sure of the assignment of a taxon, then we must look at the nodes below the closest node excluding a sister group with probability p < 0.01. Below this point, a series of statistical and classificatory approaches allow us to estimate the probability that the query sequence belo ...
Ph.D. Human Genetics - Central University of Punjab
Ph.D. Human Genetics - Central University of Punjab

... and eukaryotic systems; Expression of recombinant proteins using bacterial, animal and plant vectors; Isolation of specific nucleic acid sequences, Generation of genomic and cDNA libraries in plasmid, phage, cosmid, BAC and YAC vectors; In vitro mutagenesis and deletin techniques, gene knock out in ...
Ch 12 Gen Eng QA PP Ques 1
Ch 12 Gen Eng QA PP Ques 1

... Result is a recombinant plasmid which, when inserted into a bacterial cell, will multiply the new DNA (clone) (steps 5-6) Note: the plasmid vector usually also contains an antibiotic resistance gene that will allow scientists to isolate colonies that have the GOI. (Will grow bacteria on pates w/anti ...
EXPRESSED SEQUENCE TAGS FROM IMMUNE TISSUES OF
EXPRESSED SEQUENCE TAGS FROM IMMUNE TISSUES OF

... immune-relevant tissues (liver, spleen and head kidney) were sampled at several time points in the infection process for library construction. The sequences were processed into 25,644 high-quality sequences, which constituted the source for the olive flounder EST database. Clustering and assembly of ...
4mb ppt
4mb ppt

... chromosomes and telomeres where they are thought to participate in the structure of these specialized regions of chromosomes. ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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