Studying copy number variations using a nanofluidic platform
... the PCR experiment. One of the important applications of the digital array is absolute quantitation (14,15). The DNA molecules in each mixture are randomly partitioned into the 765 chambers of each panel. The chip is then thermocycled on Fluidigm’s BioMark system and the positive chambers that origi ...
... the PCR experiment. One of the important applications of the digital array is absolute quantitation (14,15). The DNA molecules in each mixture are randomly partitioned into the 765 chambers of each panel. The chip is then thermocycled on Fluidigm’s BioMark system and the positive chambers that origi ...
References - UTH e
... 1. PCR enables rapid amplification of template DNA for screening of uncharacterized mutations Because of its rapidity and simplicity, PCR is ideally suited to providing numerous DNA templates for mutation screening. Partial DNA sequences, at the genomic or the cDNA level, from a gene associated with ...
... 1. PCR enables rapid amplification of template DNA for screening of uncharacterized mutations Because of its rapidity and simplicity, PCR is ideally suited to providing numerous DNA templates for mutation screening. Partial DNA sequences, at the genomic or the cDNA level, from a gene associated with ...
Expanding Yeast Knowledge Online.
... responsible for maintaining the official S. cerevisiae Gene Registry. The Gene Registry helps to maintain yeast gene names in a standardized format, and SGD mediates resolution of gene naming conflicts. On-line submission forms to register gene names are found at the SGD site. In addition, yeast res ...
... responsible for maintaining the official S. cerevisiae Gene Registry. The Gene Registry helps to maintain yeast gene names in a standardized format, and SGD mediates resolution of gene naming conflicts. On-line submission forms to register gene names are found at the SGD site. In addition, yeast res ...
Controlling complexity: the clinical relevance of mouse complex
... reflects perfectly a human disease state. One should not always expect that a mutation in an orthologous gene in the mouse results in the same phenotype observed in humans. But even in these cases, one will obtain important insights into its biological functions. On the other hand, the expectations ...
... reflects perfectly a human disease state. One should not always expect that a mutation in an orthologous gene in the mouse results in the same phenotype observed in humans. But even in these cases, one will obtain important insights into its biological functions. On the other hand, the expectations ...
The Schistosoma gene discovery program: state of the art
... S. japonicum ESTs are present in the database for ESTs (dbEST release 123199 of December 31, 1999) [http://www.ncbi.nlm.nih.gov/dbEST/index.html]. Cluster analyses of 8193 S. mansoni sequences and 891 S. japonicum sequences (these include ESTs and gene sequences) were performed by D Johnston (the SG ...
... S. japonicum ESTs are present in the database for ESTs (dbEST release 123199 of December 31, 1999) [http://www.ncbi.nlm.nih.gov/dbEST/index.html]. Cluster analyses of 8193 S. mansoni sequences and 891 S. japonicum sequences (these include ESTs and gene sequences) were performed by D Johnston (the SG ...
Genetic Characterization of Insulin Growth Factor
... but most quantitative traits are usually controlled by multiple genes (polygenic) and influenced by environmental factors [2]. In quantitative genetics, there are a number of single genes associated with mammary or muscle growth, development and function which were studied as excellent candidates fo ...
... but most quantitative traits are usually controlled by multiple genes (polygenic) and influenced by environmental factors [2]. In quantitative genetics, there are a number of single genes associated with mammary or muscle growth, development and function which were studied as excellent candidates fo ...
QUESTIONS AND ANSWER TO PROBLEM SETS
... Answer: There are many ethical issues associated with human cloning. Is it the wrong thing to do? Does it conflict an individual’s religious views? And so on. FIGURE 1.3 Concept check: Why is it useful to sort male and female mosquitos? Answer: By sorting the mosquitos, sterile males can be released ...
... Answer: There are many ethical issues associated with human cloning. Is it the wrong thing to do? Does it conflict an individual’s religious views? And so on. FIGURE 1.3 Concept check: Why is it useful to sort male and female mosquitos? Answer: By sorting the mosquitos, sterile males can be released ...
Extraordinary Sequence Divergence at Tsga8, an X
... screen of X-linked genes potentially involved in reproductive isolation in mice to identify putative targets of recurrent positive selection. We then sequenced five very rapidly evolving genes within and between several closely related species of mice in the genus Mus. All five genes were involved i ...
... screen of X-linked genes potentially involved in reproductive isolation in mice to identify putative targets of recurrent positive selection. We then sequenced five very rapidly evolving genes within and between several closely related species of mice in the genus Mus. All five genes were involved i ...
Ribosome reinitiation at leader peptides increases translation of
... analyzed specifically for operons. We are grateful to the reviewer for setting out the former distance problem in the context of the distance between neighboring genes in general in bacterial operons. We have bioinformatics data relevant to the latter distance problem. Several examples of this kind ...
... analyzed specifically for operons. We are grateful to the reviewer for setting out the former distance problem in the context of the distance between neighboring genes in general in bacterial operons. We have bioinformatics data relevant to the latter distance problem. Several examples of this kind ...
Document
... series of reversals to transform one into another • Input: Permutations p and s • Output: A series of reversals r1,…rt transforming p into s, such that t is minimum • t - reversal distance between p and s • d(p, s) = smallest possible value of t, given p, s ...
... series of reversals to transform one into another • Input: Permutations p and s • Output: A series of reversals r1,…rt transforming p into s, such that t is minimum • t - reversal distance between p and s • d(p, s) = smallest possible value of t, given p, s ...
Deep Insight Section Genomic Imprinting: Parental differentiation of the genome
... imprinted genes, in a parent-of-origin-dependent manner, differs from the post-zygotic monoallelic expression of certain genes involved in olfaction and immunity. At present, some 4 score genes are known to be imprinted, and it is estimated that mammalian genomes may contain several hundred imprinte ...
... imprinted genes, in a parent-of-origin-dependent manner, differs from the post-zygotic monoallelic expression of certain genes involved in olfaction and immunity. At present, some 4 score genes are known to be imprinted, and it is estimated that mammalian genomes may contain several hundred imprinte ...
Chapter 12
... Chapter 12 - DNA Technology and the Human Genome How can we use bacteria to manipulate DNA and protein? Recall how a bacterium defends itself when a bacteriophage injects its DNA into a bacterium… The bacterium has enzymes called restriction enzymes that attempt to cut up the bacteriophage DNA befo ...
... Chapter 12 - DNA Technology and the Human Genome How can we use bacteria to manipulate DNA and protein? Recall how a bacterium defends itself when a bacteriophage injects its DNA into a bacterium… The bacterium has enzymes called restriction enzymes that attempt to cut up the bacteriophage DNA befo ...
"Using Model Organism Databases (MODs)". In: Current - SGD-Wiki
... the annotation of gene products in a wide variety of organisms (Blake and Harris, 2008). These controlled vocabularies, known as ontologies, consist of standardized terms (i.e., kinase activity, transsulfuration, mitochondrion, etc.) with controlled definitions, and include all known relationships b ...
... the annotation of gene products in a wide variety of organisms (Blake and Harris, 2008). These controlled vocabularies, known as ontologies, consist of standardized terms (i.e., kinase activity, transsulfuration, mitochondrion, etc.) with controlled definitions, and include all known relationships b ...
epigenetics - Gene Silencing
... any gene in a cell. The immense impact of the discovery of RNAi on biomedical research and its novel medical applications in the future, will be reviewed in this article. Prof. Bertil Daneholt, Professor of Molecular genetics, Karolinska Institute Stockholm, Chairman of the Nobel Assembly has gi ...
... any gene in a cell. The immense impact of the discovery of RNAi on biomedical research and its novel medical applications in the future, will be reviewed in this article. Prof. Bertil Daneholt, Professor of Molecular genetics, Karolinska Institute Stockholm, Chairman of the Nobel Assembly has gi ...
Arabidopsis thaliana: A Model Plant for Genome Analysis
... often lagged behind mutant identification, a number of mutants currently being studied in different laboratories are likely to be defective in the same gene. Progress has nevertheless been made toward establishing community standards for gene nomenclature and mutant analysis to minimize duplication ...
... often lagged behind mutant identification, a number of mutants currently being studied in different laboratories are likely to be defective in the same gene. Progress has nevertheless been made toward establishing community standards for gene nomenclature and mutant analysis to minimize duplication ...
Two Cyp19 (P450 Aromatase) Genes on Duplicated Zebrafish
... branched as expected from the known evolutionary relationships of the species. All fish Cyp19 genes clustered together on the same branch, suggesting that they were all orthologs of the single mammalian Cyp19 gene. The fish branch, however, bifurcated into two subbranches with a high bootstrap value ...
... branched as expected from the known evolutionary relationships of the species. All fish Cyp19 genes clustered together on the same branch, suggesting that they were all orthologs of the single mammalian Cyp19 gene. The fish branch, however, bifurcated into two subbranches with a high bootstrap value ...
Common Long Human Inversion Polymorphism on Chromosome 8p
... the inverted region was estimated to be at least 2.5 Mb and possibly as long as 5.3 Mb. The sequence assembly in this region of 8p is still crude with many gaps, both large and small, and other uncertainties. Sites of the inversion breakpoints are not yet precisely known. From both the CEPH and FISH ...
... the inverted region was estimated to be at least 2.5 Mb and possibly as long as 5.3 Mb. The sequence assembly in this region of 8p is still crude with many gaps, both large and small, and other uncertainties. Sites of the inversion breakpoints are not yet precisely known. From both the CEPH and FISH ...
GENETIC MUTATIONS - Manning's Science
... the DNA is transmitted through cell division. Changes in number or structure of chromosomes Spontaneous Induced Mutagens = UV, X Rays, other radiation; chemicals; heavy metals ...
... the DNA is transmitted through cell division. Changes in number or structure of chromosomes Spontaneous Induced Mutagens = UV, X Rays, other radiation; chemicals; heavy metals ...
PDF
... where TP, TN, FP and FN denote number of true positives, true negatives, false positives and false negatives, respecexons with 17% false positives. tively. We find that the accuracy of FirstEF is significantly higher for CpG-related than non-CpG-related genes. To study the performance of FirstEF on ...
... where TP, TN, FP and FN denote number of true positives, true negatives, false positives and false negatives, respecexons with 17% false positives. tively. We find that the accuracy of FirstEF is significantly higher for CpG-related than non-CpG-related genes. To study the performance of FirstEF on ...
Abundance of an mRNA is the average number of molecules per cell
... Complex locus (of D. melanogaster) has genetic properties inconsistent with the function of a gene representing a single protein. Complex loci are usually very large (>100kb) at the molecular level. Complexity is the total length of different sequences of DNA present in a given preparation. Composti ...
... Complex locus (of D. melanogaster) has genetic properties inconsistent with the function of a gene representing a single protein. Complex loci are usually very large (>100kb) at the molecular level. Complexity is the total length of different sequences of DNA present in a given preparation. Composti ...
Construction of an arabidopsis BAC library and isolation of clones
... physical maps of the arabidopsis genome and to the isolation of many genes (Arondel et al., 1992; Putterill et al., 1993). YAC libraries, however, have some problems including chimeric and unstable clones (Neil et al., 1990; Green et al., 1991; Libert et al., 1993; Umehara et al., 1994, Schmidt et a ...
... physical maps of the arabidopsis genome and to the isolation of many genes (Arondel et al., 1992; Putterill et al., 1993). YAC libraries, however, have some problems including chimeric and unstable clones (Neil et al., 1990; Green et al., 1991; Libert et al., 1993; Umehara et al., 1994, Schmidt et a ...
Behold the fowls of the air
... biology, readers will be struck simultaneously by how much is known about avian biochemistry and molecular biology and how much is still to be learned. For example, much can be explained about metabolic adaptations associated with the energy demands of flight, but why are bird lifespans surprisingly ...
... biology, readers will be struck simultaneously by how much is known about avian biochemistry and molecular biology and how much is still to be learned. For example, much can be explained about metabolic adaptations associated with the energy demands of flight, but why are bird lifespans surprisingly ...
Chapter 5
... genome contributed by the germ cells, egg and sperm, contains a large repertoire of immunoglobulin genes 2. Somatic-variation theory: maintained that the genome contains a small number of immunoglobulin genes, from which a large number of Ab specificities are generated in the somatic cells by mutati ...
... genome contributed by the germ cells, egg and sperm, contains a large repertoire of immunoglobulin genes 2. Somatic-variation theory: maintained that the genome contains a small number of immunoglobulin genes, from which a large number of Ab specificities are generated in the somatic cells by mutati ...
Nontraditional Inheritance
... and myotonic dystrophy) whereas others are more likely to expand when inherited from the father (e.g., Huntington disease). The FMR1 gene also has mitotic instability, so that there may be variation in the size of expansion in different cells and different tissues in the same individual. This is not ...
... and myotonic dystrophy) whereas others are more likely to expand when inherited from the father (e.g., Huntington disease). The FMR1 gene also has mitotic instability, so that there may be variation in the size of expansion in different cells and different tissues in the same individual. This is not ...
Human genome
The human genome is the complete set of nucleic acid sequence for humans (Homo sapiens), encoded as DNA within the 23 chromosome pairs in cell nuclei and in a small DNA molecule found within individual mitochondria. Human genomes include both protein-coding DNA genes and noncoding DNA. Haploid human genomes, which are contained in germ cells (the egg and sperm gamete cells created in the meiosis phase of sexual reproduction before fertilization creates a zygote) consist of three billion DNA base pairs, while diploid genomes (found in somatic cells) have twice the DNA content. While there are significant differences among the genomes of human individuals (on the order of 0.1%), these are considerably smaller than the differences between humans and their closest living relatives, the chimpanzees (approximately 4%) and bonobos. Humans share 50% of their DNA with bananas.The Human Genome Project produced the first complete sequences of individual human genomes, with the first draft sequence and initial analysis being published on February 12, 2001. The human genome was the first of all vertebrates to be completely sequenced. As of 2012, thousands of human genomes have been completely sequenced, and many more have been mapped at lower levels of resolution. The resulting data are used worldwide in biomedical science, anthropology, forensics and other branches of science. There is a widely held expectation that genomic studies will lead to advances in the diagnosis and treatment of diseases, and to new insights in many fields of biology, including human evolution.Although the sequence of the human genome has been (almost) completely determined by DNA sequencing, it is not yet fully understood. Most (though probably not all) genes have been identified by a combination of high throughput experimental and bioinformatics approaches, yet much work still needs to be done to further elucidate the biological functions of their protein and RNA products. Recent results suggest that most of the vast quantities of noncoding DNA within the genome have associated biochemical activities, including regulation of gene expression, organization of chromosome architecture, and signals controlling epigenetic inheritance.There are an estimated 20,000-25,000 human protein-coding genes. The estimate of the number of human genes has been repeatedly revised down from initial predictions of 100,000 or more as genome sequence quality and gene finding methods have improved, and could continue to drop further. Protein-coding sequences account for only a very small fraction of the genome (approximately 1.5%), and the rest is associated with non-coding RNA molecules, regulatory DNA sequences, LINEs, SINEs, introns, and sequences for which as yet no function has been elucidated.