• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Yeaman Commentary on Parchman et al 2013
Yeaman Commentary on Parchman et al 2013

... excess of M. vitellinus ancestry. For the introgression parameter b, they found 203 outlier loci with b > 0 and 220 outlier loci with b < 0. Of the FST outlier loci, 43 loci were also a outliers, while 117 loci were also b outliers, with significant positive correlations found between FST and absolu ...
Basic Phylogenetics and Tree Building
Basic Phylogenetics and Tree Building

Annotation Practice Activity [Based on materials from the GEP
Annotation Practice Activity [Based on materials from the GEP

... must add up to three. Sometimes there are multiple possible splice sites so you will need to determine the most likely correct one based on maintaining the phase. Phase 1: one of 3 NT left over Phase 2: two of 3 NT left over Phase 0: no left over NT, i.e., complete codon. Note that at positions 5242 ...
A mutation in the Zn-finger of the GAL4
A mutation in the Zn-finger of the GAL4

... contrast, only a 2-fold reduction by glucose is seen with strain Yl 140 (12) and with JA6-1, a strain isogenic to JA6, but harbouring the LAC9 gene of Y1 140 (23). Hence the difference has to be due to the LAC9 allele and we set out to isolate the LAC9 gene of JA6 (LAC9-2). We used the cloned LAC9-J ...
Ch.-15-Lecture
Ch.-15-Lecture

... • Comparison of the DNA sequences and chromosome organization of related genes from different species helps identify elements essential for their functions • If a human gene has an unknown function • researchers can often deduce its role by studying the equivalent gene in another species, such as a ...
RNA sequencing - Bioinformatics.ca
RNA sequencing - Bioinformatics.ca

... – Genome may be constant but an experimental condition has a pronounced effect on gene expression • e.g. Drug treated vs. untreated cell line • e.g. Wild type versus knock out mice ...
Table 3.1. List of suppliers of restriction enzymes. Name of
Table 3.1. List of suppliers of restriction enzymes. Name of

XRCC3 promotes homology-directed repair of DNA
XRCC3 promotes homology-directed repair of DNA

... Homology-directed repair of DNA damage has recently emerged as a major mechanism for the maintenance of genomic integrity in mammalian cells. The highly conserved strand transferase, Rad51, is expected to be critical for this process. XRCC3 possesses a limited sequence similarity to Rad51 and intera ...
Exercise 1 - EuPathDB Workshop
Exercise 1 - EuPathDB Workshop

... c. Once you find one, click on the gene and go to the gene page. It might be helpful to open the gene page in a new window or tab. d. Scroll down to the bottom of the page to the “Sequences” section. e. Copy the amino acid sequence and go back to EuPathDB (if you have not done so already, it might b ...
Body maps on the human genome | SpringerLink
Body maps on the human genome | SpringerLink

... there is an antero-posterior progression, a “trend of trends”. Figure 4 includes the brain genes distribution of Figure 2, and the ovary genes distribution of Figure 3, along with the other tissue gene head-tail gradients. The relationship between tissue-locations in the body and gene-positions in t ...
chromosome mutations.
chromosome mutations.

... Mutations May Lead to New Alleles Changes to genetic material in somatic cells are not passed on to offspring— the new allele may cause a defect in an individual, but will not affect future generations. However, mutations in germ-line cells (gametic mutations) produce alleles that can be inherited ...
Simulation of Gene Splicing (Genetic Engineering
Simulation of Gene Splicing (Genetic Engineering

... What sticky ends have you made on the human DNA containing the growth hormone gene? What sticky ends have you made on the bacterial DNA (plasmid)? Compare the two. What do you observe? Once the recombinant DNA you just constructed was in existence, the next step would be to insert it into a new bact ...
Instructions fro BLAST Alignment of sequences
Instructions fro BLAST Alignment of sequences

... 11. Find the Alignment View and use the drop-down menu to choose “Query-anchored with dots for identities.” The query is the reference sequence. The query-anchored view shows the reference sequence at the top with the subject sequence aligned below (i.e., the family member’s sequence or a patient’s ...
Review Transposons as tools for functional genomics
Review Transposons as tools for functional genomics

... T-DNA mutagenesis. The insertions generated by transposons are generally single intact elements, which lend themselves easily to molecular analysis. Such insertions are also less likely to result in artefactual patterns of expression if the transposon is being used as a gene trap or enhancer trap. A ...
Transformation of the bacterium E. coli using a gene for green
Transformation of the bacterium E. coli using a gene for green

... 12. While the cells are incubating, your teacher will pass a UV lamp over the pGREEN DNA solution. Note your observations on the student activity sheet and complete questions 1-3. 13. Following incubation, "heat shock" the cells. It is essential that the cells receive a sharp and distinct shock. a. ...
Mossbourne Community Academy A
Mossbourne Community Academy A

... Complete the diagram to show the chromosomes in one cell that could be produced from the cell in Figure 2 as a result of meiosis. ...
Mutations - Warren County Schools
Mutations - Warren County Schools

... • Cystic fibrosis is a severe, genetically determined disease that involves both the lungs and the gastrointestinal tract. It occurs in about one in every two thousand births among white children and at a far lower rate in asian and black children. There are now more than 500 different mutations kn ...
Dangerous Ideas and Forbidden Knowledge, Spring 2005 Lab 2
Dangerous Ideas and Forbidden Knowledge, Spring 2005 Lab 2

... make enough to study. Prior to PCR, this would have been impossible! This dramatic amplification is possible because of the structure of DNA, and the way in which cells naturally copy their own DNA. DNA in our cells exists as a double-stranded molecule. These two strands, or sequences of bases, bind ...
DNA technologies
DNA technologies

... Restriction Endonucleases break the 3',5' phosphodiester bonds between nucleotides. Different enzymes break this bond on different sides of the bond. ...
ppt - University of Illinois at Urbana
ppt - University of Illinois at Urbana

... increasing or decreasing. We will choose to declare them as decreasing with possible exception of the strips with 0 and n+1 ...
ddPCR
ddPCR

... a potential game changer in the fields of life science and clinical diagnostics  It is a method that uses droplet technology to divide complex samples into small, manageable sub-units  This technology is (so far) used for DNA and RNA, but should soon expand to other targets  It provides absolute ...
DNA sequence of the control region of phage D108: the N
DNA sequence of the control region of phage D108: the N

... described by Priess et_ al_. (3) were searched for potential promoter sequences with the computer program of Mulligan et_ al. (10). Potential promoters with a spacing of 16 to 19 nucleotides between the -10 and -35 regions and a promoter score above 50% were listed. The Mu leftward promoter at posit ...
B - Zanichelli
B - Zanichelli

... 46 chromosomes, each one of us is unique. The eukaryotic genome contains many repeated sequences, and between individuals the repeat frequency may differ, offering one way to differentiate individuals. Differences in a single base pair due to DNA replication errors or random mutations also distingui ...
TITLE: Survey of Misannotations and Pseudogenes in the
TITLE: Survey of Misannotations and Pseudogenes in the

... existence of pseudogenes. This makes it difficult to conduct accurate research with this data. In the preliminary research, misannotations in the introns of Arabidopsis thaliana (Arabidopsis Genome Initiative, 2000) have been assessed using the protein kinase domains. The protein kinase family was c ...
Heredity The passing of traits from parent to offspring
Heredity The passing of traits from parent to offspring

... DNA tools can be used to isolate specific genes from the rest of the genome. (Genome = the total DNA present in the nucleus of each cell) 3 Main DNA tools for genetic engineering: 1. Restriction Enzymes 2. EcoRI 3. Gel Electrophoresis ...
< 1 ... 122 123 124 125 126 127 128 129 130 ... 445 >

Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report