SUC1 and SUC2: two sucrose transporters from Arabidopsis
... phloem cells via a sucrose-H+ symporter. This paper describes functional expression in Saccharomyces cerevisiae of two cDNAs encoding energy-dependent sucrose transporters from the plasma membrane of Arabidopsis thaliane, SUCl and SUC2. Yeast cells transformed with vectors allowing expression of eit ...
... phloem cells via a sucrose-H+ symporter. This paper describes functional expression in Saccharomyces cerevisiae of two cDNAs encoding energy-dependent sucrose transporters from the plasma membrane of Arabidopsis thaliane, SUCl and SUC2. Yeast cells transformed with vectors allowing expression of eit ...
... To determine the identity of the eDNA inserts, they were subcloned into pGEM-4Z plasmid vector and sequenced from both orientations using the dideoxy chain termination method [19]. A search of the gene data bank revealed extensive sequence homology between one of the inserts and the L-chain inhibito ...
Biology Review
... There are two types of nucleic acids: DNA (deoxyribonucleic acid) stores genetic information in the cell and organism-it replicates and gets transmitted to other cells when they divide and also when an organism reproduces. DNA provides directions for its own replication. DNA also directs synthes ...
... There are two types of nucleic acids: DNA (deoxyribonucleic acid) stores genetic information in the cell and organism-it replicates and gets transmitted to other cells when they divide and also when an organism reproduces. DNA provides directions for its own replication. DNA also directs synthes ...
Statistical Analysis of Amino Acid Patterns in Transmembrane
... lost upon packing, as postulated by MacKenzie & Engelman (1998), could also limit the number of conformations suitable for interaction. The existence of correlations between residues has been suggested in previous statistical studies on predicted TM sequence databases (Arkin & Brunger, 1998; Landolt ...
... lost upon packing, as postulated by MacKenzie & Engelman (1998), could also limit the number of conformations suitable for interaction. The existence of correlations between residues has been suggested in previous statistical studies on predicted TM sequence databases (Arkin & Brunger, 1998; Landolt ...
Molecular Recognition of Amino Acids, Peptides, and Proteins by
... Molecular recognition is the process by which one molecule associates with another molecule via specific noncovalent interactions. The specificity of these interactions allows molecules to assemble in manner that is predetermined by their structural attributes, including size, shape, and polarity. I ...
... Molecular recognition is the process by which one molecule associates with another molecule via specific noncovalent interactions. The specificity of these interactions allows molecules to assemble in manner that is predetermined by their structural attributes, including size, shape, and polarity. I ...
Translation is simply the decoding of nucleotide sequences on
... transfer of the first amino acid at P site to the aminoacyl tRNA at the A site of the ribosome, forming a dipeptide tRNA at this position, and leaving the uncharged initiator tRNA at the P site. The next step in elongation is translocation, which requires another elongation factor known as transloca ...
... transfer of the first amino acid at P site to the aminoacyl tRNA at the A site of the ribosome, forming a dipeptide tRNA at this position, and leaving the uncharged initiator tRNA at the P site. The next step in elongation is translocation, which requires another elongation factor known as transloca ...
Chapter 3 The Same 20 Amino Acids Serve as Building Blocks for
... chains) exist predominantly as dipolar ions, known as zwitterions (German for “hybrid ions”). ...
... chains) exist predominantly as dipolar ions, known as zwitterions (German for “hybrid ions”). ...
A chitin-binding domain in a marine bacterial
... To examine the chitinases produced by these clones in further detail, crude protein extracts were prepared from IPTGinduced cells as described before (Svitil et al., 1997). Briefly, sonicated cells were incubated with sarcosyl(O1YO,w/v, final concentration) for 1h on ice, then centrifuged to remove ...
... To examine the chitinases produced by these clones in further detail, crude protein extracts were prepared from IPTGinduced cells as described before (Svitil et al., 1997). Briefly, sonicated cells were incubated with sarcosyl(O1YO,w/v, final concentration) for 1h on ice, then centrifuged to remove ...
Sequence and evolutionary analysis of the human trypsin subfamily
... and so forth (with the letter ‘‘P’’ being used for a clan containing families of more than one of the catalytic types serine, threonine and cysteine). Some clans are divided into ‘‘subclans’’ because there is evidence of a very ancient divergence within the clan, for example MA(E), the gluzincins, a ...
... and so forth (with the letter ‘‘P’’ being used for a clan containing families of more than one of the catalytic types serine, threonine and cysteine). Some clans are divided into ‘‘subclans’’ because there is evidence of a very ancient divergence within the clan, for example MA(E), the gluzincins, a ...
Protein Structure Prediction with Lattice Models
... bone, and blood. Membrane proteins are found in cells’ membranes, where they mediate the exchange of molecules and information across cellular boundaries. Water-soluble globular proteins serve as enzymes that catalyze most cellular biochemical reactions. Amino acids are joined end-to-end during prot ...
... bone, and blood. Membrane proteins are found in cells’ membranes, where they mediate the exchange of molecules and information across cellular boundaries. Water-soluble globular proteins serve as enzymes that catalyze most cellular biochemical reactions. Amino acids are joined end-to-end during prot ...
LatFit - Manual - Bioinformatics Group Freiburg
... in Protein Data Bank (PDB) format into a coarse grained lattice model representation. This is done by 1. fitting the Cα -atoms to neighbored lattice positions for representation of the protein backbone. 2. If side chains are modelled, Cβ or the center of mass of the residues are fitted to neighbored ...
... in Protein Data Bank (PDB) format into a coarse grained lattice model representation. This is done by 1. fitting the Cα -atoms to neighbored lattice positions for representation of the protein backbone. 2. If side chains are modelled, Cβ or the center of mass of the residues are fitted to neighbored ...
Insect Biochemistry 15:
... Saturated ammonium sulphate was then added to the supernatant to bring the saturation to 55'!,,. Alter stirring for 30 rain, the solution was centrifuged at 20,000g and the supernatant discarded. The pellet was dissolved in doublestrength buffer (1-2 ml). Removal of salt. The redissolved pellet was ...
... Saturated ammonium sulphate was then added to the supernatant to bring the saturation to 55'!,,. Alter stirring for 30 rain, the solution was centrifuged at 20,000g and the supernatant discarded. The pellet was dissolved in doublestrength buffer (1-2 ml). Removal of salt. The redissolved pellet was ...
A conserved N-‐terminal domain of Rif2 regulates telomere length in
... All of the S. cerevisiae strains (termed yeast strains) and oligonucleotides (termed primers) for PCR (Polymerase Chain Reaction) and for construction of plasmids used in this study are listed in the suppl ...
... All of the S. cerevisiae strains (termed yeast strains) and oligonucleotides (termed primers) for PCR (Polymerase Chain Reaction) and for construction of plasmids used in this study are listed in the suppl ...
Valproate
... Valproate (Epilim/Depakote). At therapeutic concentrations, >90% of Valproate is protein bound, mainly to albumin. Binding is saturable and at high valproate concentrations, low albumin levels and in hepatic disease the free fraction increases. Other compounds can compete for Valproate binding to al ...
... Valproate (Epilim/Depakote). At therapeutic concentrations, >90% of Valproate is protein bound, mainly to albumin. Binding is saturable and at high valproate concentrations, low albumin levels and in hepatic disease the free fraction increases. Other compounds can compete for Valproate binding to al ...
British Journal of Dairy Sciences 3(2): 9-13, 2013
... phenylalanine while the non-essential amino acids detected were arginine, aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine and tyrosine. The results show that the seventeen amino acids tested were present in all the samples in various amounts. For all the amino acids tested, ...
... phenylalanine while the non-essential amino acids detected were arginine, aspartic acid, serine, glutamic acid, proline, glycine, alanine, cysteine and tyrosine. The results show that the seventeen amino acids tested were present in all the samples in various amounts. For all the amino acids tested, ...
Isolation of a Strong Suppressor of Nonsense Mutations in Bacillus
... described [I 51. The irradiated bacteria were resuspended, at 5 x lo8 cells/ml, in minimal medium containing 0.5 mM amino acids, except leucine which was 0.01 mM, infected with phage 4 2 9 or with different sus mutants at a multiplicity of 20 and shaken at 37 "C. A control was kept uninfected. At 15 ...
... described [I 51. The irradiated bacteria were resuspended, at 5 x lo8 cells/ml, in minimal medium containing 0.5 mM amino acids, except leucine which was 0.01 mM, infected with phage 4 2 9 or with different sus mutants at a multiplicity of 20 and shaken at 37 "C. A control was kept uninfected. At 15 ...
Biochemistry 499
... Abnormal phosphorylation has been known as a cause or consequence of many human diseases, such as cancer. This stresses the importance of regulation of the enzymes involved in reversible phosphorylation, especially the protein phosphatases. There are relatively few Ser/Thr phosphatases in the cell a ...
... Abnormal phosphorylation has been known as a cause or consequence of many human diseases, such as cancer. This stresses the importance of regulation of the enzymes involved in reversible phosphorylation, especially the protein phosphatases. There are relatively few Ser/Thr phosphatases in the cell a ...
Journal of Bacteriology
... and B. J. J. Lugtenberg, J. Bacteriol. 174:214–221, 1992). We observed that genes homologous to ropA are present in strain 248 as well as in other R. leguminosarum strains, and we describe the cloning and characterization of two of these genes. Sequencing of a 2.2-kb BglII fragment from R. leguminos ...
... and B. J. J. Lugtenberg, J. Bacteriol. 174:214–221, 1992). We observed that genes homologous to ropA are present in strain 248 as well as in other R. leguminosarum strains, and we describe the cloning and characterization of two of these genes. Sequencing of a 2.2-kb BglII fragment from R. leguminos ...
Guidelines for the Safety Assessment of Foods Derived
... identified during the safety assessment process. Information and data submitted will be for the parts of the plant used as a food source as identified in 6.2.1 ...
... identified during the safety assessment process. Information and data submitted will be for the parts of the plant used as a food source as identified in 6.2.1 ...
The Computational Complexity of Protein
... bone, and blood. Membrane proteins are found in cells’ membranes, where they mediate the exchange of molecules and information across cellular boundaries. Water-soluble globular proteins serve as enzymes that catalyze most cellular biochemical reactions. Amino acids are joined end-to-end during prot ...
... bone, and blood. Membrane proteins are found in cells’ membranes, where they mediate the exchange of molecules and information across cellular boundaries. Water-soluble globular proteins serve as enzymes that catalyze most cellular biochemical reactions. Amino acids are joined end-to-end during prot ...
Backbone sequential assigment tutorial
... Specific View options of each spectrum can be accessed by the shortcut vt or View -> View options. The menu can also be accessed by right-clicking inside the spectrum. Contour levels can be adjusted by the shortcut ct or View -> More -> Contours Levels. These levels do not have to be changed anymore ...
... Specific View options of each spectrum can be accessed by the shortcut vt or View -> View options. The menu can also be accessed by right-clicking inside the spectrum. Contour levels can be adjusted by the shortcut ct or View -> More -> Contours Levels. These levels do not have to be changed anymore ...
intro 1 - Anderson Research
... assimilation, with 90% isolated ion exchangewhey protein, characterized by a mix of high biological value amino acids. It is able to meet the needs of sport professionals, both in the definition and muscle-increase phase. The sophisticated ion exchange technique has more than 90% protein content at ...
... assimilation, with 90% isolated ion exchangewhey protein, characterized by a mix of high biological value amino acids. It is able to meet the needs of sport professionals, both in the definition and muscle-increase phase. The sophisticated ion exchange technique has more than 90% protein content at ...
Two-hybrid screening
Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.