Arabidopsis Formin3 Directs the Formation of Actin
... The actin cytoskeleton is a highly organized but complex and dynamic structure in eukaryotic cells. The actin cytoskeleton is formed by the assembly (or polymerization) of G-actin monomers into filamentous actin (F-actin or actin microfilaments) and is generally known to participate in cell growth, ...
... The actin cytoskeleton is a highly organized but complex and dynamic structure in eukaryotic cells. The actin cytoskeleton is formed by the assembly (or polymerization) of G-actin monomers into filamentous actin (F-actin or actin microfilaments) and is generally known to participate in cell growth, ...
Ectopic expression of beta-cytoplasmic actin
... Recently, Fyrberg and co-workers have used the IFM to test the consequences of isoform-specific amino acid replacements in Act88F actin (Reedy et al., 1991; Fyrberg et al., 1998). When a single isoform-specific amino acid of Act88F was substituted with a residue corresponding to another Drosophila a ...
... Recently, Fyrberg and co-workers have used the IFM to test the consequences of isoform-specific amino acid replacements in Act88F actin (Reedy et al., 1991; Fyrberg et al., 1998). When a single isoform-specific amino acid of Act88F was substituted with a residue corresponding to another Drosophila a ...
Mathematical Biology
... Assuming a one dimensional geometry is a simplification for mathematical tractability at this preliminary modelling stage. It is suitable for describing filopodia, but may not be as accurate for a lamellipod where the filaments have more variance in their orientation. Behaviour at the membrane. We a ...
... Assuming a one dimensional geometry is a simplification for mathematical tractability at this preliminary modelling stage. It is suitable for describing filopodia, but may not be as accurate for a lamellipod where the filaments have more variance in their orientation. Behaviour at the membrane. We a ...
The Plant Actin Cytoskeleton Responds to Signals from Microbe
... Plants are constantly exposed to a large and diverse array of microbes; however, most plants are immune to the majority of potential invaders and susceptible to only a small subset of pathogens. The cytoskeleton comprises a dynamic intracellular framework that responds rapidly to biotic stresses and ...
... Plants are constantly exposed to a large and diverse array of microbes; however, most plants are immune to the majority of potential invaders and susceptible to only a small subset of pathogens. The cytoskeleton comprises a dynamic intracellular framework that responds rapidly to biotic stresses and ...
Get PDF file - Botanik in Bonn
... diamine and 50% glycerol to minimize fading of the ¯uorescent conjugate. Images of immuno¯uorescently labeled samples were collected using a confocal microscope (TCS4D; Leica, Heidelberg, Germany). Several controls were performed to determine the speci®city of the antibody labelings, including incub ...
... diamine and 50% glycerol to minimize fading of the ¯uorescent conjugate. Images of immuno¯uorescently labeled samples were collected using a confocal microscope (TCS4D; Leica, Heidelberg, Germany). Several controls were performed to determine the speci®city of the antibody labelings, including incub ...
A single-headed fission yeast myosin V transports actin in a
... tail mediated by Rng8/9. A modified motility assay showed that this additional binding site anchors Myo51–Rng8/9 so that it can cross-link and slide actin-tropomyosin filaments relative to one another, functions that may explain the role of this motor in contractile ring assembly. ...
... tail mediated by Rng8/9. A modified motility assay showed that this additional binding site anchors Myo51–Rng8/9 so that it can cross-link and slide actin-tropomyosin filaments relative to one another, functions that may explain the role of this motor in contractile ring assembly. ...
Actin Dynamics in Muscle Cells
... generation of contraction. In non-muscle cells, actin filaments are very dynamic and regulated by an array of proteins that interact with actin filaments and/or monomeric actin. Interestingly, in non-muscle cells the barbed ends of the filaments are the predominant assembly place, whereas in muscle cel ...
... generation of contraction. In non-muscle cells, actin filaments are very dynamic and regulated by an array of proteins that interact with actin filaments and/or monomeric actin. Interestingly, in non-muscle cells the barbed ends of the filaments are the predominant assembly place, whereas in muscle cel ...
Multiple Roles of the Cytoskeleton in Bacterial Autophagy
... actin tails by relieving the autoinhibited state of N-WASP [31]. In summary, microtubules promote vesicle trafficking required for autophagy and can be targeted by bacterial pathogens for intracellular survival. Future work investigating the role of microtubule dynamics in the maintenance of pathoge ...
... actin tails by relieving the autoinhibited state of N-WASP [31]. In summary, microtubules promote vesicle trafficking required for autophagy and can be targeted by bacterial pathogens for intracellular survival. Future work investigating the role of microtubule dynamics in the maintenance of pathoge ...
The actin cytoskeleton is a target of the self
... plants has attempted to characterize the effect that signalling stimuli have on actin dynamics or, more speci®cally, the state of actin polymerization. Tip growing cells provide a good system whereby the signals responsible for mediating changes to the actin cytoskeleton may be studied. In plants, p ...
... plants has attempted to characterize the effect that signalling stimuli have on actin dynamics or, more speci®cally, the state of actin polymerization. Tip growing cells provide a good system whereby the signals responsible for mediating changes to the actin cytoskeleton may be studied. In plants, p ...
Coordination of microtubule and microfilament dynamics by
... vivo. The direct interaction between Rho1 and Capu suggests an additional level of complexity ...
... vivo. The direct interaction between Rho1 and Capu suggests an additional level of complexity ...
Capping protein: new insights into mechanism
... S100B should bind to whole wild-type CP in solution. No such interaction has been observed in several in vitro physical binding and functional assays carried out with high concentrations of protein under conditions in which S100B binds tightly to the 12- and 28-residue a-subunit peptides and to dena ...
... S100B should bind to whole wild-type CP in solution. No such interaction has been observed in several in vitro physical binding and functional assays carried out with high concentrations of protein under conditions in which S100B binds tightly to the 12- and 28-residue a-subunit peptides and to dena ...
Live cell imaging reveals actin-cytoskeleton
... Luxembourg. 2Plateforme d’Imagerie Cellulaire et Tissulaire PTIBC-IBISA, CNRSUMR 7563 et FR3209. Université de Lorraine, F-54505 Vandoeuvre-lès-Nancy, France. ...
... Luxembourg. 2Plateforme d’Imagerie Cellulaire et Tissulaire PTIBC-IBISA, CNRSUMR 7563 et FR3209. Université de Lorraine, F-54505 Vandoeuvre-lès-Nancy, France. ...
Hormones and Signal Transduction III
... RAS Swaps GDP for GTP on Activation RAS Slowly Cleaves GTP to GDP ...
... RAS Swaps GDP for GTP on Activation RAS Slowly Cleaves GTP to GDP ...
articles
... pathways has been documented both in vitro and in vivo2,4,5. However, it is unclear whether EMT is regulated as a single process or at the level of its individual cellular events. Cadherins, which can mediate differential adhesion, are commonly viewed as a central target6–8. The picture emerging fro ...
... pathways has been documented both in vitro and in vivo2,4,5. However, it is unclear whether EMT is regulated as a single process or at the level of its individual cellular events. Cadherins, which can mediate differential adhesion, are commonly viewed as a central target6–8. The picture emerging fro ...
From filaments to function: The role of the plant actin cytoskeleton in
... targeting of this network, the actin-endomembrane systems have been characterized through work demonstrating actindependent hijacking of the ER by the plant enveloped virus Tomato spotted wilt tospovirus (TSVW; (Feng et al. 2013; Ribeiro et al. 2013); Figure 2A; Table 1). In brief, the TSWV membrane ...
... targeting of this network, the actin-endomembrane systems have been characterized through work demonstrating actindependent hijacking of the ER by the plant enveloped virus Tomato spotted wilt tospovirus (TSVW; (Feng et al. 2013; Ribeiro et al. 2013); Figure 2A; Table 1). In brief, the TSWV membrane ...
The Pathogen-Actin Connection: A Platform for Defense Signaling in
... signaling, but also with basal plant processes such as development (95), transcription (58), and rearrangement of the actin cytoskeleton (129). Therefore, gene-for-gene resistance has morphed into what is now commonly referred to as the guard hypothesis (reviewed in 19). The guard hypothesis offers ...
... signaling, but also with basal plant processes such as development (95), transcription (58), and rearrangement of the actin cytoskeleton (129). Therefore, gene-for-gene resistance has morphed into what is now commonly referred to as the guard hypothesis (reviewed in 19). The guard hypothesis offers ...
Autoregulation of Actin Synthesis by Physiological
... emission wavelengths were 542 and 563 nm. Cellular protein was determined according to Bradford (21) in parallely cultured hepato^ cytes lysed in the presence of 5 g/1 Triton X-100. Data were expressed as ng rhodamine-phalloidin per mg cellular protein. In addition, the ratio of filamentous to non-f ...
... emission wavelengths were 542 and 563 nm. Cellular protein was determined according to Bradford (21) in parallely cultured hepato^ cytes lysed in the presence of 5 g/1 Triton X-100. Data were expressed as ng rhodamine-phalloidin per mg cellular protein. In addition, the ratio of filamentous to non-f ...
Characterization of the Enzymatic Component of the ADP
... that these mutants had no or largely reduced ADP-ribosyltransferase activity. To test the CDTa mutant proteins in more detail, a time course study of the ADP-ribosylation of actin from platelet cytosol (Fig. 5A) and of actin from rabbit skeletal muscle (Fig. 5B) was performed. Cytotoxic effects of r ...
... that these mutants had no or largely reduced ADP-ribosyltransferase activity. To test the CDTa mutant proteins in more detail, a time course study of the ADP-ribosylation of actin from platelet cytosol (Fig. 5A) and of actin from rabbit skeletal muscle (Fig. 5B) was performed. Cytotoxic effects of r ...
Control of the Actin Cytoskeleton in Plant Cell Growth
... monomer incorporation at the barbed end beyond the limits of free diffusion (80). Unlike the putative plant Arp2/3 complex, plant formins have been studied to some extent in vitro. Four plant formins (AtFH1, AtFH4, AtFH5, and AtFH8) have been shown to nucleate purified actin, and allow extension from ...
... monomer incorporation at the barbed end beyond the limits of free diffusion (80). Unlike the putative plant Arp2/3 complex, plant formins have been studied to some extent in vitro. Four plant formins (AtFH1, AtFH4, AtFH5, and AtFH8) have been shown to nucleate purified actin, and allow extension from ...
Rho signaling pathway and apical constriction in the - ICB-USP
... placode toward the underlying optic cup, the edges fuse and detach from the surface ectoderm, forming the lens vesicle (Schook, 1980b). A variety of mechanisms have been suggested to contribute to the initial inward buckling of the placode, such as differential cell proliferation or death, tissue in ...
... placode toward the underlying optic cup, the edges fuse and detach from the surface ectoderm, forming the lens vesicle (Schook, 1980b). A variety of mechanisms have been suggested to contribute to the initial inward buckling of the placode, such as differential cell proliferation or death, tissue in ...
Molecular mechanism of protrusion formation during Listeria Keith Ireton Luciano A. Rigano
... The structural analysis of Tuba SH3-6 with its physiological partner N-WASP, revealed a typical SH3 domain structure for Tuba SH3-6 consisting of a five-stranded, antiparallel β-barrel with a 310 -helix in loop β4–β5 (Polle et al., 2014). The prolinerich peptide 346 pppalpssapsg357 of N-WASP adopts ...
... The structural analysis of Tuba SH3-6 with its physiological partner N-WASP, revealed a typical SH3 domain structure for Tuba SH3-6 consisting of a five-stranded, antiparallel β-barrel with a 310 -helix in loop β4–β5 (Polle et al., 2014). The prolinerich peptide 346 pppalpssapsg357 of N-WASP adopts ...
Molecular Machinery Regulating Exocytosis
... nucleotide-dependent conformational transitions within their G-protein domain (PereiraLeal & Seabra, 2000), these GTPases act as molecular switches; cycling between the inactive GDP bound form and a GTP-bound active form, the process which regulates the activity of downstream effectors. This activit ...
... nucleotide-dependent conformational transitions within their G-protein domain (PereiraLeal & Seabra, 2000), these GTPases act as molecular switches; cycling between the inactive GDP bound form and a GTP-bound active form, the process which regulates the activity of downstream effectors. This activit ...
Identification of plant cytoskeleton-interacting proteins
... cDNA library from Arabidopsis was inserted into rat fibroblasts and screened for fluorescent chimeras localizing to cytoskeletal structures. The high-throughput screen was performed by an automated microscope. An initial set of candidate genes identified in the screen was isolated, sequenced, the fu ...
... cDNA library from Arabidopsis was inserted into rat fibroblasts and screened for fluorescent chimeras localizing to cytoskeletal structures. The high-throughput screen was performed by an automated microscope. An initial set of candidate genes identified in the screen was isolated, sequenced, the fu ...
Neuronal polarity: from extracellular signals to
... amount of plasma membrane (by vesicle recruitment and fusion); an increase in the local concentration and activation of signalling molecules (such as phosphatidylinositol 3-kinase (PI3K) and Rho GTPase) and their receptors; an increase in the dynamics of actin filaments; and the enhancement of micro ...
... amount of plasma membrane (by vesicle recruitment and fusion); an increase in the local concentration and activation of signalling molecules (such as phosphatidylinositol 3-kinase (PI3K) and Rho GTPase) and their receptors; an increase in the dynamics of actin filaments; and the enhancement of micro ...
Neuronal Growth Cone Retraction Relies Upon Proneurotrophin
... p75NTR and the closely related SorCS2 family member, sortilin, as compared to p75NTR alone. Among the proteins detected by mass spectrometry, we identified Trio, a scaffold protein containing Rac and Rho GEF domains (16) (Fig. 2A and fig. S3). We confirmed the interaction between p75NTR and Trio by ...
... p75NTR and the closely related SorCS2 family member, sortilin, as compared to p75NTR alone. Among the proteins detected by mass spectrometry, we identified Trio, a scaffold protein containing Rac and Rho GEF domains (16) (Fig. 2A and fig. S3). We confirmed the interaction between p75NTR and Trio by ...