Mutations in the VPS45 gene, a SEC1 homologue, result in vacuolar
... similar to class A strains but have a large, single vacuolar structure and exhibit defects in mother-to-daughter cell vacuolar inheritance. Class E mutants contain a novel endosome-like compartment in addition to normal vacuoles, and class F mutants contain a single large vacuole structure that is e ...
... similar to class A strains but have a large, single vacuolar structure and exhibit defects in mother-to-daughter cell vacuolar inheritance. Class E mutants contain a novel endosome-like compartment in addition to normal vacuoles, and class F mutants contain a single large vacuole structure that is e ...
Biotechnology Laboratory (Kallas)
... (Synechococcus PCC 7002) and a photosynthetic electron transport mutant (PetB-R214H) that overproduces oxygen radicals and have these hybridized against the array. Most of the work would then involve exploration of computer programs for analysis of these data to pick out sets of genes that ...
... (Synechococcus PCC 7002) and a photosynthetic electron transport mutant (PetB-R214H) that overproduces oxygen radicals and have these hybridized against the array. Most of the work would then involve exploration of computer programs for analysis of these data to pick out sets of genes that ...
Coatomer Is Essential for Retrieval of Dilysine
... temperatures tested. Anterograde secretory transport was also assessed in these mutants by monitoring intracellular transport of carboxypeptidase Y (CPY). During transit through the secrelory pathway, CPY is converted from the ER precursor form (pl) to the Golgi-modified form (~2) and vacuolar form ...
... temperatures tested. Anterograde secretory transport was also assessed in these mutants by monitoring intracellular transport of carboxypeptidase Y (CPY). During transit through the secrelory pathway, CPY is converted from the ER precursor form (pl) to the Golgi-modified form (~2) and vacuolar form ...
SapkE
... • That cluster includes eight PKS subunits encoding genes sapkA, sapkB, sapkC, sapkD, sapkE, sapkF, sapkG, sapkH and 29 additional genes listed in the Table 1. and Fig.1. A. • In silico analysis showed that the full length PKS consists of 16 modules. All modules responsible for extension process has ...
... • That cluster includes eight PKS subunits encoding genes sapkA, sapkB, sapkC, sapkD, sapkE, sapkF, sapkG, sapkH and 29 additional genes listed in the Table 1. and Fig.1. A. • In silico analysis showed that the full length PKS consists of 16 modules. All modules responsible for extension process has ...
CD spectroscopy
... HSQC. The spectrum shows cross peaks between 15N and 1H in the NH group of the peptide bond, which thus creates one cross peak for every amino acid in the protein. In this picture the assignment of the spectrum is displayed onto the spectrum, which means that they have already found out which peak b ...
... HSQC. The spectrum shows cross peaks between 15N and 1H in the NH group of the peptide bond, which thus creates one cross peak for every amino acid in the protein. In this picture the assignment of the spectrum is displayed onto the spectrum, which means that they have already found out which peak b ...
Membrane Transport
... A. Carrier-mediated transport exhibits the properties of specificity, competition, and saturation. B. The transport rate of molecules such as glucose reaches a maximum when the carriers are saturated. This maximum rate is called the transport maximum, or T m. II. The transport of molecules such as g ...
... A. Carrier-mediated transport exhibits the properties of specificity, competition, and saturation. B. The transport rate of molecules such as glucose reaches a maximum when the carriers are saturated. This maximum rate is called the transport maximum, or T m. II. The transport of molecules such as g ...
Why and how do plants regulate their pH?
... • Does the ‘balance sheet’ for OH- & H+ add up, taking into account NAD & ATP? (Sakano 1998). It does as long as NADH & ATP are ‘recycled’, as in normal growth • The pH-stat relies on prior transport of H+ out of cells, plus C+ in, to ‘set up’ the organic acid/anion mixture (C+ not shown in diagramm ...
... • Does the ‘balance sheet’ for OH- & H+ add up, taking into account NAD & ATP? (Sakano 1998). It does as long as NADH & ATP are ‘recycled’, as in normal growth • The pH-stat relies on prior transport of H+ out of cells, plus C+ in, to ‘set up’ the organic acid/anion mixture (C+ not shown in diagramm ...
Slide 1
... FIGURE 2-2: Top row: Complex lipids interact with water and with each other to form different states of aggregation, or ‘phases’, shown here schematically. Open circles represent the more polar head groups, and dark lines and areas represent nonpolar hydrocarbon chains. The phase structures are gen ...
... FIGURE 2-2: Top row: Complex lipids interact with water and with each other to form different states of aggregation, or ‘phases’, shown here schematically. Open circles represent the more polar head groups, and dark lines and areas represent nonpolar hydrocarbon chains. The phase structures are gen ...
protein metabolism - cmb
... longer requires the assistance of the chaperone, whereas the remaining uncommitted (uc) molecules rebind and participate in another chaperone cycle. ...
... longer requires the assistance of the chaperone, whereas the remaining uncommitted (uc) molecules rebind and participate in another chaperone cycle. ...
Evolution of the Bacterial Flagellum
... and may have been derived from suba proton from outside to inside the bacterium or organelle which drives rotation of the c-subunit ring and ATP synthesis. The close relationship of mitochondrial and chloroplast units of rotary F-type ATPases. F-ATPase subunits with those of bacteria reflect the ori ...
... and may have been derived from suba proton from outside to inside the bacterium or organelle which drives rotation of the c-subunit ring and ATP synthesis. The close relationship of mitochondrial and chloroplast units of rotary F-type ATPases. F-ATPase subunits with those of bacteria reflect the ori ...
Application Note: Using the NanoDrop One to Quantify Protein and
... colorimetric assays, direct spectrophotometric UV measurements (such as A280), and amino acid analysis. All of these methods have their strengths and weaknesses. Direct spectrophotometric microvolume UV measurements are a popular choice for researchers because they are simple to perform, require no ...
... colorimetric assays, direct spectrophotometric UV measurements (such as A280), and amino acid analysis. All of these methods have their strengths and weaknesses. Direct spectrophotometric microvolume UV measurements are a popular choice for researchers because they are simple to perform, require no ...
Solid-State NMR Studies of the Structure of Membrane Bound Ras
... H-Ras and K-Ras are PM -associated proteins, which are ubiquitously expressed in mammalian cells. These highly homologous proteins interact in vitro with the same set of effectors but generate distinct signaling outputs in vivo [24]. K-Ras is a more potent activator of Raf-1 than H-Ras, but is a les ...
... H-Ras and K-Ras are PM -associated proteins, which are ubiquitously expressed in mammalian cells. These highly homologous proteins interact in vitro with the same set of effectors but generate distinct signaling outputs in vivo [24]. K-Ras is a more potent activator of Raf-1 than H-Ras, but is a les ...
Gene Ontology (GO)
... • Semiempirical potentials are derived from analytical expressions, describing the different interactions encountered in proteins, whose parameters are obtained by fitting experimental data on small molecules and/or from quantum mechanical calculations (Halgren, 1995 ; Moult, 1997 ; Lazaridis and Ka ...
... • Semiempirical potentials are derived from analytical expressions, describing the different interactions encountered in proteins, whose parameters are obtained by fitting experimental data on small molecules and/or from quantum mechanical calculations (Halgren, 1995 ; Moult, 1997 ; Lazaridis and Ka ...
Signal sequence peptides at an air-water interface
... (Blobel & Dobberstein, 1975). The primary sequences of signal sequence peptides exhibit little homology, but it has been reported that they share common features which may be required for the translocation process (Austen, 1979; Austen & Ridd, 1981; Austen et al., 1984). Although some exported prote ...
... (Blobel & Dobberstein, 1975). The primary sequences of signal sequence peptides exhibit little homology, but it has been reported that they share common features which may be required for the translocation process (Austen, 1979; Austen & Ridd, 1981; Austen et al., 1984). Although some exported prote ...
13-2 PowerPoint
... Ribosomes use the sequence of codons in mRNA to assemble amino acids into polypeptide chains. The decoding of an mRNA message into a protein is a process known as translation. ...
... Ribosomes use the sequence of codons in mRNA to assemble amino acids into polypeptide chains. The decoding of an mRNA message into a protein is a process known as translation. ...
No Slide Title
... • If the sequence of the protein of unknown structure has greater than 40% identity with one of known structure, the structure prediction problem may be considered solved – especially with the ...
... • If the sequence of the protein of unknown structure has greater than 40% identity with one of known structure, the structure prediction problem may be considered solved – especially with the ...
EXPLORING PROTEIN STRUCTURE
... down into single amino acids ready for use in building new proteins. And yes, proteins have the job of digesting proteins, they are known as proteases. There are only 20 different amino acids but they can be joined together in many different combinations to form the diverse range of proteins that ex ...
... down into single amino acids ready for use in building new proteins. And yes, proteins have the job of digesting proteins, they are known as proteases. There are only 20 different amino acids but they can be joined together in many different combinations to form the diverse range of proteins that ex ...
The Cell Membrane
... Active Transport Cells may need to move molecules against concentration gradient ...
... Active Transport Cells may need to move molecules against concentration gradient ...
Sp100 is important for the stimulatory effect of
... 1990). The Sp100 family of proteins comprises the Sp110, Sp140/LYSp100 and the autoimmune regulator protein (AIRE), a transactivator which is mutated in a hereditary autoimmune disease (Vogel et al., 2002). All members of the Sp100 protein family share an N-terminal HSR (homogeneously staining regio ...
... 1990). The Sp100 family of proteins comprises the Sp110, Sp140/LYSp100 and the autoimmune regulator protein (AIRE), a transactivator which is mutated in a hereditary autoimmune disease (Vogel et al., 2002). All members of the Sp100 protein family share an N-terminal HSR (homogeneously staining regio ...
没有幻灯片标题
... and temperature, each protein folds spontaneously into one three-dimensional conformation, called the native conformation. 3.1.2 This conformation is usually thermodynamically the most stable (having the lowest Gibb’s free energy), and predominates among the innumerable theoretically possible ones. ...
... and temperature, each protein folds spontaneously into one three-dimensional conformation, called the native conformation. 3.1.2 This conformation is usually thermodynamically the most stable (having the lowest Gibb’s free energy), and predominates among the innumerable theoretically possible ones. ...
cis667-12
... where, g1 and g2 are based on the given model M. g1 shows how each segment corresponds to core segment i in the model, and g2 deals with the interactions between segments. So to solve the threading problem, we have to decide on t1, t2, t3, …, tm, so that the overall score is maximum. Thus the thread ...
... where, g1 and g2 are based on the given model M. g1 shows how each segment corresponds to core segment i in the model, and g2 deals with the interactions between segments. So to solve the threading problem, we have to decide on t1, t2, t3, …, tm, so that the overall score is maximum. Thus the thread ...