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microbiology exam i - Medical Mastermind Community
microbiology exam i - Medical Mastermind Community

... D. Mix together M. tuberculosis luciferase phage and a temperate phage specific for the new bacterial species and infect the new bacterial species. 28. You would like to design a diagnostic test for the 3 base-pair deletion that causes cystic fibrosis. What would be a reasonable method and efficient ...
A Conserved Family of Nuclear Proteins Containing
A Conserved Family of Nuclear Proteins Containing

Chapter 19_CHEM 131
Chapter 19_CHEM 131

... • Simple proteins contain only amino acid residues. • Conjugated proteins contain amino acid residues and other organic or inorganic components (prosthetic groups). ...
CH 908: Mass Spectrometry Lecture 9 Electron Capture Dissociation
CH 908: Mass Spectrometry Lecture 9 Electron Capture Dissociation

... • While exciting, these are often very difficult experiments, requiring a lot of time and good control experiments • The data one gets is often ambiguous, but no more so than solid state structures obtained in X-Ray crystallography • It’s crucial to differentiate between solution state and gas phase ...
RNA Synthesis and Splicing
RNA Synthesis and Splicing

... Transcription and Translation in Prokaryotes and Eukaryotes ...
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Genetic Coding in Ce..

... •Are made up of 2 subunits, a large one and a smaller one, each subunit contains ribosomal RNA (rRNA) & proteins. •Protein synthesis starts when the two subunits bind to mRNA. •The initiator codon AUG binds to the first anticodon of tRNA, signaling the start of a protein. ...
HM2019 - Hycult Biotech
HM2019 - Hycult Biotech

... The monoclonal antibody 67D3 recognizes human heart-type fatty acid-binding protein (H-FABP) of both natural and recombinant origin. The H-FABP protein is derived from the human FABP3 gene. FABPs are small intracellular proteins (~13-14 kDa) with a high degree of tissue specificity that bind long ch ...
Extended information on Western blot quantification To Gassen et al
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Transcription and Translation
Transcription and Translation

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... • All people are different • but the DNA of different people only varies for 0.2% or less • So, only up to 2 letters in 1000 are expected to be different. • Evidence in current genomics studies (Single Nucleotide Polymorphisms or SNPs) imply that • on average only 1 letter out of 1400 is different b ...
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... The monomers of Proteins • The building block of any protein is the amino acid. • There are 20 A.A’s and we only make 12 of them. (where do we get the rest?) • has an amino end (NH2) and a carboxyl end (COOH). ...
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... 20. ___________________________ (rRNA) binds to the mRNA and uses the instructions to assemble amino acids in the correct order 21. ___________________________ (tRNA) is the supplier. Transfer RNA delivers amino acids to the ribosome to be assembled into a protein 22. In the nucleus, enzymes make a ...
Sagri Eftymia
Sagri Eftymia

... through bioinformatics analysis. In Table IΙ the male and female differentially expressed proteins are shown. These proteins belong to different functional groups such as cytoskeleton, metabolism, synaptosomal, signal transduction and oxidative stress. Moreover, a new peptidomic approach was applied ...
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... The problem addressed here is to find potent and cancer-selective inhibitors of prevalidated cancer drug targets. We have chosen to find inhibitors of the MAP3K TAO2 because its elimination in a NSCLC (non-small-cell lung carcinoma) cell line killed the cells. TAO2 has been shown to participate in t ...
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... • accounting for 17.3 million deaths per year • a number that is expected to grow to >23.6 million by 2030 http://www.who.int/cardiovascular_diseases/en/ ...
PROTEIN EXTRACTION AND PURIFICATION
PROTEIN EXTRACTION AND PURIFICATION

Bioinformatics - University of Hawaii
Bioinformatics - University of Hawaii

... expression, proteomics is, in effect, the “product” science made possible by bioinformatics A proteome is the collection of all proteins expressed in a cell at a given time Every organism has 1 genome, but many proteomes In addition to “high throughput” protein analysis, proteomics is researched thr ...
The Process Whereby Your Genes Make Your Proteins
The Process Whereby Your Genes Make Your Proteins

... carboxyl group (COOH or COO-), which is what makes a compound an acid (therefore the name “amino acid”). Note also that they all also have a side chain (also called an organic group, an R group, or a functional group), and that each amino acid’s side chain is different from the other amino acids’ si ...
CHAPTER 17 FROM GENE TO PROTEIN
CHAPTER 17 FROM GENE TO PROTEIN

...  The discovery of ribozymes rendered obsolete the idea that all biological catalysts are proteins. Introns may play a regulatory role in the cell.  Specific functions have not been identified for most introns, but some contain sequences that regulate gene expression, and many affect gene products ...
secstruct_PT
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... example, in drug design) and biotechnology (for example, in the design of novel enzymes) ...
Ch 3 The Molecules of Cells
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Viewpoint - Prof Ralf Metzler
Viewpoint - Prof Ralf Metzler

... the fundamental processes of gene regulation, yet we know that genetic systems may be extremely stable. Facilitated diffusion—the interplay of three-dimensional and one-dimensional search of DNA binding proteins for their specific binding sites—has been proposed to explain the regulation speed and l ...
protein digest.99
protein digest.99

... in duodenum and move into capillary beds to portal vein ...
Collapse of Homochirality of Amino Acids in Proteins from Various
Collapse of Homochirality of Amino Acids in Proteins from Various

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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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