DEVELOPMENT AND CHARACTERIZATION OF A TRANSGENIC
... situ hybridization provides a basis for understanding the normal function of PVR, which is a novel member of the immunoglobulin superfamily of proteins. To characterize the tropism of poliovirus infection in PVR transgenic mice, poliovirus replication sites were examined by in situ hybridization. Th ...
... situ hybridization provides a basis for understanding the normal function of PVR, which is a novel member of the immunoglobulin superfamily of proteins. To characterize the tropism of poliovirus infection in PVR transgenic mice, poliovirus replication sites were examined by in situ hybridization. Th ...
genetics and functions of herpes simplex virus type 1 membrane
... Table 4.3: Differential effects of mutations on infectious virion production and cell fusion ....................................................................................................197 ...
... Table 4.3: Differential effects of mutations on infectious virion production and cell fusion ....................................................................................................197 ...
Dissertation zur Erlangung des Doktorgrades der Fakultät für Chemie und Pharmazie
... the appropriate cellular milieu (expression of stress response genes) required for AAV DNA replication than direct involvement of helper virus gene products (Yakobson et al. 1987). The natural route for AAV infections is assumed to occur via the respiratory or gastrointestinal route as is the case f ...
... the appropriate cellular milieu (expression of stress response genes) required for AAV DNA replication than direct involvement of helper virus gene products (Yakobson et al. 1987). The natural route for AAV infections is assumed to occur via the respiratory or gastrointestinal route as is the case f ...
Assembly and budding of rabies virus
... SAD P 288AAA, displayed approx. 10,000‐100,000fold reduced cell‐free infectious titers but maintained the capacity to spread the infection from cell to cell. The specific defect in budding was verified by demonstrating that the function of P as polymerase cofactor and N binding were not af ...
... SAD P 288AAA, displayed approx. 10,000‐100,000fold reduced cell‐free infectious titers but maintained the capacity to spread the infection from cell to cell. The specific defect in budding was verified by demonstrating that the function of P as polymerase cofactor and N binding were not af ...
Adobe PDF - Office of Research Integrity
... animals. A number of features are incorporated in the latest vector designs to enhance biosafety. These features include: • Transgene: Non-oncogene; Vector and packaging components are distributed onto multiple plasmids that contain very little, if any, overlap or homology • Deletion of viral genes ...
... animals. A number of features are incorporated in the latest vector designs to enhance biosafety. These features include: • Transgene: Non-oncogene; Vector and packaging components are distributed onto multiple plasmids that contain very little, if any, overlap or homology • Deletion of viral genes ...
Orr M.T., K.H. Edelmann, J. Vieira, L. Corey, D.H. Raulet, and C.B. Wilson. 2005. Inhibition of MHC class I is a virulence factor in herpes simplex virus infection of mice. PLoS Pathog 1(1):e7.
... targets MHC class I antigen presentation by binding to the transporter associated with antigen presentation (TAP) 1/2 complex, preventing transport of peptides from the cytosol to the endoplasmic reticulum where peptides are loaded into the nascent MHC class I heavy-chain b2 microglobulin (b2m) comp ...
... targets MHC class I antigen presentation by binding to the transporter associated with antigen presentation (TAP) 1/2 complex, preventing transport of peptides from the cytosol to the endoplasmic reticulum where peptides are loaded into the nascent MHC class I heavy-chain b2 microglobulin (b2m) comp ...
Chapter 2 – Understanding the human body and the immune system
... such as the thigh bone (femur) also make blood cells. ...
... such as the thigh bone (femur) also make blood cells. ...
Herpes simplex virus latency-associated transcript gene function
... the LATs play a role in promoting the efficient establishment of latency (Sawtell and Thompson, 1992; Thompson and Sawtell, 1997). Although a number of earlier studies showed that mutations in the LAT region had no effect on the level of latent viral DNA (Sederati et al, 1989), several recent report ...
... the LATs play a role in promoting the efficient establishment of latency (Sawtell and Thompson, 1992; Thompson and Sawtell, 1997). Although a number of earlier studies showed that mutations in the LAT region had no effect on the level of latent viral DNA (Sederati et al, 1989), several recent report ...
2 INTRODUCTION VIRUSES Viruses are subcellular, infectious
... replication to form a ribonucleoprotein replication complex associated with an ERderived membranous web [14]. These RNA copies are then able to enter to the cycle again, producing more HCV proteins. The HCV structural proteins (C, E1 and E2), along with copies of HCV RNA, are packaged as infectious ...
... replication to form a ribonucleoprotein replication complex associated with an ERderived membranous web [14]. These RNA copies are then able to enter to the cycle again, producing more HCV proteins. The HCV structural proteins (C, E1 and E2), along with copies of HCV RNA, are packaged as infectious ...
innate, adaptive and intrinsic immunity in human immunodeficiency
... macrophages, which was associated with HIV disease progression (Nicol et al., 2008). In the study performed by Wu et al. (2012) the detection of the down-regulation of the enrichment genes along the MAPK branch not only fully supported the previous observations on altered expressions of TLR2/4, ERK/ ...
... macrophages, which was associated with HIV disease progression (Nicol et al., 2008). In the study performed by Wu et al. (2012) the detection of the down-regulation of the enrichment genes along the MAPK branch not only fully supported the previous observations on altered expressions of TLR2/4, ERK/ ...
Detection of bluetongue virus and African horseslckness virus in co
... sensitive than DNA probes, a positive- and a negative-sense single-stranded RNA (ssRNA) and a DNA NS1 gene probe in the in situ dot-spot hybridization procedure were compared. It was considered that there was no advantage in using ssRNA probes. The sensitivity of the negative-sense ssRNA was the sam ...
... sensitive than DNA probes, a positive- and a negative-sense single-stranded RNA (ssRNA) and a DNA NS1 gene probe in the in situ dot-spot hybridization procedure were compared. It was considered that there was no advantage in using ssRNA probes. The sensitivity of the negative-sense ssRNA was the sam ...
Influence of insertion site of the avian influenza virus haemagglutinin
... analysis. Total RNA was isolated from the virus-infected CEFs, separated in denaturing agarose gels, transferred to nylon membranes and hybridized with 32P-labelled antisense RNA specific for AIV H5, AIV N1 or NDV P. The signals were quantified by radioluminography using a FLA3000 scanner (Fujifilm) ...
... analysis. Total RNA was isolated from the virus-infected CEFs, separated in denaturing agarose gels, transferred to nylon membranes and hybridized with 32P-labelled antisense RNA specific for AIV H5, AIV N1 or NDV P. The signals were quantified by radioluminography using a FLA3000 scanner (Fujifilm) ...
Educator Materials Demonstration HIV Protease Inhibitors HIV
... Students may wonder how protease inhibitors relate to other drugs used to treat HIV. Other drugs are available that target different steps in the HIV life cycle: viral entry, reverse transcription, integration, and protein cleavage. The first such drug, azidothymidine, which inhibits the viral re ...
... Students may wonder how protease inhibitors relate to other drugs used to treat HIV. Other drugs are available that target different steps in the HIV life cycle: viral entry, reverse transcription, integration, and protein cleavage. The first such drug, azidothymidine, which inhibits the viral re ...
Viral Genomes
... that is very often encoded by these genomes is a RNA-dependent RNA polymerase (RdRp). These polymerases are essential for the replication of both positive and negative strand ssRNAs. as well as dsRNAs. This is true for both monopartite and multipartite RNA viruses that show a range of 1-13 proteins. ...
... that is very often encoded by these genomes is a RNA-dependent RNA polymerase (RdRp). These polymerases are essential for the replication of both positive and negative strand ssRNAs. as well as dsRNAs. This is true for both monopartite and multipartite RNA viruses that show a range of 1-13 proteins. ...
Impaired TLR3-dependent induction of IFN-a, -b,
... endosomal compartments • Ligands for TLR3: - dsRNA (Reovirus) - dsRNA intermediates (RNA, DNA viruses) - cellular RNA, released upon tissue damage (ssRNA, mRNA) • TLR3 is expressed abundantly in astrocytes, as well as in microglial cells and neurons upon viral infections and brain disorders ...
... endosomal compartments • Ligands for TLR3: - dsRNA (Reovirus) - dsRNA intermediates (RNA, DNA viruses) - cellular RNA, released upon tissue damage (ssRNA, mRNA) • TLR3 is expressed abundantly in astrocytes, as well as in microglial cells and neurons upon viral infections and brain disorders ...
43. Tumor Viruses
... Although it has been demonstrated that viral oncogenes can cause malignant transformation, it has not been directly shown that cellular oncogenes can do so. However, as described in Table 43–2, the following evidence suggests that they do: 1. DNA containing cellular oncogenes isolated from certain t ...
... Although it has been demonstrated that viral oncogenes can cause malignant transformation, it has not been directly shown that cellular oncogenes can do so. However, as described in Table 43–2, the following evidence suggests that they do: 1. DNA containing cellular oncogenes isolated from certain t ...
Haemophilus influenzae
... factors e.g. SP1, NF-kB Mediated through control regions in the 5’ LTR ...
... factors e.g. SP1, NF-kB Mediated through control regions in the 5’ LTR ...
Viral virulence genes
... Cannot compare virulence of different viruses For similar viruses, assays must be the same ...
... Cannot compare virulence of different viruses For similar viruses, assays must be the same ...
Lentivirus and Lentiviral Vectors
... All Principals Investigators (PI) who work with lentiviral vectors must have an approved IBC protocol including an SOP for lentiviral work and how to respond in case of exposure. All lab workers must be properly trained on safe work with lentivirus and emergency response. The current guidelines for ...
... All Principals Investigators (PI) who work with lentiviral vectors must have an approved IBC protocol including an SOP for lentiviral work and how to respond in case of exposure. All lab workers must be properly trained on safe work with lentivirus and emergency response. The current guidelines for ...
Common Characteristics and Distinct Features of Human
... Besides the virion structure and the length of the dsDNA genomes, human herpesviruses share further biological characteristics, which are important for the understanding of their infection and disease. Latency and recurrent infection: Upon primary infection, herpesviruses invariably establish a life ...
... Besides the virion structure and the length of the dsDNA genomes, human herpesviruses share further biological characteristics, which are important for the understanding of their infection and disease. Latency and recurrent infection: Upon primary infection, herpesviruses invariably establish a life ...
Introduction of green fluorescent protein into
... genome components that are essential for the replication of viral genome and the overproduction of mRNAs of the foreign gene. The transcribed RNA will not contain the gene for the structural proteins that are necessary for the production of virus particles (Schlesinger, et al., 1993). These are pro ...
... genome components that are essential for the replication of viral genome and the overproduction of mRNAs of the foreign gene. The transcribed RNA will not contain the gene for the structural proteins that are necessary for the production of virus particles (Schlesinger, et al., 1993). These are pro ...
Scientific American, June, 1997, 276
... stem cells, which persist indefinitely and also produce more specialized descendant cells. Blood-forming stem cells, for example, give rise to every other type of blood cell (red cells, white cells of the immune system, and so on) and reconstitute the blood as needed; they also make more copies of t ...
... stem cells, which persist indefinitely and also produce more specialized descendant cells. Blood-forming stem cells, for example, give rise to every other type of blood cell (red cells, white cells of the immune system, and so on) and reconstitute the blood as needed; they also make more copies of t ...
Active Immunisation and Vaccination
... becoming attached by glycoprotein on its surface to specific receptors on the helper T cells. The HIV particle fuses with the membrane of the helper T cell and the virus enters the cell. ...
... becoming attached by glycoprotein on its surface to specific receptors on the helper T cells. The HIV particle fuses with the membrane of the helper T cell and the virus enters the cell. ...