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Genetics and Biotechnology
Genetics and Biotechnology

... Why is polymerase chain reaction (PCR) one of the most powerful tools used by scientists? A. It can be used to identify errors in DNA sequences and predict the function of genes. B. It can detect a single DNA molecule in a sample and make millions of copies of it. C. It creates large amounts of reco ...
Chapter 7 Molecular Genetics: From DNA to Proteins
Chapter 7 Molecular Genetics: From DNA to Proteins

... process in which DNA is copied. It occurs during the synthesis (S) phase of the eukaryotic cell cycle. DNA replication begins when an enzyme breaks the bonds between complementary bases in DNA (see Figure 7.5). This exposes the bases inside the molecule so they can be “read” by another enzyme and us ...
«5В070100» –«Биотехнология»
«5В070100» –«Биотехнология»

... development of plant-made pharmaceuticals. Biotechnology is also commonly associated with landmark breakthroughs in new medical therapies to treat hepatitis B, hepatitis C, cancers, arthritis, haemophilia, bone fractures, multiple sclerosis, and cardiovascular disorders. The biotechnology industry h ...
- :: FAPERTA UGM
- :: FAPERTA UGM

... Edible vaccines are vaccines produced in plants that can be administered directly through the ingestion of plant materials containing the vaccine. Eating the plant would then confer immunity against diseases. Edible vaccines produced by transgenic plants are attractive for many reasons. The cost ass ...
Biocyc-GMOD
Biocyc-GMOD

...  Cross-species comparison of individual pathways and reactions ...
Mendelian Inheritance
Mendelian Inheritance

... It is important to describe the features of mitochondrial genetics to understand the possible phenotypic outcomes. First, mtDNA is located in the cytoplasm. Only the cytoplasm from the egg is transmitted to the zygote, sperm rarely contribute mtDNA to the zygote. Thus, mothers and all their offsprin ...
Genetic engineering of human FSH (Gonal
Genetic engineering of human FSH (Gonal

... single alteration (mutation) in the amino acids sequence can render the protein inactive. In order for many of the proteins to be able to carry out their functions correctly, they must have a particular 3D structure. For instance, with an enzyme, certain amino acids in the structure must be position ...
Dragons are a curious type of creature. Amazingly
Dragons are a curious type of creature. Amazingly

... 2. I can explain the difference between homozygous and heterozygous; dominant and recessive; and phenotype and genotype. 3. I can predict the possible outcomes of various genetic combinations when used in monohybrid and dihybrid crosses (Punnett Squares). 4. I can explain what a nondisjunction is an ...
Histological identifications of lesions
Histological identifications of lesions

... PCR conditions were used for all the microsatellite markers. Genomic DNA was amplified by a touchdown PCR with 25 μl reaction mixture. Initial denaturation at 940 C for 5 minutes, 11 cycles of 950 C for 20 sec, 650 C to 560 C for 55 sec and 720 C for 20 seconds, then 30 cycles at 900 C for 20 sec, 5 ...
Chapter 14 Lecture Notes: Nucleic Acids
Chapter 14 Lecture Notes: Nucleic Acids

... 19. Given the primary structure of DNA or mRNA, use the genetic code table to predict the sequence of amino acids in the polypeptide that would be produced in translation. 20. Describe the three types of RNA and understand the role of each in translation. 21. Define the term “gene expression.” 22. D ...
Lab 1 Artificial Selection The purpose of a particular investigation
Lab 1 Artificial Selection The purpose of a particular investigation

... 2. Why were restriction enzymes used in this experiment? The same restriction enzymes were used to cut the plamid and the genes in order to make sure that the plasmid will take up the gene. They will have complementary “sticky” ends. 3. If DNA ligase was not used during the preparation of the recom ...
Finding the genes that direct mammalian development
Finding the genes that direct mammalian development

... be identified by dominant alleles. A larger fraction of the genes that are important for mouse development can be discovered by looking for recessive mutations that disrupt normal embryonic development. A genome-wide screen for recessive phenotypes requires one more generation of crosses than the re ...
Bioinformatics - Sequences and Computers
Bioinformatics - Sequences and Computers

... Learn how information-bearing sequences are different from random sequences and become familiar with bioinformatics tools for the analysis of sequences. Language and DNA use sequences to communicate information. The sequence elements in language are letters and punctuation, in DNA they are the nucle ...
Creating mutant flies
Creating mutant flies

... Small pieces of DNA that can move from one site in the genome to another - ALL organisms have them (about 45% of our genome: transposon remnants!) - Jumping genes, Selfish DNA - Mechanism for evolutionary change ...
The Origin of the Jingwei Gene and the Complex Modular Structure
The Origin of the Jingwei Gene and the Complex Modular Structure

... jgw is a newly evolved functional gene. Furthermore, molecular characterization showed that the insertion of the Adh retrosequence recruited nearby preexisting exons and introns and thereby created a chimerical gene structure in a standard form of exon shuffling. What is the source of the recruited ...
B2.1 Mark Scheme
B2.1 Mark Scheme

... the following points it is less active/activity only 6 arbitrary units (1) (starting to) denature (1) active site is changing shape (1) cannot bind to its substrate as well at this pH (1) ...
Developments in Mutation Assisted Plant Breeding
Developments in Mutation Assisted Plant Breeding

... Another bottleneck to induced crop mutations relates to quality and the inherent recessive nature of mutations. This leads to the masking of the mutation events in the appearance of the mutants by the dominant allele at the same gene locus. In a heterozygous background therefore, phenotypic manifest ...
Diagnostic Clinical Genome and Exome Sequencing
Diagnostic Clinical Genome and Exome Sequencing

... been ordered for patients, with the goal of establishing diagnoses for rare, clinically unrecognizable, or puzzling disorders that are suspected to be genetic in origin. We anticipate increases in the use of CGES, the key attribute of which — its breadth — distinguishes it from other forms of labora ...
Lecture#29 - RFLP-2 - Locating Genes in Large Genomes Using
Lecture#29 - RFLP-2 - Locating Genes in Large Genomes Using

... RFLPs in Pedigree analysis Advantages of RFLP analysis in human applications 1. An RFLP can be found at almost every location in a genome. - not dependent on a gene with a phenotype. - probe has to be unique (not repeated DNA sequences) - try many different restriction-enzyme/probe combinations - an ...
PART II
PART II

... 4.7. Safety assessment for GM crops and foods Food safety is a shared responsibility of industry, farmers, and regulatory authorities. As there is normally no history of safe use for a novel food or food derived from a GM crop, but may be available for both the conventional food and the introduced p ...
Slide 1
Slide 1

... Genes specify the identity and order of amino acids in a polypeptide chain The sequence of amino acids in a protein determines its three-dimensional shape and function Some proteins contain more than one polypeptide coded for by different genes ...
S Diagnostic Clinical Genome and Exome Sequencing review article
S Diagnostic Clinical Genome and Exome Sequencing review article

... been ordered for patients, with the goal of establishing diagnoses for rare, clinically unrecognizable, or puzzling disorders that are suspected to be genetic in origin. We anticipate increases in the use of CGES, the key attribute of which — its breadth — distinguishes it from other forms of labora ...
Slide 1
Slide 1

...  Basis for clinical prognosis, potential medical complications.  Guidance regarding treatment and long-term medical management, particularly in the young infant.  Definitive information to guide genetic counseling of families. ...
Slide 1
Slide 1

... Square and decide what percent of the offspring will be black and brown. ...
Chromosomes, Genes and DNA
Chromosomes, Genes and DNA

... How do genes make proteins? Genes are made of DNA. Proteins are made of amino acids. Each amino acid is coded for by its own special sequence of three bases called a triplet: ...
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Genome editing

Genome editing, or genome editing with engineered nucleases (GEEN) is a type of genetic engineering in which DNA is inserted, replaced, or removed from a genome using artificially engineered nucleases, or ""molecular scissors."" The nucleases create specific double-stranded break (DSBs) at desired locations in the genome, and harness the cell’s endogenous mechanisms to repair the induced break by natural processes of homologous recombination (HR) and nonhomologous end-joining (NHEJ). There are currently four families of engineered nucleases being used: Zinc finger nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), the CRISPR/Cas system, and engineered meganuclease re-engineered homing endonucleases.It is commonly practiced in genetic analysis that in order to understand the function of a gene or a protein function one interferes with it in a sequence-specific way and monitors its effects on the organism. However, in some organisms it is difficult or impossible to perform site-specific mutagenesis, and therefore more indirect methods have to be used, such as silencing the gene of interest by short RNA interference (siRNA) . Yet gene disruption by siRNA can be variable and incomplete. Genome editing with nucleases such as ZFN is different from siRNA in that the engineered nuclease is able to modify DNA-binding specificity and therefore can in principle cut any targeted position in the genome, and introduce modification of the endogenous sequences for genes that are impossible to specifically target by conventional RNAi. Furthermore, the specificity of ZFNs and TALENs are enhanced as two ZFNs are required in the recognition of their portion of the target and subsequently direct to the neighboring sequences.It was chosen by Nature Methods as the 2011 Method of the Year.
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