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Ubiquitin and Ub
Ubiquitin and Ub

...  DRiPs represent polypeptides that never attain native structure owing to errors in translation or post-translational processes necessary for the proper biogenesis of the proteins  Schubert et al.* found that upwards of 30% of all newly-synthesized proteins from various cell types are degraded by ...
Caffeine as a cause of coral bleaching: Effects of caffeine on
Caffeine as a cause of coral bleaching: Effects of caffeine on

... PhastSystem with gradient 8-14 gels. The results indicated that our extraction procedure was successful in producing sufficient yields of protein; however, the resolution of the gels was too poor to show differences in the protein bands. We are now sending our extracted proteins to the School of Bio ...
chapter3_part1 Membrane lecture
chapter3_part1 Membrane lecture

... transport systems • Specificity -- each carrier protein is specialized to transport a specific substance ...
The antibody validation experiments were essentially performed as
The antibody validation experiments were essentially performed as

... 4. The siRNA transfection mix was stepwise diluted to a final concentration of 13 nM and subsequently transferred into the designated well of the 96-well plates (Greiner, SensoPlate). 5. The plates were dried in a speed-vac, GeneVac MiVac Quattro, initially for 90 min, then for 45 min, and thereafte ...
Kidney Transporters
Kidney Transporters

... active transport -H-ATPase secretes H+ at apical membrane via primary active transport ...
HA Tagged Yeast Clones and Collection - Technical
HA Tagged Yeast Clones and Collection - Technical

... through triple hemagglutinin epitope tag (3xHA tag), remains. The mTn technology has proven useful for large-scale functional analysis of the yeast genome. The HA tags permit a variety of functional studies including immunolocalization, immunoprecipitation and analysis of binding sites using immuno ...
Cytochrome P450 3A4: The Impossible Protein
Cytochrome P450 3A4: The Impossible Protein

... It is speculated that the specific P450, 3A4, can bind two drugs at the same time, and in ...
Complete genomic sequence of viral hemorrhagic septicemia virus
Complete genomic sequence of viral hemorrhagic septicemia virus

... Within the P gene of VSV, an additional overlapping reading frame was detected (14) encoding a deduced protein C, which is localized in cytoplasmic compartiments of virus infected cells. In the VHSV and IHNV genomes, an additional second ORF contained in the P gene is also present. The deduced hypot ...
Paper - Journal of Environmental Biology
Paper - Journal of Environmental Biology

... further analysis. In the present study, the primary structure of peptide were predicted by using the Expasy's ProtParam server (Gasteiger et al., 2005). The results showed that ALLCE-AMP had 120 amino acid residues and estimated molecular weight was 13737.3. The calculated isoelectric point would be ...
Protein Translation
Protein Translation

... Three codons serve as stop signs: UAA, UAG or UGA ...
Number 49, 2002 13 Balázs Csóka , Tamás Zeke
Number 49, 2002 13 Balázs Csóka , Tamás Zeke

... transformants by RT -PCR and W estern blotting, respectively. Ho wever, the transformation did no t result in detectable change s in phenotype. This work demonstrates that the alcA promoter, a conditional gene expression system widely used in both Aspergillus and higher plants, also functions in N. ...
Glycosylation of the capsid proteins of cowpea mosaic virus: a
Glycosylation of the capsid proteins of cowpea mosaic virus: a

... conclusions regarding glycosylation, at least as far as amino sugars were concerned. To confirm the apparent lack of glycosylation of CPMV capsids, a total sugar analysis was undertaken. It was necessary to perform this analysis on the isolated capsid proteins as the vigorous hydrolysis conditions r ...
462a Reading and Homework Assignment 3
462a Reading and Homework Assignment 3

... Protein 1 (Ramachandran plot A) is T4 lysozyme, which is almost all -helical. Protein 2 (Ramachandran plot B) is TATA binding protein, which has a lot of both helix and -sheet. *How to draw a helical wheel. A helical wheel is a two-dimensional representation of a helix obtained by projecting the ...
Endo-1-06-99_1-20-99
Endo-1-06-99_1-20-99

... The hypothalamus releases TRH, which acts on the pituitary, causing it to release TSH, which acts on the thyroid, causing it to release T3 and T4, which act on the target cell, the hypothalamus, and the pituitary. T3 and T4 feed back to the hypothalamus and the pituitary. High levels of T3 and T4 sh ...
structure-tertiary-text
structure-tertiary-text

... CHMI Cross-sectional 2227 - E.R. Gauthier, Ph.D. ...
Lipid modifications of proteins – slipping in and out of membranes
Lipid modifications of proteins – slipping in and out of membranes

... regulatory mechanism for shuttling proteins between the cytosol and the membranes or between the different membrane domains. Agonist-stimulated turnover of palmitate is found in a small number of proteins, including the b-adrenergic receptor and certain G-protein a-subunits2,3. However, the instabil ...
Membrane Bound: C2-Domain Abscisic Acid
Membrane Bound: C2-Domain Abscisic Acid

... cytosolic proteins can reside on the periphery of vesicles, and they can transiently interact with membranes for trafficking or signaling purposes; these associations are driven by protein modules that recognize specific features of proteins or membranes (Cho and Stahelin, 2005). The identities of t ...
50695_1 - Griffith Research Online
50695_1 - Griffith Research Online

... To extend this work into a HP approach we have altered the current algorithm and created a new algorithm. Instead of just looking at the length of the sub-conformation, we now introduce a ‘Hydropathy Index Measure’(HIM) which is two fold. First of all for each sub-conformation a sum of each residues ...
CPP1
CPP1

... the reduction of protochlorophyllide (Pchlide) to chlorophyllide, is catalyzed by the lightdependent protochlorophyllide oxidoreductase (POR). Here we report that chaperone-like protein of POR 1 (CPP1), an essential protein for chloroplast development, plays a role in the regulation of POR stability ...
mnw2yr_lec16_2004
mnw2yr_lec16_2004

... (George & Heringa, Prot. Struct. Func. Genet., in press; 2002) ...
PPT
PPT

... After the sequence or secondary structure alignment is complete, SuperPose then generates a difference distance (DD) matrix between aligned alpha carbon atoms. A difference distance matrix can be generated by first calculating the distances between all pairs of C atoms in one molecule to generate an ...
HUMAN NUTRITION
HUMAN NUTRITION

... Other nine cannot be made by the body and must come from the diet These nine are called essential amino acids ...
Organic Compounds
Organic Compounds

... amino acids ( Figure 1.2) that connect together like beads on a necklace ( Figure 1.3). There are only 20 common amino acids needed to build proteins. These amino acids form in thousands of different combinations, making about 100,000 or more unique proteins in humans. Proteins can differ in both th ...
Exam 1 - UCLA Chemistry and Biochemistry
Exam 1 - UCLA Chemistry and Biochemistry

... b. (4) How many different disaccharides may be formed from the condensation of these two sugars? (Assume that mutarotation may occur before and/or after the reaction.) Show ...
C2006/F2402 `14 Outline Of Lecture #2 -
C2006/F2402 `14 Outline Of Lecture #2 -

... Note: The text in Becker equates a tetramer with a protofilament; this does not appear to be the usual terminology. All other texts and the diagram in Becker diagram consider a protofilament to be a long structure formed by many tetramers. 3. Stability -- Some IF cables do disassemble/reassemble dur ...
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Magnesium transporter

This page links directly from the magnesium in biological systems page.Magnesium transporters are proteins that transport magnesium across the cell membrane. All forms of life require magnesium, yet the molecular mechanisms of Mg2+ uptake from the environment and the distribution of this vital element within the organism are only slowly being elucidated.In bacteria, Mg2+ is probably mainly supplied by the CorA protein and, where the CorA protein is absent, by the MgtE protein. In yeast the initial uptake is via the Alr1p and Alr2p proteins, but at this stage the only internal Mg2+ distributing protein identified is Mrs2p. Within the protozoa only one Mg2+ transporter (XntAp) has been identified. In metazoa, Mrs2p and MgtE homologues have been identified, along with two novel Mg2+ transport systems TRPM6/TRPM7 and PCLN-1. Finally, in plants, a family of Mrs2p homologues has been identified along with another novel protein, AtMHX.
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