• Study Resource
  • Explore Categories
    • Arts & Humanities
    • Business
    • Engineering & Technology
    • Foreign Language
    • History
    • Math
    • Science
    • Social Science

    Top subcategories

    • Advanced Math
    • Algebra
    • Basic Math
    • Calculus
    • Geometry
    • Linear Algebra
    • Pre-Algebra
    • Pre-Calculus
    • Statistics And Probability
    • Trigonometry
    • other →

    Top subcategories

    • Astronomy
    • Astrophysics
    • Biology
    • Chemistry
    • Earth Science
    • Environmental Science
    • Health Science
    • Physics
    • other →

    Top subcategories

    • Anthropology
    • Law
    • Political Science
    • Psychology
    • Sociology
    • other →

    Top subcategories

    • Accounting
    • Economics
    • Finance
    • Management
    • other →

    Top subcategories

    • Aerospace Engineering
    • Bioengineering
    • Chemical Engineering
    • Civil Engineering
    • Computer Science
    • Electrical Engineering
    • Industrial Engineering
    • Mechanical Engineering
    • Web Design
    • other →

    Top subcategories

    • Architecture
    • Communications
    • English
    • Gender Studies
    • Music
    • Performing Arts
    • Philosophy
    • Religious Studies
    • Writing
    • other →

    Top subcategories

    • Ancient History
    • European History
    • US History
    • World History
    • other →

    Top subcategories

    • Croatian
    • Czech
    • Finnish
    • Greek
    • Hindi
    • Japanese
    • Korean
    • Persian
    • Swedish
    • Turkish
    • other →
 
Profile Documents Logout
Upload
Compartmentation in plant metabolism
Compartmentation in plant metabolism

... about compartmentation of enzymes and other proteins in plant cells, but current technologies do have some limitations. Whatever the approach used to identify proteins, the reliability of the results is absolutely dependent on the purity of the original organelle preparation, and the level of contam ...
hydrolysis of keratin materials derived from poultry industry
hydrolysis of keratin materials derived from poultry industry

... consumption of poultry meat grows year by year, which results in an increase of its production. Feathers consist in 90% of keratin and they make up 5-7% of the total weight of adult chickens what causes that feathers waste is the main source of keratin. Feathers are discarded in the process of the c ...
1st Sem (unit I)
1st Sem (unit I)

... participate in H-bonding. Their side chains are hydrophobic and generally fill up the interior of the folded protein . They are 9 and include glycine, alanine , valine, leucine, isoleucine, phenylalnine, tryptophan, methionine and proline b) Amino acids with Uncharged polar side chains:These amino a ...
Unzipping Genes - HiMedia Laboratories
Unzipping Genes - HiMedia Laboratories

... HiMedia’s RDP TrioTM Reagent is designed for rapid purification of RNA, DNA and Protein from different samples. This product which is a mixture of guanidine thiocyanate and phenol in a mono-phase solution effectively dissolves RNA. After adding chloroform and centrifuging, the mixture separates into ...
Taxonomy, biology and physiology of fungi
Taxonomy, biology and physiology of fungi

... Obligately oxidative. Obligate aerobes. Exp. Rhodotorula Facultatively fermentative. Energy can be obtained by oxidative and fermentative processed such fungi are likely to be faculative anaerobes. Oxidative metabolism, provides much more energy than fermentative, so higher yields can occur under ae ...
Cellular Mechanisms
Cellular Mechanisms

... Non Competitive inhibitors • Non Competitive Inhibitors (two types) – Reversible bind non-covalently, reversibly to the enzyme • Alter conformation of enzyme • Not at the active site, not competed out by ...
Fruitful Collaboration of Physician/Physiologist and Engineer in the
Fruitful Collaboration of Physician/Physiologist and Engineer in the

... the importance of defining systems in the modeling process. They also illustrate the use of structured problem solving, novel experimental design, and data analysis techniques. ...
Bacterial Transformation with Green Fluorescent Protein
Bacterial Transformation with Green Fluorescent Protein

... pest, or drought resistance can be genetically transformed into plants. Bacteria can be genetically  transformed with genes enabling them to digest oil spills. Diseases caused by defective genes are  beginning to be treated by transforming a sick person's cells with healthy copies of the defective g ...
Managing people in sport organisations
Managing people in sport organisations

... Translation. A molecule of transfer RNA (tRNA) charged with its specific amino acid, phenylalanine, and already linked to the growing peptide chain, is positioned on the mRNA by complementary pairing of triplet of nucleotides with its codon of three nucleotides in the mRNA. A second molecule of tRNA ...
Theory_2004
Theory_2004

Drug delivery systems based on sugar
Drug delivery systems based on sugar

... on hepatocyte surfaces (50,000 to 500,000 per cell) [9]. In addition to lectin receptors that are regularly involved in endocytosis, those that are not may also be targeted. For example, lectin-like 'homing' receptors on lymphocytes recognize so-called cell adhesion molecules (CAM) that contain carb ...
Darnell, JC, Warren, ST and Darnell, RB: The fragile X mental retardation protein, FMRP, recognizes G-quartets. Mental Retardation and Developmental Disabilities Research Reviews 10:49-52 (2004).
Darnell, JC, Warren, ST and Darnell, RB: The fragile X mental retardation protein, FMRP, recognizes G-quartets. Mental Retardation and Developmental Disabilities Research Reviews 10:49-52 (2004).

... X patients who express no FMRP) would provide an important measure of their likelihood as legitimate FMRP target RNAs. In fact, analysis of polysomes prepared from the above cell lines led to the finding that 10 of 13 candidate FMRP RNA targets showed alteration in their polysome distribution in the ...
In the light of directed evolution: Pathways of adaptive protein evolution
In the light of directed evolution: Pathways of adaptive protein evolution

... beneficial, and so presumably could have also been discovered separately with lower mutation rates. The procedure used to identify improved mutants depends on the details of the particular protein and engineering goal. In some cases, the protein property of interest can be coupled to the survival of ...
MB ChB PHASE I
MB ChB PHASE I

... chymotrypsin; carboxypeptidases A and B. ...
Np73 is capable of inducing apoptosis by co
Np73 is capable of inducing apoptosis by co

... transcription on its own [6–10], although the mechanisms are still not fully understood. Liu et al. [7] described that the 13 unique Nterminal residues of Np73-β, together with the adjacent PXXP motifs, constitute a novel TA domain, mediating the capacity of the isoform to suppress cell growth and ...
Full-Text PDF
Full-Text PDF

... phospholipid bilayer. Individually, such noncovalent bonds are weak, but the frequency at which the methyl groups and ester carbonyl oxygens repeat along the cPHB backbone allows each polymer molecule to have multiple interactions. The number and strength of these bonds would be determined by the pr ...
GRE BIOCHEMISTRY TEST PRACTICE BOOK
GRE BIOCHEMISTRY TEST PRACTICE BOOK

... The content of the test is organized into three major areas: biochemistry, cell biology, and molecular biology and genetics. In addition to the total score, a subscore in each of these subfield areas is reported. Because these three disciplines are basic to the study of all organisms, test questions ...
Amino acids, introduction
Amino acids, introduction

Synthetic Peptides as Antigens for Antibody Production
Synthetic Peptides as Antigens for Antibody Production

... include the Turn scales of Pellequer and Westhof (4). These are based on the occurrence of amino acids within turns. The level of correctly predicted antigenicity using this program is high (70%), but the number of predicted antigenic sites per protein is smaller than for other programs. In general, ...
Distribution in percentage Inter-environmental
Distribution in percentage Inter-environmental

...  Pigments analysis (differentiate phylum only–takes years )  Sequencing a biomarker of diversity (e.g., 16S/18S)  Very sensitive: detect thousands of lowly abundant taxa  Want to know the functionality of a microbial community?  Sequence all the extracted DNA and annotate reads  Want to know ...
Slide 1
Slide 1

... In a polypeptide there is always one end with a free amino (NH3) group, called the N-terminus, and one end with a free carboxyl (CO2) group, called the C-terminus. ...
Biochemical Evidence for the Role of the Waxy Protein fron Pea
Biochemical Evidence for the Role of the Waxy Protein fron Pea

Characterization of Saccharomyces cerevisiae deficient in
Characterization of Saccharomyces cerevisiae deficient in

... However, it should be noted that the Ricinus and putative yeast PLDs differ considerably in their overall size (95 as against 195 kDa) and in other regions of their sequence. The only form of PLD purified to homogeneity from a mammalian source was shown to migrate with a molecular size of 195 kDa on ...
characterization of proteins from the cytoskeleton of giardia lamblia
characterization of proteins from the cytoskeleton of giardia lamblia

... with tubulin; by selecting appropriate subunits from among tubulin species; or, after incorporation of common tubulin precursors, by allowing chemical modification of dimers in organelle- or site-specific patterns. Giardia is an endoparasitic flagellate with a number of microtubular organelles. Extr ...
Bioinformatics approaches for functional
Bioinformatics approaches for functional

... functions of membrane proteins have been investigated with several computational approaches, such as developing databases, analyzing the structure ^ function relationship and establishing algorithms to discriminate different type of membrane proteins. However, compilation of bioinformatics resources ...
< 1 ... 127 128 129 130 131 132 133 134 135 ... 466 >

Two-hybrid screening



Two-hybrid screening (also known as yeast two-hybrid system or Y2H) is a molecular biology technique used to discover protein–protein interactions (PPIs) and protein–DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively.The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay.
  • studyres.com © 2026
  • DMCA
  • Privacy
  • Terms
  • Report