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FoldNucleus: web server for the prediction of RNA
FoldNucleus: web server for the prediction of RNA

... free energy landscape. Because the function of RNA depends on its conformation, which is analogous to the relationship between the function and folding structure of proteins, researchers have successfully applied methods developed for proteins, such as the A analysis (Matouschek et al., 1990). In th ...
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Protein - people.vcu.edu
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ANALYTICAL BIOCHEMISTRY
ANALYTICAL BIOCHEMISTRY

... theoretical principles and techniques underlying the full range of clinical biochemical analyses. Emphasis should be placed upon the factors which govern the choice of method and on the evaluation of instruments and methods. The headings and subsections mentioned below indicate those methods, instru ...
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Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
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