DNA cloning by homologous recombination in Escherichia coli
... Redα/Redβ, and the absence of amplification of the plasmid origin/antibiotic resistance gene cassettes. (B). Summary of five examples of the reaction diagrammed in (A). The linear cloning vector column states the plasmid origin/selectable RecBCD, homologous recombi- marker cassette joined, by PCR, t ...
... Redα/Redβ, and the absence of amplification of the plasmid origin/antibiotic resistance gene cassettes. (B). Summary of five examples of the reaction diagrammed in (A). The linear cloning vector column states the plasmid origin/selectable RecBCD, homologous recombi- marker cassette joined, by PCR, t ...
WALKTHROUGH FOR ANALYSIS OF CODON PAIRING USING
... The program codoncount.txt counts all the codon pairs in a genome ORF by ORF and records the total observed count of each of the 3904 pair types (61 sense codons x 64 sense and stop codons). It also calculates the expected number of codon pairs as each ORF is processed, and records a cumulative expe ...
... The program codoncount.txt counts all the codon pairs in a genome ORF by ORF and records the total observed count of each of the 3904 pair types (61 sense codons x 64 sense and stop codons). It also calculates the expected number of codon pairs as each ORF is processed, and records a cumulative expe ...
Forensic ABO blood grouping by 4 SNPs analyses using an ABI
... 2-1-1Hongo bunkyo-ku, Tokyo 113-8421, Japan Medico-Legal Section, Criminal Investigation Laboratory, Metropolitan Police Department, Tokyo, Japan ...
... 2-1-1Hongo bunkyo-ku, Tokyo 113-8421, Japan Medico-Legal Section, Criminal Investigation Laboratory, Metropolitan Police Department, Tokyo, Japan ...
![nature | methods Versatile P[acman] BAC libraries for transgenesis](http://s1.studyres.com/store/data/016819563_1-9796374085bf2dc123930427a979e4a9-300x300.png)
nature | methods Versatile P[acman] BAC libraries for transgenesis
... DNA, isolated from the isogenized y1; cn1 bw1 sp1 strain, was partially digested with MboI and ligated into the BamHI site of the attB-P(acman)-CmR-BW plasmid. White recombinant clones ...
... DNA, isolated from the isogenized y1; cn1 bw1 sp1 strain, was partially digested with MboI and ligated into the BamHI site of the attB-P(acman)-CmR-BW plasmid. White recombinant clones ...
Evolution of colour vision in primates
... MWS gene. Their arrangement and proximity suggests that they have arisen by gene duplication. In this process, a gene is duplicated and one of the copies accumulates mutations and can become a ‘new’ gene encoding a protein with a different function. About 40% of the genes in the human genome may hav ...
... MWS gene. Their arrangement and proximity suggests that they have arisen by gene duplication. In this process, a gene is duplicated and one of the copies accumulates mutations and can become a ‘new’ gene encoding a protein with a different function. About 40% of the genes in the human genome may hav ...
IOSR Journal of Environmental Science, Toxicology and Food Technology (IOSR-JESTFT)
... Various strains of Lysinibacillus fusiformis have been widely employed in biodegradation of environmental contaminants such as hydrocarbon [25], sulfonated azo dyes [26], etc. Lysinibacillus sp. isolated from agricultural land has biodegrading activity on herbicide fomesafen and could degrade upto 8 ...
... Various strains of Lysinibacillus fusiformis have been widely employed in biodegradation of environmental contaminants such as hydrocarbon [25], sulfonated azo dyes [26], etc. Lysinibacillus sp. isolated from agricultural land has biodegrading activity on herbicide fomesafen and could degrade upto 8 ...
A Hybrid Knowledge-Driver Approach to Clustering Gene
... The main limitation of many gene expression analytic approaches is the fact that they do not successfully incorporate domain knowledge about the genes into the actual process, compromising the quality of the results obtained. Once the clustering algorithm has terminated, the challenge is to validate ...
... The main limitation of many gene expression analytic approaches is the fact that they do not successfully incorporate domain knowledge about the genes into the actual process, compromising the quality of the results obtained. Once the clustering algorithm has terminated, the challenge is to validate ...
Chromosomal changes associated with changes in development
... glycoprotein genes in Trypanosoma brucei. At the top is a restriction map of the basic copy of the VSG gene 118. In each independently isolated clone of trypanosome expressing the 118 VSG an additional copy can be detected - the ELC. Four such examples are shown. Comparison of the maps show that eac ...
... glycoprotein genes in Trypanosoma brucei. At the top is a restriction map of the basic copy of the VSG gene 118. In each independently isolated clone of trypanosome expressing the 118 VSG an additional copy can be detected - the ELC. Four such examples are shown. Comparison of the maps show that eac ...
TO: Beth Dobkin, Provost FROM: Hisham Ahmed, Chair Academic
... Understanding Learning Goal. To make it more appealing to all students, we would like to be able to use either “Introductory Microbiology” and/or “Microbes and Society” With the increasing knowledge regarding the importance of the microbiome for our well being and importance of microbes in the biore ...
... Understanding Learning Goal. To make it more appealing to all students, we would like to be able to use either “Introductory Microbiology” and/or “Microbes and Society” With the increasing knowledge regarding the importance of the microbiome for our well being and importance of microbes in the biore ...
Probing Essential Nucleobase Functional Groups in Aptamers and
... DNA (dNAIM) that is based on solid-phase synthesis of nucleotide analogue libraries (Figure 1a). In contrast to the phosphorothioate tag in NAIM analysis, we use the 20 -OH group of ribonucleotides as a chemical tag to encode nucleotide analogues in combinatorial DNA libraries. This is achieved by s ...
... DNA (dNAIM) that is based on solid-phase synthesis of nucleotide analogue libraries (Figure 1a). In contrast to the phosphorothioate tag in NAIM analysis, we use the 20 -OH group of ribonucleotides as a chemical tag to encode nucleotide analogues in combinatorial DNA libraries. This is achieved by s ...
LightCycler® 480 System - Gene Scanning
... in PCR amplicons. It goes beyond the power of classical melting curve analysis by allowing to study the thermal denaturation of a double-stranded DNA in much more detail and with much higher information yield than before. One key application of HRM is gene scanning - the search for the presence of u ...
... in PCR amplicons. It goes beyond the power of classical melting curve analysis by allowing to study the thermal denaturation of a double-stranded DNA in much more detail and with much higher information yield than before. One key application of HRM is gene scanning - the search for the presence of u ...
Introduction to Bioinformatics
... The purpose of this exercise is to introduce to you and familiarize you with the National Center for Biotechnology Information (NCBI) web sites. These web sites contain nucleotide and protein databases containing a vast amount of sequencing data that is increasing every single day. As more and more ...
... The purpose of this exercise is to introduce to you and familiarize you with the National Center for Biotechnology Information (NCBI) web sites. These web sites contain nucleotide and protein databases containing a vast amount of sequencing data that is increasing every single day. As more and more ...
Impact of Salt Concentration on Electricity Production in
... novel purpose of electricity generation in the process of waste water treatment. Hence, it is an ideal solution for sustainable non renewable source of energy. MFC can be best defined as a fuel cell where microbes act as catalyst in degrading the organic content to generate electricity. MFCs operate ...
... novel purpose of electricity generation in the process of waste water treatment. Hence, it is an ideal solution for sustainable non renewable source of energy. MFC can be best defined as a fuel cell where microbes act as catalyst in degrading the organic content to generate electricity. MFCs operate ...
Document
... from a single gene multiple transcripts) AS is tissue-specific (Graveley, 2001) AS is related to human diseases ...
... from a single gene multiple transcripts) AS is tissue-specific (Graveley, 2001) AS is related to human diseases ...
The vertebrate genome annotation (Vega) database
... and zebrafish, are being fully sequenced and finished to a quality which merits manual annotation. Although labour intensive and relatively slow compared with automatic annotation methods, manual annotation provides an invaluable reliable reference resource that can be used to predict gene structures ...
... and zebrafish, are being fully sequenced and finished to a quality which merits manual annotation. Although labour intensive and relatively slow compared with automatic annotation methods, manual annotation provides an invaluable reliable reference resource that can be used to predict gene structures ...
![[ 19] Saccharomyces Genome Database - SGD-Wiki](http://s1.studyres.com/store/data/012906934_1-a4c322e0bc64d409034f1882ecf44124-300x300.png)
[ 19] Saccharomyces Genome Database - SGD-Wiki
... Web site. This form allows users to create or update their contact information in SGD, thus providing other members of the research community with telephone and mail information, Web page addresses, a description of research interests, lists of co-workers, and other relevant data. E-mail to Curators ...
... Web site. This form allows users to create or update their contact information in SGD, thus providing other members of the research community with telephone and mail information, Web page addresses, a description of research interests, lists of co-workers, and other relevant data. E-mail to Curators ...
METABOLIC MODELING AND IN SILICO METABOLISM
... There is no direct relationship between metabolite and gene in the way there is between genes and mRNAs and proteins. A single gene does not specify the level of a single metabolite, i.e. its pool size (although it may determine whether the metabolite is present or absent). Rather, as MCA teaches, t ...
... There is no direct relationship between metabolite and gene in the way there is between genes and mRNAs and proteins. A single gene does not specify the level of a single metabolite, i.e. its pool size (although it may determine whether the metabolite is present or absent). Rather, as MCA teaches, t ...
Saccharomyces Genome Database.
... Web site. This form allows users to create or update their contact information in SGD, thus providing other members of the research community with telephone and mail information, Web page addresses, a description of research interests, lists of co-workers, and other relevant data. E-mail to Curators ...
... Web site. This form allows users to create or update their contact information in SGD, thus providing other members of the research community with telephone and mail information, Web page addresses, a description of research interests, lists of co-workers, and other relevant data. E-mail to Curators ...
Mutations in a gene encoding a novel protein tyrosine
... identity with transcript A, except for the omission of a 1,770-bp segment due to splicing (Figs 3, 4). The common origin of transcripts A and B suggests they are alternative forms of the same gene, the products of which would be predicted to have unique carboxy-terminal amino acid sequences (Fig. 4b ...
... identity with transcript A, except for the omission of a 1,770-bp segment due to splicing (Figs 3, 4). The common origin of transcripts A and B suggests they are alternative forms of the same gene, the products of which would be predicted to have unique carboxy-terminal amino acid sequences (Fig. 4b ...
annotation and analysis of newly discovered mycobacteriophage
... host. We are analyzing two novel mycobacteriophage, Firecracker and Dori, which were isolated on the UCSC campus using Mycobacterium Smegmatis as the viral host. After multiple rounds of plaque purification, we performed electron microscopy and observed that Dori has a typical siphoviral morphology ...
... host. We are analyzing two novel mycobacteriophage, Firecracker and Dori, which were isolated on the UCSC campus using Mycobacterium Smegmatis as the viral host. After multiple rounds of plaque purification, we performed electron microscopy and observed that Dori has a typical siphoviral morphology ...
Search - VectorBase
... or by the number of hits. Click on the plus sign to show or hide categories (available options depend on which Domain you have selected). ...
... or by the number of hits. Click on the plus sign to show or hide categories (available options depend on which Domain you have selected). ...
Molecular Evolutionary Analysis of Classical Swine Fever Virus
... have divided the viruses into three main groups, and indicated that all the isolates in mainland China belonged to Group 1 and 2. It is well known that the Group 1 viruses consist of highly virulent strains which could cause acute CSF, while the Group 2 viruses are characterized by moderately virule ...
... have divided the viruses into three main groups, and indicated that all the isolates in mainland China belonged to Group 1 and 2. It is well known that the Group 1 viruses consist of highly virulent strains which could cause acute CSF, while the Group 2 viruses are characterized by moderately virule ...
Article A Distinct Mitochondrial Genome with DUI
... Ais_16S_d4 haplotype from male BS animals deviated from the nucleotide substitution pattern shared by the other divergent haplotypes at position 11. Two “normal” haplotypes, Ais_16S_n4 and Ais_16S_n5 from female animals (from BS and NS, respectively), had a “divergent”-like substitution at positions ...
... Ais_16S_d4 haplotype from male BS animals deviated from the nucleotide substitution pattern shared by the other divergent haplotypes at position 11. Two “normal” haplotypes, Ais_16S_n4 and Ais_16S_n5 from female animals (from BS and NS, respectively), had a “divergent”-like substitution at positions ...
Partial Sequence Analysis of the 5S to 18S rRNA Gene Region of
... end, and show that homology varies greatly in different regions of the molecule. For example, from -300 to -180, the homology with E. coli is 81.7%. From -180 to -60, the homology is decreased to 62.2% excluding gaps and to 38.3% if gaps are included. The last 60 nucleotides have 86.7% homology. Var ...
... end, and show that homology varies greatly in different regions of the molecule. For example, from -300 to -180, the homology with E. coli is 81.7%. From -180 to -60, the homology is decreased to 62.2% excluding gaps and to 38.3% if gaps are included. The last 60 nucleotides have 86.7% homology. Var ...
Cluster analysis for microaray data
... expression patterns (e.g. cell cycle data). • Arrange a set of genes in a linear order that is at least not totally meaningless. ...
... expression patterns (e.g. cell cycle data). • Arrange a set of genes in a linear order that is at least not totally meaningless. ...
Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental samples. The broad field may also be referred to as environmental genomics, ecogenomics or community genomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Recent studies use either ""shotgun"" or PCR directed sequencing to get largely unbiased samples of all genes from all the members of the sampled communities. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale and detail than before.