emboj2009380-sup
... cloning map is shown in Supplementary Figure S9C. The recombinant expression constructs were verified by DNA sequencing, and transformed into E. coli BL-21 (DE3) for the protein expression. The overnight cultures of the respective clones were separately resuspended in M9Y broth (Uechi et al, 2005) s ...
... cloning map is shown in Supplementary Figure S9C. The recombinant expression constructs were verified by DNA sequencing, and transformed into E. coli BL-21 (DE3) for the protein expression. The overnight cultures of the respective clones were separately resuspended in M9Y broth (Uechi et al, 2005) s ...
What are proteins - Assiut University
... A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA. Southwestern blotting, based along the lines of Southern blotting ...
... A Southern blot is a method routinely used in molecular biology for detection of a specific DNA sequence in DNA samples. The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA. Southwestern blotting, based along the lines of Southern blotting ...
Lecture 2 Slides
... carboxyl group of another to form a peptide bond (=amide linkage) with the elimination of a water molecule. • The polarity of the molecule is retained. The monomers are all joined together in the same orientation. The polypeptide chain has polarity. • The polypeptide contains a free amino group at o ...
... carboxyl group of another to form a peptide bond (=amide linkage) with the elimination of a water molecule. • The polarity of the molecule is retained. The monomers are all joined together in the same orientation. The polypeptide chain has polarity. • The polypeptide contains a free amino group at o ...
week 10_protein
... protonation of some protein side group, which alter/disrupt hydrogen bonding & salt bridge. As a protein approaches its isoelectric point, it becomes insoluble and precipitates from solution. Organic solvents – water-soluble organic solvents eg. Ethanol interfere with hydrophobic interaction because ...
... protonation of some protein side group, which alter/disrupt hydrogen bonding & salt bridge. As a protein approaches its isoelectric point, it becomes insoluble and precipitates from solution. Organic solvents – water-soluble organic solvents eg. Ethanol interfere with hydrophobic interaction because ...
CHEM501- Introduction to Biochemistry – Exam 1 w
... All subunits must be identical. Many have regulatory roles. Some oligomeric proteins can further associate into large fibers. Some subunits may have nonprotein prosthetic groups. ...
... All subunits must be identical. Many have regulatory roles. Some oligomeric proteins can further associate into large fibers. Some subunits may have nonprotein prosthetic groups. ...
Datasheet - Santa Cruz Biotechnology
... MND1 localizes to chromatin during meiotic prophase and preferentially binds double-stranded DNA. MND1 forms a stable heterodimeric complex with HOP2, which binds DNA to activate the recombinase activity of DMC1 and RAD51. Disruption of the MND1-HOP2 complex leads to failure in meiotic recombination ...
... MND1 localizes to chromatin during meiotic prophase and preferentially binds double-stranded DNA. MND1 forms a stable heterodimeric complex with HOP2, which binds DNA to activate the recombinase activity of DMC1 and RAD51. Disruption of the MND1-HOP2 complex leads to failure in meiotic recombination ...
Feeds and Feed Label
... Sources of essential fatty acids Serve as a carrier for absorption of fat- soluble vitamins ...
... Sources of essential fatty acids Serve as a carrier for absorption of fat- soluble vitamins ...
Unfolded Protein Response (UPR)
... Isolation of two clones which encode XBP1 (X-box binding protein 1) and ATF6a (activating transcription factor a) containing a bZIP motif as a DNA-binding domain, separately. ...
... Isolation of two clones which encode XBP1 (X-box binding protein 1) and ATF6a (activating transcription factor a) containing a bZIP motif as a DNA-binding domain, separately. ...
AP review
... True positives (TP) – nucleotides, which are correctly predicted to be within the gene. Actual positives (AP) – nucleotides, which are located within the actual gene. Predicted positives (PP) – nucleotides, which are predicted in the gene. Sensitivity = TP / AP Specificity = TP / PP ...
... True positives (TP) – nucleotides, which are correctly predicted to be within the gene. Actual positives (AP) – nucleotides, which are located within the actual gene. Predicted positives (PP) – nucleotides, which are predicted in the gene. Sensitivity = TP / AP Specificity = TP / PP ...
Powerpoint slides
... 1. There is almost no correlation between the number of genes and organism’s complexity. ...
... 1. There is almost no correlation between the number of genes and organism’s complexity. ...
Uniform Isotope Labeling of Eukaryotic Proteins in Methylotrophic
... The double (13C,15N) uniform isotope labeling of soluble secreted proteins in Pichia relies on a very straightforward protocol, in which the carbon source at the pre-induction growth phase (e.g. 13C6-glucose) is replaced by 13C-methanol in the post-induction phase.16 Concentrations and timing of add ...
... The double (13C,15N) uniform isotope labeling of soluble secreted proteins in Pichia relies on a very straightforward protocol, in which the carbon source at the pre-induction growth phase (e.g. 13C6-glucose) is replaced by 13C-methanol in the post-induction phase.16 Concentrations and timing of add ...
Integral proteins
... Integral proteins have domains that extend into the hydrocarbon core of the membrane. Often they span the bilayer. Intramembrane domains have largely hydrophobic surfaces, that interact with membrane lipids Amphipathic detergents are required for solubilization of integral proteins from membranes ...
... Integral proteins have domains that extend into the hydrocarbon core of the membrane. Often they span the bilayer. Intramembrane domains have largely hydrophobic surfaces, that interact with membrane lipids Amphipathic detergents are required for solubilization of integral proteins from membranes ...
Document
... For each alignment, the probability that that each amino acid residue would occur in such an environment is calculated based on observed preferences in determined structures. § Rationale: • Limited number of basic folds found in nature • Amino acid preferences for different structural environments p ...
... For each alignment, the probability that that each amino acid residue would occur in such an environment is calculated based on observed preferences in determined structures. § Rationale: • Limited number of basic folds found in nature • Amino acid preferences for different structural environments p ...
Chemistry part 2
... Proteins are made of amino acids • All amino acids have a backbone and a side group (“R” group) • Backbone: amino group, carboxyl group, central carbon • Side group: there are 20 different kinds, each with different chemical properties ...
... Proteins are made of amino acids • All amino acids have a backbone and a side group (“R” group) • Backbone: amino group, carboxyl group, central carbon • Side group: there are 20 different kinds, each with different chemical properties ...
Macromolecule Review - Mr. Dudley`s Website
... A dog gets many nutrients from its food including amino acids. Which of these can be built directly using the amino acids? ...
... A dog gets many nutrients from its food including amino acids. Which of these can be built directly using the amino acids? ...
Overview of Metaboli.. - Frozen Crocus Productions
... Glycolysis: produces pyruvate for acetyl CoA production in mitochondria, produces NADH (electrons) for ETC in mitochondria, anaerobic production of ATP MK & CPK: anaerobic production of ATP TCA: accepts acetyl-CoA for citrate synthesis, production of NADH (electrons) & TCAintermediates can be used f ...
... Glycolysis: produces pyruvate for acetyl CoA production in mitochondria, produces NADH (electrons) for ETC in mitochondria, anaerobic production of ATP MK & CPK: anaerobic production of ATP TCA: accepts acetyl-CoA for citrate synthesis, production of NADH (electrons) & TCAintermediates can be used f ...
Gene Duplication in the Mo-Fe Protein of Nitrogenase
... plants • catalyzes the conversion of molecular nitrogen (N2) from the air into ammonia (NH3). • It is found in a variety of bacteria, some of them symbiotic with plants. ...
... plants • catalyzes the conversion of molecular nitrogen (N2) from the air into ammonia (NH3). • It is found in a variety of bacteria, some of them symbiotic with plants. ...
Basic Biochemistry - Personal Webspace for QMUL
... ____ by applying an electric charge through a polymer gel A polyacrylamide gel is almost always used The technique is known as: Polyacrylamide Gel Electrophoresis ==> PAGE Polyacrylamide is chemically inert Figure 3-7b, page 71 (3-7b, page 74) The gel forms as ‘SPAGHETTI-LIKE’ STRANDS The ...
... ____ by applying an electric charge through a polymer gel A polyacrylamide gel is almost always used The technique is known as: Polyacrylamide Gel Electrophoresis ==> PAGE Polyacrylamide is chemically inert Figure 3-7b, page 71 (3-7b, page 74) The gel forms as ‘SPAGHETTI-LIKE’ STRANDS The ...
Interactome
In molecular biology, an interactome is the whole set of molecular interactions in a particular cell. The term specifically refers to physical interactions among molecules (such as those among proteins, also known as protein-protein interactions) but can also describe sets of indirect interactions among genes (genetic interactions). Mathematically, interactomes are generally displayed as graphs.The word ""interactome"" was originally coined in 1999 by a group of French scientists headed by Bernard Jacq. Though interactomes may be described as biological networks, they should not be confused with other networks such as neural networks or food webs.