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goals - s3.amazonaws.com
goals - s3.amazonaws.com

... shows the presence(+) or absence (-) of the “Alu” DNA sequence on the paternal and maternal chromosomes. • We will use primers for the Alu sequence that will generate a 550 bp fragment if the Alu sequence is not present and an 850 bp fragment if the Alu sequence is present through the application of ...
Molecular_Genetic_Characterization[1]
Molecular_Genetic_Characterization[1]

... identified in strawberry. Current efforts under this award will test to see if these sequences may complement Arabidopsis mutants, and they also will be overexpressed in the ‘Laboratory Festival #9’ background. The blots that have been prepared will be probed to describe expression patterns to deter ...
glossary - Diabetes Care
glossary - Diabetes Care

... evolutionarily conserved pathways associated with microbial pathogenesis. In particular, this has revealed important factors of the host response with remarkable parallels in higher organisms. This organism’s short (2–3-weeks) life span facilitates host-bacteria interaction analysis, offering an ide ...
Powerpoint template for scientific posters (Swarthmore
Powerpoint template for scientific posters (Swarthmore

... In this project, 11 students from two of the collaborating institutions contributed to this inaugural research experience, which included both computer-based annotation and benchtop components. The following questions were asked: 1. Is there evidence to support the original functional prediction(s) ...
The Human Genome Project
The Human Genome Project

... The government has a database of real fingerprints. Should the government have a data base of DNA fingerprints? What is the difference? Should they collect DNA from everyone? ...
Chapter 1 - s3.amazonaws.com
Chapter 1 - s3.amazonaws.com

... Eukaryotic Cells Eukaryotic cells -possess a membrane-bound nucleus -are more complex than prokaryotic cells -compartmentalize many cellular functions within organelles and the endomembrane system -possess a cytoskeleton for support and to maintain cellular structure ...
comparing dna sequences to determine evolutionary relationships
comparing dna sequences to determine evolutionary relationships

... the same species, while there is significant variation in COI sequences of organisms from different species. Therefore, a COI sequence provides a unique sequence signature for a particular species. For the same reasons, the COI gene is suitable for comparing phylogenetic relationships between specie ...
The_Human_Microbiome_Congress_
The_Human_Microbiome_Congress_

... Adaptation of the human gut microbiome to its unique environment is typically described in terms of the relative composition and diversity of microbial species. An alternative (and complementary) perspective can provided by analyzing the relative distribution of protein families in metagenomic datas ...
Phenotype
Phenotype

... • Mechanistically predicting relationships between different data types is very difficult • Empirical mappings are important • Functions from Genome to Phenotype stands out in importance G is the most abundant data form - heritable and precise. F is of greatest interest. DNA ...
A History of Genetics and Genomics
A History of Genetics and Genomics

... The information age is essential to genomics. The electronic analysis, distribution and storage of genomic data is a hallmark of the science. Critical to this was the development of computers, both large and small, which put computing power in the hands of all scientists. The free distribution of an ...
Intelligence Advanced Research Projects Activity - NAS
Intelligence Advanced Research Projects Activity - NAS

... • Improved capability to address unknown threats • Computational models that inform new/emerging threats • Systems: bacterial, toxin, viral, host response, etc. • Test data provided by T&E performer ...
restriction enzymes
restriction enzymes

... •Gene cloning = replication of a target sequence of DNA •insert target sequence into an easily replicated vector •insert the vector into a single bacteria (transformation) •allow the bacteria to amplify •vector has sequences that enable coordinated replication of the recombinant vector DNA •DNA Clon ...
Practical Lecture 1
Practical Lecture 1

... • Detailed genetic information: • gene names • phenotype of mutants • location of genes/mutations on chromosomes • linkage (distances between genes) • High Throughput lab technologies • PCR • Rapid inexpensive DNA sequencing (Illumina HiSeq) • Microarrays (Affymetrix) • Genome-wide SNP chips / SNP a ...
7.012 Problem Set 7 FRIDAY December 3, 2004 Not due unless you
7.012 Problem Set 7 FRIDAY December 3, 2004 Not due unless you

... common diseases, such as diabetes, are the result of many affected genes. Comparing the variation between a group of diabetic individuals and a group of non-diabetic individuals may reveal regions of the genome that are different between these groups and may help to reveal the many genetic changes u ...
450 Mbp genome of rice, Oryza sativa
450 Mbp genome of rice, Oryza sativa

... 2. An enormous effort has gone into sequencing the maize genome, but at ~2.5 Gbp it is nearly the same size as ours, except even more of it is transposons, and these transposons form long stretches of seemingly barren genomic landscape. Like rice, but even more so, genes are islands in this sea of ...
Reading assessment for Unit 2
Reading assessment for Unit 2

... c. to pinpoint all of the cis-regulatory sequences for all genes in one experiment d. distinguish between transcription rate and RNA stability 2. Oligonucleotide arrays made by Affymetrix a. allow direct comparisons of two samples on a single array b. have high chip-to-chip reproducibility c. allow ...
Comprehensive Analysis of Antibiotic Resistance in
Comprehensive Analysis of Antibiotic Resistance in

... (http://www.lahey.org/Studies/). All genes of betalactamases are manually curated for the coding sequences with start codon and stop codon if those exist. The database was tested using whole genome sequence (WGS) data assembled from Illumina MiSeq data generated on eight species of clinical isolates ...
my_phylogeny1
my_phylogeny1

... Two homologous DNA sequences which descended from an ancestral sequence and accumulated mutations since their divergence from each other. Note that although 12 mutations have accumulated, differences can be detected at only three nucleotide sites. ...
amazing facts about human dna and genome
amazing facts about human dna and genome

... DNA that is mostly non-coding. Although the best estimates are probably around 30,000 to 40,000 genes, analysis of the same human genome sequence has resulted in estimates of from 25,000 to 70,000 genes. Many predicted genes could be inactive pseudo genes and conversely, many genes may be overlooked ...
"Evolution and function of eukaryotic-like proteins in
"Evolution and function of eukaryotic-like proteins in

... 6th November 2013, Fremantle - Australia ...
Montel
Montel

... Frequent fails due to gap between the results obtained in vitro and those obtained in cheeses  Consortia with high antilisteria activities by in vitro test but not at the surface of cheeses Low number of strains selected for example in Truefood project able to inhibit and without effect on sensor ...
Genetic Analysis and Mapping in Bacteria and Bacteriophages
Genetic Analysis and Mapping in Bacteria and Bacteriophages

...  Since bacteriophages kill their host bacteria, we can count the number of phage in a sample by doing a plaque assay  Each plaque represents an individual phage particle  Phage genes can be mapped by recombination frequencies in a manner similar to eukaryotic gene mapping Viral life cycle  Tempe ...
Answers - Easy Peasy All-in
Answers - Easy Peasy All-in

... 6. Mendel’s second set of experiments showed that two different traits were inherited by the offspring independently of each other. The second generation of offspring had all possible combinations of the two traits. Mendel inferred from these results that the factors controlling different traits ass ...
A History of Genetics and Genomics
A History of Genetics and Genomics

... The information age is essential to genomics. The electronic analysis, distribution and storage of genomic data is a hallmark of the science. Critical to this was the development of computers, both large and small, which put computing power in the hands of all scientists. The free distribution of an ...
BRAF: from gene to cancer therapy
BRAF: from gene to cancer therapy

... 4. In the Mutation Analysis box on the right of the screen, click on the Histogram button to see a graphical representation of the known mutations in the BRAF gene. Enter the amino acid range from the BRAF gene banner (581-621) into the boxes to the right of the histogram and press the red Apply b ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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