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Slides/AVS 504 Met Fri 2013 pt 2
Slides/AVS 504 Met Fri 2013 pt 2

Tertiary structure
Tertiary structure

... polypeptide chain is called a "subunit." The way these chains fit together or associate with one another is referred to as the "quaternary structure." • The quarternary structure of the protein refers to the way multiple subunits of a protein interact. This is the arrangement of the individual subun ...
Ch 5 Macromolecules
Ch 5 Macromolecules

... Considering the chemical characteristics of the amino acids valine and glutamic acid, propose a possible explanation for the dramatic effect on the protein function that occurs when valine is substituted for glutamic acid. The R group on glutamic acid is acidic and hydrophilic, wheras the R group o ...
Alpha/Beta structures
Alpha/Beta structures

... residues in the inside of barrel • Coenzyme A (green) binds in the barrel ...
Macromolecules Review ws Name the 6 main elements that make
Macromolecules Review ws Name the 6 main elements that make

... which can join together to make a protein. 17. Phosholipids makes up cell membranes. 18. Fats are made of an alcohol called glycerol and three fatty acids chains. This is known as a triglyceride 19. If there are all SINGLE bonds between carbons in the fatty acid chain, then it is said to be saturate ...
Gene Section GDF15 (growth differentiation factor 15) Atlas of Genetics and Cytogenetics
Gene Section GDF15 (growth differentiation factor 15) Atlas of Genetics and Cytogenetics

... 308 amino acids that contain a 29 amino acid signal peptide, a 167 amino acid propeptide, and a 112 amino acid mature protein. The mature protein is secreted as a homodimer linked by disulfide bonds and is released from the propeptide following intracellular cleavage at RXXR furine-like cleavage sit ...
1 Introduction 2 Central Dogma of molecular biology 3 DNA
1 Introduction 2 Central Dogma of molecular biology 3 DNA

... base and the 5’ position a phosphate group. The sugar in DNA is de-oxyribose and that’s why the name de-oxyribonucleotide. DNA’s nucleotides are of two types: purines (A and G) and pyrimidines (C and T). They differ in the base. Nucleotides are connected using the phosphate group of one nucleotide w ...
Bacterial Rhodopsin Light-driven Proton Pump
Bacterial Rhodopsin Light-driven Proton Pump

... 2. This causes pKa changes in the Schiff’s base and amino acid residues. Sequential conformational changes  differential access to the two half channels (each step is fast but has its own characteristic half life). 3. These changes allow access of the Schiff’s base (SB) to protonation only from the ...
What are mitochondria? Mitochondria are the
What are mitochondria? Mitochondria are the

... At the high school level, we worked with Shad Valley, a well-known Canadian not-for-profit educational organization, to produce workshops aimed specifically on synthetic biology and problem solving through real life applications. We presented a forensic case based on synthetic biology, as well as te ...
Kinases
Kinases

... Assuming that each branch point represented a gene duplication event, the immediate ancestral gene as it was at the time of duplication was given a name (fig 1) and a sequence was determined as a consensus sequence of its progeny using its nearest neighbour as an outgroup to determine which amino ac ...
FERMENTATION: an anaerobic biological reaction process in which
FERMENTATION: an anaerobic biological reaction process in which

... a period of rest ...
Proteins
Proteins

...  In the context of a protein… • Oxygen tends to exhibit a slight negative charge • Nitrogen tends to exhibit a slight positive charge • Carbon tends to remain neutral/uncharged ...
Proteins
Proteins

...  In the context of a protein… • Oxygen tends to exhibit a slight negative charge • Nitrogen tends to exhibit a slight positive charge • Carbon tends to remain neutral/uncharged ...
SAM Teacher`s Guide Four Levels of Protein Structure - RI
SAM Teacher`s Guide Four Levels of Protein Structure - RI

Food Chemistry
Food Chemistry

... foods is partly dependent on chemical reactions occurring during processing and storage. If proteins are overheated, the amino acid structure changes. As a result, the protein is not easily digested or absorbed. Savoie, Charbonneau, and Parent (1989) reported that overheating of non-fat dried milk, ...
sc-33290 (Page 1) - Santa Cruz Biotechnology, Inc.
sc-33290 (Page 1) - Santa Cruz Biotechnology, Inc.

... The chromatin structure remodeling complex, or RSC, is involved in cell growth in S. cerevisiae. Nuclear protein STH1/NPS1, or STH1, is a part of the RSC complex and is an ATPase. Single stranded DNA stimulates the ATPase activity of STH1 and subsequent nucleosome remodeling. STH1 is also necessary ...
A candidate gene marker for bloat susceptibility in cattle?
A candidate gene marker for bloat susceptibility in cattle?

... 1991). We have found that the abundance of a major salivary protein, which we have termed bovine salivary protein 30 kDa (bSP30) is increased on average by 66 ±15% in animals from the low susceptibility compared with the high susceptibility herd (Rajan et al., 1996). Here we report the identificatio ...
Slide 1
Slide 1

... 300 – 500 nucleotides to nascent RNA chains. Very little new initiation occurs. By hybridizing the labeled RNA to the cloned DNA for a specific gene (A in this case), the fraction of total RNA produced from that gene (i.e., its relative transcription rate) can be measured. [See J. Weber et al., 1977 ...
doc Final Exam 2002
doc Final Exam 2002

... 23. The enzyme protein disulphide isomerase (PDI) catalyzes a reaction that a) isomerizes glutathione reductase b) stabilizes protein conformation in the ER c) stabilizes protein conformation in the cytoplasm d) reduces cysteine disulphide bridges e) oxidizes cysteine disulphide bridges 24. If a per ...
Protein Synthesis PP
Protein Synthesis PP

...  This order is called the reading frame  Changing the reading frame completely changes the resulting ...
Towards the atomic level protein sequence analysis
Towards the atomic level protein sequence analysis

... class of channel protein that controls the precise transport of water molecules across the cell membranes in all the living organisms was done. The aquaporins are thus likely to be of fundamental significance to all facets of plant growth and developments, affected by plant–water relations. A majori ...
Max ARM (Anabolic Recovery Matrix) from Max Muscle Sports
Max ARM (Anabolic Recovery Matrix) from Max Muscle Sports

... Max ARM (Anabolic Recovery Matrix) from Max Muscle Sports Nutrition (MMSN) was designed to follow rapid recovery and anabolic science. Created to be the ultimate post-workout formula, Max ARM provides the necessary nutrients to support maximum muscle mass activation, restore fluids and electrolytes, ...
presentation source
presentation source

... fact spent on onstructing the scaffold of this map. In contrast, Celera Genomics allegedly used an approach called shotgun sequencing that works by randomly cutting up the genome into small streches, sequencing them, and then using a clever algorithm to assemble the whole genome. There was much deba ...
2009 Dental Biochemistry (Questions)
2009 Dental Biochemistry (Questions)

... C) this leads to the production of glutamine in the kidney, which is the way humans generate energy in the absence of adequate intake of proteins. D) glutamate can undergo a reaction that produces ammonia that can, in turn, enter the urea cycle. E) this becomes a signal to up regulate the urea cycle ...
Chapter 3 Topic: Biomolecules Main concepts: •In chemistry, the
Chapter 3 Topic: Biomolecules Main concepts: •In chemistry, the

... when the helix or pleated sheet folds back on itself to form twisted or knot-like structures. This is caused by hydrogen bonding, and special bonds called disulfide bridges that form between amino acids. Quaternary structure is where multiple proteins are linked together into one large structure. He ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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