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DNA Technology
DNA Technology

... The chemical structure of everyone's DNA is the same. The only difference between people (or any animal) is the order of the base pairs. Using these sequences, every person could be identified solely by the sequence of their base pairs. However, because there are so many millions of base pairs, the ...
slides - Yin Lab @ NIU
slides - Yin Lab @ NIU

... This information is shown in “tracks,” with each track showing either the genomic sequence from a particular species or a particular kind of annotation on the gene. The tracks are aligned so that the information about a particular base in the sequence is lined up and can be viewed easily. In modern ...
Real-time PCR
Real-time PCR

... Less commonly used now Fluorophore ...
DNA barcoding: how it complements taxonomy, molecular
DNA barcoding: how it complements taxonomy, molecular

... photographs and locality information, and assembling the barcode library (i.e. a 650-base segment of the COI gene) [16]. The analysis of DNA barcoding data is usually performed by a clustering method, such as distance-based neighbor-joining (NJ) [25], and by evaluating genetic distances within and b ...
Document
Document

... their host phyla than their own (e.g. the difference in BLAST score, through phylogenetic tree building, and by identifying unusual codon usage). Is the gene or gene's pathway a usual component of the pathogens phyla? Also rank based on other factors such whether the candidate gene encodes a probabl ...
14-3 Human Molecular Genetics
14-3 Human Molecular Genetics

... 14-3 Human Molecular Genetics Genetics ...
No Slide Title
No Slide Title

... •Others libraries may have gene in lower abundance, but they are specific to that library. Its possible to find the specific genes by ‘subtracting’ the sequences present in other tissues. E.g. A library from pathogen-infected tissue with the sequences from uninfected tissue subtracted out. •Expressi ...
ch 17 - Quia
ch 17 - Quia

... -Ability to create mutations at any site in a cloned gene -Has been used to produce knockout mice, in which a known gene is inactivated -The effect of loss of this function is then assessed on the entire organism -An example of reverse genetics ...
Chapter 13: DNA Technology
Chapter 13: DNA Technology

... Chapter 13: DNA Technology ...
Gene Mapping Techniques - Nestlé Nutrition Institute
Gene Mapping Techniques - Nestlé Nutrition Institute

... ing common ancestors. In the mouse the use of congenic strains, and especially recombinant inbred strains (RIS), has proved very useful. The use of RIS for the detection of linkage has been extensively reviewed by Taylor (4), who has considered each strain as the equivalent of an F2 individual with ...
B.Sc. (Microbiology)
B.Sc. (Microbiology)

... Cell-cell interaction, receptors and models of extra-cellular signaling: cytosolic, nuclear and membrane bound receptors, autocrine, paracrine and endocrine model of action. Muscles and nerve cells, cell senescence and death, cell differentiation. Signal transduction: signal amplification and differ ...
We present here a collection of DNA sequence
We present here a collection of DNA sequence

... from mainframe computers and DNA sequence databases. INTRODUCTION Although it has only been two years since the first issue of Nucleic Acids Research devoted solely to the use of computers in DNA sequence analysis (Vol. 10, No. 1 (1982)), there have been several changes in the field. First, the numb ...
PCR reading answers
PCR reading answers

... off List two examples indicating why this info is useful ? In general, this info helps us compare abnormal and normal tissue (i.e. cancer vs. healthy). This info can also be helpful in determining how cells will respond to different drugs. 23. In step one, what is each dot on a printed microarray ? ...
14–3 Human Molecular Genetics
14–3 Human Molecular Genetics

... There are roughly 6 billion base pairs in your DNA. Biologists search the human genome using sequences of DNA bases. ...
The Microarray Platform of IVM/IZKF
The Microarray Platform of IVM/IZKF

... availability of robust hardware- and software platforms to produce and evaluate microarrays have enabled genome-wide gene expression analyses, i.e. to quantify all mRNAs (> 30 000) of a total RNA extract relative to another RNA extract, within 48 hours. The platform used by the IVM (Affymetrix) is e ...
Resistance gene naming and numbering: is it a
Resistance gene naming and numbering: is it a

... larger difference at the DNA level, this value seems far too large. On the other hand, a single base pair difference seems far too small, as reported differences have previously been traced to sequencing errors. In the interests of moving this debate into the public arena, we propose that a threshol ...
Lecture 27
Lecture 27

... Test next Friday ...
Lab5CytochromeC
Lab5CytochromeC

... the amino acids are always the same in all species. These have been shaded light gray. Be sure to study the entire molecule. Each protein sequence has 103- 112 amino acids; the sequence extends onto two (2) lines. Differences: Tuna and Fly _______ In the example below there are 9 differences ...
Lab 12
Lab 12

... sequences -each enzyme recognizes and cuts DNA at a different base sequence e.g. BamHI XXXXXXXXGGATCCXXXXXXXXXX XXXXXXXXCCTAGGXXXXXXXXXX -due to spontaneous mutations over time, different people have slightly different base sequences in their DNA -if mutation creates or deletes a restriction site in ...
A Variable Number of Tandem Repeats Locus with!, the Human
A Variable Number of Tandem Repeats Locus with!, the Human

... separate unequal crossing-over events involving the 5' conserved core (Fig. 3). The three observed deletions/insertions probably represent three distinct genetic events. Each of these events should have resulted in two reciprocal alleles leading to a larger total number of alleles than the reported ...
Comparative Genomic Study of upstream Open Reading Frames
Comparative Genomic Study of upstream Open Reading Frames

... Identification of elements responsible for posttranscriptional control represents one of the biggest problems in modern biology. Real challenge is to find those conserved motifs and characterize them in order to make a computational tool, which will be able to scan entire genome and distinguish elem ...
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine

... Genomic Library for isolating a specific gene ± has non-coding regions in it x cDNA is just the coding region and will have introns removed to generate protein x Vector: DNA to be inserted is small (6-10kb) o ȜSKDJHYHFWRUV can handle up to 20kb ± infects E.coli o COSmids: can handle up to 50kb ƒ P ...
+ – DNA
+ – DNA

... be done now that we know the order (sequence) in which all 3.2 billion bases occur? ...
CBOL Protist Working Group: Barcoding Eukaryotic
CBOL Protist Working Group: Barcoding Eukaryotic

... the group. The genetic divergence observed between and within major protistan groups greatly exceeds that found in each of the three multicellular kingdoms. No single set of molecular markers has been identified that will work in all lineages, but an international working group is now close to a sol ...
PharmGKB - SNP Use Case
PharmGKB - SNP Use Case

... 2. A list of all SNPs in all the nuclear genes that interact with mitochondrial genes with their known functional implications, correlations to known disease, and any known effects on drugs or adverse drug reactions. 3. A comparison of all the SNP allele frequencies across all study groups (especial ...
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Metagenomics



Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.
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