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SNP Discovery by sequencing 1000 genomes
SNP Discovery by sequencing 1000 genomes

... entire genome -in SH3TC2 (the SH3 domain and tetratricopeptide repeats 2 gene) – cost ~$50,000 First to show how whole-genome sequencing can be used to identify the genetic cause of an individual's disease. "I have hundreds of thousands of differences from all the other genomes that have been sequen ...
Exam Review 2 - Fullfrontalanatomy.com
Exam Review 2 - Fullfrontalanatomy.com

... 26) The best definition of a true-breeding plant is one that ______. A) self-fertilizes to produce offspring identical to the parent B) becomes sterile after three generations C) produces sterile offspring when cross-fertilized D) self-fertilizes to produce hybrid offspring E) cannot be cross-fertil ...
During DNA replication, which of the following segments
During DNA replication, which of the following segments

... Similar enzymes interact with the leading and lagging strands in DNA replication. Enzymes involved with the RNA primer are more active on the lagging strand, as is the enzyme ____________________. ...
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine
Biochemistry ± DNA Chemistry and Analysis DNA o Adenosine

... x ¶SUHVHQWHGZLWK¶-hydroxyl on terminal nucleotide to remove form polynucleotide chain x ¶SUHVHQWHGZLWKIUHH¶-OH on terminal nucleotide to remove from polynuclotide chain ƒ Endonuclease: eat the interior RIDSRO\QXFOHRWLGHVSHFLILFIRU¶RU¶VLGHRISKRVphodiester bond x Can cut ssDN ...
Simple and straightforward construction of a mouse gene targeting
Simple and straightforward construction of a mouse gene targeting

... ligase. However, it is not always straightforward to find an appropriate unique recognition site within a given genomic segment. Even when a suitable restriction site exists, it still remains difficult to introduce only a single short loxP segment (40 bp) into a large plasmid (>10 kb) by convention ...
Horizontal Gene transfer
Horizontal Gene transfer

... A sizable fraction of bacterial genes are derived from horizontal gene transfer ...
Created with Sketch. Genetics webquest
Created with Sketch. Genetics webquest

Green Genomes - Columbia Blogs
Green Genomes - Columbia Blogs

... made piecing together sequenced DNA an even more daunting task. But the Arabidopsis DNA revealed that at least twice, the plant had somehow duplicated its entire genome and survived the unusual event. Subsequent genomes have revealed this to be a recurrent theme in plant evolution, with different sp ...
F: Acronyms and Glossary
F: Acronyms and Glossary

... living cell or organism. Introns: DNA sequences interrupting the protein-coding DNA sequences of a gene that are transcribed into mRNA, but are spliced out of the rnRNA before the rnRNA is translated into protein. Compare exons. Karyotype: A photomicrograph of an individual’s chromosomes arranged in ...
Lec-GenomeAllignment2010
Lec-GenomeAllignment2010

... Figure 1. The difference between positional homology alignment and glocal alignment. Three example linear genomes are broken into genes labeled A,B,C,D, and R. R is a multicopy (repetitive) gene, with different copies labeled using numeric subscripts. Each copy of R is assumed to be identical in se ...
Karyotyping, FISH and CGH array
Karyotyping, FISH and CGH array

... Cytogenetics is the study of genetic material at the cellular level; molecular genetics studies the structure and function of genes at a molecular level (DNA). The various techniques used vary in their clinical application. This article is a brief summary of the indications for the most commonly-use ...
Lecture 6 S - BEHESHTI MAAL
Lecture 6 S - BEHESHTI MAAL

... 1. Silent mutation: no effect on protein (remember- several codons code for the same amino acid) 2. Missense mutation: codon has changed and different amino acid is incorporated 3. Nonsense mutation: codon has changed to a stop codon ...
genetics_topics_videos_casestudies_table.
genetics_topics_videos_casestudies_table.

Introduction to Molecular Cell Biology (not tought by SK in 2010)
Introduction to Molecular Cell Biology (not tought by SK in 2010)

... Mitochondria have their own DNA, which replicates independent of the nuclear DNA 2. Genetic code of the mitochondria is different from the main code of the cell 3. Mitochondria have their own ribosomes on which some of the mitochondrial proteins are produced. Others are imported from the outside 4. ...
Dot points 5.1-5.5
Dot points 5.1-5.5

... 6. A.I changes the genetic composition of animals e.g a few selected genes become common in the herd, other alleles are lost. Also can result in loss of genetic variation (could be serious if e.g disease resistance is lost). Artificial Pollination - Plants 1. Mendel used artificial pollination (pea ...
Recombinant DNA Answer Key
Recombinant DNA Answer Key

Presentation Slides - Genetics in Primary Care Institute
Presentation Slides - Genetics in Primary Care Institute

... • DNA is converted into RNA and then translated into protein • DNA bases are “read” in groups of three • Each codon (three bases) is specific for a single amino acid ...
Cloning vectors share four common properties
Cloning vectors share four common properties

... (kilobases or kb [1000bp]) ...
Unit 11.1 Gene Transfer
Unit 11.1 Gene Transfer

... What are the basic functions of DNA and RNA? A. DNA - deoxyribonucleic acid is a very complex substance composed of large molecules that are capable of being put together in an almost unlimited number of ways. B. DNA - make up chromosomes. Chromosomes are contributed by each parent and determine how ...
Title
Title

... increase tolerance to stress, increase yield, enhance the value of the end product by enriching it in desired biochemicals such as essential amino acids, and otherwise make plants more useful. Transgenic technology is possible because a gene in DNA will make the protein for which it is designed in a ...
Slide 1
Slide 1

... • This kind of DNA is used to create the DNA Fingerprint because it contains the most variability and STRs ...
What is a Genetic Marker?
What is a Genetic Marker?

... not influenced by selection), and described and ...
10/24 - bio.utexas.edu
10/24 - bio.utexas.edu

... After RT, PCR will amplify the gene or DNA ...
Molecular Genetics DNA Functions Replication Molecular Genetics
Molecular Genetics DNA Functions Replication Molecular Genetics

... Information • Genetic information in DNA molecule resides in sequence of nucleotides. • Gene - Segment of DNA that directs protein ...
Pombe.mating.hm
Pombe.mating.hm

... This imprinted DNA is replicated as a template for the leading strand. DNApol is stalled upon encountering UU and a double-stranded break is introduced in H1 of mat1. This double-stranded break is repaired via gene conversion. Where the 3’ end of the broken strand is resected and then the resulting ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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