The Endoplasmic Reticulum Glucosyltransferase
... CI2 conformation (13). We had previously determined that a significant GT activity was only attained with the fragment of 53 residues in length, the first ones to display long range native interactions (8). We now focused on GCI2 fragments having from 53 to 64 (full-length) amino acid residues. Thei ...
... CI2 conformation (13). We had previously determined that a significant GT activity was only attained with the fragment of 53 residues in length, the first ones to display long range native interactions (8). We now focused on GCI2 fragments having from 53 to 64 (full-length) amino acid residues. Thei ...
Classifier ensembles for protein structural class prediction with
... include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncontrolled (often high) sequence homology. This tends to result in an ...
... include auto-correlation functions based on non-bonded residue energy [7], polypeptide composition [26,37,44], and functional domain composition [16]. However, these algorithms are often only tested on very small datasets, with uncontrolled (often high) sequence homology. This tends to result in an ...
Protein reutilisation in corms of Colchicum autumnale
... dissolved in buffer used and dialysed. All operations were carried out at 4 ◦C. Determination of proteolytic activities To determine aminopeptidase activity the L-alanine pnitroanilide and L-leucine p-nitroanilide (Sigma, Richmond, USA) were used as substrates. Standard reaction mixture at final volum ...
... dissolved in buffer used and dialysed. All operations were carried out at 4 ◦C. Determination of proteolytic activities To determine aminopeptidase activity the L-alanine pnitroanilide and L-leucine p-nitroanilide (Sigma, Richmond, USA) were used as substrates. Standard reaction mixture at final volum ...
(CH14) Translation (Slides)
... enhancement of peptide bond formation is due to substrate positioning by the 23S rRNA within the active site, rather than chemical ...
... enhancement of peptide bond formation is due to substrate positioning by the 23S rRNA within the active site, rather than chemical ...
A minimal gene set for cellular life derived by comparison of
... billion years of evolution (6). H. influenzae is also a parasitic bacterium with a relatively small genome that is 1.83 megabases long and contains about 1700 protein-coding genes; its evolution apparently included a number of gene elimination events (1, 3). Therefore, the genes that are conserved i ...
... billion years of evolution (6). H. influenzae is also a parasitic bacterium with a relatively small genome that is 1.83 megabases long and contains about 1700 protein-coding genes; its evolution apparently included a number of gene elimination events (1, 3). Therefore, the genes that are conserved i ...
Tubulin Subunit Carboxyl Termini Determine Polymerization Efficiency
... acidic amino acids, especially glutamic acid (1,2 ) . At physiological pH, this sequence will be highly charged and probably in an extended conformation (8). If so, it should be more accessible than the folded region of the protein to attack by enzymes, including but not limited to those involved in ...
... acidic amino acids, especially glutamic acid (1,2 ) . At physiological pH, this sequence will be highly charged and probably in an extended conformation (8). If so, it should be more accessible than the folded region of the protein to attack by enzymes, including but not limited to those involved in ...
Midterm 1 - U of L Class Index
... poly(Glu) can form a helix with the side chains sticking out into solution and interacting with the surrounding water. Similarly, at pH < 9, the amino groups of poly(Lys) are fully protonated; repulsion among these positively charged groups also leads to unfolding of the α helix. Above pH 10.5 (pKa ...
... poly(Glu) can form a helix with the side chains sticking out into solution and interacting with the surrounding water. Similarly, at pH < 9, the amino groups of poly(Lys) are fully protonated; repulsion among these positively charged groups also leads to unfolding of the α helix. Above pH 10.5 (pKa ...
Side-chain hydrophobicity scale derived from transmembrane
... Two exceptions are aspartic acid and glutamic acid, whose energetics are underrepresented in our whole-protein scale because the pH of our experiments was 3.8, which is close to the pKa values for model compounds of Asp and Glu side chains (29). It is reasonable to expect that a significant populati ...
... Two exceptions are aspartic acid and glutamic acid, whose energetics are underrepresented in our whole-protein scale because the pH of our experiments was 3.8, which is close to the pKa values for model compounds of Asp and Glu side chains (29). It is reasonable to expect that a significant populati ...
Welcome
... The detection of DDI is an important research area in patient safety since these interactions can become very dangerous and increase health care costs. Although there are different databases supporting health care professionals in the detection of DDI, these databases are rarely complete, since thei ...
... The detection of DDI is an important research area in patient safety since these interactions can become very dangerous and increase health care costs. Although there are different databases supporting health care professionals in the detection of DDI, these databases are rarely complete, since thei ...
Differential protein profile in sexed bovine semen: shotgun
... sperm cells.14,15 More recently, an extensive proteomic investigation of sexed sperm cells by 2-DE/MS shed light on differentially expressed proteins between bull X and Y spermatozoa including proteins involved in energy metabolism, cellular defense and stress, cytoskeleton and inhibitors of serine ...
... sperm cells.14,15 More recently, an extensive proteomic investigation of sexed sperm cells by 2-DE/MS shed light on differentially expressed proteins between bull X and Y spermatozoa including proteins involved in energy metabolism, cellular defense and stress, cytoskeleton and inhibitors of serine ...
Function and specificity of 14-3-3 proteins in the regulation of
... osmotic stress activation of the enzyme (McMichael et al., 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spinach SPS which inhibits SPS activity (Toroser et al., 1998). The interaction of 14-3-3s and target proteins can ...
... osmotic stress activation of the enzyme (McMichael et al., 1993; Toroser and Huber, 1997). However, there is a sitespeci®c regulatory interaction between 14-3-3 proteins and Ser-229 of spinach SPS which inhibits SPS activity (Toroser et al., 1998). The interaction of 14-3-3s and target proteins can ...
PPT - Hirst Group - The University of Nottingham
... Twin neural networks give a consensus prediction. ...
... Twin neural networks give a consensus prediction. ...
search1
... 2. Increase the Word Size to 20 - 25. With a default Word Size of 7, limiting the number small initial fragments to be extended to HSPs. ...
... 2. Increase the Word Size to 20 - 25. With a default Word Size of 7, limiting the number small initial fragments to be extended to HSPs. ...
PPT File
... Bradykinin (9 a.a.): inhibits inflammation of tissues Thyrotropin-releasing factor (3 a.a.): formed in hypothalamus stimulates the release of thyrotropin from the anterior pituitary ...
... Bradykinin (9 a.a.): inhibits inflammation of tissues Thyrotropin-releasing factor (3 a.a.): formed in hypothalamus stimulates the release of thyrotropin from the anterior pituitary ...
Know Your Feed Terms - Alberta Agriculture and Forestry
... ADIN occurs naturally in all plant material, it is usually considered to be an indicator of heat damage that can occur during storage or processing. Excessive heating of forages and grains causes some of the nitrogen to become irreversibly bound in the fibre. Nitrogen in excessively heated samples i ...
... ADIN occurs naturally in all plant material, it is usually considered to be an indicator of heat damage that can occur during storage or processing. Excessive heating of forages and grains causes some of the nitrogen to become irreversibly bound in the fibre. Nitrogen in excessively heated samples i ...
What is sequence alignment - department of computer & electrical
... Given two strings, and a scoring scheme for evaluating matching letters, find the optimal pairing of letters from one sequence to letters of the other sequence ...
... Given two strings, and a scoring scheme for evaluating matching letters, find the optimal pairing of letters from one sequence to letters of the other sequence ...
Predicted function of the vaccinia virus G5R protein
... therefore are likely to be functional in G5R. The two positively charged residues that are keys for FEN-1 activity are lysine 93 and arginine 100 (Shen et al., 2005). These two residues are absolutely conserved in all poxvirus G5R protein sequences (Supplementary material, A1) and their positions ar ...
... therefore are likely to be functional in G5R. The two positively charged residues that are keys for FEN-1 activity are lysine 93 and arginine 100 (Shen et al., 2005). These two residues are absolutely conserved in all poxvirus G5R protein sequences (Supplementary material, A1) and their positions ar ...
Supporting information. Rat urinary glycoprotein identified from
... change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates. Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase Isoform 1 is detectable at ...
... change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates. Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase Isoform 1 is detectable at ...
Chicken eggshell matrix proteins related to anti
... has been reported that they could be involved in the innate immune response. Members of the LBP/BPI family are proteins which bind to the lipid A portion of lipolysaccharide (LPS) cell wall in gram negative bacteria, leading to death of the bacterium. This interaction could be promoted in OCX-36, si ...
... has been reported that they could be involved in the innate immune response. Members of the LBP/BPI family are proteins which bind to the lipid A portion of lipolysaccharide (LPS) cell wall in gram negative bacteria, leading to death of the bacterium. This interaction could be promoted in OCX-36, si ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.