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Genomics of the evolutionary process
Genomics of the evolutionary process

... sequence divergence really means. The genomics enterprise has fallen upon us so suddenly that it is hardly surprising that a thoughtful and contemplative field such as evolutionary biology is struggling to keep up. It is good for the field to have this upheaval, as it has brought in a wealth of new ...
Chapter Nineteen: Genomics
Chapter Nineteen: Genomics

... have diverged in function. 11. Describe several different methods for inferring the function of a gene by examining its DNA sequence. Homology: For protein-coding genes, the DNA sequence is translated conceptually into the amino acid sequence of the protein. The amino acid sequence of the protein th ...
Biotechnology - Explore Biology
Biotechnology - Explore Biology

... ~4 million base pairs  ~4300 genes  1/1000 DNA in eukaryote How have these little guys gotten to be so diverse?? ...
Tutorial 3 – Searching the Chinese Hamster
Tutorial 3 – Searching the Chinese Hamster

... The CHO-K1 genome database can be searched by Accession number (i.e. EGV99227) The database can be searched using the GenBank WGS protein accession IDs. For the CHO-K1 WGS project, these accession numbers are EGVXXXXX or EGWXXXXX. These accession numbers can also be found in the NCBI protein databas ...
Genetics Exam 5
Genetics Exam 5

... _____ A collection of plasmids containing foreign DNA fragments representing the entire genome of an organism is called a(n) A. transgenic ...
Genetic Diseases and Gene Therapy
Genetic Diseases and Gene Therapy

... • Integrate what you have learned about Genetic Engineering into your Gene Therapy Concept Map. • Gene Therapy Vector Engineering Project. ...
Biology 303 EXAM II 3/14/00 NAME
Biology 303 EXAM II 3/14/00 NAME

... The fact that there is a problem maintaining the very ends of eukaryotic chromosomes during replication has to do with 1. the fact that eukaryotic chromosomes are linear. 2. the inability of DNA polymerases to initiate synthesis without a primer. 3. the restriction that DNA synthesis must occur in a ...
Pathogen Genomics COURSE
Pathogen Genomics COURSE

... information (i.e. genes) or which project it is from. Download the text file (unknown_DNA.txt) of DNA sequence data from the course website (http://hyphy.org/w/index.php/MED263). 5.2) First use GLIMMER (http://www.ncbi.nlm.nih.gov/genomes/MICROBES/glimmer_3.cgi) at the NCBI to determine if the DNA s ...
DNA
DNA

... The Nuclear genome consists of DNA in the nucleus of eukaryotic cells – this is what we typically think of as our Genome: A Genome is the unique set of chromosomes (or DNA) in one cell of an organism. • Humans have 2 sets of chromosomes (one from each parent: we are diploid.) • Our genome consists ...
Document
Document

... •An Engineering technology based on biology which complements rather than replaces standard approaches •Engineering synthetic constructs will Enable quicker and easier experiments Enable deeper understanding of the basic mechanisms Enable applications in nanotechnology, medicine and agriculture ...
Cloning and Sequencing
Cloning and Sequencing

... when designing primers Actually not a single primer for each but a mixture of primers (oligoprimers) if the sequence of the target is not known If amino acid sequence of gene product is used then degenerate primers must be used Initial forward primer is GABTATGTTGTTGARTCTTCWGG B=G/T/C R=G/A (purines ...
BIOL 3300
BIOL 3300

... stages of mitosis and meiosis, extract DNA, transform bacteria, induce mutations with UV irradiation, use restriction enzymes, conduct electrophoresis, paper chromatography, and PCR, apply analytical methods using probabilities and Chi-square tests of significance. They will also analyze pedigrees, ...
Week 9 Pre-Lecture Slides
Week 9 Pre-Lecture Slides

... Would you expect to see more cancer in a population at war or at peace? Would you expect to see more cancer in a population with better or worse medicinal science? (This answer might be complicated…) ...
Improvement of GSH production by metabolic engineering the
Improvement of GSH production by metabolic engineering the

... • Glutathione (GSH) is a valuable tri-peptide that is widely used in the pharmaceutical, food, and cosmetic industries.Glutathione is produced industrially by fermentation using Saccharomyces cerevisiae. ...
Random-priming in vitro recombination: an effective tool for directed evolution ,
Random-priming in vitro recombination: an effective tool for directed evolution ,

... RPR technique may allow access to a greater range of amino acid substitutions than PCR-based point mutagenesis. RPR is a very flexible and easy to implement technique for generating mutant libraries for directed evolution. Other polymerases with different fidelities, including bacteriophage T4 DNA p ...
Recostructing the Evolutionary History of Complex Human Gene
Recostructing the Evolutionary History of Complex Human Gene

document
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... human body. The enzymes have evolved to function best at this temperature. 7) What is a restriction digest and what does it have to do with gel electrophoresis? A restriction digest is a chemical reaction where restriction enzymes cut up a strand of DNA into smaller pieces based on the number and lo ...
Transgenic Corn
Transgenic Corn

... Transgenic plants and genetically modified organisms are both created by selecting a specific gene or genes from one species and placing it into the DNA of another species. This results in a phenotypic change in the species that the gene(s) was implanted into. There are two methods for introducing ...
ppt - Sol Genomics Network
ppt - Sol Genomics Network

File - need help with revision notes?
File - need help with revision notes?

My Genetic Profile Worksheet
My Genetic Profile Worksheet

... • Each DNA cluster will be strongly attracted to any cDNA made from complimentary mRNA strands. For example: DNA strands with the base sequence TTCAGGCAG will be attracted to any cDNA strands with the sequence AAGTCCGTC. In other words each DNA cluster will be attracted to cDNA that were made using ...
TAKS Review - SchoolNotes
TAKS Review - SchoolNotes

... DNA makes an exact copy of itself using old strands as a template to make new strands. ...
Gene Technology Study Guide
Gene Technology Study Guide

... protein called green fluorescent protein (GFP) into various organisms. GFP, which is a substance naturally found in jellyfishes that live in the north Pacific Ocean, emits a green light when it its exposed to ultraviolet light. o Recombinant DNA - newly generated DNA molecule, with DNA from differen ...
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Slides

... known genes (although this percentage is of course increasing as more genomes get ...
File
File

... words, a change in a nucleotide pair may transform one codon into another that is translated into the same amino acid. Such a change is an example of a silent mutation, which has no observable effect on the phenotype. (Silent mutations can occur outside genes as well.) Substitutions that change one a ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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