Download 3. What are the intrinsic and extrinsic environments of exoplanets?

The Astrophysics of life:
Exoplanets and extremophiles
_______________________________
Principal Investigator
I. N. Reid
Space Telescope Science Institute*
3700 San Martin Drive
Baltimore, MD 21218
*operated by Association of Universities for Research in Astronomy, Inc. (AURA)
_________________________________
Co-Investigators
Space Telescope Science Institute
C. Christian
D.R. Soderblom
M. Livio
W.B. Sparks
A. Nota
J. Valenti
M. Postman
Princeton University
E.L. Turner
C.F. Chyba
N.J. Kasdin
M. Littman
Center of Marine Biotechnology, University of Maryland
S. DasSarma
F. Robb
F. Chen
K. Sowers
Carnegie Institution of Washington
M. Turnbull
B. Paczynski
D. Spergel
S. Tremaine
R. Vanderbei
_______________________________
Collaborators and Consultants
Space Telescope Science Institute
R. Gilliland
P. McCullough
S. Lubow
K. Sahu
University of Maryland
J. Coker
A. Frederick
A. Colman
Carnegie Institution of Washington
M. Fogel
A. Steele
Princeton University
T.C. Onstott
University of New South Wales
R. Cavicchioli
Morgan State University
J. Müller
NIST
T. Germer
Idaho State University
L. DeVeaux
National Astronomical Observatory of Japan
M. Tamura
T. Yamada
Smithsonian Astrophysical Observatory
J. Winn
University of Melbourne
S. Wyithe
Oxford Brookes University
S. McCready
Tokyo University
Y Suto
Jet Propulsion Laboratory
R. Carlson
Notice of Restriction on Use and Disclosure of Proposal Information
The information (data) contained in Volume 2, Section 6 (Budget) of this proposal constitutes a trade secret and/or information
that is commercial or financial and confidential or privileged. It is furnished to the Government in confidence with the
understanding that it will not, without permission of the offeror, be used or disclose other than for evaluation purposes; provided,
however, that in the event a contract (or other agreement) is awarded on the basis of this proposal, the Government shall have the
right to use and disclose this information to the extent provided in the contract (or other agreement). This restriction does not
limit the Government’s right to use or disclose this information (data) if obtained from another source without restriction.
Confidential
Page 0
6/20/2017
Executive Summary ........................................................................................................................ 2
Summary of Personnel, Commitments and Cost ............................................................................ 5
1. Research and Management Plan: Introduction ........................................................................... 6
2. Where are the exoplanets? .......................................................................................................... 9
3. What are the intrinsic and extrinsic environments of exoplanets? ........................................... 22
4. Can the exoplanets support life? ............................................................................................... 29
5. How will we recognize if life is present?.................................................................................. 47
6. Management Plan...................................................................................................................... 61
7. Strengthening the NAI Community .......................................................................................... 66
8. Education, Outreach and Press Presence .................................................................................. 73
9. References ................................................................................................................................. 83
10. Appendix: Glossary of terms and acronyms ........................................................................... 97
Confidential
Page 1
6/20/2017
Executive Summary
Synopsis: A team of leading scientists and engineers located at the Space Telescope Science
Institute (STScI, lead institution), the Center of Marine Biotechnology (COMB) at the University
of Maryland Biotechnology Institute and in the School of Engineering and Department of
Astrophysical Sciences at Princeton University (PU) will collaborate to form a new NAI node
focused on the scientific goal of identifying habitable exoplanets, the collaborative purpose of
expanding the NAI’s connections to and between the exoplanet and extremophile research
communities and a suite of outreach/education/training projects targeted on diverse audiences,
including the general public, K-12 level students and teachers, PU undergraduates and the
members of relevant scientific communities. The ultimate purpose of the team’s efforts is to
expand our understanding of the potential for life in environments beyond, and probably even
more extreme than, those present on Earth and in the Solar System.
Unifying Intellectual Themes and Focus: Research on extremophiles, particularly
psychrophiles, and on exoplanetary systems are the two major scientific themes of the proposed
research program and are supplemented by some investigations of Solar System objects that
seem particularly likely to provide useful lessons concerning the astrobiological significance of
extremophiles and for future astrobiological investigations of exoplanets. Although a diverse set
of astronomical and biological research projects are proposed, all are connected to the goal of
identifying habitable planets via their relevance to one of the four major questions around which
the overall effort is organized:
1 - Where are the exoplanets?
2 - What are their intrinsic and extrinsic environments?
3 - Can they support life?
4 - How will we recognize if life is present?
These four questions are addressed by projects described in sections 2, 3, 4 and 5 (respectively)
of the Research and Management Plan and each is the topic of major research efforts centered in
either two or all three of the participating institutions, and through a collaboration with scientists
at other NAI nodes and beyond. Thus the proposed research, in turn, directly addresses three
Astrobiology Roadmap goals:
#1 – “Understand the nature and distribution of habitable environments in the Universe”,
#5 – “Understand the evolutionary mechanisms and environmental limits of life”, and
#7 – “Determine how to recognize signatures of life on other worlds and on early Earth”.
It also indirectly addresses important aspects of a fourth goal:
#2 – “Explore for past or present habitable environments, pre-biotic chemistry and signs of life
elsewhere in the Solar System”.
These issues are central to “Earth-like Planets and Life”, one of the four key research areas in
NASA’s Vision for Space Exploration.
Research Program: The research programs associated with each of the four questions listed
above are summarized below:
1. Where are the exoplanets? We will compile a Solar Neighborhood Habitability Census
(SNHC), and use this investigative tool to determine the potential for habitable worlds around
other stars. This compendium will go beyond the conventional paradigm, paying particular
attention to stars with lower mass than the Sun, notably M dwarfs (contributing to the SETI
Institute NAI Team’s M-star initiative), and binary systems. We will provide comprehensive,
Confidential
Page 2
6/20/2017
carefully vetted information on both the stars themselves and their immediate environments. The
compilation will focus on the nearest stars, but will include all known exoplanetary systems,
including new results from ground and space based observations (e.g., microlensing surveys,
high precision spectroscopy of transiting “hot Jupiter” systems, Kepler GO observing programs).
We will use the SNHC to clarify what is known about the Sun’s neighbors as potential hosts of
habitable exoplanets. We will identify stars that lack critical observations and, where possible,
acquire the appropriate data through supplementary observations. In particular, the PU
coronagraphic optics group is developing high-contrast imaging techniques that will be
invaluable in identifying faint companions of nearby stars from the ground and in studying
terrestrial exoplanets directly via future space missions. We will combine the observed stellar
properties with constraints on life-limiting conditions, including those derived from experiments
on extremophiles proposed here, to estimate the likelihood that individual exoplanet systems are
capable of harboring habitable exoplanets.
2. What are their intrinsic and extrinsic environments? We know that planetary environments
depend on the properties and behavior of the central star, the planet’s orbital characteristics and
the exoplanet atmospheric and surface properties. Terrestrial exoplanets are likely to remain
below the detection threshold for direct imaging for at least a decade, although they should be
detected in both radial velocity and transit surveys (the Kepler mission); gas giants, however, are
potentially accessible to direct imaging, while their atmospheric properties are already being
measured for transiting systems. Such observations are invaluable in determining whether
exoplanets, including “hot Jupiters” (gas giants on ~3-day orbits around solar-type stars), closely
resemble gas giants in our Solar System. We will pursue further observations of planetary
exospheres, capitalising on results from transit surveys and high-contrast coronagraphy. Specific
observing programs will utilize the Hubble Space Telescope, very high resolution and signal-tonoise spectroscopy with the Subaru 8.2-meter telescope and large telescope spectroscopic and
photometric surveys. We will also investigate the dynamical evolution of exoplanetary systems,
particularly examining the distribution of orbital eccentricity and long-term dynamical stability.
3. Can they support life? Not all exoplanets, and perhaps very few, will be able to support life.
We will match our assessment of the likely conditions on exoplanets against terrestrial life’s
experimentally determined limits; this requires a reliable set of metrics for the limiting
environments which allow life’s survival and growth. Determining the low temperature limit for
life is particularly important, since cold environments are likely to be far more common than
warm ones. This holds even for Earth, where 80% of the biosphere by volume exists at average
temperatures below 5o C. We will build on a heritage of over 2 years of experiments by the
COMB and STScI teams designed to examine cold limits for life, and investigate how life adapts
physiologically (e.g. through biofilm formation) and genetically (e.g., by induction of molecular
chaperones) to various types of extreme environments (see Reid et al, 2006). We will also
investigate the effects of cyclic variations in conditions, since many known exoplanets are on
elliptical orbits. Finally, we will examine the radiation sensitivity of extremophile species, and
consider the implications for survival in the presence of strong stellar activity, both on planetary
surfaces and in space (panspermia). The results from these experiments, along with other studies
of extremophiles within the NAI and elsewhere, will provide direct feedback to the SNHC.
These results will also be relevant to evaluating the prospects for life on, and planetary protection
measures appropriate for, Mars and Europa.
Confidential
Page 3
6/20/2017
4. How will we recognize if life is present? We will develop innovative methods for the
biocharacterization of exoplanets, observable only as faint point sources, a problem crucial to the
astrobiological significance of the very rapidly developing astronomical study of exoplanetary
systems. Specifically, we propose to conduct a series of observational experiments that will use
earthshine to characterize Earth as an effectively unresolved planet. This work will complement
and extend studies being carried out by existing NAI nodes (principally those centered at JPL
and the University of Arizona). In addition, we will investigate new methods of remote life
sensing, notably those based on polarization and high resolution spectroscopic biosignatures. We
will test these techniques through observations of Mars, Europa and Enceladus.
Education/Public Outreach and Strengthening Astrobiology: In these areas, the team’s efforts
will build on existing, highly-successful activities at each of the three participating institutions:
STScI has a well established and highly acclaimed suite of programs for providing access to
basic information and recent results in astrophysics to both the media and K-12 educators; we
will add a major astrobiological component to these activities and use their well established
visibility to promote public and student appreciation of the field. PU will expand its current
course offerrings in astrobiology and seek to establish a “certificate program” in astrobiology
which Princeton undergraduates will be able to attach to their diplomas by satisfying an
appropriate set of academic requirements, and PU CoIs will work closely with the existing
undergraduate-initiated “astrobiology club” to promote campus interest in the field. A group of
Princeton faculty, including all PU CoIs on this proposal, will work to establish an
interdisciplinary Center for Planets and Life internal research unit. COMB will evolve its
existing annual Extremophile Summer School for graduate students, postdoctoral fellows and
other researchers into a five day “Extremophiles in Astrobiology: Theory and Techniques” semiannual summer offerring. This program, in which CoIs from all three institutions will
participate, is intended to be symmetrical with (and thus complementary to) the existing NAIsponsored summer programs at Arizona and Hawaii which are intended to familiarize lifescience and Earth-science students with planetary-science and astrophysics.
Management Plan: The management structure for the proposed NAI node will be relatively
simple but with the necessary formality required to assure productivity given the large, diverse
and dispersed nature of the project. In addition to the PI, with overall responsibility, there will be
institutional-PIs at COMB and PU who will coordinate local activities and be the primary point
of management and administrative contact with the PI and each other. Weekly CoI meetings at
each institution and bi-weekly full team video conferencing will provide good communication
and encourage close interaction of the various research projects as they progress. Appropriate
elements of the overall project (e.g., SNHC, coronograph development, psychrophile
characterization) will adhere to a schedule of milestones aimed at producing specific
deliverables. A 0.3 FTE Program Manager at STScI, with extensive experience in organizing
and steering large and complex technical projects, will assist the PI in matters of budget,
schedule and reporting, both within the team and for the team in the larger NAI environment.
Finally, the team will be advised by a small, external committee which will convene annually to
provide an independent review of all of the team’s activities and initiatives.
Confidential
Page 4
6/20/2017
Summary of Personnel, Commitments and Cost
Commitment
(total FTEs)
Name
Space Telescope Science Institute
I. N. Reid
Project manager
C. Christian
M. Livio
A. Nota
M. Postman
D. Soderblom
W. Sparks
J. Valenti
M. Turnbull
Postdoctoral fellow
Student intern
Sr. database engineer
Systems administrator
Web developer (E/PO)
Project manager
Animator (E/PO)
Informal science educator (E/PO)
E/PO evaluator
2.50
1.50
0.50
0.50
0.50
0.50
0.50
0.50
0.25
5.00
5.00
1.92
0.18
0.10
0.48
0.50
0.12
0.32
0.21
Center of Marine Biotechnology
S. DasSarma
J. Coker
F. Chen
Graduate student
F. Robb
A. Colman
K. Sowers
Graduate student
1.25
5.00
0.50
5.00
0.50
5.00
0.50
5.00
Princeton University
E. Turner
C. Chyba
K. Hand
J. Kasdin
M. Littman
B. Paczynski
D. Spergel
S. Tremaine
R. Vanderbei
Graduate students (2.3) Astrophysics/TBN
Graduate student Engineering/TBN
M. Carr (Sr. Technical Staff)
Confidential
Page 5
1.25
0.50
1.00
0.50
0.50
0.50
0.50
0.50
0.50
11.5
5.00
1.65
6/20/2017
1. Research and Management Plan: Introduction
The central theme of our proposal is identifying habitable exoplanets. This is a central issue for
astrobiology, which seeks to address fundamental questions concerning our place in the Universe
(see Chyba and Hand, 2005). In this proposal, we take the conventional definition of habitability,
and the conventional habitable zone (Kasting, Whitmire & Reynolds, 1993) as a starting point
for our investigations, but we explore means of expanding those limits.We also focus primarily
on simple microbial species, primarily archaea, and frame our discussions of habitable (and
inhabited) worlds largely in that context. The individual research components of this proposal
tackle four key questions whose answers work in concert to address this larger purpose: 1)
Where are the exoplanets? 2) What are their intrinsic and extrinsic environments? 3) Can they
support life? 4) How can we tell if they do?
We propose a suite of research projects tied to these four questions. We address the integration
of the individual science elements in Section 1.2; here we note that an overarching goal of this
proposal, as with the NAI as a whole, is to bring together these different sciences (and scientists)
to form a common vocabulary.
1.1 Our team
Astrobiology is by its nature interdisciplinary, and a correspondingly interdisciplinary team of
scientists has come together to tackle our research program. We include experts on extremophile
biology from the Center of Marine Biotechnology (COMB); builders of coronagraphic optics and
imaging analysis from Princeton University and Space Telescope Science Institute (STScI);
specialists on planetary “demographics,” the constituents of the Solar Neighborhood, and
database construction and maintenance from STScI, the Carnegie Institution of Washington
(CIW) and Princeton; researchers of planetary dynamics from Princeton and STScI; and experts
on Earthshine and planetary biosignatures from Princeton, STScI and CIW. We will be
collaborating with researchers from ten other institutions, including Idaho State University,
Morgan State University, the University of Tokyo, the University of Melbourne, NIST, JPL,
SAO, the National Astronomical Observatory of Japan, the University of New South Wales and
Oxford Brookes University. Our team also includes experienced individuals in education,
outreach and the public understanding of science, and our E/PO program will be co-ordinated
from STScI. In addition, Princeton University is developing a Center for Planets and Life, and
offers astrobiology courses at both the undergraduate and graduate level. Graduate students, and,
where appropriate, undergraduates from Princeton Unversity, the University of Maryland,
Morgan State University, and Johns Hopkins University will be directly involved in the research
outlined in this proposal.
1.2 A project synthesis
We have proposed a diverse set of projects covering a broad range of biological and
astronomical topics. How do these individual projects interact with each other? Figure 1.1
illustrates the cross-fertilization: the first version of the Solar Neighborhood Census (Section 2.2,
2.3) will be created from basic stellar data; initial habitability criteria will be based on the
characteristics of the known exoplanets, suggesting targets for further observation; as more
exoplanets are identified (Section 2.4), we will refine the SNH Census and the dossier of
Confidential
Page 6
6/20/2017
exoplanet characteristics (Section 3); the results from our biological experiments on how life
adapts to cold and is affected by radiation (Section 4) will likely improve our understanding of
the environments of exoplanets and Solar System bodies, and modify our habitability criteria
(and the SNH Census, and hence target selection for ground-based observations and space
missions); at the same time, analyses and observations of Solar System bodies (Sections 4.4 and
5.5) are likely to influence our biological experiments, and will certainly inform our search for
biosignatures (Section 5.2, 5.3 and 5.4) that have potential for future space missions like TPF,
Darwin and Lifefinder.
Exoplanet detection
Solar Neighborhood
Habitability Census
Characteristics of
exoplanets
Stellar data
Mars, Europa and
Enceladus
Cold and Life
Radiation and Life
New biosignatures
Figure 1.1 The main modules of our proposed research program: individual modules are color-coded by the
underlying question: green – question 1 (Section 2); blue – question 2 (Section 3); yellow – question 3 (Section
4); red – question 4 (Section 5); our proposed research on Mars, Europa and Enceladus is relevant to both
questions 3 and 4.
We will foster interactions between team members through regular meetings of our full team of
CoIs and collaborators. As described in Section 7.6, we plan to offer Extremophile Summer
Schools at COMB in years 1, 3 and 5; we will take advantage of those opportunities to hold 1-2
day symposia, where we will present and discuss results from the individual projects. During
years 2 and 4, we will hold 3 to 4-day offsite retreats, including representatives from the wider
NAI community.
1.3 Research products
We propose to produce the following in this program:
Confidential
Page 7
6/20/2017





First, a major product of our research will be the Solar Neighborhood Habitability
Census, an active database that will collect and synthesize key data for stars and their
immediate environments. We will use this Census ourselves as an investigative tool for
our astrobiological research, but this compilation will also prove invaluable in focusing
both ground-based observing campaigns and future NASA missions, including Kepler,
the James Webb Space Telescope (JWST), SIM Planetquest, the Terrestrial Planet Finder
(TPF) and Lifefinder. A key property of the Census is that it will go beyond merely
compiling all available astronomical data for the objects of concern; instead, we will use
the acquired knowledge, expertise, and judgment of the team to critically assess and
evaluate the available observations, both astronomical and biological, and create a
research tool of broad use to the entire astrobiology community. Finally, the Census will
be capable of drawing upon the entire research community through adaptability and the
inclusion of feedback from others, as well as continuous growth in what the Census
includes and how it makes that information available.
A second result from this program will be the development and implementation of novel,
innovative methods of high-contrast coronagraphic imaging. We will use the associated
instrumentation to survey nearby stars for close, faint companions.
Third, we will provide new information on how life adapts to low temperature, high
radiation and other extreme environments. This work will have clear implications on the
prospects for life on Mars and Europa. It will also directly bear on preventing the
potential forward contamination of these bodies by microbes that could be present on
spacecraft from Earth.
Fourth, we will use Solar System objects as reference cases for characterization tools that
will be applied to exoplanets.
Finally, we will examine Earth as a planet, and identify and characterize new
biosignatures suitable for remote life detection. In particular, we will examine the
potential of circular polarization as an unambiguous biosignature, sensitive to life at the
broadest level, from simple microbial species to complex terrestrial scenes.
In addition to these research goals, we are committed to educational and public outreach.
 Graduate and undergraduate students will be directly involved in our research,
thus helping to train the coming generation of astrobiologists.
 We will share our research experience and results with students and the public at
large through innovative educational and informational resources.
Our team members bring skills to the NAI that complement the current range of scientific
disciplines: in particular, we have
 Wide-ranging expertise on archaeal extremophiles, particularly cold-adapted species and
the biogeochremistry of extreme environments;
 A profound understanding of the properties of stars in the Solar Neighborhood;
 In-depth knowledge of cutting-edge high-contrast imaging technology and techniques.
Each of these research areas is crucial to successfully identifying habitable, Earth-like planets,
and achieving one of the prime goals of NASA’s Vision for exploration.
Confidential
Page 8
6/20/2017
2. Where are the exoplanets?
Goal 1 of the 2002 Astrobiology Roadmap is straightforward: Determine the potential for
habitable planets beyond the Solar System, and characterize those that are observable. It is
conceivable, of course, that life could exist in the interstellar medium or other, more exotic,
locales, but given what we know so far, this Goal boils down to understanding the basic
properties of the stars of our Galaxy, how those properties translate into potential habitable zones
in the immediate environments of those stars, and how stable those environments remain over
the long time scales we associate with biological evolution.
We propose to meet this goal through a thorough and comprehensive effort to characterize
nearby stars and to derive the astrobiologically important parameters of those stars. To do this
we will construct the Solar Neighborhood Habitability Census, a comprehensive database
encapsulating key information on the properties of a complete, well-defined stellar sample. As
the name suggests, this database focuses on stars near the Sun, but we will also incorporate data
for all stars known to have planetary-mass companions. We will not simply amass all available
measurements for a given star; rather, we will use the expertise of our team to assess the quality
of individual observations and derived parameters, and determine the most reliable
measurements for each star in the database. Our main purpose in constructing the SNH Census is
to provide an investigative software tool for our own astrobiological research, and to build a
bridge from an astrophysical compilation to a facility that our biologist colleagues can draw on
to inform their work. The Census will also reach beyond the team of this proposal to the entire
astrobiology and NAI community.
As we construct the database, we will identify stars that lack crucial data, and, wherever
possible, we will obtain the new observations needed to supply the missing information (see
Section 2.3.2). Direct detections of planetary companions are of obvious importance, and our
team includes key players in several major exoplanet surveys that use a variety of methods,
including radial velocities, direct imaging, and photometric monitoring for either transits or
microlensing events. In particular, the Princeton Coronagraphic Optics group is developing new
high-contrast imaging techniques as a major part of this proposal, and those techniques will be
applied to search for low luminosity companions (potentially even gas giant exoplanets) around
nearby stars. We will incorporate results from those programs and other planet detection
surveys, including those associated with the UCLA and CIW NAI nodes, as they become
available.
Once in place, the SNH Census will allow us to investigate habitability in a number of ways. For
example,
 Systematic properties of the exoplanet host stars can provide clues to the required
conditions for planet formation. The most striking example is the correlation between
exoplanet frequency and metallicity (Gonzalez, 1997; Fischer & Valenti, 2005).
 We can investigate whether the known host stars might harbor habitable exoplanets.
Dynamical considerations may rule out the presence of exoplanets with stable orbits in
the (conventional) habitable zone (see Section 3.3), while observations of planetary
exospheres can provide important information on chemical composition (Section 3.2).
Confidential
Page 9
6/20/2017

Conversely, we can eliminate field stars that are ill-suited to be habitable exoplanet hosts
because of their intrinsic characteristics such as variability, close stellar or brown dwarf
companions, or excessive emission of high energy radiation.
Applying habitability criteria requires a working definition of life’s limits for survival and
growth, an issue tackled by several current NAI programs; some aspects are adressed in
Section 4 of this proposal.
2.1 Why a new census?
Why undertake this SNH Census at all? Aren’t there already databases for the nearby stars?
And what is gained by compiling existing information over new research? Is there value added?
Extensive work already exists in nearby-star studies, and a number of compilations of those data
are either underway or have been completed recently. These include the NStars database
(http://nstars.arc.nasa.gov/index.cfm), the Catalog of Habitable Stellar Systems (HabCat:
Turnbull & Tarter, 2003a, b), the TPF target list (http://sco.stsci.edu/tpf_tibdb) and the StARS
database. However, none of these is well suited to the needs of the research in this proposal or to
the needs of astrobiology in general.
 First, there is a mismatch in scope. For instance, the NStars database aimed to include all
stars within 25 parsecs of the Sun, but the database is no longer maintained, and partial
data are available for only a limited subset of stars. The current planet search programs
include stars to 40-50 pc, so at best NStars would provide information on a small subset
of the stars that have known planetary companions. By comparison, the HabCat database
(co-authored by CoI Turnbull) is derived primarily from the Hipparcos and Tycho-2
catalogues, and therefore is dominated by more luminous stars (especially G dwarfs, as
explained below), and includes few of the many nearby M dwarfs. The TPF target list
focuses specifically on the needs of that NASA mission, and consists mainly of solar-type
stars within only 15 parsecs of the Sun. Finally, the full scope of the StARS database,
which is still under construction, remains unclear; however, this database is designed to
serve a much wider community and a broader range of purposes, while our research is
focused specifically on habitability issues.
 Second, most existing databases amass all the available data for their objects. Our
approach is that not all data are created equal, and that a carefully vetted and reviewed
census is of much greater value than an uncritical assemblage of everything that may be
in print. Our team includes astronomers with career-long familiarity with the different
kinds of stars we will include and the observations available for those stars. By applying
the knowledge, expertise and judgment of our team we believe we can build a database of
higher consistent quality than otherwise possible. Moreover, we do not see ourselves as
necessarily the last word, and the SNH Census will provide a means for anyone in the
astrobiology community to provide their own assessments.
 Third, these databases tend to stop at the point that astronomers feel comfortable, which
is dealing strictly with astronomical data. A Census intended to serve the astrobiology
community should go beyond that to interpret the astrophysical data in terms that are
useful to exobiologists, and to extend the database in ways that help make it a tool for the
biologists to inform their own work. This is a critical point at which our collaboration
between astronomers and biologists really matters and lies at the heart of the concept of
an NAI.
Confidential
Page 10
6/20/2017


Fourth, current databases tend to be conceived in terms of a particular point in time, even
if the data itself get updated. In contrast, we are still in the process of identifying subsolar mass M dwarfs within 20 parsecs, and we expect to add more stars to the SNH
Census over the duration of this NAI project. We also will update and even expand the
database as new or revised relevant measurements become available. This goal is
achieved most efficiently with an in-project database.
Finally, STScI has well-known strengths in the construction and execution of databases
and data archives, notably the observations obtained by HST, but also other missions and
other kinds of data products. STScI has the capability to do the SNH Census well.
Our overall purpose is to create the SNHC as an investigative tool for our research, not as yet
another reference dataset for the general community. Consequently, the database must be
responsive to our needs.
2.2 Sampling the Solar Neighborhood
The first step in building the SNH Census is the definition of the sample to be included, and the
means to identify the members of those families. We will focus on the stars nearest the Sun as
our primary sample, with other stars of obvious interest (those with known exoplanets) added for
their context. Do these nearest stars adequately represent the Galaxy as a whole?
2.2.1 The Solar neighborhood as a Galactic microcosm
Stars near the Sun have long been favorite targets for astronomical research for two main
reasons. First, the nearest stars include the brightest members (in apparent magnitude) of any
spectral class. These nearby stars provide us with the highest possible photon fluxes for our
telescopes, and can therefore be studied in the greatest detail. Also, linear spatial resolution
decreases in proportion to the distance of the observed object, so nearby stars offer the best
prospects for identifying close (and faint) orbital companions – a key issue for exoplanet
surveys. We look at the nearby stars simply because we have the chance to see more.
Concentrating on the nearest stars is also important if we desire a well-defined sample with
statistically reliable properties. Stellar catalogs become increasingly incomplete with increasing
distance, and the effective distance limit for completeness is less for intrinsically fainter stars.
Those fainter stars are simply harder to enumerate, of course, among the vast numbers of distant
objects in our Galaxy. Distance measurements themselves become less accurate with increasing
distance. It is important to bear these limitations in mind when analyzing the average properties
(and the distribution of properties) of stellar systems because incomplete samples almost always
incorporate hidden or poorly understood biases that can skew the results.
Demanding a complete sample restricts our survey to relatively tiny volume of the Milky Way.
For example, for sun-like stars, catalogs become incomplete at distances of ~40 parsecs (less
than 0.2% the Galactic radius). Despite this, the solar neighborhood in fact includes stars that
were born in many different parts of our Galaxy and over the Galaxy’s full history. This is
because stars interact gravitationally with each other and with more massive objects, notably
Giant Molecular Clouds. Those interactions tend to scatter stellar velocities, so that stars that
may have formed together end up well dispersed and well mixed over astronomical time scales.
Confidential
Page 11
6/20/2017
Not every kind of star is represented in the Solar neighborhood. For example, halo stars (low
metallicity members of the Galaxy’s first major stellar population) are extremely rare simply
because their number density is low. Similarly, there are few extremely young stars (less than 10
Myrs in age) locally, since the star lies relatively far from active star forming regions. On the
other hand, the nearest examples of these systems are under active study by other members of the
NAI, including UCLA astronomers (e.g., Zuckerman & Song, 2004) and members of the
University of Arizona NAI team. Setting aside these minor exceptions, the local stars are
representative of the broad bulk of stars in the Galactic disk, and are therefore likely to provide a
fair estimate of the prevalence of habitable environments within the Galaxy as a whole.
2.2.2 Which stars to include?
In putting together a representative cross-section of the Galaxy’s stars, it is not sufficient to
simply specify a distance limit. Our ideal sample would be complete and volume-limited; i.e., it
would include all stars within some specified horizon. In practice, this ideal is neither practical
nor feasible. When stars form, the distribution of their masses follows a power law, so that there
are huge numbers at the lowest masses, and many fewer at high masses. At the same time,
massive stars are inherently much more luminous that stars of lower mass and so can be seen at
great distances. Low-mass stars get fainter much faster than they get numerous, so that the M
dwarfs comprise fully 80% of our Galaxy by number, yet constitute a mere handful of the
100,000 brightest stars.
What this means is that a well-constructed census of the nearby stars will balance these factors to
come up with definitions that are best suited to different kinds of stars. This is summarized in
Table 2.1:
Spectral
types1
B, A, F and G
K
M to L
Absolute magnitude
MV < +6
+6 < MV < +8
MV > +8
Distance
(parsecs)
40
25
20
limit
Number
systems
2,000
350
2,000
of
These choices for distance limits are driven by practical considerations. The first family, of B,
A, F, and G stars, is now straightforward to determine because of the precise astrometric
observations carried out by the satellite Hipparcos of the European Space Agency. Hipparcos
observed essentially all stars brighter than 9th magnitude in V, roughly 75,000 stars. Most of
those stars are intrinsically luminous, and too far away for our purposes. Of the remainder, a
significant portion is made up of G dwarfs, i.e., stars that are very similar to the Sun. This is
because stars more massive than the Sun are inherently less numerous, and, also to some extent
because such stars evolve more quickly than the Sun does, so that few are left on the main
sequence. Thus the observations made by Hipparcos make it straightforward to list the G dwarfs
(and hotter, earlier types) that are within 40 pc. This has already been done by CoI Soderblom as
part of his effort to measure stellar activity in solar-type stars. Many of these stars are in planet
search programs (Nidever et al., 2002), and most now have high-quality radial velocities and
metallicities.
1
The spectral type system is an astronomical shorthand that provides a basic characterization of individual stars. For
historical reasons, the spectral type sequence is OBAFGKMLT (where L and T were added very recently), and runs
from hot, high-mass stars to cool, sub-stellar mass brown dwarfs. The Sun is type G2.
Confidential
Page 12
6/20/2017
On the other hand, the Hipparcos sample included only a modest number of K dwarfs because
only the very nearest were bright enough. However, analyses indicate that this sample of
Hipparcos K dwarfs is effectively complete within 25 parsecs (Reid, Gizis & Hawley, 2002).
Finally, nearby M and L dwarfs are the targets of an on-going campaign, the ML-NStars survey
(Reid & Cruz, 2001; Cruz et al, 2003), based on near-infrared data from the 2-Micron All-Sky
Survey (2MASS). So far, results are available for approximately half the sky (Reid et al., 2004),
with follow-up observations underway for the remainder.
The combination of these three families results in a total of ~4,350 stellar systems, sufficiently
small numbers to allow detailed consideration of each source, but sufficiently large to provide
reliable statistics.
About 30% of the stars with known planetary-mass companions lie beyond these distance limits.
This is only a small number of objects, and so we will supplement the Solar Neighborhood
sample with data for all of those stars, regardless of distance. While selection effects may bias
planet detection in these more distant stars, those effects should be apparent from comparison
with the solar neighborhood sample; indeed, trends and biases among the exoplanet hosts are of
intrinsic interest, potentially shedding light on the exoplanet formation process, and it is
important to have the full context available.
2.3 Creating the Solar Neighborhood Habitability Census
“..there are known knowns; there are things we know we know. We also know that there are
known unknowns; that is to say we know there are some things we do not know. But there are
also unknown unknowns – the ones we don’t know we don’t know.”
(D. Rumsfeld, February 2002)
2.3.1 Building the database
The SNH Census is designed to incorporate astrometric, photometric, spectroscopic, and
multiplicity data for the nearest stars. In brief, we need to understand what we know about the
stars in the Solar Neighborhood, how well we know it, and what we don’t know: we aim to
maximize known knowns, identify known unknowns, and minimize the Rumsfeldian unknown
unknowns.
The information collected in the database will be divided conceptually into several categories.
Identifiers, such as the Hipparcos or HD catalogue number are needed to enable crosscorrelations with other databases. Phase space data will describe the position and motion of the
star with respect to the solar system barycenter. Right ascension, declination, parallax, proper
motions, and heliocentric radial velocity are all examples of phase space data. Phase space data
are useful for identifying kinematic groups. Classifications will describe morphological
characteristics, such as spectral class and multiplicity. Photometry will compile apparent
magnitudes in standard filters and quantities derived from those data. Examples include
Johnson/Cousins UBVRI optical broadband magnitudes, EB-V color excess, 2MASS near-infrared
JHK magnitudes, and mid-infrared excess flux above the predicted photospheric level. Physical
properties will describe fundamental stellar properties, usually determined by detailed analysis.
Confidential
Page 13
6/20/2017
Effective temperature, gravity, rotation, and overall metallicities or individual elemental
abundances are examples. Empirical properties will describe stellar measurements of observable
characteristics that lack a fundamental interpretation. Observations of stellar activity fall into this
category, since activity cannot be quantitatively linked to surface magnetic field properties.
Derived quantities will be a function of other database columns. The function may involve
interpolation in a table of model results (e.g. bolometric corrections or ages based on isochrone
fitting). The derived quantities will also include parameters of relevance and use to our biologist
colleagues, such as the flux of UV and X-ray radiation, and the HZ location.
We will pay particular attention to assembling data on known companions and the circumstellar
environments of the nearby stars. In particular, we will record constraints (including upper limits
on potential companions) from precision radial velocity surveys, astrometric monitoring, direct
imaging, and transit monitoring. We will record measurements and limits on extended emission
at infrared and radio wavelengths. These factors are crucial to assessing the likelihood of finding
a habitable planet around an individual star, yet no existing (or proposed) database comes close
to providing all this information. We will not only compile and catalog such data, but our
proposed database will highlight well-defined areas of doubt and uncertainty in our knowledge
of planetary systems in the solar neighborhood. We will specifically target stars lacking crucial
data in follow-up observing programs with ground-based and space-based observatories.
Finally, we will couple the astronomical data with biological criteria to define habitability
indices for each star. CoI Turnbull already has considerable experience in this area from her
work on both HabCat (Turnbull & Tarter, 2003a) and the TPF target database. We anticipate
including indices based on such factors as the presence and orbital characteristics of any
companions, the circumstellar environment, the level of stellar activity, the metallicity of the
parent star and the location of the habitable zone. We expect that both individual indices and the
criteria themselves will evolve over the course of this project.
Data collection and archive contents: Basic data, such as photometry, astrometry or
metallicity, will be garnered from the literature or our own observations. In many cases, there are
multiple measurements of standard parameters, such as (B-V) color. Under those circumstances
we will use our considered judgment to select the most reliable value (or values). Where
necessary, we will apply appropriate adjustments to different sets of measurements to give selfconsistent results; for example, using different chemical abundance indicators can lead to
systematic offsets in the zeropoint and/or scale of stellar metallicities (Reid, 2002).
Besides individual parameters, we will include relevant images and spectra in the SNHC
database. In addition to our own optical and infrared spectra, we will incorporate archived data
from ground-based and space missions, either directly or through links to the appropriate
archives. We will also provide appropriate tools for combining multiwavelength datasets.
Implementing the database: We will construct the SNH Census as a relational database,
allowing high functionality and easy access. STScI uses this type of database, specifically SQL
databases, to organize data associated with HST, JWST and particularly the Multimission
Archive at STScI (MAST). We will achieve an efficient implementation of the SNH Census by
adapting tools used to create and maintain existing STScI databases. We will ensure that the
Confidential
Page 14
6/20/2017
protocols are consistent with National Virtual Observatory (NVO) requirements, to ensure the
widest access to our compendium of results.
We plan the following schedule:
 During Year 1 we will compile the initial version of the database, populated with basic
stellar data.
 We will implement the first set of habitability crtieria, modeled on Habcat, in Year 2, and
release version 1.0 of the SNH Census to our NAI team members, and solicit their input;
identify key missing data and, where necessary, initiate related observing projects.
 In Year 3 we will update and expand the Census; incorporate initial results from
dynamical simulations (Section 3.3) in habitability criteria. We will release v2.0 of the
SNHC to the NAI general community, and solicit their input.
 In Year 4 we expect to be able to include initial results from the Kepler mission,
providing data on statistical properties of gas giant exoplanets, if not terrestrial planets.
We will continue to acquire key missing data.
 In Year 5 we will release v3.0 of the SNH Census, with updated habitability criteria and a
more complete census of the nearest stars.
2.3.2 Supplementary observing programs
A crucial aspect of our proposed research is our intention to create the SNH Census as an active
database, rather than simply capturing the sum of knowledge at one moment in time. We will
identify significant defects in our knowledge of the nearest stars, and actively pursue their
remedies. Even at this juncture, there are obvious gaps in our knowledge that we are addressing
through either on-going observing programs, or programs that will be initiated in the near future.
Close binary companions
Close binary systems are generally unfavorable as hosts for habitable exoplanets, since stable
planetary orbits generally lie well beyond the conventional habitable zone. Observationally,
detecting close, low mass companions is challenging, and many nearby stars, particularly M
dwarfs, have not yet been subjected to sufficient scrutiny to determine their true multiplicity. We
envisage several observational programs to tackle this issue.
Subaru high-contrast coronagraphic near-infrared survey of nearby stars: HiCIAO (High
Contrast Coronagraphic Imager with Adaptive Optics) is a second-generation instrument
currently under construction by the exoplanet research group at NAOJ (National Astronomical
Observatory of Japan), led by Dr. M. Tamura, for use on the Subaru 8.2-meter telescope at
Mauna Kea. HiCIAO will be used in conjunction with Subaru’s new 188 actuator AO system
and laser guide star facility and with planned future “extreme AO” systems. HiCIAO will have
multiple optical modules including the AO module, the warm coronagraph pupil plane module,
the high contrast optics module and cold IR camera module. It will be a uniquely powerful high
contrast imaging system that is expected to improve over the performance of all existing such
systems by at least a factor of 10 and to be the first such system to combine polarization and
spectral differential imaging modes. In addition, its modular designed allows ongoing
improvements and development of new coronagraphic techniques, including experimentation
with pupil plane mask designs. HiCIAO first light is currently planned for December 2006, and
Confidential
Page 15
6/20/2017
an extensive campaign of coronagraphic surveys will commence in 2007 and are expected to
conclude in roughly 2011.
We (CoIs Kasdin, Littman, Spergel, Turner and Vanderbei) will collaborate with Dr. Tamura and
his group in both the experimental development of high contrast coronagraphy using HiCIAO
and on extensive survey observations of nearby stars deemed to be of interest for exoplanet
studies. In particular, the Princeton coronagraph group will design and construct pupil plane
masks for use in an appropriate HiCIAO front-end module, and NIR high contrast images will be
obtained of suitable targets. The latter observations will aim to discover and characterize faint
companions and/or low surface brightness circumstellar material. Additional high resolution
spectroscopic observations (using the APO Echelle, for example) and thermal infrared
measurements using Spitzer and/or ASTRO-F will also be obtained. The results will be
integrated into the Solar Neighborhood Habatibility Census.
AO imaging of young nearby M dwarfs: Brown dwarfs and giant exoplanets fade and cool
rapidly with time; young stellar systems therefore offer the best prospects for direct imaging of
these very low-mass companions. M dwarfs have lower luminosities than solar-type stars, and
therefore better contrast ratios for detection. Young M dwarfs have particularly active
chromospheres and coronae, particularly from X-ray observations. As part of the ML-NStars
survey, approximately 100 Pleiades-age M dwarfs have been identified, and those stars are being
imaged using both conventional AO (on the Gemini North telescope) and the Spitzer midinfrared space telescope (programs led by PI Reid). The Gemini observations have the potential
to detect 15 to 10 Jupiter-mass companions at Jovian separations (5-10 AU, where 1 AU is the
Earth-Sun distance). Non-detections are equally significant, since they set upper limits on the
frequency of such massive companions, and hence on their potential to de-stabilize lower-mass
planetary systems. These systems will also be targeted in the future using HiCIAO and new highcontrast coronagraphic techniques (see Section 2.4.1).
Exoplanets, stellar metallicities and ages
Doppler planets are found with higher frequency around stars with super-solar metallicities
(Fischer & Valenti, 2005). Detailed analyses of the high-resolution spectra used to search for
planetary companions also provide important information on properties such as individual
elemental abundances and stellar ages (Valenti & Fischer, 2005). Several hosts have abundance
ratios consistent with membership of the Galactic thick disk, indicating that planet formation has
occurred over the full lifetime of the disk (Reid, 2006). CoI Valenti is continuing analyses of
stars observed in on-going radial-velocity planet surveys.
2.4 Exoplanet detection programs
Even with over 130 planetary systems currently known, statistics are fairly sparse. Thus,
identifying additional extrasolar planetary systems is a high priority. This section outlines planet
detection programs that are being undertaken by members of the current NAI team. These
include searches for transiting or resolved systems, which offer the potential for direct
observations of the planetary companions, and therefore the opportunity to compare exoplanets
with the Solar System. We are also pursuing microlensing surveys, which probe lower masses
and different regions of the Galaxy.
Confidential
Page 16
6/20/2017
2.4.1 High-contrast coronagraphy and planet detection
Direct imaging of exoplanets is an essential objective for both astrobiology and NASA’s
Exploration Program. This includes the challenging task of detecting and characterizing Earth
analogs via the Terrestrial Planet Finder missions (TPF-C and TPF-I) as well as detecting gas
giants or low-mass brown dwarfs in Earth-like orbits about nearby stars. This latter task is less
challenging (a Jovian analogue at 1 AU from a sunlike star has a contrast ratio of 10 -8, as
compared to 10-10 for an Earth-analogue), but yet has profound implications for system
habitability. NAI funding will both 1) allow us to continue our technology research into
coronagraphic instrumentation techniques suitable for the extremely high-contrast needed for
terrestrial planet detection from space and 2) allow us to expand our activities into ground based
applications that will advance the state-of-the art for detection of low-mass companions and the
resolution of the structure of dust and debris disks orbiting nearby stars.
Princeton University is a leader in pupil plane coronagraphic technologies, and CoIs Kasdin,
Littman, Vanderbei and Spergel have supported NASA research into high-contrast imaging for
TPF since 2001. They have a fully operational high-contrast imaging laboratory where tests of
coronagraphs and wavefront control systems are ongoing. The technology being developed at
Princeton is critical for the support of future space observatories. In addition, we are also
collaborating on programs and instrumentation for ground based 8-meter class telescopes,
including mask support for the extreme AO work on Gemini (led by Lawrence Livermore
National Laboratory) and in coronagraph technology for the Subaru infra-red survey.
Figure 2.1: (Left) An image of the residual speckle in the dark region of the elliptical mask PSF after a 1
second exposure. (Middle) A cross-section of the experimental and theoretical PSF; black is the theoretical
PSF, blue is the experiment, and red is the Airy function envelope. (Right) The experimental image overlaid
with a simulation of the bright regions in the theoretical PSF that are blocked by a mask.
Figure 2.1 shows an example of our current results. Using an elliptical mask, we modify the
point-spread function (PSF) of an unresolved source (i.e. a star) to the shape mapped out by the
bright regions in the right-hand figure. We can used a “bow-tie” mask to block that light, giving
the left-hand image, which allows one to reach flux ratios exceeding 10 -5 at angular separations
beyond 4/D (where D is the diameter of the telescope mirror). The data plotted are for
monochromatic light, but we are achieving similar sensitivities with white light. This flux ratio is
comparable to that of hot jupiters or T-type brown dwarf companions to solar-type stars.
NAI-related activities
NAI support provides an ideal opportunity for an in-depth study of the engineering of remote
sensing and how it may enable new frontiers in extrasolar astrobiology and is a major part of our
Confidential
Page 17
6/20/2017
overall research program. We will continue to pursue both theoretical and experimental paths to
understanding and mitigating the issues associated with high-contrast imaging from both space
and the ground. We will apply our pupil amplitude profiling technology to as broad a spectrum
of high-contrast imaging applications as possible in support of the search for life. Our effort
will include continuing research into ultra-high contrast via pupil masks and wavefront control
for TPF, moving the technology to a high technical readiness level in preparation for the future
phase A opportunities. NAI funds will also support a student and faculty to examine optimized
pupil masks and data processing for ground based telescopes, such as Subaru, advancing the
technology to the point that we can propose construction of advanced instrumentation (using
additional non-NAI funds) and use them to carry out observing programs of the circumstellar
regions of nearby-stars.
In support of future space coronagraph missions, such as JWST and TPF, we will continue our
laboratory work in achieving ultra-high contrast via a combination of pupil masking and
wavefront control. Recently ordered deformable mirrors are expected to arrive soon, and the
first two years of NAI support for laboratory optical work in Princeton will be devoted to
demonstrating the viability of control algorithms, as well as testing pupil mask designs.
NAI support will is also allow us to initiate a technology research program intended to
demonstrate the viability of pupil mask coronagraphs for ground based, high contrast imaging.
This involves four major tasks: 1) design of shaped pupils compatible with telescopes containing
central obstructions and spiders (this is eased by the lower contrast requirements of the ground
observing program.), 2) Optimization of shaped pupils for spectroscopic and photometric
characterization of already discovered companions, 3) Complete performance analysis of ground
based planet imaging including full diffraction effects, atmospheric distortions, adaptive optics,
and speckle reducing software, and 4) High speed, image plane based adaptive optics. All
techniques and approaches will be tested and verified in our coronagraph laboratory at Princeton.
Our proposed research in coronagraphic techniques has direct synergy with the construction of
the Solar Neighborhood Habitability Census: some targets for the ground-based (and, eventually,
space) coronagraphic instruments will be identified from analyses based on the SNH Census;
conversely, the results of our observations will provide direct feedback to the Census, allowing
refinement of habitability criteria for individual stars and/or classes of stars.
2.4.2 Transiting systems
Planetary systems that transit the parent star are particularly important to astrobiology since these
systems provide the only currently available method of studying exoplanet dimensions and
probing the the atmospheric structure and composition. Following the initial discovery of HD
209458 (Charbonneau et al, 2002), a handful of other systems have been identified from
photometric monitoring programs (e.g. TrES-1, HD 149026 and several systems from OGLE).
All are hot Jupiters, with periods of less than 4 days. These systems provide vital information on
the intrinsic properties of exoplanets, allowing direct comparison with the well studied gas giants
in the Solar System. Both the XO project and the N2K survey are designed to add further
systems to the rather short list available at the present time.
Confidential
Page 18
6/20/2017
Collaborator P. McCullough is leading the XO project (McCullough et al, 2005), an active
observational program to discover additional hot-Jupiters that transit stars bright enough for
detailed studies in the immediate future with existing telescopes such as HST, Spitzer, Keck, and
ESO’s VLT, and later for future telescopes, such as JWST and SIM Planetquest. CoI Valenti is
closely involved in this program. Finding these systems in a timely manner is important in order
to make follow up observations with orbiting observatories; for example, Spitzer's expected life
lasts until late 2008. XO employs a dedicated telescope on Haleakala, Maui, to monitor
approximately 100,000 stars in a year, and is expected to discover two transiting hot Jupiters per
year for the next three years.
The N2K program (Fischer et al, 2005) is specifically designed to identify transiting hot Jupiters.
These observations target 14,000 FGK main-sequence stars and subgiants with V<10.5 and
distances within 110 parsecs of the Sun. CoI Valenti is also participating in this program,
particularly in detailed analysis of the high resolution spectra obtained in the course of the radial
velocity monitoring.
The last few years have seen advances in radial velocity precision that, when coupled with
intensive observations, have allowed detection of planets to 14 Earth Masses (Santos et al.,
2004). Within the next decade, surveys are expected to probe the existence of planets down to a
few Earth-masses for periods less than 10 days, and are likely to discover terrestrial-mass
transiting planets. JWST may well offer the potential to provide atmospheric diagnostics for
those planets.
Finally, the Kepler mission is scheduled for launch in June 2008, and is designed to search for
transiting terrestrial planet systems through high-accuracy (better than 2 x10-5) white light
photometry of 105 solar-type stars within a 100 square degree low-galactic latitude field in
Cygnus. Collaborator Gilliland is a CoI on the Kepler science team. Initial results from Kepler
will become available in the course of our NAI program, and those data, which will include
detections of gas giants, will provide invaluable data on planetary demographics. Observations
of all these systems will provide valuable input data on planetary properties for the SNH Census.
2.4.3 OGLE, Microlensing and planets
Microlensing surveys broaden the scope of planet detection to lower masses and different
Galactic environments. OGLE (Optical Gravitational Lensing Experiment) is a long term
collaborative project between Princeton and Warsaw University Observatories, using a 1.3 meter
telescope and a 8K x 8K pixel camera located at the Las Campanas Observatory in Chile to carry
out photometric monitoring campaigns of large numbers of stars in selected fields. OGLE has
already discovered several Jupiter mass planets, using two distinctly different methods:
gravitational microlensing and transits.
OGLE has discovered five confirmed transiting exoplanets (cf. Schneider 2005). OGLE data
have also led to the discovery of two Jovian planets by means of gravitational microlensing
(Udalski et al. 2004; Bond et al. 2005), and a new paper reporting the discovery of a third such
planet is ready for submission to Nature. This newly discovered exoplanet is much less massive
than Jupiter and is located at several AU from the primary star, within its habitable zone. We
Confidential
Page 19
6/20/2017
emphasize that gravitational microlensing is the only technique currently available that can
discover Earth mass planets orbiting in the habitable zone.
Over the next several years OGLE will streamline its software in two ways. Photometric
accuracy will be improved to bring it closer to the photon noise limit (Kruszewski and Semeniuk
2003, Tamuz et al. 2005). This will make it possible to detect shallower transits, and therefore
smaller planets. The OGLE automated alert system will be streamlined to be sensitive to smaller
anomalies in microlensing light curves (Udalski 2003). This will make it possible to detect
lower mass planets by means of microlensing, and it will significantly increase the detectability
of an Earth mass planet in the habitable zone.
As part of this NAI core team, CoI Paczynski, whose initiated Galactic microlensing studies
(Paczynski 1986) and who pioneered the idea of exoplanet detection via microlensing (Mao &
Paczynski 1991), will work with Princeton graduate students in the continuation and
improvement of the OGLE survey, with the goals both of discovering terrestrial mass exoplanets
in stellar habitable zones and of ultimately understanding their population statistics. These results
will provide direct feedback to the SNH Census on the potential frequency and semi-major axis
distributions of terrestrial-mass planets.
2.5 Using the SNHC: enabled research programs
Our purpose in constructing the SNH Census is to codify what we know about the stars in the
Solar neighborhood, with specific regard to their potential for supporting habitable exoplanets.
We will use that knowledge to focus follow-on research programs. Some of those programs will
emerge in the course of compiling the SNH Census; others can be identified in advance. Here,
we give three examples:
Exoplanet statistics
This is the most direct and immediate application of the SNH Census. Since our compilation will
include upper limits on exoplanet detections from radial velocity, imaging and other surveys, we
will identify the region of parameter space (mass, separation, apparent brightness) covered by
existing observations on a star-by-star basis. We will use statistical techniques, such as Bayesian
analysis (see Allen et al, 2005), to analyze the ensemble data and constrain the likely underlying
properties of planetary systems. These analyses will be particularly valuable, since we will be
working with a volume-complete sample.
Selecting nearby stars for high-contrast coronagraphy:
This program is directly synergistic between two key sections of our proposal, the SNH Census
and developing advanced coronagraphic instrumentation. Simple geometry dictates that the
nearest stars offer the best prospects for detecting resolved low-luminosity companions. We (PI
Reid, CoIs Kasdin, Turnbull, Turner, Valenti and Vanderbei and Soderblom) will use the SNH
Census to identify stellar systems offering the best potential for supporting habitable exoplanets,
and will target those systems for observation with HiCIAO and other ground-based
coronagraphs. The young, nearby M dwarfs that are already being scrutinized through
conventional AO observations are clearly high priority targets. Initially, these observations are
unlikely to be capable of directly detecting terrestrial exoplanets, but they have the potential to
Confidential
Page 20
6/20/2017
detect gas giants (or brown dwarfs). The orbital characteristics of those companions set strong
dynamical constraints on the potential existence of habitable terrestrial worlds (see Section 3.3).
Optimizing GO target selection for Kepler:
Kepler is effectively a pointed mission rather than a survey, since only data for pre-selected
sources within the 100-square degree area are saved. The targets will be chosen from the Kepler
Input Catalogue, which will include optical (Sloan) and near-infrared (2MASS) photometry for 5
million stars and will be released publicly. Besides the main program, Kepler will provide the
opportunity for Guest Observer programs targeting up to 3,025 stars at any given time, with the
observations having typical durations from 3 months to a year. Thus, Kepler has the potential to
observe ~50,000 additional sources over the 4 to 6 year lifetime of the mission.
We will use the results from our analysis of the SNH Census to guide selection of field K and M
dwarfs for a Kepler GO program to search for habitable exoplanet companions.The photometric
data from the Kepler Input Catalogue will permit an initial selection of candidates.
Chromospheric and coronal radiation have the potential to set significant constraints on the
prevalence of habitable M dwarf exoplanets. This is an issue that we will explore through both
astronomical observations (Section 3.4) and laboratory experiments (Section 4.3). We will use
follow-up spectroscopy of photometrically-selected candidates to identify sources with activity
levels most consistent with the requirements for habitability.
2.6 A census with a future
A "census" may suggest a one-time enumeration, but our Solar Neighborhood Habitability
Census will not only be maintained and updated, but will also grow and develop. Maintenance
will keep the database functional, and updating keeps the information in the database current, but
it is the augmentations to the Census that will add to its value over the course of our proposed
program and beyond.
As we noted above, the Census will be more than an astronomer's tool and will be adapted to
guide and inform the work of biologists too. That, in turn, will influence the astrophysical data
compiled, and, especially, the derived quantities that are relevant to assessing stars as sites for
habitable exoplanets. A vigorous and cogent collaboration of the kind the NAI supports will lead
to a database that is powerful and which has multiple uses.
2.7 Summary
The principal tasks outlined in this section are:
 Develop the Solar Neighborhood Habitability Census as an investigative tool for the
collection and analysis of astronomical and biological data relevant to the existence of
habitable exoplanets in nearby stars.
 Initiate and support surveys for exoplanets; in particular, develop advanced techniques
for high-contrast coronagraphic imaging with ground-based telescopes and in future
space missions.
Confidential
Page 21
6/20/2017
3. What are the intrinsic and extrinsic environments of
exoplanets?
3.1 Introduction
Characterizing planetary properties is a key part of the Goal #1 of the Astrobiology Roadmap.
Planetary environments and habitability depend on those properties, notably the atmosphere, and
on the external conditions imposed by the properties of the central star and the planet’s orbit. The
latter factors are relatively straightforward to estimate for individual exoplanetary systems. For
example, Figure 3.1 (from Reid et al, 2006) shows the ambient surface temperatures experienced
by an exact Earth analogue if it were a satellite of a known exoplanets. Many of those planets are
on elliptical orbits, leading to the substantial temperature variations illustrated in Figure 3.1;
those variations alone make it likely that only a handful of such hypothetical systems could
support life. Similarly, massive gas giants have consequences for the existence of as-yet
undetected terrestrial planets in stable orbits within the conventional habitable zone, while stellar
activity can hamper the development of life on ill-placed exoplanet companions. These issues are
explored in Sections 3.3 and 3.4.
Figure 3.1 Surface temperatures experienced by an Earth-analogue terrestrial satellite of the known gas-giant
exoplanets; solid red points identify systems where the primary star has evolved beyond the main sequence,
and the shaded region marks the approximate temperature limits for known terrestrial life.
Planetary atmospheres affect habitability. Present-day models are growing in sophistication, but
still lack strong observational constraints. One of our research goals is to obtain and interpret
new observations of planetary atmospheres and exospheres. At present, there are only limited
options for direct observations of exoplanet, notably spectral signatures during transits. Such
systems also allow measurement of planetary mass and radius, constraining structural models.
Confidential
Page 22
6/20/2017
3.2 Planetary atmospheres
Existing planet searches have established that in the Solar Neighborhood approximately 1 star in
200 has a “hot Jupiter” companion. Simple geometrical arguments imply that only ~1 in 2000
has a transiting planet (Fischer et al, 2005). Spectroscopic monitoring of ~4000 stars has yielded
only two transiting planets (HD 209458b and HD 149026b), and extensive photometric searches
for transits (mostly among much fainter stars) have so far revealed only another half dozen (see
section 2.4.2). Statistically, we anticipate that another dozen planets that transit bright (V<10)
stars await discovery. Members of our team will attempt to characterize the atmospheres of these
planets using a variety of techniques as they are discovered.
3.2.1 Space-based observations of exoplanet atmospheres
Planets that transit bright stars provide an important astrophysical laboratory for studying
planetary atmospheres. Empirical studies of exoplanet atmospheres can utilize two transitrelated techniques: (1) searches for absorption of starlight by the annulus of planetary
atmosphere as the planet transits in front of the star (e.g. Na in HD 209458, Charbonneau et al,
2002) and (2) searches for diminution of reflected starlight or planetary emission as the planet
passes behind the star (Charbonneau et al, 2005). In both cases, differential measurements
compare flux in and out of eclipse, which typically lasts a couple of hours. Only bright stars with
transiting planets can be measured precisely enough to study the planetary radius, atmospheric
composition (Charbonneau et al., 2002, Vidal-Madjar et al., 2004), exosphere extent (VidalMadjar et al., 2003), and infrared emission (Charbonneau et al., 2005; Deming et al., 2005).
Significant amounts of atomic hydrogen may fill the Roche lobe around a “hot Jupiter”, forming
an exosphere that covers 10-20% of the stellar surface during transit. This exosphere absorbs
stellar Ly- flux during transit, providing a measure of evaporation, which can be significant on
cosmological timescales. Only HST can currently measure this key far-UV diagnostic. Initially,
the Space Telescope Imaging Spectrograph (STIS) was used to study absorption of starlight by
hydrogen, carbon, oxygen, and sodium in the atmosphere and exosphere of HD 209458. During
multiple transits of HD 209458b, Vidal-Madjar et al. (2003) detected a 154% reduction in
stellar Ly flux, which they attributed to absorption by the planetary exosphere. The absorption
was stronger in the blue wing of Ly, suggesting outward acceleration of evaporated material
due to stellar radiation pressure.
While UV observations with STIS are no longer possible, similar data can be obtained using
prisms on the Advanced Camera for Surveys (ACS). We have an accepted HST program (led by
CoI Valenti) to directly detect the atmosphere and extended envelope (exosphere) of the recently
discovered transiting planet HD 149026b. These studies are not directly funded by this proposal,
but the results constrain planet formation scenarios, and feed directly into the SNH Census. .
3.2.2 Ground-based observations of transiting systems
Observations from the ground are also possible. As part of this proposal, we (CoI Turner,
working with Princeton graduate students and collaborators Y. Suto, J. Winn & T. Yamada) will
exploit the high dispersion and extremely high signal-to-noise spectroscopy obtained with large
ground based telescopes to characterize exoplanet systems via a variety of techniques. Extensive
data have already been obtained with the Subaru 8.2-meter telescope’s High Dispersion
Confidential
Page 23
6/20/2017
Spectrograph and with Apache Point Observatory’s 3.5-meter echelle spectrograph for multiple
“hot Jupiter” systems, particularly HD 209458b; further observations with these facilities are
planned and observing time using the Keck 10-meter telescopes’ HIRES spectrograph has been
requested. Integrations of tens of hours with an 8-meter class telescope on stars brighter than
10th magnitude at resolutions of nearly 105 yield signal-to-noise ratios of 103-104 per spectral
resolution element with elaborate and careful data reduction procedures (Winn et al. 2004), and
thus allow detection of small perturbations of the dominant stellar light due to the presence of the
exoplanet.
Such detectable perturbations include the exoplanet analogy (Queloz et al. 2000, Ohta, Taruya &
Suto 2005) of the Rossiter (1924) - McLaughlin (1924) binary star effect by which the
misalignment between the star’s spin angular momentum vector and the planet’s orbital angular
momentum can be measured (from line profile variations) for transiting systems. A
determination of this misalignment angle for HD209458b with unprecedented precision, 4.5 +/1.5 degrees, has recently been achieved by our group (Winn et al. 2005), and we plan to attempt
similar measurements for sufficiently bright transiting “hot Jupiter” systems, starting with TrES1 and HD189733. The distribution of this angle is a fundamental constraint on models of planet
formation and “migration” as well as containing information about the perturbation history of the
system subsequent to planet formation. Furthermore, if it could be established that this angle is
always, or nearly always, small (as it is in the two known cases of the Solar System and
HD209458b), it would provide a powerful aid to exoplanet detection and characterization studies
(see the review by Hale 1994 for details) and to targeting strategies for future exoplanet-related
space missions such as SIM and TPF.
The same data set can be used to search for the signatures of the evaporating atmosphere, the
exosphere, of a “hot Jupiter” exoplanet via transmission spectroscopy in the manner of VidalMadjar et al.’s (2003) detection of Lyman-alpha absorption associated with HD209458b transits.
Our group has already employed the data to place the strongest upper limits available to date on
exosphere absorption in several important optical lines due to the exosphere of the same system
(Winn et al. 2004, Narita et al. 2005).
Finally, these data can be used to search for starlight scattered from a “hot Jupiter’s” atmosphere.
This process will add a small component, proportional in strength to the planet’s albedo and
surface area, to the total detected light of the system containing a Doppler shifted (typically be
several 10s of km/s) copy of the stellar spectrum plus any spectral absorption features associated
with the planet’s atmosphere (Leigh, Cameron & Guillot 2003). A detection of, or strong upper
limits on, the strength of such a component of the system’s total light, would provide a powerful
probe of the atmospheric properties of this novel class of planets and be a major contribution to
their physical characterization. Half a dozen exoplanets well suited to studies of this type are
currently known and more will be discovered in the near future. Our team will also analyze the
data obtained in this project in an attempt to make the first direct detection of such scattered
exoplanet light.
3.3 Planetary dynamics
System habitability can be affected by dynamical interactions within planetary systems. Aspects
of these issues are being addressed by CoI Tremaine and collaborator Lubow. Their work
Confidential
Page 24
6/20/2017
complements ongoing research by current NAI teams at CIW, the University of Washington and
the Unversity of Arizona.
3.3.1 Dynamical evolution of planetary systems
Most work on planet formation is focused on the first Myr or so after star formation, during
which time planets are believed to form through a complex process involving four main stages:
(1) dust settles out of the cooling protoplanetary gas disk; (2) the dust concentrated in the midplane of the disk coalesces into km-sized bodies (planetesimals) through sticking processes or
gravitational instabilities; (3) the planetesimals grow by collisions into planetary embryos; (4)
the growth of the embryos stalls once they exhaust the material in their feeding zones, until the
cumulative effects of long-range gravitational interactions pump up their eccentricities to the
point where collisions and further growth into planets can occur (e.g., Lissauer 1993). We may
call this stage of planet formation “Phase I”.
The evolution of planetary systems during Phase I is difficult to disentangle, since the processes
are complex and observations are scarce. Collaborator Lubow is investigating the possible role of
disk/planet torques might play in defining the radial number distribution and eccentricity
distribution of exoplanet systems. Previous theoretical studies of migration have focused on the
properties of the so-called Lindblad resonances, where disk material sweeps through the
gravitational force field of the planet (Tanaka, Takeuchi, and Ward 2002 and references therein).
Corotational resonances, which have received less attention, are a potentially stronger influence
on early orbital evolution. Lubow, in collaboration with D'Angelo, Bate (Exeter) and Ogilvie
(Cambridge), has been analyzing the role of corotational resonances in disk/planet interactions.
They find that fast exoplanet migration occurs under certain conditions, and that material trapped
by the planet as it migrates can accelerate the migration. These interactions can also cause the
excitation of planetary eccentricity if the planet opens a gap in the disk material. The results from
these studies provide constraints on the potential existence of as-yet undetected low-mass
exoplanets in known planetary systems.
Following Phase I there is a second, much longer interval (5-10 Gyr; we call this “Phase II”) in
which the planets interact only through gravitational forces. During this stage planets can be
ejected into interstellar space, collide with the central star or other planets, be captured into
resonances via three-body interactions, etc. The importance of Phase II in determining the
properties of observed planetary systems is unknown, but a possible indication of its significance
is the observation that the outer solar system is “full”, in the sense that no other planet on a stable
orbit could be squeezed in between Jupiter and Neptune (Holman & Wisdom 1993); either this is
a coincidence or, more likely, there were once additional planets in the outer solar system that
were ejected during Phase II.
We (CoI Tremaine, working with Princeton graduate student M. Juric) have begun using
numerical models to investigate Phase II. The numerical integrations will be based on the
“Mercury” code (Chambers 1999), with extensive modifications to handle highly eccentric orbits
accurately and efficiently. As part of this NAI proposal, we will run the integrations on the
Astrophysical Sciences cluster computer (currently about 200 nodes with 3 GHz clock speed). A
major challenge to these studies is that the initial conditions are unknown; consequently, we need
Confidential
Page 25
6/20/2017
to consider a wide range of starting points. We expect to be able to run a suite of simulations,
typically 1000 planetary systems followed for 100 Myr, in about a week of wall-clock time.
Preliminary experiments suggest that: (1) most systems lose at least half their planets in Phase II;
(2) the surviving planets acquire a distribution of eccentricities similar to that seen in the sample
of known extrasolar planets; (3) the mean eccentricity in a system is anti-correlated with the
number of planets. The last of these results, in particular, suggests that planetary systems can be
arranged along a sequence: at one end are the commonest systems, with 1 – 3 planets having
relatively large eccentricities, while at the other are rarer systems having more planets with
smaller eccentricities. The observed extrasolar planetary systems are at one end of this sequence,
and the solar system at the other.
Not only will these simple experiments explore an important but neglected component of the
puzzle of planet formation, but if – as we expect – the Phase II evolutionary effects are strong,
then at least some properties of observed planetary systems may be deduced without a complete
understanding of the far more difficult Phase I.
3.3.2 Small planets
Since planets are believed to be formed by the accretion of vast numbers of small planetesimals,
and since this process stalls when the growing planets clear out their feeding zones, at masses
≈1024 g (Rafikov 2001), it is natural to wonder whether there are planetary systems in which this
process did not proceed as far as in our own system. Could there be systems containing hundreds
of lunar-mass bodies, or thousands of Ceres-mass bodies? Could these perhaps be far more
common than “terrestrial” and “giant” planets?
As part of this proposal, CoI Tremaine, working with Princeton graduate students, will
investigate the structure and dynamics of systems containing many small planets (in which the
overall mass of non-volatile elements in the planetary system is similar to what is observed in the
Solar System and extrasolar planetary systems, i.e., 10 – 100 Earth masses). In particular, we
shall ask:
 Under what circumstances can such systems survive to the present time, against
gravitational scattering and physical collisions?
 Under what circumstances could the growth of planetesimals into planets stall?
 How could we detect small-planet systems? The most promising possibility is to use
gravitational microlensing, because the surface density in the disk determines the overall
cross-section for microlensing by a planetary system, while the planet mass affects only
the duration of individual microlensing events.
 What is the relation of small-planet systems to debris disks (Rieke et al. 2005)?
3.4 M dwarf exoplanet environments
Solar-type stars (G, or sometimes F-K, dwarfs) have been the main focus of exoplanet
investigations over the last decade or more. These stars, however, are a minority constituent of
the Galactic disk: 80% of local stars are M dwarfs, with masses below 0.5 M⊙. In these systems
the conventional habitable zone lies at smaller separations; indeed, Kasting et al (1993) estimated
that planets in circular orbits in the habitable zone (HZ) of solar-age M dwarfs are likely to be
Confidential
Page 26
6/20/2017
tidally locked, with one hemisphere illuminated constantly by the central star and the other
perpetually in the dark.
Synchronously rotating exoplanets seem inhospitable, yet Haberle et al (1994) hypothesised that
an atmosphere of more than 0.1 bar of CO2 could provide sufficient heat transport to prevent
freezing on the unilluminated hemisphere. (Planets on elliptical orbits that intersect the HZ are
not synchronously locked, although they undergo long-term cyclical variations comparable to
those modeled in the experiments discussed in Section 4.2.) More detailed models of
synchronously rotating planets (Joshi et al, 1997; Joshi, 2003) support Haberle et al’s
conclusions. There has been growing interest in habitable M-dwarf exoplanets; in particular, the
SETI NAI team recently convened a series of workshops to investigate relevant issues (PI Reid
and CoIs Chyba and Turnbull are involved in these studies).
An issue of particular interest for M dwarf exoplanets, and directly relevant to the experiments
outlined in Section 4.3, is the role played by high-energy chomospheric and coronal radiation,
generated by the stellar magnetic fields. M dwarfs are proportionately more active than solartype stars, and can emit 10% of their total energy as ultraviolet and X-rays. Many M dwarfs have
the same absolute X-ray luminosity as the Sun; since the conventional HZ lies at ~0.3 AU, an M
dwarf exoplanet is subject to X-ray and particle fluxes ten times that of Earth. Moreover, during
flares, which occur more frequently than on the Sun, the high-energy flux can increase a further
hundredfold. This non-thermal radiation might provide an additional source of heating for
planetary atmospheres, but could also lead to disadvantageous mutations in living organisms.
To what extent does this radiation environment affect planetary habitability, and how do these
emissions change over time? M dwarf activity has two components: an approximately constant
flux from the quiescent chromosphere/corona; and short-lived (minutes to ~few hours) flares,
where the ultraviolet and X-ray fluxes can increase by factors of 102 to 104 over inactive levels.
The NAI JPL/Caltech Virtual Planet Laboratory team is currently modeling some effects due to
quiescent activity for selected M dwarfs; PI Reid is a CoI on a related Hubble Space Telescope
proposal (PI: L. Walkowicz, U. Washington), using the Advanced Camera for Surveys (ACS) to
obtain low-resolution ultraviolet spectra of nearby M dwarfs, measuring the range of quiescent
activity as a function of spectral type. Walkowicz is also measuring H and Ca II H&K
emission-line strengths for these stars, providing coss-calibration of multi-wavelength activity
indicators.
Activity in M dwarfs is likely to vary over a wide range of timescales:
1. there is a long-term decline in activity with increasing age;
2. flares decrease in frequency with age, although there are no reliable determinations of the
time dependence (does it scale with the quiescent flux?) or the variation in the peak flux;
3. there may be short-term (few year) cyclical variations in activity, comparable to the Solar
cycle.
Superimposed on all these variations is a general trend: activity declines at a slower rate with
decreasing mass.
The long term decline in quiescent activity is best studied through observations of stars in open
clusters of various ages. Hawley et al (1999) completed an initial analysis, based on observations
Confidential
Page 27
6/20/2017
of M dwarfs in 6 clusters with ages between 30 million years (IC 2602) and 5 Gyrs (M67). The
sample does not include any clusters with ages between the Hyades (500 Myrs) and M67. The
activity level was gauged by determing the spectral type where H emission becomes apparent.
We propose to extend this age-activity calibration by obtaining optical spectra of M dwarfs in
open clusters with ages between 1 and 5 Gyrs.We aim to use multi-object spectrographs on the
Gemini telescopes, and the KPNO and CTIO 4-meter telescopes for these observations.
Besides long-term changes that occur as stars age, shorter-term activity cycles exist. Those
cycles are well-established for Sun-like stars but are little studied at later spectral types. The
possible existence of cyclical variations in M dwarf activity levels is currently being addressed
by CoI Valenti, who is using SMARTS-consortium time to monitor Ca II H&K emission in
selected neaby M dwarfs. The current observations span two years, and this program will be
continued in the near future.
Finally, while M stars are famous for their high-intensity flares, quantitative measurements of the
variation in flare activity with age remain sparse. While it seems clear that flare frequency must
decline with age, along with quiescent activity, observations tend to concentrate on younger,
more active stars. We propose to address this issue by photometrically monitoring M dwarf stars
in open clusters, concentrating on systems with ages between 1 and 5 Gyrs. These observations
can be made in tandem with searches for planetary transits, with the important proviso that data
are taken at blue wavelengths, to provide maximum sensitivity to flares. The observations will be
obtained and analysed by Reid, Turnbull, Soderblom and collaborator Sahu. We will use the
CTIO telescopes that are part of the SMARTS consortium to study southern clusters in the
appropriate age range.
As results are obtained for these closely related M dwarf projects, the astronomer CoI’s Valenti,
Reid and Turnbull will work with the extremophile CoI’s Robb, DasSarma and Chen to
understand how microorganisms can survive, and even flourish, under M dwarf-like radiation
environments.
3.5 Summary
The principal tasks outlined in this section are:
 Pursue the characterization of exoplanet atmospheres through ground-based and spacebased spectroscopy of transiting exoplanets
 Model the dynamical formation and evolution of exoplanetary systems
 Determine the range and time-variability of the high-energy radiation environments
experienced by M dwarf exoplanets
Confidential
Page 28
6/20/2017
4. Can the exoplanets support life?
4.1 Introduction
Astrophysical environments span a range of harsh conditions. Within the Solar System, average
surface temperatures range from 300°C for Mercury to -230°C for Pluto, with greater extremes
occurring locally and seasonally; many known exoplanets are subject to an even greater range of
conditions (see Figure 3.1). Nonetheless, recent studies of diverse environments on Earth suggest
that some forms of microbial life have adapted to a significantly wider range of conditions than
previously thought possible (Faison, 2003). Besides temperature extremes, these include
anaerobic conditions, denaturing and caustic chemical species, such as salts, hydrogen and
hydroxide ions, heavy metals, etc., and damaging radiation and desiccating conditions
(Rothschild and Mancinelli, 2001). Indeed, some microorganisms thrive in (and even require)
these extreme conditions; such species have been dubbed “extremophiles”. Many are Archaea,
members of life’s third domain, while most prior NAI studies have focused on Bacteria. We have
selected a representative set of terrestrial microorganisms, including cold-adapted and radiationresistant species, that are particularly well suited for further investigation of life’s limits – a
crucial aspect of Goal #5 in the Astrobiology Roadmap.
The COMB team of researchers will target microbial life forms that likely evolved at an early
stage in Earth’s history, and which will provide insight into possible life elsewhere in the Solar
System and on exoplanetary systems (Table 4.1). The Archaea were recognized as a separate
taxonomic group just over 25 years ago (Woese and Fox, 1977; Woese, 1987). They display
relatively simple growth requirements and are thought to be evolutionary relics. Although their
early history is unclear, studies suggest that Archaea are genomic chimeras or hybrids,
containing features of information transfer systems similar to higher organisms, and with
metabolic capabilities similarities to Bacteria. Halophilic Archaea (e.g. Halobacterium species)
contain a simple retinal-based photopigment which may have evolved even earlier than
chlorophyll-based photosynthesis, a hypothesis based on the complementarity of the spectra of
chlorophyll and retinal-based pigments (DasSarma, 2004). Methanogenic Archaea can grow in
the most minimal components, such as H2 and CO2 or CO. Methanogens are capable of growth
in an extremely wide range of temperatures, from below 0oC to a high of 110oC.
Some of the earliest fossil records of life on Earth are found in stromatolites, formed by
microbial communities. We will therefore also investigate species in modern microbial mat or
biofilm communities, including those present in saline, hypersaline, and anxoic environments
(Cohen and Rosenberg, 1989). Significantly, the photosynthetic activities of such populations
contributed significantly to changing Earth’s atmosphere from reducing to oxidizing, altering the
course of evolution and leading to the development of higher organisms. For example,
photosynthetic cyanobacteria, such as Synechococcus spp., contain chlorophyll-based
photopigments similar to plants and may be ideal as founding organisms (Schopf 1993). They
have the capability to grow on minimal chemical components, utilizing the energy of sunlight
and the ability to fix CO2 (the "dark reaction" of photosynthesis). Some purple bacteria, e.g.
Rhodospirillum spp., may grow photosynthetically within anaerobic zones, utilizing H2S as an
electron donor for CO2 fixation, and also autotrophically, via CO oxidation.
Confidential
Page 29
6/20/2017
In selecting individual species for study, our top priorities are representative cold-adapted
halophilic and methanogenic microorganisms isolated from Antartica (see Section 4.2) which we
have studied over the past two years in an inter-institute collaboration (STSCI and COMB).
These have been compared to mesophilic relatives through comparative genomic, biochemistry
and physiological approaches (Table 4.1). The mesophiles are model organisms which have been
completely genetically sequenced, are transformable, and have plasmid vectors and other genetic
tools for use. Our first report on the finding of methanogenesis and biofilm formation at subzero
temperatures has been submitted recently to Astrobiology (see below; Reid et al. 2006).
Table 4.1. Microbial species for probing life’s limits to cold temperature.
Organism type
Experimental
Model Species
Halophilic Archaea Halorubrum
lacusprofundi
Halobacterium
NRC-1
Methanogenium
Methanogenic
frigidum
Archaea
or Terrestrial Habitats Characteristics
relevant
to
astrobiology
Hypersaline
Extremely halophilic, radiation resistant,
Antarctic lake
psychotroph, growth at < 0 oC
sp. Great Salt Lake, Extremely halophilic, radiation resistant,
solar salterns
mesophile, genetically tractable model
Saline,
anaerobic Halotolerant,
strict
anaerobe,
Antarctic lake
methanogenic psychrophile to < 0 oC,
autotrophic growth with H2 and CO2
Methanococcoides Saline,
anaerobic Halotolerant,
strict
anaerobe,
burtonii
Antarctic lake
methanogenic psychtolerant to -2 oC,
growth with simple methylated molecules
Methanosarcina
Saline,
Halotolerant, strict anaerobe, mesophile,
acetivorans
marine sediments
genetically tractable model
Marine sediments
Halotolerant, omniadaptive growth via
Green
nonsulfur Rhodospirillum
rubrum
respiration, fermentation, photosynthesis,
Bacteria
photoautotrophy, autotrophic growth with
CO, tractable model, 5-10oC
Synechococcus spp. Estuarine, coastal, Photosynthetic, autotrophic with CO2,
Cyanobacteria
CB0101, CB0205
open ocean
radiation resistant, highly diverse and
ancient microbes, tractable, growth limits
not yet tested
In addition to studying the mechanisms of cold-adaptation of currently cultured organisms, we
will also prioritize new microbial enrichments from Arctic, subarctic, and Antarctic Mars analog
sites by working closely with our collaborators on this proposal and with other NAI teams. This
work is likely to expand our breadth of understanding of species diversity and metabolism
operating in the cold. Finally, we will also study radiation-tolerance of microorganisms, which is
likely to serve as a significant advantage for survival on planets subjected to high radiation
intensities, including Mars, and for possible dispersal of life in our galaxy. These studies will be
particularly relevant from the standpoint of planetary protection, especially for Mars. In
summary, our strategy will permit us to probe the limits of life on Earth as well as define the
relevant physiological and genomic mechanisms operating under stress. These priorities include
selected collaborations with scientists at other NAI nodes which will complement and extend our
work and enhance the overall NAI effort in extremophile biology.
4.2 Life in the Cold
From the human perspective, Earth’s environment is clement. However, 80% of the biosphere
never experiences temperatures higher than 5oC. In the Solar System, Mars and Europa, the two
Confidential
Page 30
6/20/2017
best candidates for life, both experience average temperatures well below 5oC, as do many
exoplanets (Figure 3.1). Cold environments are commonplace throughout the Galaxy. In
addition, surface temperatures are not stable over time; extreme temperature variations occur on
a diurnal and seasonal basis as well as with longer-term periodic or chaotic changes in orbital
parameters (e.g., Mars’s obliquity). Therefore, thermal cycling events have a significant impact
on predictions of survival. In our own Solar System, for example, freeze-thaw cycles in the
Martian near-subsurface have been cited as one of the strongest challenges to the survival of
terrestrial bacteria that may be brought to Mars on Earth spacecraft (National Research Council
2005).
On Earth, Antarctica has one of the driest and coldest habitats and provides scientists with a
diverse inventory of cold-loving (psychrophilic) and cold-tolerant (psychrotolerant) organisms.
Such microorganisms represent highly specialized and frequently highly localized microbial
biotypes, including those associated with ice-covered and permanently liquid lakes, glacial and
sea ice, and a wide range of soils and rocks. They are characterized by one or more
environmental extremes (including low temperature, wide temperature fluctuations, desiccation,
hypersalinity, high periodic radiation fluxes, and low nutrient status). Several species of
halophilic and methanogenic Archaea isolated from the Vestfold Hills, including Halorubrum
lacusprofundi, Methanogenium frigidum Ace-2 and Methanococcoides burtonii, are cultured in
our laboratories and serve as genetic and physiological experimental systems. These species
serve as comparative partners to mesophiles from diverse environments (see Table 4.1).
4.2.1 Cold and salt: Psychrophilic halophiles
The freezing temperature of pure water at terrestrial standard atmospheric pressure (101.3 kPa) is
0oC. By contrast, brine-saturated water can exist in liquid form below –20oC and other salts can
depress the freezing point to –50oC or lower. A number of hypersaline lakes have been studied
from Antarctica, including Dry Valley lakes (Lake Bonney, Lake Vanda, and Don Juan Pond)
and Deep Lake (in the Vestfold Hills) (Javor, 1989). Some lakes contain greater than 25 % total
dissolved salts. Several extraterrestrial bodies have saline environments, notably Mars and
Europa, so the study of salt-tolerant microbes is highly relevant to the aims of our project.
Halorubrum lacusprofundi is a halophilic Antarctic species isolated from Deep Lake
(Franzmann et al., 1988). This is an aerobic species reported to be capable of growing at 2-4oC,
although recently shown to grow at -1 oC in our COMB-STSCI collaboration (Reid et al. 2006).
Thus, it can survive and divide at sub-zero temperatures in essentially saturating concentrations
of NaCl. Phylogenetically, H. lacusprofundi is a member of the halophilic Archaea, and contains
transcription and DNA replication systems similar to higher organisms. This archaeon is highly
pigmented and contains the light-driven proton pump, bacteriorhodopsin, used for phototrophic
growth. A draft sequence of the genome of H. lacusprofundi has been determined in the
DasSarma laboratory and is currently being completed through DOE’s Community Sequencing
Program at the Joint Genome Institute (JGI). DasSarma and collaborator R. Cavicchioli,
University of New South Wales, are CoPIs on this project and the complete sequence will be
available to us soon, six months before public release in late 2006.
A similar but mesophilic haloarchaeon with a growth range of 15-55oC is Halobacterium sp.
NRC-1 (DasSarma, 2004). This microbe is found in the Great Salt Lake and other evaporitic
Confidential
Page 31
6/20/2017
hypersaline environments that are characterized by salinity levels of 3-5 M NaCl and intense
solar radiation. Halobacterium sp. NRC-1 is a model organism studied for 20 years in the
DasSarma laboratory, which is genetically tractable and for which a host of post genomic
methods, such as directed gene knockouts, DNA microarrays and proteomics, have been
developed. The response of Halobacterium to changes in salinity, temperature, nutrients, light,
radiation and oxygen have been studied (Müller and DasSarma, 2005; McCready et al. 2005) and
will provide an exceptionally good mesophilic relative for comparison to H. lacusprofundi.
Specific aims: (1) To determine the low temperature limits to growth of the psychrophilic
halophile, H. lacusprofundi in comparison to its mesophilic relative, Halobacterium sp. NRC-1.
(2) To examine the mechanisms for survival of haloarchaea to environmental dynamics (e.g.
freeze-thaw cycles, desiccation-hydration cycles) through physiological and genomic
approaches.
Experimental plans: The effect of temperature on growth dynamics will be assessed through
growth in a variety of media (DasSarma and Fleischmann, 1995), experiments already initiated
by CoI DasSarma and collaborator J. Coker at COMB. Initially, nutrient content will be
increased to the maximum permissible by solubility, which should enhance growth and depress
freezing points. Alternatively, other salts and water miscible organic solvents will also be tested.
Finally, a combination of nutrients, salts, and solvents will be used to approach the low
temperature growth limit. We will use liberal criteria for assaying growth, given the time scales
appropriate to growth on exoplanets and our recent observation that cultures may adapt to colder
temperatures over time (Reid et al, 2006). We will monitor cultures over a 3-year time period.
Once the lower limit of growth is determined, an additional goal will be to determine the extent
of survival at temperatures sub-inhibitory for growth (Franzmann et al. 1988). We wish to
explore whether organisms may survive for extended periods of time when temperatures are too
low for growth, allowing return to active growth when temperatures and other conditions permit.
This is an important goal given the orbital ellipticity of many exoplanets, which may result in
long winter seasonal cycles. What temperature extremes are tolerable without complete loss of
viability? How long do cells survive after freezing and how many cycles of freeze-thaw may be
tolerated? A multiyear time-line will be followed, allowing a thorough comparative study of the
survival of these haloarchaea. Analysis of both psychrophile and mesophile will allow us to
determine if the former has devised specific mechanisms to permit survival in quiescent state for
an extended period of time.
In addition to establishing the effect of freeze-thaw, we also plan to carry out desiccationhydration cycles to determine the capability of the halophiles to withstand such stresses (Landis,
2001). Although halophiles have been reported from ancient salt deposits (Vreeland et al. 2000),
their survival over multiple rounds of desiccation and rehydration has not been studied
(Kotteman et al. 2005). No spores have ever been observed in this class of microorganisms, so it
is likely that novel strategies have been devised to allow survival in arid environments.
Once the parameters of survival and death have been defined, we plan to address the underlying
mechanisms through a genomic approach. A whole genome oligonucleotide microarray has
already been used successfully for the mesophile (Müller and DasSarma, 2005; McCready et al.
Confidential
Page 32
6/20/2017
2005) and a similar microarray will be constructed for the psychrophile (see Section 4.2.4.1).
Microarrays provide a powerful tool that, in combination with physiological approaches
described above, will address the biological responses relevant to astrobiological conditions.
The regulated genes will be compared between the two strains and will provide the basis for
understanding the mechanism of adaptation, besides suggesting possible gene transfer
experiments (see Section 4.2.4.2).
4.2.2 Cold and anoxia: Psychrophilic methanogens
Psychrophilic methanogens represent life forms that exist in perennially cold, anoxic conditions
with minimal nutrient requirements. Methanogens use hydrogen and hydrogen rich compounds
and carbon dioxide to produce energy and release methane. Knowledge of the adaptation
mechanisms for growth and survival of these microorganisms will provide insight into the
extreme limits of life that might arise in cold exoplanets with oxygen-free atmospheres.
Genome sequencing of the psychrophilic methanogen, Methanogenium frigidum (theoretical Tmin
–1.8oC) and the psychrotolerant methanogen, Methanococcoides burtonii (Tmin 1.7 oC) was
recently initiated. These archaeal species were isolated from a perennially anoxic zone of Ace
Lake in Antarctica. Constant temperatures of 1 to 2oC, salinity of up to 4.3 % and high
concentrations of methane and H2S further characterize this region of the lake. The ability to
grow at low temperatures, in the absence of oxygen and with only hydrogen or simple organic
compounds, make these microorganisms particularly relevant for assessing habitability, since
these conditions are associated with the early formation of the Earth’s biosphere.
This study combines physiological and genomic data to identify survival characteristics that can
constrain the diversity of microbial species expected in cold exoplanets. Completing the M.
frigidum and M. burtonii genome sequences will expand our understanding of the organismal
and metabolic biocomplexity of cold-adapted Archaea, and provide the reference for comparing
mechanisms of archaeal cold-adaptation with those in Bacteria and Eukarya. Completed genomic
sequences combined with proteomic and genetic approaches will be used to characterize the
absolute limits of adaptation and identify cold-adaptation mechanisms.
A
Specific aims: To determine the lowest temperatures that support growth and/or methanogenesis
by these microorganisms, the longevity of these microbes at temperatures below the growth
range, and the range of temperature fluctuations (freeze-thaw) under which they can survive. To
elucidate mechanisms by which survival under these conditions takes place, we will investigate
the physiological and genetic factors that limit growth at the lowest temperatures.
Experimental plans: To date there have been only two reports (Franzmann et al, 1992; 1997)
describing the physiological characteristics of M. burtonii and M. frigidum. Although both
suggested that the archaeal species grew sub-optimally at their in situ temperature in the
laboratory, CoI Sowers showed that M. burtonii growth and methanogenesis occur at sub zero
temperatures (Reid et al, 2006). We will continue adaptation studies with M. burtonii and
initiate adaptation studies with M. frigidum to determine the lowest temperature for both growth
(cell doubling) and methanogenesis (physiological activity). Both species will be maintained and
monitored at –15 to 0 oC using refrigerated water bath shakers containing antifreeze. As the
marine media for both species freezes at -5 oC we will add a cryoprotectant (such as glycerol) to
Confidential
Page 33
6/20/2017
prevent freezing. We will also test the ability of frozen cultures to recover after storage in
medium without cryoprotectant for different periods of time and we will also test the efficiency
of recovery for cultures after repeated freeze-thaw cycles. The ability of these species to
maintain metabolic activity at subfreezing temperature will be determined by monitoring
methanogenesis over time in frozen cell suspensions. Overall the experiments will define the
limits for (1) growth, (2) metabolic activity and (3) recovery after stasis.
4.2.3 Cold and Carbon Monoxide
The simplest autotrophic growth requirements on record are provided by the Bacteria and
Archaea that grow in the dark under anaerobic conditions with CO as the sole source of carbon
and energy. This is accomplished via the gas-shift reaction, CO + H2O ↔ CO2 + 2 e- + 2 H+. The
key enzyme in this process is carbon monoxide dehydrogenase (CODH) that catalyzes the
biological oxidation of CO at an unusual Ni-Fe-S cluster called the C-cluster (Drennan et al.
2001) using this 2-electron, hydrogen producing process. We will study , Rhodospirillum
rubrum, capable of growth with CO alone and at temperatures as low as 5-10 oC. We will
contrast this species with another CO oxidizer, Carboxydothermus hydrogenoformans. These
strains are distinct in their taxonomy and physiology: R. rubrum is a mesophilic, Gram negative,
purple nonsulfur bacterium that can grow aerobically or anaerobically. It is exceptionally
versatile and has the ability to live through photosynthesis, cellular respiration, fermentation, or
chemoautotrophic growth. In contrast, C. hydrogenoformans is an extreme thermophile, able to
grow rapidly on high levels of CO and grows poorly on a very limited assortment of substrates.
We recently completed the genome sequence of this low GC, Gram positive bacteriun, and found
that it has five genetic loci encoding CODH, one of which is remarkably similar to the single cdh
locus in R. rubrum (Wu et al, 2006)
Anaerobic CO oxidation may be a model for microbial growth in primitive planetary
atmospheres (Weiss et al, 2000). CO is the second most abundant molecule, after water, in the
ice mantles of interstellar grains (Ehrenfreund, 1999, Charnly et al. 2001) and thus the study of
terrestrial organisms that specialize in CO oxidation is astrobiologically relevant. The chosen
representatives have a draft genome sequence from the JGI, in the case of R. rubrum, and a
newly published, complete genome sequence, in the case of C. hydrogenoformans (Wu et al, in
press). These will provide the basis for comparative studies on the thermodynamics of metabolic
pathways providing energy and carbon fixation from CO oxidation.
Specific aims: The free energy (G) of CO oxidation is highly temperature dependent, and we
will carry out enrichment and isolation of new psychrophilic CO-oxidizing strains and compare
their growth yields and mass balances with the mesophilic and thermophilic model organisms.
Experimental plans: CoI Robb and collaborator A. Colman will determine the minimum
growth temperature of R. rubrum in batch cultures and chemostats, and test a wide range of
growth temperatures, with a range of partial pressures of CO in the headspace. Mass balances for
the cultures will be determined by measurement of biomass produced and through gas
chromatographic headspace analysis to determine CO consumed and H2 produced. We will
determine the adaptive response of the CO dehydrogenase encoding loci in R. rubrum and C.
hydrogenoformans by transcriptional profiling, using microarray analysis. Spotted DNA
microarrays have been developed in collaboration with Idaho National Laboratory. We will
Confidential
Page 34
6/20/2017
enrich from samples in Mars analog sites for psychrotolerant and psychrophilic CO oxidizing
cultures and isolate pure cultures, in collaboration with collaborators Cavicchioli, Onstott, Steele
and Fogel. We may find obligate consortial partnerships between CO-oxidizing bacteria and
methanogens, and will propagate these systems under CO headspaces and determine species
composition by 16SrRNA sequencing. Our NSF funded Microbial Observatories site in the Uzon
Caldera, Kamchatka, also has permafrost sampling sites. Recently several methanogens have
been grown on CO as carbon and energy source, and we will attempt to enrich for psychrophilic
methanogenic consortia as well as acetogens from cold sediments. We will also carry out
fluorescent in situ hybridization (FISH) to reveal interspecies juxtaposition in the consortia.
Isolates will be subject to mass balance determination to test their efficiency of CO utilization,
methanogenesis, acetogenesis and hydrogen production.
4.2.4 Mechanisms of cold adaptation
The low temperature growth capability of M. burtonii and H. lacusprofundi reflects the genomic
composition of these highly adapted microorganisms. Since partial genome sequences are
available, we have conducted an inventory of predicted proteins known to be important for
coping with low temperatures. Strikingly, the genome of H. lacusprofundi contains 5 copies each
of the two different cold shock protein (CSP) genes while the mesophilic Halobacterium sp.
NRC-1 contains only one copy each (cspD1 and D2). In general, CSPs are involved in various
cellular processes and believed to enable cells to adapt to low temperature (Thieringer et al.
1998). They can bind to single stranded DNA and RNA and have been suggested to function as
RNA chaperones facilitating the initiation of translation under optimal and low temperatures.
Overproduction of CSPs leads to increased survival during periods of freezing (Wouters et al.
2000) and the presence of multiple copies of CSP genes in H. lacusprofundi suggests a molecular
basis for the cold-adapted nature of this organism.
Figure 4.1 Scanning electron micrographs of cell aggregates formed by H. lacusprofundi grown at 4 oC (A)
and Methanococcoides burtonii grown at –2.5 oC (B). The bars represent 2 m.
Comparative analysis of the incomplete genomes of the psychrotolerant M. burtonii and the
psychrophilic M. frigidum, also isolated from Ace Lake, revealed several potential mechanisms
(Saunders et al., 2003). A gene with high homology to the cold shock domain (CSD) protein
cspA was detected in the genome of M. frigidum. In contrast cspA was not detected in M.
burtonii, but two proteins were detected that contain the highly conserved CSD fold
characteristic of CSPs. Another unique characteristic of the cold adapted methanogens is the
Confidential
Page 35
6/20/2017
amino acid composition of proteins, which generally increase exposure of hydrophobic residues
and decrease charge. This modification was suggested to decrease the activation energy by
increasing protein flexibility.
Cell aggregation might be a common high temperature-dependent stress response among
Archaea (Hartzell et al., 1999). In bacteria, this is a typical response to stress conditions.
Interestingly, we observe extensive cell aggregates of both H. lacusprofundi and M. burtonii
when cultures are incubated in the cold (Figure 4.1). This is of particular interest since the
formation of a multicellular complex, comprised of archaeal, bacterial, or eukaryotic cells,
constitutes one of the most fundamental aspects of developmental biology. Cell aggregation may
facilitate exchange of nutrients, membrane components, and genetic material, thus enabling the
organisms to cope with a stressful environment.
4.2.4.1 Biofilm studies
Many microorganisms, including Archaea, are present in microbial mats found in hypersaline
environments (Spear et al. 2003), anaerobic methane oxidation systems (Boetius et al. 2000;
Orphan et al. 2001; Hallam et al. 2004) or acid mine drainage (Tyson et al. 2004). The latter two
examples addressed interactions between members of the domains Archaea and Bacteria using
environmental genomics. Archaea-containing biofilms are also important in industrial
applications (Hulshoff Pol et al., 2004; Zhang et al., 2003), and in the hindguts of many
terrestrial arthropods (Eckburg et al. 2003). However, knowledge about molecular details of
biofilm formation in the Archaea is sparse. Means for surface attachment may involve unique
flagella that have structural similarities to the type IV pili of bacteria (Thomas et al. 2001) as
well as other proteins predicted to be involved in fibril formation and surface adherence
(Kachlany et al. 2000). Various exopolysaccharides, key components of biofilm-matrices, are
synthesized by halophilic and thermophilic Archaea (Rinker and Kelly, 2000). Mainly due to a
lack of an appropriate model organism, many important aspects such as cell-to-cell
communication and intracellular signaling pathways in archaeal biofilms have not been
addressed so far. As these are fundamental to microbial life on Earth, it is likely that microbes
on other worlds will also exploit identical or similar strategies for survival.
Our recent experiments demonstrate enhanced biofilm formation for two psychrophiles, H.
lacusprofundi and M. burtonii. Since biofilm formation by microbial cells is a genetically
programmed process (Lazazzera 2005), microarrays are particularly promising tools to gain
insights into the underlying mechanisms. Metabolic pathways of biofilms can also be explored
based on proteomic approaches. A community based proteomics has been applied to investigate
the microbial functions associated with acid mine drainage bacterial biofilm (Ram et al. 2005),
and bacterioplankton in the estuarine water (Kan et al. 2005).
Specific aims: (1) Address cold-adaptation and biofilm formation during growth at low
temperatures by recording the transcriptome of H. lacusprofundi and M. burtonii using a time
lapse. (2) The extracellular matrices will be purified and chemical composition determined.
Experimental plans: We plan to use in situ synthesized oligonucleotide arrays for both H.
lacusprofundi and M. burtonii, using ink-jet technology (Hughes et al. 2001). We previously
used this technology successfully for transcriptome analysis of DMSO/TMAO respiration and
response to UV irradiation in Halobacterium sp. NRC-1 (Müller and DasSarma, 2005;
Confidential
Page 36
6/20/2017
McCready et al., 2005). Oligomer (60-mer) probes will be designed for all ORFs utilizing the
program OligoPicker (Wang and Seed, 2003). The 11,000-feature capability allows for design of
multiple probes per ORF with similar mean Tm and narrow range (<3C) as well as ample
negative and positive control spots to test hybridization conditions. A hallmark of in situ
synthesized oligonucleotide arrays is the low occurrence of data outliers due to non-uniform spot
morphology or background noise. Features replicated within a single array show low differences
in absolute processed signal intensities and spot-to-spot variation for replicate experiments.
Microarray experiments will be conducted in collaboration with J. Müller, Morgan State
University. The genome sequence of M. burtonii is currently being constructed by DOE-JGI in
collaboration with CoI Sowers (see http://genome.jgi-psf.org/) and closure is estimated within 6
months. A microarray will be constructed and parallel studies will be conducted with the
methanogen to identify genes expressed in response to cold growth.
Employing oligonucleotide microarrays, we expect to gain knowledge of the mechanisms of
cold-adaptation in H. lacusprofundi and M. burtonii on several levels. (i) A principal analysis of
the effect of cold temperature regimes on the transcriptome this organism will occur. Additional
temperature-dependent regulatory networks and promoter analysis will be supported by
comparative genomics. (ii) An insight into gene activity on a global scale will be obtained.
Averaged signal intensities of probes are approximately proportional to the relative transcript
level of the corresponding gene. For example, the genome of H. lacusprofundi contains ca. 10
csp genes, and we will be able to address hierarchy in importance of the CSPs. (iii) The
comparison with existing microarray data on cold-adaptation in Halobacterium sp. NRC-1 will
reveal what is unique to H. lacusprofundi in its response to cold temperatures. Is there a subset of
genes that is present in both organisms but differentially expressed only in H. lacusprofundi? Or
are there genes differentially expressed in H. lacusprofundi that are not present in Halobacterium
sp. NRC-1? Similar comparative analysis may also be conducted for the psychrotolerant
methanogen M. burtonii and the mesophilic methanogen, and M. acetivorans.
A second avenue of studies will address biofilm formation in H. lacusprofundi and M. burtonii
during growth at low temperatures. Since biofilm formation by microbial cells is genetically
programmed, microarrays are particularly promising tools to gain insights into the underlying
mechanisms. Thus far, no report on global gene expression changes associated with biofilm
formation in Archaea has been published. We plan to record the transcriptome of H.
lacusprofundi and M. burtonii during different times of biofilm formation. Incorporating several
points is of particular importance since there is no clearly defined ‘endpoint’ in biofilm
formation (Lazazzera 2005). The experimental approach will be similar to that previously
established for various Bacteria (Beloin et al. 2004, Pysz et al. 2004, Resch et al. 2005). Cells
grown in biofilms will be compared with planktonic cells from the same cultivation vessel as
reference. We expect to identify the subset(s) of genes important for biofilm formation and
maintenance, and gain an insight into the regulation of those genes. We are aware that cell
populations in biofilms are rather heterogeneous, which may complicate data analysis.
We plan to study the composition of extracellular matrices visible in biofilms of H.
lacusprofundi and M. burtonii (and possibly other psychrophilic microbes), with the goal of
identifying their chemical composition. Initial results indicate that a polysaccharide is present, at
least for H. lacusprofundi, based on Congo Red staining (Allison and Sutherland, 1984). Similar
Confidential
Page 37
6/20/2017
studies will be conducted with M. burtonii to determine if analogous mechanisms may be used in
both of these psychrophiles. For further analysis extracellular matrices will be purified by via
tangential-flow filtration (10,000-molecular-weight cutoff) and precipitation and size-exclusion
chromatography and subjected to enzymatic and mass spectrometry analysis (Maira-Litran et al.
2002; Vuong et al. 2004).
4.2.4.2 Gene transfer and complementation studies
Different groups of microorganisms exhibit distinct types of cold temperature responses (Inouye
and Phadtare, 2004). For example, E. coli responds to low temperatures by adjusting its
membrane lipid composition and increasing the production of both CSP proteins and proteins
that facilitate ribosomal assembly. In contrast, yeast responds with increases in the synthesis of
trehalose and a heat shock protein. Genomic analysis of H. lacusprofundi and M. frigidum Ace-2
has identified stress proteins that are likely to be important for survival of these species in the
cold, including a number of cspD homologs (our unpublished data). Our strategy is to identify
genes that can promote cold growth and survival by gene transfer from the psychrophilic to the
mesophilic species. For the halophile, we plan to use the model, Halobacterium sp. NRC-1, as
host, while for the methanogen we will use M. acetivorans. Both mesophilic species have welldeveloped genetic systems in use in our laboratories as well as complete genome sequences,
making them ideal for complementation analysis.
Specific aims: (1) To examine what protection cspD genes provide to cells exposed to low
temperatures. (2) For complementation analysis, use additional genes identified through
bioinformatic and microarray studies, e.g. anti-freeze protein genes and lipid biosynthetic genes,
which may confer the ability for cold growth.
Experimental plans: We plan to overexpress a selection of cold-active genes identified in the
psychrophiles in the mesophilic model counterparts to address and confirm the involvement of
those genes in cold adaptation. Initially, we will overexpress the CSP genes of the psychrophiles
in both organisms. A previously constructed and tested overexpression vector, pKJ408 (Jung and
Spudich, 1998), will be used for the halophile system in the DasSarma lab. In pKJ408, the
cloned gene will be under control of the ferredoxin promoter from Halobacterium sp. NRC-1 to
enable moderate overexpression. In addition, pKJ408 harbors a mevinolin resistence gene, mevR,
for selection. PCR primers will be designed for amplification of the respective gene plus
introduction of appropriate restriction enzyme sites (NdeI and XbaI). PCR-amplified genes will
be restriction digested and ligated into restriction-digested pKJ408. The resulting vector will be
amplified in E. coli and subsequently transformed into H. lacusprofundi and Halobacterium sp.
NRC-1 essentially as described (DasSarma et al. 1995). Successful overexpression will be tested
for by semi-quantitative Reverse-Transcriptase-PCR and by standard SDS-PAGE protein
analysis. If required, a new vector will be constructed in which the ferredoxin promoter from
Halobacterium sp. NRC-1 will be substituted with that from H. lacusprofundi. The physiological
response of either organism after successful CSP expression will be tested by standard growth
tests. Subsequently, we intend to express additional genes after they have been implicated to be
involved in cold adaptation.
The effects of CSP in the mesophile will be tested by expressing the gene in a plasmid-based
expression system developed by CoI Sowers for Methanosarcina acetivorans. The vector pSM1,
Confidential
Page 38
6/20/2017
a derivative of pWM315 (Metcalf et al, 1997), contains a sequence of the native plasmid from M.
acetivorans, p2CA, to promote autonomous replication in M. thermophila -pir dependent R6K
origin of replication for amplification in E. coli. The parent plasmid also contains the puromycin
resistance gene (pac), which confers puromycin resistance for selection in M. thermophila and
the gene for -lactamase for ampicillin resistance in E. coli. The CSP will be cloned downstream
of the promoter for the gene encoding CO dehydrogenase in M. acetivorans, which is expressed
in cells grown with acetate. If flanking restriction sites are not compatible with the multiple
cloning site of the expression vector, compatible sites will be inserted by PCR-mediated
mutagenesis of one or both flanking regions. The construct will be transformed into E. coli and
the cloned gene will be confirmed by restriction analysis and sequencing. The expression vector
will then be transformed into M. acetivorans and confirmed transformants will be tested for
extended tolerance to low temperatures. This system has already been shown to effectively
express other archaeal proteins (Sowers, manuscript in preparation).
4.2.4.3 Ecological and proteomics studies
A substantial fraction of Earth’s biosphere undergoes seasonal freeze-thaw cycles. Among the
diversity of cold adapted bacteria found in these environments, the genome-based study of
Colwellia psychrerythraea (Methe et al. 2005) recently showed that cells undergo a collection of
synergistic changes when growing in cold condition, which include DNA and amino acid
composition, cell membrane fluidity, uptake and synthesis of compounds conferring
cryotolerance, and cold-adapted enzymes for carbon uptake.
A significant priority will be increasing our understanding cold-adaptation of microorganisms.
For this goal, we plan to expand our collection of cold-adapted species and consortia with
samples from the study sites of Co-I Robb, whose Microbial Observatories funded project in the
Uzon Caldera, Kamchatka Peninsula has permafrost locales on the rim of the caldera. Also,
samples will be collected in the Svalbard Mars analog study site via collaborators Fogel and
Steele of the CIW NAI, and additional polar sites, in the Antarctic Vestfold Hills and Ace Lake
(via our collaborator R. Cavicchioli at the University of New South Wales). Additional studies
with collaborator Onstott will focus on a deep permafrost site in Nunevat Territory, Canada as a
Mars analogue site where we will be able to obtain permafrost core and saline brine to a depth of
0.5 to 1.1 kilometers. These samples will be enriched using the microbiology procedures we are
developing for the model organisms, including growth under progressively declining temperature
regimes to determine the Tmin for growth (Sowers et al, in preparation). We will compare and
contrast new isolates with the physiological types we are studying, and the existing culture
collection at COMB.
CoI Chen has compiled a large collection of bacterial strains (incluing Synechococcus spp.
CBO101 and CBO205) from the Chesapeake Bay, isolated during the winter season at 0 oC, as
well as during warmer seasons (Kan et al. in press). Most bacterial strains isolated in winter are
distinct phylogenetically from summer isolates, and many are closely affiliated with isolates
from colder and permanently cold environments including polar seas, Arctic sea ice, Antarctica,
Baltic Sea etc. Since the Chesapeake Bay isolates are adapted to the winter surface water which
may sometimes be frozen, their cold adaptation mechanism is of significant interest. Comparison
between these isolates from a temperate climate with those from Kamchatka Peninsula, Svalbard,
and from Antarctica will provide information of the similarities and differences in cold-adapted
Confidential
Page 39
6/20/2017
microorganisms. A combination of culturing and comparative proteomics will be used to explore
the major proteins responsible for psychrophilic activities.
Specific aims: (1) Environmental isolates will be tested for growth at the lowest possible
temperatures. (2) Winter isolates from temperate climate will be compared to those from
permanently cold Arctic and Antarctic environments. (3) Physiological responses to low
temperature at the protein level will be investigated using proteomics.
Experimental plans: All environmental samples will be stored and transported frozen to CoI
laboratories at COMB (Chen, Robb, Sowers, and DasSarma) under appropriate conditions.
Bacterial strains (over 200 strains) previously isolated from winter and summer expeditions in
the Chesapeake Bay are currently preserved at –80oC. These strains will be cultivated at different
temperatures to test for the lowest possible temperature for growth, as described in sections
4.2.1-3. Meanwhile, bacterial strains from indigenous Arctic and Antarctic environments will
also be tested to compare the psychrophilic activities between temperate and polar environments.
A model enzyme, glutamate dehydrogenase, will be assayed at a wide range of temperatures as a
reporter of the temperature optimum of the proteome. This will greatly expand our availability
of cold-adapted species of microorganisms for understanding mechanisms of cold adaptation.
Comparative proteomics will be exploited to understand the key proteins corresponding to the
psychrophilic activities. Proteomics and comparative proteomics have been applied to explore
expressed proteins from the whole organism (Blackstock and Weir 1999, Lopez 1999) or whole
community (Kan et al. 2005, Ram et al. 2005). Unique proteins produced in response to a
specific environmental change can be identified by comparing the protein expression patterns
between control and treatment. CoI Chen and DasSarma routinely use 2-D gel based proteomics
in our labs at COMB and additional expertise is available through collaborator R. Cavicchioli’s
lab at USNW and the UMB Proteomics Core facility. Briefly, cultures grown at different
temperatures will be harvested by centrifugation. Protein extraction, 2-D gel electrophoresis,
image analysis, protein spot excision and characterization will follow the protocols described by
Kan et al. (2005). Application of proteomics permits study of the physiological and biochemical
reaction of bacteria under the tested environmental conditions. Genomes of several bacteria
included in this proposal have been sequenced, making comparative proteomics more feasible.
Initial efforts will be devoted to setting up the culture systems for psychrophilic microbes, since
they grow slowly. Subsequently, we plan to conduct the environmental proteomics studies in
order to understand mechanisms of cold adaptation.
4.3 Radiation tolerance, species survival and meteoric transfer
Mars and Europa, the two likeliest candidates for extraterrestrial life in the Solar System, possess
low-pressure atmospheres, which offer little protection against desiccation and high-energy
irradiation. Moreover, meteoritic transfer of material between Mars, Earth and the Jovian system
has taken place over the lifetime of the Solar System (McGenity et al, 2000; Mileikowsky et al,
2000; Melosh, 2003). There have been suggestions that life could have originated on the more
benign environment of a young wet Mars, and then contaminated a young Earth (interplanetary
panspermia). Indeed, interstellar transport may be possible in young star clusters (see Section
4.3.2).
Confidential
Page 40
6/20/2017
At the present time, the Sun is a relatively inactive star, but exoplanets in other systems are likely
to be subject to a more potent flux of high-energy radiation, while the Sun itself was more active
at a younger age. The diversity of organisms that can survive low-pressure conditions is crucial
for addressing the potential for life on Mars-like planets with low-density atmospheres, life on M
dwarf exoplanets (see Section 3.4), the viability of interplanetary seeding and the potential for
established ecosystems to recover from short-term exposure to high-energy radiation.
4.3.1 Radiation sensitivity of extremophiles
Radiation-tolerance of microorganisms is likely to serve as a significant advantage for survival
on planets subjected to high radiation intensities and is also relevant from the standpoint of
planetary protection, especially for Mars (e.g., Cockell et al, 2000). It also factors into the
feasibility of panspermia mechanisms. Several microorganisms are known to be radiation
resistant, a trait usually correlated with desiccation resistance. Examples include both
thermophilic and halophilic Archaea as well as photosynthetic cyanobacteria. Depending on the
species and strain, resistance may be observed to UV light (including the most damaging UV-C)
as well as high-energy radiation (including gamma radiation and beta-particles). For some
species, LD37 levels may be greater than 200 J/m2 for UV radiation and 10K Gy for high-energy
radiation. While DNA repair processes of common laboratory species, such as E. coli and yeast,
are well studied, the repair mechanisms of extremophilic species are largely unknown.
We (CoIs DasSarma, Robb, Chen and collaborators) are interested in the mechanistic basis of
radiation resistance in several extremophiles, including the halophilic species, Halobacterium
NRC-1, and the thermophile, Pyrococcus furiosus. These organisms display somewhat different
genomic features and resistance mechanisms. For the halophile, genomic studies have shown
nucleotide excision repair systems related to both bacteria (e.g. UvrACBD exonuclease) as well
as the eukaryotic type (Rad2, 3, 25, etc.) (DasSarma et al. 2001). A combination of whole
genome microarray studies (McCready et al., 2005) and gene knockouts shows that both systems
are in use. Moreover, DNA repair likely involves induction of homologous recombination
systems, which may allow repair of double stranded breaks and DNA replication arrest points.
The repair mechanisms of P. furiosus are interesting since this archaeon grows optimally at 100
o
C and is unlikely to encounter ionizing or UV radiation during normal growth (DiRuggiero et al,
1998; Saffary et al. 2002). However, constant growth at or above 100 oC induces both doubleand single-strand breaks into DNA in vivo, and we recently found that heat shock per se induces
DNA repair capacity, and several important DNA repair enzymes, such as the recombinase
radA. The importance of radA induction has also been observed in Halobacterium sp. NRC-1
after UV irradiation (McCready et al. 2005).
Cyanobacteria must cope with the negative effects of ultraviolet stress caused by their obligatory
light requirement for photosynthesis. They may have been present 3.5 billion years ago (Schopf
1993), suggesting that they were able to live in an O2 deficient but UV intense environment.
Some cyanobacteria contain effective radiation repair mechanisms such as synthesis of UVabsorbing scytonemin, secretion of copious extracellular mucilage, phototactic motility and
adherence to substrates. Photoautotrophic cyanobacteria can serve as a model organism for
studying the tolerance of radiation (Hader et al. 1998, Ehling-Schulz and Scherer 1999). Repair
mechanisms can take the form of DNA repair (Blakefield and Harris 1994) or de novo synthesis
of D1 and D2 proteins, the key proteins for repair of UV-B-induced damage in photosystem II
Confidential
Page 41
6/20/2017
(PSII) (Vass et al. 1999, 2000). A freshwater cyanobacterium, Synechococcus sp. is able to resist
UV-B radiation by switching to two other forms of D1 proteins (Campbell et al. 1998). Many
marine Synechococcus strains with different pigment types have been isolated and maintained in
the Chen lab; selected Synechococcus strains will be used for radiation tolerance experiments.
Specific aims: (1) Select mutants with increased high energy-radiation tolerance level for
Halobacterium sp. NRC-1, Pyrococcus furiosus, and marine Synechococcus. (2) Characterize
their physiological and molecular responses to the UV radiation.
Experimental plans: To better understand the basis of radiation tolerance, we will select
derivative strains with increased high energy radiation resistance. Preliminary studies conducted
using the LINAC facilities available at the Idaho Accelerator Center (IAC) with the halophile,
Halobacterium sp. NRC-1, show that mutants of that species are more highly resistant than
Deinococcus radiodurans (DeVeaux et al. 2005). Linda DeVeaux at the IAC will serve as our
collaborator for these experiments. Stationary phase cultures of Halobacterium sp. NRC-1, P.
furiosus, and marine Synechococcus will be exposed to increasing doses of 18-20 MeV electrons
from a pulsed LINAC. Survivors will be regrown to stationary phase and subsequently
irradiated through multiple cycles. Dose-dependent survival for isolates will determined by
comparing colony-forming units of irradiated cultures to unexposed cultures. Similarly, we have
also recently determined that P. furiosus has an increased survival rate at very high fluxes of 
radiation from a Co60 source after sublethal heat shock; we will repeat these experiments with
pulsed 18-20MeV electrons, comparing survival in cultures under optimal growth conditions
(100oC) and heat shock (60 minutes at 105oC). Viability after irradiation will be determined by
end point dilution assay (DiRuggiero et al, 1998). The objective will be to determine the role of
the heat shock inducible DNA repair systems of hyperthermophiles under high energy electron
bombardment. Analogous experiments will be conducted with selected marine Synechococcus
isolates. Subsequently, we will examine chromosome fragmentation and reassembly using
pulsed field gel electrophoresis, as in previous studies with the Co60 source.
An additional goal will be to understand the physiological responses of the radiation resistant
microorganisms to UV irradiation. We plan to utilize whole genome microarrays, as established
for Halobacterium sp. NRC-1 (Section 4.2.4.1) as well as CPD assays. A variety of tools, such
as antisera for CPD assays are available through our collaborator, Shirley McCready, at Oxford
Brookes University. Our goal is to determine the critical response of a diversity of UV-tolerant
cells to DNA damage via UV radiation.
4.3.2 Panspermia in young systems
Interstellar transport of material is extremely unlikely among field stars (Melosh, 2003), but
transfer rates among young stars in open clusters (the birth place of most stars) is orders-ofmagnitude higher (Adams & Spergel 2005). If transfer is possible (and likely), then the
implications for our understanding of the spread and origin of life is profound: life could
originate in only a handful of systems, yet the existence of life could be ubiquitous.
Are young solar systems fertile environments for the propagation and growth of life? We will
address this question by carrying out a series of linked investigations spanning from astronomy
to biology:
Confidential
Page 42
6/20/2017

How long can life survive in an open cluster? Basing our analysis on various sites of ongoing star-formation (e.g., Orion, Taurus-Auriga), the Princeton group will model the
ultraviolet and cosmic ray environments in open clusters. These variables will serve as
input for the experimental work outlined in the following section, where extremophiles
will be exposed to high radiation environments to determine their survivability.
 Do pre-biotic molecules survive the planet formation process? There has been
significant interest in the detection of pre-biotic molecules in molecular clouds (see
Sanford, 2005). Will complex molecules survive the proto-stellar environment? Where
in the early solar system can they survive? What is the rate of cometary delivery of these
molecules? 
 What is the transfer rate between planets during the early and late bombardment phases?
If life originated on Mars, what planets and moons could be seeded with Martian life?
What if life originated on Europa? What are the transfer rates between environments and
other solar systems in an open cluster? How does the dynamic focusing effects of Jupiter
alter these rates? We will extend the numerical analysis of Adams & Spergel (2005) to
these systems. 
 When do planets become habitable and what is the range of habitable environments? In
our early solar system, Mars was likely a far more hospitable environment: warmer and
with higher atmospheric pressure. On the other hand, most forms of life could not
survive in the early Earth. We will synthesize our panspermia studies with results from
relevant investigations by NAI teams (notably Woods Hole, Penn State, Arizona and
Colorado) to determine the optimal epochs and locations for cross-fertilization..
The standard view of the origin of life suggests that life originated on the early Earth very shortly
after it became sufficiently cool. If so, panspermia in young clusters could lead to life forming
spontaneously and independently on each habitable planet in a solar system. If the transfer rate of
life within the solar system is high enough, our solar system studies may find life forms on other
bodies that are closely related to terrestrial life, as discussed further in Section 4.4.2.
4.4 Test cases in the Solar System
4.4.1 Species survival on Mars
The survival of endemic and/or transplanted microbial life in the near-surface environment of
Mars must involve the interaction between microbial community and the sources of carbon,
nutrients, and energy in the Martian regolith and fractured bedrock. The COMB team will test
adaptation of terrestrial extermophiles to conditions that simulate various aspects of the Martian
regolith and bedrock. In previous sections of this proposal, we discussed exploring the cold
temperature limits for a variety of psychrophiles. These experiments will also involve control of
headspace gas compositions, in some cases to reflect interactions between the Mars atmosphere
and microenvironments in the Mars subsurface. In this section, we discuss appropriate liquid
media and solid phases to use for testing species survival and adaptation to Mars-like conditions.
A widely used simulant material for the Mars surface environment is the JSC Mars-1, which
consists of poorly crystalline volcanic ash of basaltic composition from the Pu’u Nene cinder
cone, Hawai’i (Allen et al., 1997). This ash source was chosen as a Mars regolith simulant
because of the similarity in the visible and near infrared the reflectance spectra between the
simulant and high emissivity (i.e., “bright”) regions of the Mars surface. However, since the
Confidential
Page 43
6/20/2017
Martian regolith has been exposed to weathering processes for potentially hundreds of millions
of years or longer, such chemical alteration would push this regolith to a composition that is
chemically and mineralogically more diverse than unaltered basalt. A broad cross-section of the
Mars research community has recognized that a single simulant is insufficient to characterize the
wide range of inhabitable chemical environments that may have existed and/or that may yet
persist on Mars.
In microbiology and astrobiology, a liquid medium derived from “rinsing” or overnight soaking
of the JSC Mars-1 simulant in DI water has been used. Modeled Mars brine chemistries could
also be used for enrichment cultures. Several magma derived minerals and their weathering
products have been detected or are inferred to exist on the Martian surface (e.g., zeolites,
smectites), though we are not aware of their use in psychrophile culturing work.
Another set of studies uses Mars Analog Environments on Earth as a starting point for assessing
natural microbial communities and preparing enrichment cultures, sometimes in media based on
the particular analogue environment sampled. Three such Mars analogue sites will provide our
group with field enrichment cultures for various psychrophile metabolisms. We plan to utilize
samples from cold environments investigated by our CIW collaborators, Andrew Steele and
Marlyn Fogel, through the NAI-funded Arctic Mars Analogue Svalbard Expedition (AMASE).
These environments possess a range of basaltic lithologies and include the outflows of
geothermal fluids that rapidly chill and provide disequilibrium chemistries in support many
different metabolisms. This work will be conducted through our collaboration with Steele and
Fogel, who have already provided F. Chen at COMB with sample material for enriching
psycrhophilic endolithic cyanobacteria. We will obtain further samples for halophile,
methanogen, and cyanobacteria cultures from Antarctica, via our collaboration with R.
Cavicchioloi, UNSW. We will also collaborate with TC Onstott, Princeton, to obtain samples
from the deep permafrost Mars Analogue site being developed in the Nunevat Territory, Canada,
by the Indiana-Princeton-Tennessee Astrobiology Institute (IPTAI). This site will be cored to a
depth of 0.5 to 1.1km to recover deep permafrost and cold brine samples.
In addition to chemical composition, the availability of water will have a mitigating influence on
the presence of viable life. A prior report tested the ability of three mesophilic (25-55 oC),
hydrogen utilizing methanogens to grow in a buffer containing a Mars soil simulant comprised of
altered volcanic ash containing different water concentrations (Kral et al, 2004). Results showed
that these species produced methane in the simulant under a carbon dioxide-hydrogen (95:5)
headspace. All three also generated methane in simulant without standing liquid, although the
effect varied with species. This study measured methanogenesis but did not confirm growth of
the methanogens.
Martian soil experiment
Specific aims: (1) To measure metabolic activities and growth of representative archaeal strains
under Mars-like conditions. The main emphasis will be on ionic composition of the medium,
mineralogic and chemical composition of the regolith simulant, and water content of the regolith
itself. (2) Establish enrichments for novel Bacteria and Archaea that thrive at high osmotic
strength of the medium and low to circumneutral pH.
Confidential
Page 44
6/20/2017
Experimental plans: Experiments will include tests for growth and metabolic activities of test
microorganisms in simulants from different locations and using a range of water content. We
will employ both traditional soil and soil water simulants (e.g., JSC Mars-1 and DI rinsate) and
simulants that explore other proposed habitable environments in the Mars subsurface (NaCl
brines and CaCl2 brines reacted with JSC Mars-1; ferric sulfate-rich brines at low pH; media at
low to circumneutral pH in contact with individual constituent minerals of basalts as well as
smectites and zeolites. In addition, Mars’ surface area contains high levels of iron oxides, which
could serve as an electron acceptor for dissimilatory iron reducing prokaryotes. Therefore, we
will also establish multiple enrichments to test for psychrophilic methanogens that can grow in
water containing only selected simulants and hydrogen/carbon dioxide and dissimilatory iron
reducting halophilic Archaea. Sediments from Arctic, Antarctic and permanently cold marine
sediments will be used as inoculum for cold adapted methanogens and cold halophilic sources
(e.g., Deep Lake) will be used as inoculum for halophiles. Methanogens will be monitored by
measuring methane production (metabolic activity) and by competetive PCR of 16S rRNA
(growth). Halophile growth will be monitored by plating. Once stable enrichments are obtained,
isolates will be obtained by standard dilution methods carried our either under oxic or anoxic
conditions. Isolates will be characterized by classical- and molecular microbiological methods.
The inocula for these enrichment cultures will derive from sampling in association with our
collaborators: Svalbard samples with Andrew Steele and Marilyn Fogel and the AMASE team,
Antarctic samples with R. Cavicchioli, and Nunnevat Territory, Canada, deep permafrost and
brines with TC Onstott and IPTAI.
4.4.2 Terrestrial contamination of the Solar System
Just as Martian material reaches Earth, large impacts distribute terrestrial material through the
Solar System. Nearly 5% of rocks ejected from Earth eventually reach the surface of Mars and
~1% reach the Jovian system (Melosh, 2003). These ejecta may transport terrestrial microbes,
and therefore have the potential to contaminate (and even seed) Mars or Europa, the two prime
astrobiological targets within the Solar System. Previous work has characterized ejecta number
and mass, and survival of a limited number of microbial organisms in transit; the experiments
outlined in Section 4.3.1 will expand our knowledge on the latter question. No study, however,
has examined what fraction of Earth-derived meteoroids impact the Martian and Europan
surfaces in a way that permits microbial survival. We consider that issue here.
Mars
Impact survival: The low-density Martian atmosphere acts as a filter to meteoroids: some ablate
completely; large meteoroids airburst; and still larger ones experience little deceleration and
strike the surface at hypervelocities (Chyba, 1993; Chyba et al., 1993; Mileikowsky, 2000;
Melosh, 2003). It is likely that this substantially reduces the total mass of viable microorganisms
that reach the Martian surface—though that number will certainly not be reduced to zero. We
propose to carry out a quantitative analysis, with the ultimate goal of establishing a lower limit
for background levels of natural biological contamination on the surface of Mars.
Our work will build upon numerical codes for the Earth’s atmosphere used to examine incoming
bolides of varying densities and sizes (Chyba, 1993; Chyba et al., 1993). Recent data from the
Mars Global Surveyor, Climate Orbiter, and Exploration Rover missions will be used to provide
input data for the atmospheric profiles. A range of bolide atmospheric entry incident angles will
Confidential
Page 45
6/20/2017
also be examined and the resulting aerobraking and ablation will be incorporated to estimate the
temperature evolution during entry through the Martian atmosphere. Thus, for a given mass,
density, entry angle, entry velocity, and microbial contamination level, our code will be able to
predict the fraction of viable microbes delivered to the Martian surface. This work will be
supervised by Chyba and pursued by his graduate student, Mr. Kevin Hand. This analysis will
not only enhance our understanding of microbial transfer between planets but will also prove of
interest for analyses of what level of “hitchhiking” microbes are permissible for spacecraft
traveling from Earth to Mars.
Europa
Europa is the premier place in our solar system to search for a separate origin of life. The lack of
atmosphere, added distance, and radiation of the Jovian system may well protect the sub-surface
liquid water environment from contamination by inhabited Earth-rocks. As a result, if we want to
learn the most about the diversity of life, Europa is the place to explore. Here we propose to
calculate the background biological contamination level experienced by Europa over its history.
Several stages leading to contamination must be considered: (1) delivery of a contaminated Earth
rock to the Jovian system, (2) delivery to the surface of Europa, (3) retention of material vs. loss
during impact (Pierazzo and Chyba, 2000), (4) delivery of material directly to the putative subsurface ocean via complete penetration of the ice shell (Turtle & Pierazzo, 2001), and (5)
survival time of viable biological material on or near the surface as compared to the shell
resurfacing timescale. For some stages, useful work has been published. However, many need
more complete analysis and a synthesis of all stages has never been attempted. We (CoI Chyba
and Hand) will build on previous simulations of meteoritic transfer to Europa to provide a
quantitative analysis of natural background contamination for both the surface and sub-surface of
Europa.
4.5 Summary
The principal tasks described in this section are as follows:
 We will use comparative experiments to study the adaptation to cold environments of
representative extremophile species.
 We will investigate mechanisms for cold adaptation using microarrays, gene transfer and
proteomics, and search for cross-species similarities.
 We will examine the radiation sensitivity of key extremophiles, and determine their
survival potential in the high-UV radiation environments on Mars-like planets and in
young star clusters.
 We will examine the growth potential of key terrestrial extremophiles in simulated
Martian environments.
 We will investigate the potential for terrestrial contamination of Mars and Europa.
Confidential
Page 46
6/20/2017
5. How will we recognize if life is present?
5.1 Introduction
Goal #7 of the Astrobiology Roadmap centers on identifying and recognizing signatures of life.
This is also a key aspect of NASA’s Vision for Space Exploration. Within the Solar System, the
capacity to sense life remotely would allow surveys of local environments, such as the surface of
Mars and the surfaces of astrobiologically interesting Solar System moons, even with ground
based instrumentation (Sparks, Hough & Bergeron, 2005). The next logical step, given such a
capability, is to include appropriate instrumentation as a payload element in spacecraft Solar
System exploration, such as Mars orbiters and the Jupiter Icy Moons missions. In this way, vast
areas of planet and moon surfaces can be surveyed for direct detection of life, and it is
straightforward to imagine such a procedure being a fundamental part of site selection for in situ
experiments made with landers and field instrumentation. Beyond the Solar System, remote
sensing is an absolute necessity and NASA is developing TPF to seek direct images and spectra
of Earth-like planets.
Currently our best remote sensing “biomarkers” are based on the ability of life to modify
atmospheric composition (Woolf & Angel 1998). NAI researchers (notably at NASA Ames and
CIW) have devoted considerable effort to exploring potential spectroscopic biosignatures, such
as O2 and the red edge due to chlorophyll absorption (Seager et al, 2005). These features can be
examined most easily in a planetary context through observations of the Earth itself, and we
describe a program of Earthshine observations later in this section. However, the interpretation
of spectroscopic biosignatures, and consequent inferences on the presence of life, are
substantially model dependent. It is important that we begin to build a more sophisticated
understanding of the likely many ambiguities that can arise. If an unambiguous biomarker exists,
it is vital that we know it! Circular polarization is such a candidate.
5.2 Validating circular polarization as a biosignature in terrestrial
environments
Circular polarization has been discussed in the astronomical literature for over 30 years as a
potential biosignature (Pospergelis, 1969; Wolstencroft, 1974). Over the last 18 months, we
(CoIs Sparks, DasSarma and Chen, and consultant Germer) have been investigating circular
polarization’s potential as a biosignature. Our initial laboratory experiments suggest that circular
polarization of light scattering from biological material can be orders of magnitude larger than
from abiotic material; this is consistent with prior work. We have begun astronomical application
of this technique, with imaging polarization of the Martian surface during the favorable 2003
opposition (Sparks et al, 2005). But the most effective means of proving circular polarization as
a biosignature is through measurements of targets that unequivocally include living material; that
is, measurements of environments here on Earth, in tandem with control abiotic samples.
We will verify the effectiveness of circular polarization as a biosignature through
1. Measurements of key natural substances in the laboratory
2. Measurements of terrestrial scenes in the field.
As part of this NAI proposal, we will construct a circular polarimeter instrument designed
specifically for in situ terrestrial measurements. If we can demonstrate that circular polarization
Confidential
Page 47
6/20/2017
works as a life detector on Earth, then application to future space missions, both interplanetary
probes and exoplanet observatories, is potentially straightforward yet incredibly important.
5.2.1 Circular polarization and life
Chirality of living matter
Life is predominantly homochiral. Almost all organisms use only left-handed or L-amino acids
and right-handed or D-sugars. This is likely a necessity for self-replication and hence will
characterize all biochemical life including currently unknown forms. Intriguingly, analysis of the
Murchison meteorite has shown L-excesses of 2-9% for a number of -methyl amino acids
(Cronin & Pizzarello 1997), with slightly smaller excesses found in the Murray meteorite
(Pizzarello & Cronin 2000). The origin of this phenomenon is not known; it could be an
amplified relic of an ancient excess, such as might be found in the interstellar medium or
prebiotic matter delivered to Earth, or it may arise from a surface interaction that favors one
handedness over the other. Nevertheless, it is a “universal” quality of terrestrial life, and chirality
studies have been proposed as a robust means of searching for life that may indeed differ from
terrestrial life, except in this regard.
Living matter can induce circular polarization
Organic material can be optically active, arising from differential absorption or scattering of left
and right circularly polarized light by its component chiral molecules (Pospergelis 1969,
Wolstencroft 1974, Winebrenner & Ashner 2000, Wolstencroft et al. 2002). Circular dichroism
spectroscopy is a standard analysis technique for studying protein structure (Purdie & Brittain
1993, Kelly & Price 2000). Chlorophyll, for example, induces 0.1- 1% circular dichroism in its
absorbance bands (Houssier & Sauer 1970). Circular Intensity Differential Scattering (CIDS)
considers the circular polarization induced by scattering from chiral samples and has been
studied by Bustamante et al. (1985). Circular polarization can also be caused by optical
interaction associated with the birefringence of macroscopic biopolymer membranes of an
organic structure, while light emitted through fluorescent processes, commonly associated with
molecules of biological significance, may also exhibit circular polarization. Microbes typically
produce fractional polarizations ~10-2 to 10-3 (Salzman & Gregg 1984). Here, we are interested
in the possibility that interaction between light and living organisms can produce a potentially
detectable signal ~0.1% or more, in the form of induced circular polarization.
Abiotic contributions to circular polarization arise from atmospheric and aerosol scattering and
from mineralogical scattering processes. While some minerals and crystals are certainly optically
active, integrating over a naturally produced sample is expected to give equal fractions of
enantiomorphs that average to zero in their optical activity. Multiple reflection and phase effects
can also introduce circular polarization; however, those would produce a smooth distribution
recognizably related to the geometry of the scattering (Bandermann et al. 1972). Empirically,
Solar System measurements of circular polarization consistently find very low fractional
polarization levels, ~10-4 to 10 –5 (Kemp et al. 1971, Swedlund et al. 1973, Meierhenrich et al.
2002), some two or three orders of magnitude smaller than likely organic effects. Even if local
enantiomeric excesses of optically active minerals were to exist, it is expected that they would be
distinguishable from circular polarization having an organic origin by their spectral properties
(Pospergelis 1969, Kelly & Price 2000). Fig. 1 shows the absorption and circular dichroism
Confidential
Page 48
6/20/2017
spectra of the chromophores of two primary microbial targets. Not only is there a substantial
circular dichroism signal but also there is a distinctive sign-flip through the absorbance bands.
It should be emphasized that biological signals are potentially two to three orders of magnitude
higher than abiotic signals and that polarimetry is capable of very high sensitivity and accuracy.
Fractional polarizations of ~10-6 are already achieved in current astronomical polarimetry thus
circular polarization spectroscopy offers a high potential for producing an unambiguous
biomarker.
Figure 5.1. Left: Absorption spectrum of chlorophyll (red, solid) and circular dichroism
spectrum (blue, dashed) adapted from Houssier & Sauer (1970). Right: Absorption spectrum
(red, solid) and circular dichroism spectrum (blue, dashed) of bacteriorhodopsin (“purple
membrane”), adapted from Becher & Cassim (1977).
5.2.2 Astrobiologically Relevant Samples
Light Harvesting: vitally important, maximally observable
An especially promising type of interaction between light and living organisms is photosynthesis
(used broadly here to represent biological systems which “harvest” light). We begin our study by
determining the circular polarization properties of important photosynthetic microbes.
 In photosynthesis, the organism extracts energy by triggering an electronic excitation of
the photosynthetic pigment in diffuse absorbance bands. Circular dichroism and related
phenomena can be induced from the intrinsic chirality of the molecules, from excitonic
coupling between chromophores in molecular complexes and from macroscopic
organization of the system (Garab 1996).
 From an astronomical perspective, photosynthetic activity is extremely attractive, since it
is largely a surface phenomenon, which is required for external optical observation. Apart
from the possibility of photosynthesis at ocean-floor hydrothermal vents, it requires
interaction with light of the host star (including the Sun!), hence is tuned to the
wavelength of maximum flux of the star and is tuned to the wavelength of maximum
transmission of the planetary atmosphere (Wolstencroft & Raven 2002).
These qualities combine to maximize the chances of detection through remote sensing.
Photosynthesis is not limited to vegetation, but also occurs in primitive microbial life. We have
therefore selected two of the most astrobiologically relevant microbial species for the first phase
of analysis:
Confidential
Page 49
6/20/2017
Cyanobacteria are photosynthetic prokaryotes possessing chlorophyll a and their unique pigment
phycocyanin. They have an extensive fossil record that may date back 3.5 billion years (Whitton
and Potts 2000). With the early development of a photosynthetic capability, they are widely
thought to have been the primary agent responsible for the rise of oxygen in the primitive
atmosphere on Earth. Cyanobacteria are abundant and ubiquitous in marine and freshwater
systems; are found in cold and hot environments; tolerate conditions of high desiccation; and can
produce biochemical products that provide protection from UV damage. High diversity and great
adaptability have made cyanobacteria extremely competitive on the planet Earth. These species
have been studied extensively as part of exobiological research on the limits of life in the Solar
system (Wynn-Williams 2000) and are now being studied as an analogue for past (or perhaps
even present) life on Mars.
Haloarchaea are salt-loving extremophiles, robust against UV radiation and tolerant of
desiccation. They use a light harvesting system that contains the retinal-based chemical pigment
bacteriorhodopsin in their metabolism. The tolerance shown by haloarchaea for harsh cold, dry,
salty conditions make this species a candidate for life on Mars, should such exist at the present
time (Landis 2001). Another example of the retinal-based rhodopsin system, proteorhodopsin,
has been found in light-harvesting plankton (Beja et al. 2001).
Figure 5.2 UV-VIS absorption spectra of chlorophyll (green) and retinal (purple)-based pigments. The
complementarity of the spectra indicates that these pigments may have co-evolved (DasSarma, 2004;
Wickramasinghe, 1976). The orange spectrum is for carotenoids.
All known light harvesting systems require one of either chlorophyll or retinal-based pigments.
A hypothesis proposed is that these two systems co-evolved, suggested by the complementarity
of their spectra (see Figure 5.2). With their profound importance in astrobiology and potentially
high observability, these organisms represent excellent targets for an initial study. We will use
existing laboratory measurements where possible, and make new measurements where needed,
to fully characterize the optical characteristics of light scattered by such organisms.
Sample preparation and measurement strategies
Our team has in-depth expertise on all aspects of the primitive photosynthetic haloarchaea and
cyanobacterium organisms and permanent laboratory facilities dedicated to the culturing and
Confidential
Page 50
6/20/2017
study of the two proposed primary astrobiological targets, halobacteria (DasSarma) and
cyanobacteria (Chen). For new measurements, our strategy will be to culture the microbial
samples, which will be transported to NIST for polarization study.
5.2.3 Background and preparatory work
Pilot studies at NIST
(a)
(c)
(b)
Figure 5.3. Pilot study from NIST showing
circular polarization from 300 nm to 900 nm of
reflected light from (a) halobacteria (b) a maple
leaf (c) a control white mineral. Dark points
use a blue sensitive PMT and lighter points, a
red-sensitive PMT. The extra signal in the red
for (a) may arise from fluorescence. The control
sample upper limit is more than an order of
magnitude lower than the detections.
We have explored the measurement of circular polarization in the laboratory at NIST,
experimenting with leaves and haloarchaea. We used a high precision photoelastic modulator
(PEM), coupled with a monochromator and a photomultipler tube (PMT) detector, to measure
the circular polarization of reflected light; some example results are shown in Figure 5.3. In a
few sets of measurements, we found that blue and red sensitive PMTs gave inconsistent results
(e.g., Figure 5.3a). This is probably due to organic fluorescence, but emphasizes some of the
pitfalls of this type of measurement. In all cases, when we measured a control sample, we
obtained null polarization at a level of 0.001%, consistent with expectations. In contrast, the
biological samples gave polarizations a few tenths percent, at least an order of magnitude
difference. These preliminary results underline the value of pursuing these investigations.
Astronomical observations of Mars
We have started to extend these measurements to bodies in the Solar System: we obtained high
quality imaging circular polarimetry of the Martian surface during the extremely favorable
opposition of 2003, (Sparks et al. 2005). We used the European Southern Observatory Very
Large Telescope (VLT), one of the world’s forefront telescopes, in an imaging polarization mode
that achieved polarization noise levels slightly below 0.1% and spatial resolution 210 km, but did
not find any regions of circular polarization (Figure 5.4). This represents the best achievable
results for current imaging astronomical instrumentation, and demonstrates the feasibility of the
Confidential
Page 51
6/20/2017
technique. Our observations covered only a small fraction of parameter space, so although we
obtained a null result locally, we could not exclude the presence of optical activity at other
wavelengths, in other locations or at higher spatial resolution, nor life in the wider sense.
Figure 5.4 Observations of Mars. True color representation of VLT Mars observations (upper left) on
cylindrical projection, with (lower left) outline of their footprint overlaid on USGS/NASA processed Viking
image data. On the right, images of circular polarization degree (upper), displayed approximately 0.2% and
absolute value of polarization degree (lower), 0 to 0.2%. The low level structure demonstrates a noise floor
arising from internal interference within the CCD
5.2.5 Constructing a prototype field polarimeter
We propose to construct a transportable prototype circular polarization instrument to make in
situ measurements in a variety of terrestrial environments. The requirements to be determined for
instrumentation will cover the spectral resolution and precision of polarization measurement
needed in conjunction with the timescale on which measurements will be needed, and will be
determined from existing literature augmented by additional laboratory polarization
measurements which we will carry out at NIST during year 1. There will be trades between the
need for speed, spectral coverage and precision. Liquid crystal modulators provide a flexible
array of capabilities for beam referencing and switching, with timing capabilities well-matched
to CCD readout speeds. In a field or astronomical application, improved spatial resolution
improves discrimination of localized signals against the dilution of background mineralogical
scenery. Hence, for our baseline configuration we envisage coupling polarization modulators to a
robust, standard dual-beam imaging system with tunable wavelength. (We could, following the
design studies, choose to emphasize spectral coverage, but the hardware component
requirements would be very similar.)
With an understanding of the levels of polarization introduced by realistic analogues of
extraterrestrial life and with an understanding of the likely spectral characteristics, it will be
possible to optimize studies of this form and to design instrumentation dedicated to the task. We
propose to undertake a 3 month optical design study based on our derived requirements,
followed by assembly of proto-type hardware intended to be useable in the field. The
Confidential
Page 52
6/20/2017
requirements and design phase will last approximately one year. Construction and testing of the
instrumentation is expected to cover the second and third years, with enhancements and
application for years three and four. Results will be published in the refereed literature.
5.3 Specular reflection from oceans on exoplanets
Figure 5.5 Diurnal variations in reflectivity as the Earth’s terminator moves across its surface and
the geometric location of the specular reflection point for the Sun crosses coastlines.
We (CoI Turner working with Princeton graduate and undergraduate students) will further
Specular reflection from the Earth’s oceans produces a substantial fraction of its total brightness
and polarization; these can both change rapidly when the planet’s rotation causes the geometrical
position corresponding to specular reflection to cross a coastline, as indicated in Figures 5.4. An
analogous feature in the light curve of an exoplanet could provide an indicator of the presence of
large bodies of liquid on the planet’s surface (Ford, Seager & Turner 2001; Seager, Ford &
Turner 2002). In principle at least, a sufficiently extensive exoplanet light curve data set, ideally
including polarization information also, could be used to construct a map of major land/sea
divisions over some range of its latitudes (depending on the system viewing geometry)! The
existence of large bodies of liquid, perhaps inferentially identifiable as water based on
spectroscopic information, would obviously constitute a very important and provocative
biosignature.
We (CoI Turner working with Princeton graduate and undergraduate students) will further
investigate this speculative possibility with an emphasis on its practical limitations and potential
false positives. Using further simulations along the lines of those employed by Ford et al.
(2001), we will seek to understand issues such as the systematic noise introduced by variable
cloud cover, dilution of the specular reflection signal by other sources of polarization in the
planet’s scattered light (e.g., atmospheric Rayleigh scattering), requirements for signal-to-noise
Confidential
Page 53
6/20/2017
ratios, light curve durations and sampling rates in order to reliably identify an exoplanet
coastline, and so forth. A long-term goal would to develop automated techniques for coastline
detection and/or mapping that could be applied to simulated or actual light curves of the Earth as
a reference case for the method. The modeling work described here would also support attempts
to detect the effect via the Earthshine observations described the following section.
5.4 Spectral signatures of land, ocean, weather and life
At the present time Earth is the only planet known to harbor life, and studies of Earth are our
starting point in characterizing any remotely detectable biosignatures. How would Earth appear,
if seen from afar as an extrasolar planet? Could a mission like NASA's TPF determine if such a
planet were habitable, or even inhabited? To what extent could we hope to learn about
continents, weather patterns, and seasons? In this section, we propose tandem field projects
focused on seeing Earth as a planet and exploring the dry limits of habitability in our own world.
5.4.1 Earth as an “Exoplanet”: Earthshine Over Time and Space
Earthshine, light from the dayside Earth that can often be seen illuminating the dark side of the
crescent Moon, contains the globally integrated spectrum of the sunlit Earth as it would be seen
from afar as an extrasolar planet. In fact, Earthshine observation is currently the most accurate,
inexpensive and easy way we have to monitor the spatially integrated spectrum of our own
planet. While satellite spectra can provide useful information for small areas, summing such
spectra does not accurately characterize the planet as a whole. In particular, the atmospheric
path-length increases toward the limb, whereas satellites peer directly downward. Furthermore,
satellite spectra are not taken with the full range of solar illumination angles, and large areas can
only be captured over long time-periods. All of this dilutes changes we might expect to see due
to weather and seasons.
In previous work carried out separately by CoIs Turnbull, Turner and their respective
collaborators (Seager et al. 2005; Woolf et al. 2002, Turnbull et al., 2005), Earthshine data were
used to characterize the atmospheric, surface and biological signatures we might look for in an
extrasolar planet spectrum. Rayleigh scattering, ozone and oxygen absorption, and atmospheric
water vapor are the strongest signatures, and all are strongly suggestive of habitability; oxygen is
suggestive of life itself. In some studies (e.g. Woolf et al, 2002, Tinetti et al, 2005), the spectral
signature of vegetation was apparently present, but individual surface signatures are difficult to
extract because the spectrum is spatially integrated over the entire planet.
In order for distinct surface signals to emerge, we need to obtain spectra from many viewing
angles of the planet. Spectrum-to-spectrum changes can be extracted at a higher level of
significance then can unknown signals from a single spectrum. A major limitation of Earthshine
studies conducted so far is that only two views of the planet are possible from a single observing
site. The morning crescent Moon sees Earth to the east of our telescope, and the evening crescent
Moon sees a view of Earth to the west (Seager et al. 2005). In order to capture other views of
Earth, we must observe the Earthshine from other longitudes.
Characterizing the Earth’s spectrum from many angles allows us to address two areas of
astrobiological inquiry. First, how significantly would the spectrum of an Earth-like planet
change as it rotates? This variation with longitude allows us to extract spatial information from
Confidential
Page 54
6/20/2017
a spatially unresolved signal. Second, how does the spectral signal change, as the view of Earth
evolves from continent to ocean, from forest to desert, and from season to season? Or are these
changes “masked” by the ever-changing landscape of clouds? Observing Earthshine over an
extended time period will allow us to realistically assess the visibility of such signals for
extrasolar planet discovery missions.
To answer these questions, we propose a Portable Earthshine Monitoring System (PEMS).
The essence of this project is simple: by transporting a small telescope and spectrometer to
appropriate observing locations, we can spectrally characterize the entire planet in both space
and time.
Before describing this system in detail, we address an obvious question: Why use a portable
system, instead of simply obtaining telescope time at various observatories around the world?
Briefly, the reasons that a portable system is preferable are as follows:
1. For extended-source observations (such as the Moon), the important factor in obtaining high
signal-to-noise is large beam size (i.e. low spatial resolution). Thus large (>1-m) telescopes
(with high resolution and small beam size) are neither necessary nor desirable for Earthshine
observations,
2. Small telescope (<1-m) observatories are not typically equipped with medium- to highresolution spectrographs as would be required for this program,
3. The quality of Earthshine data are strongly affected by slit-length and thus a single custommade instrument is needed (explained below),
4. Adapting a single, portable instrument to the diverse “back-end” interfaces of a suite of
existing large telescopes would be time-consuming, awkward and expensive,
5. Local weather-patterns, which strongly affect data quality, can be avoided with a portable
observing system, and
6. This project can be combined with the “Earth before Trees” project, thus achieving greater
scientific return for essentially the same investment of travel time/costs and other resources.
Turner and Wyithe have already acquired a portable Earthshine observing system that is based
on commercially available equipment, specifically a Meade 203 mm LX200GPS-SMT SchmidtCassegrain telescope equipped with a Pictor 1616XTE CCD imaging camera and autoguider plus
f/3.3 and f/6.3 focal reducers and a standard BGR filter wheel. The unusually large dynamic
range of this camera and the use of neutral density filters permit us to obtain photometrically
useful images of both the sunlit and Earthshine illuminated portions of the lunar surface with
(separate) exposures of reasonable length. This system will be employed initially to search for
the abrupt Earthshine brightness variability expected to be associated with coastline crossings of
the Sun to Moon specular reflection point on the Earth’s surface (see Section 5.3). First
observations will be obtained from the Mt. Arapilies area, west of Melbourne, Australia. For
more general purpose Earthshine observations, we will add a spectrograph to the instrumentation
Confidential
Page 55
6/20/2017
package, ideally a long-slit spectrograph which allows simultaneous collection of data from the
Earthshine illuminated portion of the lunar surface and the nearby background sky (in order to
measure and subtract the scattered light from the much brighter sunlit portion of the Moon’s
image). This may require the construction of a simple and portable special purpose spectrograph
since most commercially available units suitable for mounting on a small telescope are fiber-fed
and thus do not provide spatially distributed spectral data.
In November-December 2005, Turner and Turnbull will visit their collaborators in Australia (S.
Wyithe and his students at the University of Melbourne Physics Department) to test the existing
observing system, establish a standardized observing protocol, and plan a 5-year observing
campaign. The Earthshine observing experience of Turnbull, modeling experience of Turner, and
instrumentation experience of Wyithe give this program a high probability of success.
5.4.2 Earth Before Trees:
Globally-Integrated Spectra
Ground-Truth Spectroscopy and Synthetic
In tandem with the PEMS observing initiative (i.e., from the same field sites), we are beautifully
equipped to carry out field studies that complement our study of the Earth as an “extrasolar”
planet and as an “extreme” environment. In particular, there are two questions we can explore:
How would the Earth appear as a “microbial” planet, without the existence of higher plants, as it
was for the vast majority of its history? And how would the Earth appear as a “desert planet”, in
either the case of much colder, or much hotter climate conditions?
At the same time as our team is conducting Earthshine observations, we propose to spectrally
characterize the Earth as it would appear in the absence of trees by obtaining ground spectra of
(a) land areas that are dominated by microbial mats and (b) deserts. These spectra can be used
in conjunction with software produced by the NAI Virtual Planetary Laboratory to create 3-D
synthetic models of the desert Earth, and of the Earth without trees. These models will serve as
interesting comparisons to the spectra generated with our “ground truth” data.
Many of the un-vegetated sites that need to be visited for this program are also ideal sites for
astronomical observation, by virtue of the fact that they are not prone to frequent precipitation or
clouds. Thus, with one team traveling to selected field sites, we can efficiently carry out two
missions, with one portable spectrometer pointing upwards and another pointed down.
Again, before proceeding with the details of this project, we ask the obvious question: Why not
use satellite data for desert regions and regions dominated by microbial mats? While satellite
data can and will be a useful tool, those data are not sufficient for the following reasons:
1. Satellite data products are tailored to meet the needs of the satellite mission. Typically, this
means that “spectral” coverage is really broadband imaging, with passbands chosen to elucidate
specific phenomena (e.g., vegetation species, vegetation health, or minerals). Our goal is to
cover the entire optical and near-IR spectral range at moderate resolution (R~600), focusing on
different spectral signals generated by microbial and non-biological groundcover.
2. Satellite data products that are “hyperspectral” in nature (i.e., not broadband) are obtained at
extremely high spatial resolution, covering very small, select land areas, and of limited
Confidential
Page 56
6/20/2017
wavelength coverage. Our goal is to spectrally characterize sites that cover a range of latitudes
as well as the most extreme dry climate conditions, from the hottest to the coldest deserts on the
planet, and containing surface microbes that inhabit polar to equatorial regions.
3. Hyperspectral data are not widely available, and the extraction of a data product that can be
converted into a spatially integrated spectrum may require hiring special personnel to work with
mission databases. Our goal is to carry this project out efficiently and in a manner where the data
extraction procedures (and thus any artifacts) are clearly understood by the users.
We propose to choose our PEMS field sites to be dually interesting for field spectroscopy of
microbial groundcover and extreme desert conditions that are not expected to harbor extensive
biological communities. Once ground spectra have been obtained, these ground truth data will
be used, in conjunction with NAI VPL software, to create 3-D models of the “microbial Earth”
and the “desert Earth”. These models, in comparison with our Earthshine Observations, will
allow us to explore ways to spectrally distinguish between macrobiotic surface signatures,
microbiotic surface signatures, and surfaces with little or no biological ground cover.
The equipment required for this investigation is a simple field spectrometer, and for our purposes
an off the shelf Ocean Optics Model S2000 spectrometer plus small laptop are entirely sufficient.
Turnbull has prior experience with field spectrometers and will be the primary observer.
5.5 Solar-System bodies as exoplanet analogues
Perhaps the most challenging aspect of pursuing an astrobiologically motivated study of an
exoplanet will be extracting the relevant signal(s) out of the sparse flow of photons from its point
image and arriving at a compelling, or at least plausible, interpretation of any possible
biosignature(s). It seems likely that the endeavor will require a major research program involving
many investigators, as diverse a suite of data as can be obtained and, no doubt, an extensive and
controversy filled literature. In this context, it will surely be instructive and useful to prepare for
these coming investigations of exoplanets by studying the relatively easy cases of Solar System
bodies, for which something akin to “ground truth” information has been or eventually will be
obtained by space probes. A particularly significant dimension of the problem that can be
explored, in analog, via study of Solar System objects is the relation of spatially unresolved
biosignatures to the complex underlying spatially differentiated properties of a target body.
In this context, CoIs Turner and Chyba, working with Princeton graduate and undergraduate
students, will carry out two projects combining ground-based observations with modeling
studies. The former has extensive experience with optical observations with a wide variety of
telescopes, instrumentation and targets, and the latter is similarly expert in modeling of bio- and
geo- chemical processes on Solar System bodies. The ready availability of observing time on the
APO 3.5-meter and its remote observing and fast instrument change capabilities make the
proposed programs far more practical than it would be with more conventional large telescopes.
5.5.1 Martian methane
Great excitement and considerable controversy has attended the apparent spectroscopic detection
of methane in the Martian atmosphere at abundances far above expected equilibrium levels
(Mumma et al. 2003; Krasnopolsky, Maillard & Owen 2004; Formisano et al. 2004) and with a
Confidential
Page 57
6/20/2017
distribution that appears to be spatially and perhaps temporally variable. Whether the source of
this methane ultimately proves to be due to Martian life or abiogenic in nature, it seems sure to
provide us with a powerful, and potentially sobering, lesson re the issues that will arise in
interpreting biosignatures associated with exoplanets.
Using the NIC-FPS (Near Infrared Camera - Fabry Perot Spectrograph) instrument available on
the APO 3.5-meter telescope, we plan to monitor Martian methane via narrow band imaging
centered on NIR methane bands, and adjacent control bands. These data should allow us to
examine the spatial and temporal variability of any Martian methane signal over periods of
weeks to months. Previous work on Martian methane has used non-imaging spectrographs and
thus provides only limited information on the spatial distribution of the signal on the Martian
surface, although there are claims that it is significantly inhomogeneous (Formisiano et al, 2004).
If there are well localized, and potentially time variable, sources (venting) of the methane that
produces the relatively diffuse signal seen spectroscopically, it will be much more easily detected
via the high spatial resolution provided by the Fabry-Perot. Moreover, a synoptic monitoring
program with a modest cadence (e.g., bimonthly during periods close to opposition) will provide
a greatly increased chance of detecting episodic methane venting, whatever its source. And even
a null result will provide a constraint on possible origins of the Martian methane.
5.5.2 Europa & Enceladus: ice planet prototypes
If liquid water is a necessary condition for life, then ice-covered worlds with sub-surface oceans
may well be the dominant reservoir for life beyond Earth (e.g., Chyba, 1997, and refs. therein).
Within our own solar system, the volume of liquid water believed to exist below the ice and rock
shells of the Gallilean satellites is 20-35 times greater than all of the liquid water on Earth
(Spohn and Schubert, 2003). And one can add to this the recent, surprising, discovery by the
Cassini probe of possible cryovolcanic activity on Saturn’s moon Enceladus.
While icy worlds may be of direct importance for habitability, their spectral signatures may also
serve as false positives for inhabited planets detected by missions such as the Terrestrial Planet
Finder. Compounds typically associated with biological processes, such as O2 and O3, are known
to exist on the surfaces of the Galilean satellites (Spencer and Calvin, 2002; Johnson et al.,
2004), and yet we do not claim that these worlds are inhabited based on these spectroscopically
intriguing results. Instead, we know that the interaction of the Jovian magnetic field with the
surface ice results in radiolytic chemistry that produces a host of interesting oxidants. Such
chemistry has been observed via spacecraft observations, space telescopes and ground based
telescopes, and it is currently being studied in the laboratory environment (Vidal et al., 1997;
Carlson et al., 1999; Moore and Hudson, 2000; Cooper et al., 2001).
If missions aiming to discover habitable exoplanets are to be successful, they must be able to
differentiate between non-biological planetary processes that can produce chemical
disequilibrium (e.g. radiolysis or photolysis) and biological processes that are producing a true
spectroscopic signature of life on another world. (Such radiation-driven disequilibrium could, in
fact, be an important factor for the habitability of subsurface oceans on icy satellites (Chyba and
Hand, 2001), but the radiation chemistry will occur whether there is an ocean under the ice or
not.) Here we propose to address this issue through numerical models and ground based
telescopic observations of icy worlds in our solar system.
Confidential
Page 58
6/20/2017
Detecting water from optical spectroscopy
A reasonably thorough literature search reveals that very little modern, high resolution
spectroscopy of these two bright objects (by the standards of large telescopes and CCD
instruments) has been published and essentially none that explores a broad wavelength range, as
opposed to concentrating only on some specific, pre-selected feature. The APO 3.5-meter
Echelle yields a spectrum covering the entire CCD band (roughly 350-950 nm) at a resolution of
30-40 thousand in a single exposure; thus, the potential for serendipitous discovery via
exploration of new domain of data is substantial.
Using the currently available high dispersion echelle and later the NIR moderate dispersion
TripleSpec (to be available within approximately two years) spectrographs on the APO 3.5-meter
telescope, we will monitor these two moons to search for features indicative of their subsurface
liquid environments, such as time variable, narrow water absorption lines. Gas phase water vapor
escaping from such a moon’s surface should produce far narrower lines than surface water ice,
and those features are potentially stronger and more variable than absorption due to any thin
atmosphere produced by sputtering processes. The latter might also distinguish itself via
correlation with leading vs trailing hemispheres. If such spectral indications can be identified,
they will not only be useful for understanding the geologic activity on Europa and Enceladus on
a longer time scale and with a better sampling rate than can be achieved with space probes but
will, again, provide an example of what can be learned about such complicated objects based on
limited and spatially unresolved data, one that will perhaps be instructive for interpreting the
spectra of exoplanets.
Observing Europa: the oxygen problem
Understanding oxygen in Europa’s surface ice is important both to assessing the ways a variety
of worlds could show signs of oxygen under remote observation, and to understanding the types
and abundances of electron acceptors that could ultimately be available to support a possible
biosphere in Europa’s ocean (Chyba and Hand, 2001). Condensed (gamma) phase oxygen has
been proposed to explain the observation of the 5771 angstrom feature on the surface of Europa
(Spencer & Calvin, 2002), yet it is known that solid O2 is not stable at average Europan surface
temperatures (~100K, Johnson et al, 2005) and pressures ~10-6 µbar (Hall et al, 1995)).
Radiolytic production of O2 is expected as a source, but laboratory measurements of this process
have not fully clarified the process (Vidal et al, 1997; Moore and Hudson, 2000) and it seems
unlikely that radiolytic production could offset atmospheric and ionospheric loss. Thus the
observation of condensed oxygen presents a stability problem, requiring some form a trapping
mechanism to retain the oxygen in the surface ice.
Recently we proposed that the oxygen problem could be solved by invoking formation of a
mixed SO2-CO2-O2 clathrate (Hand et al., 2006). Clathrates of these compounds are poorly
studied and thus our identification of such material on other worlds is limited. Here we propose
to use a field-deployable, visible, near-infrared, and mid-infrared Fourier transform spectrometer
to study clathrates of CO2, SO2, CH4, O2, and N2. Raman spectroscopy and X-ray diffraction
studies have been used in the past (Champagnon et al., 1997; Kuhs et al., 1997), but in order to
remotely observe this ice phase with a spacecraft or space-telescope we must understand the
spectral behavior in the visible, near-infrared, and mid-infrared. This suite of instruments has
Confidential
Page 59
6/20/2017
been successfully used in the Dry Valleys of Antarctica for analysis of both microbiology and
mineralogy (Hand et al., 2005). We will bring this instrument to MBARI to study CH4, to the
Polar Ice Core lab in Denver, CO, to study N2, O2, and CO2, clathrates in ice cores, and to
Yellowstone Park during the winter in order to study naturally occurring SO2 clathrates around
the sulfur-rich hot springs.
Observing Europa: Radiation-Induced Planetary Fluorescence
As the Jovian magnetic field sweeps past Europa with its 11-hour synodic period, the radiation
impacting the trailing hemisphere of Europa will cause the surface ice to fluoresce in the UV and
visible parts of the spectrum. During normal observations of Europa this fluorescence is
overwhelmed by solar photons reflected from the surface. During an eclipse of Europa by
Jupiter, however, such fluorescence could be detected. The orbital geometry required between
the Earth, Jupiter, Europa, and the Sun makes such observations rare and difficult, but with
dedicated telescope time at the Apache Point Observatory we propose to observe this effect over
the course of the next five years. During December of 2005, nine such eclipses of Europa occur,
but only four occur at night and all of the eclipses merge with occultations by Jupiter. The best
opportunities during 2005 were in January and February, when the trailing hemisphere was
visible during eclipse events that started far away from the disk of Jupiter (all ended with
occultations, however) (Gillard and Holdaway, 2003). Thus, while the ideal conditions are rare,
they are frequent enough to warrant a concerted effort to observe this phenomenon.
The detection of this radiation-induced planetary fluorescence is important both for our
understanding of processes that are occurring on Europa and for understanding possible radiation
induced signatures from exoplanets. On Europa, radiolytic chemistry resulting from high-energy
electron bombardment is believed to be occurring primarily on the trailing hemisphere.
Understanding the global distribution of the radiolytic chemistry will aid us as we work to
understand the role of exogenous and endogenous compounds on the surface of Europa and the
consequences of such chemistry for life in the proposed sub-surface ocean (Paranicas et al.,
2001; Carlson et al., 2005). Beyond our solar system, stellar and planetary magnetic fields will
likely play a significant role in planetary surface and atmospheric chemistry; thus, understanding
the signature of magnetically-trapped radiation on a moon in our Solar System may provide a
useful context for future exoplanet studies.
5.6 Summary
The principal tasks described in this section are as follows:
 We will validate circular polarimetry as a remote signature of life through measurements
of terrestrial organisms and environments.
 We will use Earthshine observations to obtain an accurate characterization of potential
spectroscopic biosignatures.
 We will acquire and analyze astronomical observations of Mars and the icy satellites,
Europa and Enceladus, testing potential methods of exoplanet classification.
Confidential
Page 60
6/20/2017
6. Management Plan
6.1 Structure and organization
Our project has a relatively simple management structure, illustrated in Figure 6.1. The PI, Neill
Reid, has overall responsibility for the project. He will serve as the point of contact with external
groups, including our Science Advisory Council, and the NASA Astrobiology Institute.
NAI
STScI Management
Community Missions Office
Executive Council
PI (Reid)
Institutional PIs
(DasSarma, Turner)
Program Manager
SAC
Science Advisory Council
Exoplanet
detection
Exoplanet
properties
SNH
Census
Life and
Cold
Life and
Radiation
Mars &
Europa
New
Biosignatures
Kasdin
Littman
Vanderbei
Paczynski
Valenti
Livio
Turner
Tremaine
Sowers
Robb
Valenti
Reid
Turnbull
Soderblom
Postman
Nota
Paczynski
DasSarma
Sowers
Robb
Reid
Chyba
Robb
DasSarma
Chen
Spergel
Turnbull
Chyba
Turner
Sparks
Sowers
Turner
DasSarma
Chen
Turnbull
Sparks
Figure 7.1 The management structure of the NAI proposal team. The investigators are color-coded by
institution: red, for STScI; green for COMB; and blue for Princeton.
Our program involves three major partners: the lead institution STScI with the PI, 7 CoIs, and 4
collaborators; the Center of Marine Biotechnology (COMB), with 4 CoIs, 2 collaborators and
two graduate students; and Princeton University, with 8 CoIs, 1 collaborator and four graduate
students. Both STScI and COMB are located in Baltimore. Our project involves additional
collaborators from a further 11 institutions, including Morgan State University, the University of
New South Wales, Idaho State University, the Carnegie Institution of Washington, the
University of Tokyo, the National Astronomical Observatory of Japan, the University of
Melbourne, the Smithsonian Astrophysical Observatory, NIST, the Jet Propulsion Laboratory
and Oxford Brookes University. Our collaborators include members of three other NAI teams
(Carnegie, JPL/Caltech and Indiana). Prof. S. DasSarma will be the institutional PI for COMB,
co-coordinating research, including contributions from collaborators, and serving as the main
point of administrative contact, and Prof. E. Turner will perform the same functions for
Princeton.
The large number of investigators and institutions requires that considerable attention be paid to
ensuring a true team effort that is both productive and cost effective for the scientific community
Confidential
Page 61
6/20/2017
and NASA. We have included specific labor in the proposal to provide oversight and
coordination, which will take the form of a senior Program Manager at STScI. There are several
highly qualified STScI managers with extensive experience in coordinating large and
complicated efforts such as HST servicing missions, and large hardware and software
development projects (our recently released Astronomer's Proposal Tool is a specific example).
The Program Manager will be devoted to the NAI program at 0.3 FTE per year. S/he will work
closely with the PI to establish the schedule (including milestones) and co-ordinate with COMB
and Princeton University work and deliverables. The Program Manager will also ensure rapid
disbursement of funds to the subcontracted institutions, Princeton and COMB.
The PI and the two institutional PIs, together with the Program Manager, will form the Executive
Council of this project. They will collectively decide outstanding issues involving intellectual
property rights and publication policy, and they will adjudicate should any disputes arise. Should
the Executive Council fail to reach agreement, the final decision rests with the PI.
Coordination within the science team requires good communications. All three institutions have
existing telecon and video conferencing facilities that will be used for this purpose. In addition,
STScI has pioneered the technique of web casting and archiving, which will be utilized to
provide widespread access to and permanent records of our activities. We have a great deal of
experience working as part of a large and widely dispersed team to tackle challenging projects.
We anticipate regular (weekly) internal meetings at STScI, COMB and Princeton to review
progress. We will hold bi-weekly team videocons to review overall progress and enable good
communication and cross-disciplinary discussion among the broader team.
We will organize a Workshop on Astrobiology and Extremophiles in years 1, 3 and 5; COMB
faculty and staff will take the lead in planning and execution. In the intervening years we plan to
have 3 to 4-day retreats for the full team, to foster interaction between different research projects.
At STScI, the NAI project will fit seamlessly into the existing management structure as part of
the Community Missions Office (CMO), which currently oversees several externally funded
projects that are independent of our prime contracts for the Science Operation Centers to operate
the HST and JWST, including MAST ($1M), and STScI’s involvement in the Kepler Discovery
mission ($7.5M) and the National Virtual Observatory ($2M). The internal STScI management
oversight would include a monthly CMO status meeting, a written monthly summary of project
status, and a quarterly oral presentation on the status of the project to STScI’s senior
management team. Existing administrative and management personnel are already in place to
support this project. This provides our team with unique management assets that are efficiently
leveraged from a large existing experience base.
It is our view that a project of this scope will also benefit greatly from a completely independent
external Science Advisory Council (SAC) convened annually to provide a thorough review of,
first, the plan, and then the progress of the project. This group will be a team of 5 astronomers,
planetary scientists, geologists or biologists, selected by the Executive Council, who will provide
advice on on-going activities and on relative priorities within the project. We anticipate that
individual members of the SAC will serve 2-3 years.
Confidential
Page 62
6/20/2017
6.2 Schedule: Milestones and deliverables
Table 6.1 shows the major milestones and deliverables for the main research projects that are
part of our NAI proposal.
SNH Census HighCold life Radiation Exoplanet
New
contrast
& life
properties
biosignatures
imaging
Construct v1.0 Complete
Initiate cold
Initiate
M- Design
field
Year 1
database
and
populate
with
stellar data
Year 2
Release
v1.0
SNHC to team
members
for
additional input
Implement
initial
habitability
criteria
Identify
and
acquire
key
missing data
Year 3
Update
and
expand
SNH
Census to v2.0
Release to NAI
community for
additional input.
Incorporate
results
from
dynamical
models
Continue
to
acquire
key
missing data
Update
SNH
Census
with
initial
results
from Kepler
Update
habitability
criteria
Year 4
Year 5
Release v3.0 of
SNH Census
Confidential
TPF-C mask
designs
Design Subaru
masks
Begin analysis
of
speckle
algorithms
Complete
analysis
of
close-loop
amplitude
control
Manufacture
Subaru pupil
masks
Begin optical
design
if
HiCIAO
coronagraph
module
Deliver pupil
designs
for
ground-based
instruments
Propose
for
funding
for
HiCIAO
coronagraph
& salt, cold
& anoxia,
and
cold
and
CO
experiments
dwarf
flare
monitoring in
open clusters
Initiate Phase
II
planetary
system models
Continue
cold & salt,
cold
&
anoxia, and
cold
and
CO
experiments
Initiate
biofilm and
gene
transfer
studies
Continue Mdwarf
flare
monitoring
Initiate
Mdwarf
ageactivity
program
Continue
Phase
II
planetary
system
modeling
Continue
low temp.
growth,
biofilm and
gene
transfer
studies
Initiate
ecological
and
proteomics
studies
Initiate
extremophile
radiation
sensitivity
experiments
Initiate
Martian soil
experiments
Manufacture
and
deliver
pupil
plane
coronagraph
for HiCIAO
Complete
biofilm and
gene
transfer
studies
Complete
ecological/
proteomics
studies
Continue
extremophile
radiation
sensitivity &
Mars
soil
experiments
Ground-based
observations of
SNHC targets
with HiCIAO
Complete
radiation
sensitivity &
Mars
soil
experiments
Page 63
Continue Mdwarf
flare
monitoring in
open clusters
Continue ageactivity
program
Complete
Phase
II
planetary
system models
Initiate `small
planet’ models
Complete Mdwarf
flare
monitoring
Complete ageactivity
program
Continue
`small planets’
modeling
Complete
`small planets’
program
circular
polarimeter
Construct
traveling
Earthshine
system and make
first observations
Start
construction of
field polarimeter
Continue
Earthshine
measurements
Complete
construction of
field polarimeter,
start laboratory
test
measurements
Continue
Earthshine
measurements
In
situ
measurements
with
field
polarimeter
Complete
Earthshine
measurements
Complete field
measurements
with polarimeter
6/20/2017
6.3 Key personnel
Dr. I. N. Reid (STScI) is the Principal Investigator. He has overall responsibility for the project
and will serve as the point of contact with external groups. He has conducted research on nearby
low-mass stars and brown dwarfs for over two decades, and has recently applied his expertise in
Galactic structure to investigations of the properties of exoplanet host stars.
Prof. S. DasSarma (COMB) is the institutional PI for the Center of Marine Biotechnology. He
will be responsible for co-coordinating the biological experiments undertaken at COMB. He is a
pioneer in the study of halophilic Archaea and a well-known expert on microbial genomics,
transcriptomics, and bioinformatics. He is leading several key experiments that will provide
insights into adaptation of halophiles to low temperatures and resistance to damaging radiation,
in collaboration with DeVeaux, and McCready.
Prof. E. Turner (Princeton University) is the institutional PI for Princeton and will be responsible
for coordinating the astrophysics and engineering projects undertaken there. He is a widely
experienced optical astronomer who will work on innovative ground based observations of
exoplanets, Earthshine and Solar System objects. He has also contributed to the investigation of
potential exoplanet biosignatures.
Prof F. Chen is an expert on marine cyanobacteria and cyanophage, and will be responsible for
testing the cold and radiation tolerance of cyanobacteria isolated from various sources, including
some samples collected from Antarctic.
Dr. C. Christian is highly experienced in scientific outreach, and has pioneered the use of
information technology for educational and research activities. She will co-ordinate the E/PO
activities of this project.
Prof. C. Chyba is widely recognized as an intellectual leader in the field of astrobiology and has
made significant contributions on a wide variety of topics, ranging from SETI to cometary
delivery of volatiles to objects in the inner Solar System. He is an expert on possible Europan
and Martian biology, which will be the foci of his efforts supported by this project.
Prof. N. J. Kasdin is an expert on systems engineering and advanced optical technology and
instrument design. He leads the Princeton high-contrast coronagraphy group and will coordinate its activities internally and in interactions with the rest of the team.
Prof. M. Littman is an optics and robotics expert who will have primary responsibility for
laboratory optics work at Princeton.
Dr. M. Livio has an extensive resume of past work on galactic, stellar and planetary astronomy.
He will provide input primarily on Galactic habitability issues, and will also participate in the
outreach program.
Dr. A. Nota has extensive expertise on stellar evolution, and will be a key science contact in
developing the Solar Neighborhood Habitability Census.
Prof. B. Paczynski is a pioneer of the study of Galactic microlensing and of its application as a
technique for detecting exoplanets and is the intellectual leader of the OGLE project. Working
with Princeton graduate students he will use OGLE data to study the statistical properties of the
exoplanet population in the Galaxy
Confidential
Page 64
6/20/2017
Dr. M. Postman will provide project management oversight. He has extensive experience in
database construction, and will participate in construction of the SNH Census.
Prof. F. Robb is an expert on Archaea and thermophilic life. He will lead the study of microbial
survival on minimal energy and carbon sources, during radiation challenges and under cyclic
temperature variations. He and collaborators Onstott, Steele and Fogel will study microbial
enrichments from Arctic and subarctic Mars analog sites.
Dr. D. Soderblom has extensive expertise on nearby stars, particularly chromospheric and
coronal activity, and will be a key science contact in developing the Solar Neighborhood
Habitability Census.
Prof. K. Sowers is an expert on the physiology and molecular biology of the methanogenic
Archaea. He will be responsible for all experiments proposed with for the Antarctic
methanogens. He is also a collaborator for the genome sequencing projects for both
psychrotolerant methanogens and will collaborate with R. Cavicchioli, a leader in proteomics of
the psychrophilic Archaea, to investigate the molecular mechanisms of cold adaptation
Dr. W. Sparks is an expert on elliptical galaxies. He is currently investigating the potential of
circular polarization as an unambiguous biosignature; he will lead work on the development of
the field polarimeter.
Prof. D. Spergel is one of the country’s leading theoretical astrophysicists and has worked on an
uncommonly broad range of topics, ranging from early universe particle cosmology through
Galactic dynamics to advanced optical theory. He will work on both optics and theoretical issues
related to panspermia in this project.
Prof. S. Tremaine is an expert in theoretical dynamics and has worked extensively on problems
of Solar and exoplanetary system dynamics as well as planet formation theory, the areas of his
involvement in this project.
Dr. M. Turnbull is currently an NAI Fellow at the Department of Terrestrial Magnetism of the
Carnegie Institution of Washington. She has extensive experience in analyzing planetary
habitability, and on measuring Earthshine. She will be based at STScI and will work on both the
Earthshine project and work building and testing the Solar Neighborhood Habitability Census.
Dr. J. Valenti is an expert in stellar activity and stellar chemical abundance analysis. He will also
provide information on the detailed properties of the host stars of known extrasolar planetary
systems.
Prof. R. Vanderbei is an expert in numerical optimization and optical designs who has made
fundamental contributions to the theory of high-contrast coronagraphs. He will work on these
topics as a part of the coronagraph work at Princeton.
Confidential
Page 65
6/20/2017
7. Strengthening the NAI Community
E/PO
Professional
community
development
Training
Minority institutions
Staff participation
Facilities
Flight missions
Information
technology
7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 8.1 8.2 8.3 8.4 8.5 8.6
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
x
7.1 Strengthening the Astronomical Connection
Our astronomical team members bring to the NAI a wide range of expertise, both technical and
scientific, in astrophysics and space sciences. Our team includes experts on cosmology, stellar
evolution, stellar populations, Galactic structure, extrasolar planets, planetary formation and
dynamics, brown dwarf and planetary atmospheres, planetary environments and Solar System
objects, including Europa, Titan and Mars. In particular, several CoIs are involved in research
covering a broad span of techniques for detecting extrasolar planets. This diversity is essential
for understanding the Galactic landscape, the first milestone on the Astrobiology Roadmap.
The Space Telescope Science Institute is one of the most prestigious astrophysical institutes in
the United States and is renowned for its public outreach efforts related to the Hubble Space
Telescope. A recent review of the NASA Astrobiology Institute by the Committee on the Origin
and Evolution of Life (COEL) of the Space Studies Board highlighted STScI as a potential
catalyst for creating an astronomical influence in astrobiology. Through its role in HST, STScI
has close contacts with the astronomical community, and it has used those contacts to engage the
community in astrobiological issues, notably through the 2002 (`Astrophysics of Life’) and 2005
(`A Decade of Extrasolar Planets’) May symposia.
Princeton University has a long and storied involvement in astrophysics. In recent years,
Princeton was one of the prime movers behind the Sloan Digital Sky Survey, surveying 
steradians of the high latitude sky. Princeton is closely involved in technical and scientific
developments in planet detection, and is currently establishing a Center for Planets and Life. The
University is also in the process of developing a certificate program in astrobiology that will be
offered to undergraduate students.
Confidential
Page 66
6/20/2017
7.2 The Biology Connection
The Center of Marine Biotechnology, one of five research centers of the University of Maryland
Biotechnology Institute, conducts biotechnology research in the context of the marine
environment; provides training and public outreach to various local, state, national, and
international audiences; and encourages economic development within the State of Maryland.
Housed in modern state-of-the-art laboratory facilities at the Columbus Center in Baltimore’s
Inner Harbor, COMB is conveniently located within a short driving distance of STScI. COMB’s
mission is to apply the modern tools of biology and biotechnology to study, protect and enhance
marine and estuarine resources. Among the 40 members of the COMB faculty is an outstanding
group of investigators with expertise in a wide range of extremophile biology. With extensive
research publication records in extremophile genomics, physiology, biochemistry, and molecular
biology, these laboratories cover the lifestyle spectrum of extremophiles, life in extreme heat and
cold, high ionic strength, and in oxygen-deprived environments and play a leading role
internationally. As co-editors on the Cold Spring Harbor Laboratory Press Handbook, Archaea:
A Laboratory Manual, they have sponsored annual symposia and workshops on extremophile
biology. As a marine research institution, COMB is fortunate to partner with Maryland SeaGrant
in a variety of education and outreach activities ranging from curriculum development to teacher
training and school-based programs.
CoIs DasSarma, Sowers and others are supported through the NSF and DOE Microbial Genome
Programs, while Robb, Chen and other members of the COMB faculty are funded to be
participants in the Microbial Observatory Program. Together with collaborators like Andrew
Steele and Marilyn Fogel at the Carnegie Institution of Washington and Richardo Cavicchioli at
UNSW, they have access to a wide variety of extremophilic species and genome sequences
suitable for analysis as part of our astrobiology program. Over the last two years, COMB and
STScI personnel have been engaged in active scientific collaboration, placing the biological
investigations in an astronomical context. The results of these experiments prompted some of the
research outlined in the present proposal (see section 4.2.4), and they have been submitted for
publication in the journal Astrobiology.
7.3 Communicating astrobiology in the research community
While there remains some skepticism about Astrobiology in the Astronomical and Biological
communities, we are committed to bridging these “culture” gaps through focused outreach at
professional meetings in our respective home fields. For astronomers, the most significant
meetings are the American Astronomical Society winter meetings, and as an NAI team we will
engage the AAS organizers in creating an “Astrophysics of Life” symposium. This symposium
will be lead by early career and senior astronomers (including PI Reid and CoI’s Turnbull,
Valenti and Livio) who will introduce their colleagues to the outstanding questions and latest
findings in Astrobiology.
The American Society for Microbiology has been and will continue to be a valuable vehicle for
communicating the importance of astrobiology. At the 2005 ASM General meeting in Atlanta,
CoI DasSarma gave a symposium lecture on astrobiology; at the 2006 Orlando meeting, he will
serve as co-convener on an Astrobiology session and involving other members of the NAI team.
Confidential
Page 67
6/20/2017
We are intent on developing a genuine synergy between the biological and astronomical
scientists and as we described in our research plan, as a step towards that symbiosis we are
producing a compendium of scientific reviews, archived webcast presentations, and explanatory
articles to enhance our own research efforts. This material will be hosted at STScI and available
from the NAI web pages, and will greatly enhance the accessibility of our research to other
members of the NAI and the general scientific community.
We intend to continue to foster regional interest in astrobiology by organizing further workshops,
hosted at STScI, COMB and Princeton. Those meetings will cover a number of astrobiology
topics, including the biological impact of changing planetary environments, mapping life through
the Galaxy and novel techniques for extrasolar planet detection.
7.4 Minority Institutions
Collaborator Jochen Müller (formerly at COMB) has recently taken up a faculty position at
Morgan State University, Baltimore. We anticipate that undergraduates from that institution will
participate in some of the biological experiments outlined in this proposal.
7.5 Undergraduate Astrobiology Training
Exposure to astrobiology at the undergraduate level can significantly enhance the integrity of the
Astrobiology community by building a base of enthusiasm among the general public and by
recruiting future Astrobiology researchers. At Princeton, a group of 15 faculty members from 8
science and engineering departments at Princeton are engaged in an ongoing series of
educational initiatives intended to establish a major international center of activity in the
intensely interdisciplinary field of astrobiology. These include:
1. Teaching a “sophomore initiative” cross-listed course (GEO/AST/EEB/CHM 255: Life in the
Universe), currently being taught by Profs. E. Turner, C. Dismukes, L. Landwebber, T.C.
Onstott and E.I. Steifel.
2. Developing a certificate program in astrobiology, a proposal currently under consideration by
the University
3. 3. Supporting a new undergraduate organization (The Princeton Astrobiology Club, P-ABC)
recently established via student initiative.
The major educational component of this suite of initiatives is the Astrobiology Certification
Program (ACP). If approved, it will allow Princeton undergraduates to formally add a
“Certificate in Astrobiology” to their departmental concentration (major) on their diplomas by:
1. taking a special two semester astrobiology sequence (typically in their sophomore years),
2. completing relevant junior and senior year independent work projects on topics approved by
the ACP director and the student’s department of concentration,
3. taking four “cognate” courses (regular courses deemed important to Astrobiology, e.g.
biochemistry, biophysics, evolutionary biology, petrology, astronomy), two of which may be
in the student’s home department, and
4. participating in a senior thesis colloquium.
Confidential
Page 68
6/20/2017
The undergraduate program at Princeton will be augmented by a Joint Astrobiology Student
Internship Program connected to STScI and COMB under the mentorship of CoIs Valenti,
Turnbull and Robb. In a cooperative program, summer students (some of which we hope will be
recruited from the Princeton ACP) will have the opportunity to interact with one another at all
institutions. Students will work individually with mentors from each institution and they will
convene for weekly meetings, workshops and social activities at alternating locations between
STScI and COMB. In this way the students will have the opportunity to:
1. work closely with one mentor pursuing their own research project,
2. interact and socialize with other students at both their “home” institutions and in our
astrobiology consortium, and
3. gain cutting-edge cross-disciplinary exposure to the broad range of research done at STScI
and COMB
STScI and COMB will each support two interns; Princeton will support one student from their
regular summer internship programs.
7.6 Graduate and postdoctoral astrobiology training: The COMB
Extremophile Summer School
One of the NAI’s unique roles in the scientific community is that of providing scientists,
especially those in the early stages of their careers, opportunities to gain Ph.D.-level experience
in new disciplines. The STScI-COMB-Princeton consortium, in particular, can offer graduate
students, postdoctoral fellows, and other researchers a special opportunity to work first hand as
biologists, with a curriculum that is tailored to those who come from non-biology backgrounds.
We propose to host a “Extremophiles in Astrobiology: Theory and Techniques” (EATT) fiveday summer school at the COMB facility, open to graduate students, postdocs, and other
researchers who engage primarily in “non-biology” astrobiology research. The EATT
Workshop is intended to complement workshops held at other NAI institutions (e.g., the
University of Hawaii and the University of Arizona) that are intended to help life-science and
Earth-science students become more fluent in the languages of planetary- and space-sciences.
“What would you do if someone handed you a bag of rocks from Mars?” While most
astrobiologists who work in the fields of atmospheric, geological, planetary, or space science
would love to know whether samples collected (or investigated in situ) on other planets contain
biological activity, very few would have any idea where to begin with such a question. Indeed,
most scientists who were not “raised” as biologists have a difficult time understanding the
language of biologists and the techniques they use to characterize microbial life on Earth, and
this adds to the struggle in keeping up with exobiology research. Getting a handle on which
techniques provide what information and are subject to which limitations is several steps beyond
the biology expertise of most space/planetary science Ph.D.’s. The EATT summer school is
designed to start filling in these gaps of understanding in an engaging environment that fosters
new relationships among participants. At the EATT summer school, participants will engage
in: (1) hands-on biology lab experience, (2) seminar-discussion sessions focused on
extremophile research and pitched to the level of a non-expert audience, and (3) the
opportunity to see and use COMB’s unique facilities for the study of anaerobic
biochemistry and molecular genetics.
Confidential
Page 69
6/20/2017
To create a summer school program that is appropriate for NAI and other astrobiology scientists,
we have taken the highly successful curriculum that our COMB CoIs DasSarma, Sowers and
Robb developed, used, and refined over the last decade, and adapted it for an interdisciplinary
Ph.D.-level audience. The course description and curriculum are as follows:
EATT: Objectives
The “Extremophiles in Astrobiology: Theory and Techniques” course will be held during
the summer at the University of Maryland Biotechnology Institute’s (UMBI) Center of Marine
Biotechnology (COMB) teaching labs in the Columbus Center, Baltimore Maryland. This course,
which includes a one-and-a-half-day symposium, three days of intensive laboratory experience, and
an afternoon for students to share their own research through poster presentations, will be
administered by the Office of Continuing and Extended Education, University of Maryland College
Park in association with COMB. The symposium will bring together experts from astronomy and
from various areas of extremophile research to discuss the current state of knowledge of growth,
biochemistry, genetics, ecology and diversity of microorganisms that thrive in unique environments,
and to place that research in an astrobiology context. Taking advantage of the expertise developed at
STScI, we envisage that at least a portion of the symposium will be webcast. The laboratory sessions
include lectures and blocks of demonstration and hands-on experiments focusing on specialized
techniques used to study these microorganisms.
EATT: Workshop Schedule
Our NAI consortium’s “Extremophiles and Astrobiology: Theory and Techniques” has been
adapted from a summer course offered each year at COMB since the summer of 1997. We envisage
the course lasting a week, with an all-day symposium on the opening Monday, and on the Friday
morning, with the afternoon given over to student poster presentations. The central part of the week is
devoted to hands-on laboratory experiments.
The first-day symposium will be introduced with a presentations and discussions that provide, from a
space scientist’s perspective, the astrobiological context of extremophile research. The symposium
talks will generally be 50-55 minutes in length, with 5 minutes for questions. Invited speakers will be
drawn from the general community, but we anticipate that both STScI and Princeton will contribute
speakers on the “astro” aspects of astrobiology. Each speaker devotes 30 minutes to general aspects
of their research area with the remaining time dedicated to their own research specialty. Reid,
Turner, and Turnbull will work specifically to make sure that all of the material presented to students
is accessible and relevant to those who are building careers in non-biological sciences.
In the experimental section of the workshop, each day is divided into three 2.5-hour
modules, one each for halophiles, methanogens and thermophiles, allowing participants to be
divided into four groups of three to four. The groups then rotate through the modules. The
faculty presiding over the modules are experts in their particular field and are assisted by
graduate students and/or postdoctoral students who work in their laboratories.
Confidential
Page 70
6/20/2017
With specialized facilities for carrying out extremophile research, COMB is the ideal location
for this workshop. COMB houses dedicated anaerobic laboratories that are specially equipped with
instruments for conducting both small and large-scale batch and continuous culturing of strict
anaerobes as well as biochemistry and molecular genetics under strictly anaerobic conditions. The
laboratories, including the two teaching laboratories used in this course, are plumbed for the
distribution and mixing of anaerobic gasses at several bench stations. The facility also contains
apparatuses for preparing anaerobic media, anaerobic gassing manifolds, and Coy anaerobic
chambers: one chamber equipped for anaerobic microbiology and the other refrigerated and equipped
with a dedicated HPLC for anaerobic biochemical separations. COMB also houses a unique
fermentation facility, the Extremophile Scale-Up Facility (ExSUF) that is equipped with a customized
pilot-scale 250-liter fermentor and 20-liter seed fermentors adapted for growth of strict anaerobes and
hyperthermophilic microorganisms at temperatures up to 105°C.
The support we are requesting to fund this workshop includes costs associated with about 100
participants for the “theory” portion, and 16 students for the hands-on “techniques” segment. Funds
are requested for laboratory supplies, our course textbook (Archaea: A Laboratory Manual
published by Cold Spring Harbor Press), logistical costs (for the symposia and field work), and
instructor and student travel and lodgings support. As discussed in detail in budget narrative, we are
requesting a total budget of $40,000 per workshop, and we anticipate holding workshops in years 1, 3
and 5.
EATT: Target Audience
The ERTT workshop is intended for advanced graduate students, postdocs and other researchers
who have begun their own research program in a non-biology-focused, but astrobiology relevant,
field. Early career participants will be given priority for attendance and financial support, but
researchers at all stages of their careers will be invited to attend provided there is room. Funding
will be provided only to graduate students and postdocs, and all funded participants will be
expected to contribute a poster in our afternoon poster session. We envisage the experimental
section of the workshop accommodating up to 15 participants.
EATT: Promotion
Marketing for the workshop will be done via NAI electronic newsletters, print media
announcements, announcements and fliers at the Astrobiology Graduate Conference, the
Astrobiology Science Conference, the Division of Planetary Science meeting, the American
Geophysical Union, and other space, planetary and geological science meetings, as well as
meetings of the American Society of Microbiology. Turnbull will also distribute information via
the early career astrobiology email list started at the first Astrobiology Graduate Conference
sponsored by the NAI and via the Ames Academy Alumni listserv. Finally, fliers will be
distributed to NAI nodes for posting.
7.7 Astrobiology Postdoctoral and Graduate Fellows
Our proposed research program will also involve the direct participation of at least 6 graduate
students and 5 postdoctoral fellows. Most of those personnel will work either at COMB, on
biological experiments, or at Princeton University. We anticipate that two postdoctoral fellows
Confidential
Page 71
6/20/2017
will based at STScI, working on developing the SNH Census and on the construction and
implementation of the circular polarization field instrument. We also will take advantage of the
summer student program run by STScI to involve undergraduate students in astrobiology
research. Princeton University faculty members participating in this proposal will incorporate
aspects of this research and astrobiological concepts in their undergraduate and graduate courses,
and CoIs from COMB/University of Maryland are actively involved in teaching graduate and
undergraduate courses covering a range of levels, and topics from ecology to bioinformatics. We
anticipate that our research tools will be used in a number of those courses.
7.8 Astrobiology and relevant NASA Flight Missions
The research program outlined in our proposal is directly relevant to several of the most
prominent space missions currently in preparation by both NASA and ESA. Those include
KEPLER, SIM Planetquest, TPF, ESA’s DARWIN mission and, eventually, Lifefinder. Our
team members have extensive involvement in those projects: Collaborator Gilliland is a CoI in
the KEPLER project; PI Reid is a member of two SIM Planetquest Key project teams; and CoI
Sparks was a member of the TPF Science Working Group. All of these missions are directed
towards identifying and studying extrasolar planetary systems.
As discussed in our proposal, KEPLER is a wide-field imaging instrument that will search for
transiting planetary systems. The results from that project will provide significant new
information on the frequency and characteristics of sub-Jovian mass gas giants and terrestrial
planets. In contrast, SIM, TPF and DARWIN are all pointed missions, designed to target a
relatively small number of sources. This is particularly the case for TPF and DARWIN, missions
that will devote a substantial fraction of their total program effort to observations of ~100 stars
within the immediate Solar Neighborhood (d < 20 parsecs). Clearly, it is vital to select the best
possible targets for those programs. The research program outlined in this proposal will provide
a quantitative framework for addressing exactly this issue.
The Solar Neighborhood Habitability Census will catalogue both empirical data and the derived
intrinsic properties for stellar systems near the Sun, including those stars that will be considered
candidates for detailed study by TPF and DARWIN. We will be able to use the statistical tools
created as part of our research program to combine relevant information to yield a ranked list of
nearby systems most likely to host life. Some systems have known extra-solar planets in the
habitable zone, while others have companions that preclude stable planetary orbits in any
habitable zone. The remaining stars will not be perfectly characterized, but we will still be able
to rank them on the basis of statistical trends observed in system with known extra-solar planets.
The optimal biomarker to use when searching for life will depend on the expected environment
for each system being studied.
All team members at STScI and many of the Princeton CoIs have extensive experience with
NASA flight missions. Most STScI personnel are involved on a daily basis with operation of the
HST, and have participated in HST servicing missions and the development cycle of new
instruments. Some are already working daily on the James Webb Space Telescope, which will be
an important tool for astrobiology. Our experience with current and future NASA flight missions
will naturally guide the focus of the research tools we develop in this project, making those tools
more useful for planning and executing future NASA flight missions.
Confidential
Page 72
6/20/2017
8. Education, Outreach and Press Presence
8.1 Capturing public imagination: Education, Outreach and Public
Understanding of Science
Imagine sitting before a home computer and using a pair of affordable stereo vision goggles,
calling up an image of a starfield. Suppose you could fly about the scene to see a stereo
rendering of the various components, navigating around stars, star forming regions and dark
areas of hydrogen and dust. Highlighted areas point out planetary systems in various stages of
formation. Zooming in on a particular region, you see a star system being born. Perhaps you are
interested in calling up the detailed explanation of one of the planets in that system that caught
your eye. You click a button, or perhaps issue a voice command and a description of the planet
appears, superimposed on the view like an heads-up display. What kind of microbes might live in
that environment? Lets find out!
The public as well as students and teachers will discover with us the types of planetary systems
that are known and how to detect new systems through emerging observational techniques. Our
public audiences will be able to characterize the nature of exoplanetary systems found and
speculate on the types of environments present in those systems. We will investigate the variety
of conditions for survivability and adaptability of potential life forms using our research on
extremophiles as a guide. By putting the two together, that is, the astrophysical and biological
analyses, our public explorers will be able to experience for themselves the possibilities for
habitable environments in the Solar Neighborhood. Our multifaceted outreach program will
cultivate exploration and study by collaborating with existing programs both within the NAI and
the general science education community.
We acknowledge that the fundamental questions embedded in astrobiology arouse the
imagination – How did life arise? Are we alone? What is our future? We will use that fascination
as a springboard for our education and outreach activities. We will employ the tools of
instructional technology -- the Web, the Internet, and the media coupled with existing
educational resources and networks to create a direct access to the investigations.
We will approach Education, Outreach and Public Understanding of Science (referred to within
this text as EPO) through several complementary activities. We will create our Astrobiology
EPO program to take full advantage of the long established education and public infrastructure
experience and infrastructure of STScI’s Office of Public Outreach (OPO), COMB’s expertise in
teacher research collaborations and professional development and Princeton’s success with
astrobiology graduate and undergraduate education. We understand that to be most effective, our
program cannot be built from “scratch”, especially given the limited funding allowable for this
proposal. We also have been selective about which areas of EPO we will pursue. We will
collaborate with the skilled and expert staff of OPO, use existing networks, and distribution
channels as well as link to related resources, background material and curriculum materials to
maximize the impact of our efforts. We already have ties to several groups within the NASA
Astrobiology education community through the NASA Origins Forum, managed by STScI/OPO.
Our spectrum of activities (Table 8.1) will be well coordinated with the NAI and NASA
Confidential
Page 73
6/20/2017
Education Support Network. In this EPO section we discuss up through K-14 education. The
college level education is shown in the Table for completeness, is discussed elsewhere in this
proposal, and emphasizes Princeton’s role in undergraduate and graduate astrobiology education.
In brief, our “ExtremoFiles: Notebook” will highlight the progress of our research along with
salient updates, news notes and occasionally, press releases as our research progresses, and
conduct experiments in the biological laboratories. This area also includes “Kids News” from the
Amazing Space suite of resources.
“ExtremoFile Explorer” is a collaborative project with the National Virtual Observatory
(NVO), OPO, and ManyOne Networks to create two-dimensional imagery and three-dimensional
renderings of our local solar neighborhood from astronomical survey data available in the NVO
such as the Sloan Digital Sky Survey, other sources, and our own Habitability Census data
resulting from the research in this proposal. We will represent the stellar population attributes
down to the local elements containing planetary systems and the potential for hosting microbial
organisms. The 2-D imagery will be folded into modules usable for planetarium presentations,
science museum kiosks and Web-based public resources where the public can join us in our
explorations. We also will sponsor lectures open to the public at our sites and at museums and
broadcast via webcast technology over the internet.
Table 8.1 Spectrum of EPO Activities, described in detail in the following sections.
Type of Outreach
News
Public Information/
Outreach
Informal
Education
K-14
support
Science
curriculum
College/ University
curriculum
Element
ExtremoFile
Notebook
Typical Products
News releases
Media
contacts
interviews
Web resources
Linked resources
Paper products
Self-guided modules
Content
News text, images, mediated &
/ derived products, multimedia,
biographical sketches
ExtremoFile
Mediated products, images, lab
Explorer
notes, 2-D and 3-D solar
neighborhood model hypertext
linked
to
exoplanet
characteristics
ExtremoFile
Exhibits
Images, models, laboratory
Explorer,
Planetarium shows
materials, 2-D and 3-D solar
ViewSpace
Kiosks
neighborhood models
hypertext linked to exoplanet
characteristics
Laboratory,
Links to Curriculum Images,
processed
data
Teacher
modules, Self-guided products, science data and
Research
modules
parameters, models, hands-on
Fellowships,
Teacher Workshop
laboratories
Living in the Teacher Professional
Extreme
Development
Astrobiology
Courseware
Images, science data, processed
Certificate
Self-guided modules
data products
Projects
Relevant formal education curriculum support tools already developed for use in classrooms at
STScI and COMB will be linked to our program through our “ExtremoFile Laboratory
Researcher” initiative. The educational resources are in use nationally and reach a significant
Confidential
Page 74
6/20/2017
segment of underrepresented and home-schooled students in all 50 states. All products developed
for classroom use are linked to national science, technology engineering or math standards and
Project 2061 Benchmarks, integrated into ongoing NASA OSS education activities and subjected
to rigorous review and formative evaluation processes. We will disseminate late-breaking
discoveries into the classroom through established outlets at NASA, NAI, STScI and COMB.
Each of these areas is discussed in detail below. In the descriptions we articulate our
commitment to wed the EPO activities directly to the research being conducted and the
investigators who drive the program.
8.2 ExtremoFiles: Notebook
Communicating with the Public. We know there is tremendous public interest in the origin of life
and the tantalizing possibility that life exists elsewhere in the solar system and galaxy. We will
capitalize on this curiosity and stimulate the public interest in our Astrobiology project by
communicating the latest information on our studies to the public and educators through our
many faceted project website. We will create a window into our Laboratory (a virtual
astrobiology visitor center) to showcase the progress of our experiments and our models of the
solar neighborhood. Through vignettes into our scientific “laboratory notebooks” we will
describe online, using current technologies such as weblogs, RSS feeds and the like (see Figure
A), the context of our work, our strategies and the latest results. We will illustrate our Notebook
with simple graphical representations of our models, imagery, straightforward explanations and
background material covering the nature of stars, galaxies, planetary systems and adaptive
biological organisms. This part of our project website will be developed by the Office of Public
Outreach (OPO) at STScI.
Alerting the Public through the News Media. We occasionally will prepare and distribute press
releases with the News Group of OPO, a group that has excellent professional rapport with the
media and a long history of accurately communicating space science to the press. As is our
natural practice at STScI, we will coordinate with the home institutions of our collaborators and
in this case, also the experience and leverage of the NAI. All press materials will also be
available electronically through the Notebook portion of our website as well as linked through
OPO, NASA (as appropriate) and our collaborating institution. We will include a “kids news”
area based on OPO’s Star Witness feature (amazing-space.stsci.edu/news/). The content provides
online reading selections mirroring the content of press releases, but for grade school students to
enjoy. Teachers can elect to address reading concepts using passages that expose the students to
new science ideas.
We also will offer live interviews, Q&A sessions, animation, graphics, textual information and
imagery captured and rebroadcast on the Web for use in schools, libraries, science museums and
by the interested public. Major scientific findings (anticipated to be a few over the course of this
project) will be showcased through NASA press conferences, the Space Science Updates,
produced by STScI and broadcast from NASA headquarters on NASA TV, accessible on cable
networks and by classrooms across the country.
Live from ExtremoFiles. We host public lectures regularly at our all research sites through “Open
Night” type events. We also participate in other public events, such as panel discussions, lectures
Confidential
Page 75
6/20/2017
Figure A. Sample layout
for the SkyWatch website,
our working model and
adaptable for the
ExtremoFile Notebook ,
see
hubblesite.org/skywatch.
The site contains links to
relevant topics, RSS feed,
links to media and
researcher biographical
profiles and serves as a
template for our Notebook
portion of the website.
at various institutions around our local areas (such as Maryland Science Museum, Air and Space
Museum, etc.) and are regular contributors on National Public Radio such as WYPR’s Marc
Steiner Show and NPR’s Science Friday. C. Christian is also the co-host of WYPR’s SkyWatch
an astronomy radio vignette aired once a week and available through RSS feeds and podcasting
(Figure 8.1, above), and will highlight our research advances in that venue as well. We will take
all those opportunities bring the flavor of Astrobiology to the public. We intend to capture those
events in our webcast archives, so that local events can reach a much wider audience. We will
link those resources to the interfaces of the NAI such as the “Feature Stories” and the “Spotlight”
section.
8.3 The ExtremoFile Explorer
Visualizing Solar Neighborhood Data and Models. The data available in astronomical archives,
accessible through the NVO, the Habitability Census, and models produced in the course of the
research described in this proposal form the core of our activities in the Public Understanding of
Science. Using our scientific modeling tools and observational data as a catalyst, we will create
two-dimensional imagery and a three-dimensional scientific visualizations of our model solar
neighborhood, down to the microbial level, collaborating with the OPO Visualization Lab.
In addition, we will couple our data with existing 3-D tools available with simple viewers and
browsers as well as stereoscopic goggles when possible. We make a distinction here between
visualization, described above as produced by experienced animators, and the 3-D tool we will
make available through a web browser. Both portray our data and models. In the one case,
visualizations can be used in a variety of contexts including informal science venues, as well as
classrooms. The 3-D web tool is provided by Digital Universe (DU) from ManyOne Networks, a
collaborator with the NVO. The free browser tool allows the user to roam through the universe
and select different scales for viewing. We will be “stewards” of the astrobiology content in the
DU for the solar neighborhood, in that we will be able to add, as our research progresses, details
Confidential
Page 76
6/20/2017
of planetary systems and the possible life forms they can sustain, directly as links in the tool.
This model can be thought of as a visually enhanced sort of Wikipedia, with robust and reliable
content and authorship. Users will roam through the universe at will, and when virtually
traveling through the solar neighborhood, will be able to link to supplemental information.
Therefore, ManyOne Networks provides the tool, and we synergistically add value to the tool
with information derived from our research results. We also intend to provide our data to the
Planet Quest developers at JPL to include in their 3-D planetary systems tool, to ensure
widespread discussion. The Digital Universe tool allows users to browse freely through the 3-D
universe, while Planet Quest focuses on a sun-centric region, hence the two are complementary.
Museum and Planetarium Displays. The visualizations produced from our scientific work will be
offered as support materials to museums and planetariums along with the 2-D imagery. The
materials can be integrated into projections for use by museums such as Baltimore’s Davis
Planetarium, Alder and the Rose Center at the American Museum of Natural History. There,
visitors will experience virtual space flight through the solar neighborhood, guided by the
educator at the planetarium, describing the nature of galaxies, stars, and the astrophysical
processes depicted. We will offer materials along with the other informal science modules
through hubblesource.stsci.edu. As is our practice, we will coordinate with representative
planetarium staff and other educators to insure resources are available in a wide variety of
formats for distribution to other organizations, for example, under the auspices of the
International Planetarium Society.
Again with OPO, we also will produce modules for ViewSpace, OPO’s highly acclaimed
multimedia product, tuned for museum kiosks, plasma screen displays, video booths, and
planetarium shows and distributed widely by OPO to informal science centers. We will
collaborate with OPO to provide content for STScI’s regular distribution of visualizations, video,
imagery and graphical content to planetariums, documentary producers, textbook authors, and
more through hubblesource.stsci.edu. ViewSpace modules comprise high-resolution images,
digital movies, animation, and interpretive captions with evocative space music as a background,
with content produced by a variety of astronomical facilities. The ViewSpace modules play on
normal monitors, projectors, and also on high-resolution wide screen plasma displays.
ViewSpace is available at about a hundred museums around the country, and OPO regularly
provides updates to the program.
ExtremoFile Explorer Website. Our Explorer aspect of the website will encourage the public to
ask basic questions about the abundance, distribution, and types of life that may exist within our
nearby neighborhood. Users will address questions through carefully crafted form-based
interfaces developed as straightforward interfaces into the ExtremoFile Laboratory. Users will be
able to select combinations of environmental parameters to discover the planetary systems that
can host various configurations and the nature of the microbial life that can survive on planets
and other host surfaces. We realize that to be successful, the ExtremoFile Explorer element of
our website needs to be augmented with material that provides a useful context appropriate for
the public audience. We will craft our most significant threads of inquiry to guide thinking by
non-experts and motivate further reflection. Our goal is develop a public appreciation of the
status of our knowledge on this subject as well as gaps in our knowledge. We will encourage the
Confidential
Page 77
6/20/2017
public to appreciate the types of experiments and observational data that must be acquired, and
gain an additional insight into the dynamics and discovery nature of our investigations.
We will create a linkage from our project to the National Virtual Observatory (NVO). The NVO
EPO program collaborates with existing projects to bring real scientific data into the classroom
and science centers. We will insure that our astrobiology project will coordinate relevant
materials with NVO interfaces and distribution mechanisms to insure national and international
access to our findings.
8.4 The ExtremoFile Laboratory
We intend to share the planning, progress, and evolving results of our project with the public
through cogent, understandable content based on our unique research, and appropriate for public
audiences. The principal foci of our EPO activities and resources are informal science education,
public information and public access as described in the previous sections, which complement
the student and teacher activities already extant in the NAI suite.
Our formal education support primarily will be links to existing, working, proven, curricula
supplements such as Amazing Space from STScI, the COMB Microbes for Hire, Celestia, and
other resources aimed at enhancing science literacy. We wish to link to specific resources
available through the NAI such as Microbial Life Education Resources, Alien Safari and others.
Our resources will further highlight our data, models, and the derived results so the viewer
(individual, student, educator) will be able to appreciate the nature of our work in a general
astronomical context. In the longer term and through the NVO EPO collaboration, we can
provide data and information to our partners, such as CLEA’s2 Virtual Education Observatory
where students virtually obtain astronomical data, Project Lite3, from Boston University which
uses spectroscopy to explore the nature of stars, galaxies, and nebulae and the Adler
planetarium’s professional development classroom4.
8.5 ExtremoFile Educators
In keeping with our EPO philosophy to take advantage of collaboration with existing projects,
we will partner with the UMBI BioScience Education Center and Maryland Sea Grant Extension
program to develop lab-based student experiences, teacher professional development
opportunities, and leverage our current teacher research fellowship programs to improve the
understanding of the field of astrobiology. We will encourage studies of extremophiles biology
and their potential benefit for society, and help students, through their teachers, appreciate how
extremophiles could live in other habitable environments in the solar neighborhood.
We will capitalize on the vibrant research in the mid-Atlantic region on extremophiles and the
expertise of the University of Maryland in teacher development, and enhance teachers’ practical
experience in interpreting research information on this subject. We recognize that teachers are
not confident in performing laboratory activities related to extremophiles. Table 8.2, derived
from the science section of the proposal, illustrates the variety opportunities of educational focus
and the relation to astrophysical environments.
2
http://www.gettysburg.edu/academics/physics/clea/CLEAhome.html
http://lite.bu.edu/
4
http://www.adlerplanetarium.org/cyberspace/nvo/ - class_prog
3
Confidential
Page 78
6/20/2017
With COMB in the leadership role, we will develop hands-on lab-based experiences (described
below) as professional development for teachers that emphasize the value of studying
extremophiles and their unique characteristic role in astrophysical contexts. Through programs
that are initiated with teacher research fellowships at COMB and STScI, scientists and teachers
will use the best resources currently available from our home institutions and the NAI to insure
that astrobiology and extremophile research finds a path to the classrooms in the mid-Atlantic
region, tied to educational standards in science, math and technical literacy. We intend to share
our lessons learned and resources through exchange of materials and information to enhance our
program and the entire suite of existing teacher and student programs in the NAI.
Table 8.2. Extremophile Characterization.
Environment
Moderate: Classic habitable zone
Heat: inner planetary orbits
Extreme heat: close proximity to host star
Cold : more typical planetary environments
Acidic: early terrestrial biosphere, e.g.
Salty: oceans, pods
Alkaline: e.g., host springs
Radiation: typical early environments
Toxic: typical early environments
Pressure: planetary interiors
Drought: typical, e.g., Mars
Rock dwelling: terrestrial planets
Near starvation: probably typical
Environmental Limits
T~ 20 - 50 oC
T ~ 50 – 75 oC
T ~ 80 - 105 oC, Tmax~90 - 115 oC
o
C for given species
T ~ -15 or below to 20 oC
pH at or below 3
requires at least 0.2M salt (up to 5.2 M)
pH > 10
-2
UV
substance dependent tolerance
up to 1.1 tons cm-3
low water activity: aw < 0.96
resident in rock
low nutrients
Species
Mesophiles
Thermophiles
Extremophiles
Hyperthermophile
Psychrophile
Acidophile
Halophile
Alkaliphile
Radiation tolerant
Toxitolerant
Barophile
Xerophile
Endolith
Oligotroph
8.5.1 Teacher Research Fellowships
We will offer teachers the opportunity to become a research fellow through two established
teacher research fellowship programs; the Chesapeake Teacher Research Fellowship
(www.esep.umces.edu) and the VIP Expert Program (www.scienceinquiry.org) made possible
with support from the NOAA Chesapeake Bay Office, the NSF and the National Park Service.
Teachers will be immersed in research with COMB and STScI scientists focused on extremophile and astronomical research and ultimately apply practical ideas in the classroom setting with
students. They will share ideas through the NAI as exemplary resources for their peers. We will
collaborate with the teacher fellows to insure they have opportunities to make significant
contributions toward professional development resources that will cascade to the Living in the
Extreme Workshops. Fellowship teachers are invited to return to the workshop series in years 3-5
in order to assist in the “hands-on” components of those workshops. COMB CoIs have
participated in one or both of the Fellowship programs over the last 5 years.
8.5.2 ExtremoFile Teacher Professional Development
Living in the Extreme professional development workshops will emphasize the fundamental
understanding of extremophile biology through interactions with STScI, COMB, and Princeton
scientists. These workshops will offer the opportunity to perform “hands-on” lab experiences
developed for safe use in the classroom by education and outreach staff at The UMBI
Confidential
Page 79
6/20/2017
BioScience Education Center in partnership with MD Sea Grant. We intend that this activity
have synergy with the NAI Summer Teacher workshop, although as of this writing the content of
the latter workshop is not yet available.
We base the Living in the Extreme professional development workshop upon a previous
workshop, Microbes for Hire, sponsored by the Pfizer Foundation, The Foundation for
Microbiology, and Bell Atlantic-Maryland held in 1998, 1999, 2000 and 2003 at COMB. The
workshop hosted Maryland middle school and high school teachers and was designed to translate
the applied microbial ecology research at COMB into laboratory activities for teachers and
students in Maryland. In Microbes, teachers gained laboratory skills and enhanced content
knowledge on a variety of topics including bioluminescence, bioremediation and the
Winogradsky marine sediment colonial growth stimulation effect. Similarly, Living in the
Extreme teachers will gain experience with biological activity in relation to surrounding
environments, and be able to relate this knowledge to astrophysical contexts. Each topic area will
be formulated in collaboration with COMB and STScI scientists or graduate students to provide
the essential background of the research and followed by a "hands-on" labs that expose teachers
to new research techniques, and ideas for their classrooms.
We will also use the Living in the Extreme workshop as an opportunity to investigate cultivation
of extremophiles in science museums. We will partner with the Exploratorium in San Francisco,
an innovative museum that connects science with art and human perception.
The
Exploratorium’s hands-on science museum is visited by half a million people each year; their
website draws roughly 20 million different visitors per year. They have an active program for
training teachers to develop creative science curricula, and they also design and produce exhibits
for other science museums around the world. The Exploratorium recently launched the
“Microscope Imaging Station” (www.exploratorium.edu/imaging_station/about.html) that puts
museum visitors in control of research grade phase contrast and fluorescent microscopes to study
cultures of mouse stem cells, embryonic heart development in zebrafish and round worms.
Two members of the Exploratorium exhibits staff with formal biology training will attend the
Living in the Extreme Workshop. They will learn techniques for cultivating various extreme
microbes and the planetary environments in which they might be found. In follow-up
teleconferences and videoconferences, proposal team members will collaborate with
Exploratorium staff on the establishment of continuous cultures of model organisms in the
Exploratorium’s laboratory facilities and follow up with in situ visits to devise a plan for the
establishment of extremophile cultures and supporting astronomical content for an exhibit,
related to the Microscope Imaging Station. This work will build on the success of an NAIsponsored collaboration between UMBI and the Exploratorium on documenting extremophile
research in Uzon Caldera, Kamchatka, Russia, in the summer of 2005 (NAI Director’s
Discretionary Fund award to UMBI Collaborator Albert Colman). Exploratorium also has a long
history of collaboration with STScI for astronomical exhibits and a webcast series based on
servicing of HST.
Confidential
Page 80
6/20/2017
8.6 Evaluation
Formative evaluation of our EPO program will be directed by an evaluator from OPO at STScI.
The evaluation criteria will be integrated at the initiation of the program. Assessment of the
program will be effected through a number of activities including: observation of teacher
workshop participants during the preparation phase and follow-up with participants afterwards.
We envision interacting with typical web users at various meetings such as the National Science
Teachers Association and Association of Science and Technology Centers to probe the usability
and effectiveness of our resources in sit-down sessions in display booths at those meetings. We
wish to probe the preconceptions held regarding astronomical research on planetary systems and
extremophile research and the linkages between them. We especially intend to be attentive to
how our efforts are unfolding and uncover obstacles, barriers or unexpected opportunities that
may have emerged.
For summative evaluation we will work with informal and formal science educators who use our
resources and collaborate in our workshops. We will evaluate the extent to which our resources
stimulate usage of our astrobiology material in various forms of education. We will also take
advantage of built in measures of web sessions and threads to understand how each section of the
web resources is being used and the linkages users make between various areas.
8.7 Our Timeline
We will use a staged approach for our Education and Outreach program. We consider that in our
first year, we will be creating the basis of our project so that production of fully developed
educational materials would be premature. We briefly describe the timeline here:
Year 1: The EPO Lead (Christian) will become integrated into the overall NAI education and
outreach network, developing an understanding of what products and services are available and
in development and will share the team's plans for the future. The Lead will coordinate support
of other NAI EPO activities and provide unique resources as appropriate. We will initiate the
Live from the ExtremoFiles public lectures program, radio programs, and webcast archive.
Initial public information on especially the Notebook part of the website will be developed. We
will develop the initial evaluation program and metrics plan. We will also begin the teacher
fellowship programs to partner 4 teachers with COMB and STScI Scientists. We will populate
the Digital Universe with existing baseline habitability data.
Year 2: The EPO Coordinator/Lead will evolve the detailed education and outreach plan based
on current needs assessments of the overall NAI and NASA programs and the intended
audiences. The EPO team will develop public access requirements for science derived products
and initiate development of the ExtremoFile Explorer part of the website. The Notebook will
continue to carry the latest information and news on the project. Teacher fellowships and
professional
development
workshop
planning
will
continue.
Year 3: We will augment the public portal with enhancements to the ExtremoFile Explorer. The
2-D and 3-D visualizations will be initiated, based on preliminary data from the Habitability
Census. We will augment the Digital Universe each year as new data and information becomes
available. Evaluation on the website and the workshops proceeds. The Teacher Fellowship will
end at the conclusion of year three and planning and recruiting teachers from the Living in the
Confidential
Page 81
6/20/2017
Extreme professional development workshop will continue. The Notebook will be maintained.
Year 4 and Year 5: We will develop and disseminate planetarium and museum renderings of
science data visualizations. The team will create ViewSpace feature programs, other materials for
educational use and continue to make press announcements as appropriate. We will create links
with NVO during the last year of the project. Teacher fellows from years 1-3 will return to assist
with the implementation of the one-week Living in the Extreme professional development
workshops held at the COMB during the summer months of year 4-5. Enhancements to the
Digital Universe and the Notebook continue regularly. In the last year, the final evaluation report
will be generated.
8.8 Our Education Expertise
STScI, COMB and Princeton have histories and extensive experience in developing various
curriculum support materials for education. Our experience also extends to support of pre-service
and in-service teacher education at a variety of colleges and other educational institutions across
the country. OPO at STScI has established itself as a major leader in providing resources based
on NASA space science that are directly relevant to mathematics and science curricula. OPO
produces a complete suite (Table A) of educational and outreach materials from news materials
and support of media, to the public information site (hubblesite.org), to the formal education
support under the umbrella of Amazing Space, and informal science education resources.
At COMB, the Microbes for Hire Program is designed to give current and prospective middle
and high school teachers within Maryland the skills, experience and confidence that they need to
effectively integrate information and imaging technology with the science curriculum and to
teach an increasingly important area of science – biotechnology. The innovative program is part
of an ongoing commitment to public outreach and inquiry-based education by the University of
Maryland Biotechnology Institute’s COMB and Maryland Sea Grant College. They have
successfully provided hands-on experiences in marine biotechnology to over 15,000 Maryland
students and their teachers over the past few years in the Science and Technology (SciTech)
Education Program Labs located in the Columbus Center, Baltimore, MD.
Teacher Research Fellowship programs (VIP ExPERT and Chesapeake Teacher Research
fellowships) are well established and illustrate the value of the teacher-scientists relationship.
These programs also take advantage of the unique opportunity to establish a direct pipeline
between research scientists and classroom teachers that will continue beyond the period of the
grant once there relationships are formed.
Confidential
Page 82
6/20/2017
9. References
9.1 Research and management
Adams, F. C. & Spergel, D. N. 2005, Lithopanspermia in Star-Forming Cluster, AsBio, 5, 497
Allen, C.C., Jager, K.M., Morris, R.V., Lindstrom, D.J., Lindstrom, M.M., & Lockwood, J.P.,
1998, JSC Mars-1: A Martian soil simulant, in Proceedings of the Conference of the American
Society of Civil Engineers, American Society of Civli Engineers, Albuquerque, pp. 469-476
Allen, P.R., Koerner, D.W., Reid, I.N. & Trilling, D.E. 2005, The substellar mass function: A
Bayesian approach, ApJ, 625, 385
Allison D.G. & Sutherland I.W. (1984) A staining technique for attached bacteria and its
correlation to extracellular carbohydrate production, J. Microbiol. Methods 2, 93
Bandermann, L.W., Kemp, J.C. & Wolstencroft, R.D. 1972, Circular polarization of light
scattered from rough surfaces, MNRAS, 158, 291
Becher, B. & Cassim, J. Y. 1977, Effects of bleaching and regeneration on the purple membrane
structure of Halobaterium halobium, Biophys J, 19, 285.
Beja, O., Spudich, E.N., Spudich, J.L., Leclerc, M. & DeLong, E.F. 2001, Proteorhodopsin
phototrophy in the ocean, Nature, 411, 786
Beloin C, Valle J, Latour-Lambert P, Faure P, Kzreminski M, Balestrino D, Haagensen JA,
Molin S, Prensier G, Arbeille B & Ghigo JM. 2004. Global impact of mature biofilm lifestyle on
Escherichia coli K-12 gene expression, Mol Microbiol. 51, 659
Blackstock W.P. & Weir, M.P. 1999, Proteomics: quantitative and physical mapping of cellular
proteins, Trends Biotechnol 17, 121
Blakefield. M. K. & Harris, D.O. 1994, Delay of cell differentiation in Anabaena aequalis
caused by UV-B radiation and the role of photoreactivation and excision repair, Photochem.
Photobiol. 59, 204
Boetius A, Ravenschlag K, Schubert CJ, Rickert D, Widdel F, Gieseke A, Amann R, Jorgensen
BB, Witte U & Pfannkuche O. 2000, A marine microbial consortium apparently mediating
anaerobic oxidation of methane, Nature 407, 623
Bond, I. A. et al. 2004, OGLE 2003-BLG-235/MOA 2003-BLG-53: A Planetary Microlensing
Event, ApJ, 606, L155.
Bustamante, C., Maestre, M.F & Kelle, D. 1985, Expressions for the interpretation of circular
intensity differential scattering of chiral aggregates. Biopolymers, 24, 1595
Confidential
Page 83
6/20/2017
Campbell, D., M-J. Eriksson, G. Oquist, P. Gustafsson, & A. K. Clarke. 1998, The
cyanobacerium Synechococcus resists UV-B by exchanging photosystem II reaction-center D1
proteins, Proc. Natl. Acad. Sci. USA. 95:364-369.
Carlson, R.W., Anderson, M.S., Johnson, R.E., Smyth, W.D., Hendrix, A.R., Barth, C.A.,
Soderblom, L.A., Hansen, G.B., McCord, T.B., Dalton, J.B., Clark, R.N., Shirley, J.H., Ocampo,
A.C. & Matson, D.L. 1999, Hydrogen Peroxide on the Surface of Europa. Science 283, 2062
Carlson, R.W., Anderson, M.S., Mehlman, R. & Johnson, R.E. 2005, Distribution of hydrate on
Europa: Further evidence for sulfuric acid hydrate. Icarus, in press.
Chambers, J. E. 1999, A hybrid symplectic integrator that permits close encounters between
massive bodies, MNRAS 304, 793
Champagnon, B., Panczer, G., Chazallon, B., Arnaud, L., Duval, P., Lipenkov, V.Y. 1997,
Nitrogen and Oxygen Guest Molecules in Clathrate Hydrates: Different Sites Revealed by
Raman Spectroscopy. Journal of Raman Spectroscopy 28, 711
Charbonneau, D., Brown, T.M., Noyes, R.W., Gilliland, R.L. 2002, Detection of an extrasolar
planet atmosphere, ApJ, 568, 773
Charbonneaus, D. et al, 2005, Detection of thermal emission from an extrasolar planet, ApJ, 626,
523
Charnley, S.B., Ehrenfreund, P. & Kuan, Y.J. 2001, Spectroscopic diagnostics of organic
chemistry in the protostellar environment. Spectrochim Acta A Mol Biomol Spectrosc. 57, 685
Chyba, C.F. 1993, Explosions of small Spacewatch objects in the Earth's atmosphere. Nature
363, 701
Chyba, C.F. 1997, Life on other moons. Nature 385, 201.
Chyba, C.F. and Hand, K.P. Life without photosynthesis 2001, Science 292, 2026
Chyba, C.F., Hand, K. 2005, Astrobiology: The study of the living universe, ARA&A., 43, 31
Chyba, C. F., Thomas, P. J., Brookshaw, L. & Sagan, C. 1990, Cometary Delivery of Organic
Molecules to the Early Earth, Science, 249, 366
Chyba, C.F., Thomas, P.J. & Zahnle, K. 1993, The 1908 Tunguska explosion: Atmospheric
disruption of a stony asteroid, Nature 361, 40
Cockell, C.S., Catling, D.C., Davis, W.L., Snook, K., Kepner, R.L., Lee, P. & McKay, P. 2000,
The ultraviolet environment of Mars: biological implications past, present and future, Icarus 146,
343
Confidential
Page 84
6/20/2017
Cohen, Y. & Rosenberg, E 1989, Microbial Mats: Physiological Ecology of Benthic Microbial
Communities, American Society for Microbiology, Washington, D.C.
Cooper, J.F., Johnson, R.E., Mauk, B.H., Garrett, H.B. & Gehrels, N. 2001, Energetic Ion and
Electron Irradiation of the Icy Galilean Satellites, Icarus 149, 133
Cronin, J.R & Pizzarello, S. 1997, Enantiomeric excesses in meteoritic amino acids, Science,
275, 951
Cruz, K.L., Reid, I.N., Liebert, J., Kirkpatrick, J.D. &Lowrance, P.J. 2003, Meeting the Cool
Neighbors V: A 2MASS selected sample of ultracool dwarfs, AJ, 126, 3007
DasSarma, S. 2004. Genome sequence of an extremely halophilic archaeon. In Microbial
Genomes. edited by Fraser CM, Read T and Nelson KE Humana Press, Totowa, NJ pp 383-399.
DasSarma, S., Kennedy, S.P., Berquist, B., Ng, W.V., Baliga, N.S., Spudich, J.L., Krebs, M.P.,
Eisen, J.A., Johnson, C.H. & Hood, L. 2001. Genomic perspective on the photobiology of
Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon,
Photosyn. Res. 70, 3
DasSarma, S. & Fleischmann, E.M. 1995. Halophiles. Cold Spring Harbor Laboratory Press,
Plainview, N.Y.
Deming, D., Seager, S., Richardson, L. J. & Harrington, J. 2005, Infrared radiation from an
extrasolar planet, Nature, 434, 740.
DeVeaux et al. 2005, manuscript in submission
DiRuggiero J, Santangelo N, Nackerdien Z, Ravel J & Robb FT. 1997, Repair of extensive
ionizing-radiation DNA damage at 95C in the hyperthermophilic archaeon Pyrococcus furiosus,
J Bacteriol. 179, 4643.
Drennan, C L., Heo, J., Sintchak, M.D., Schreiter, E. & Ludden, P.W. 2001, Life on carbon
monoxide: X-ray strcuture of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase,
Proc. Natl Acad of Sci USA, 98, 11973
Eckburg, P.B., Lepp, P.W., Relman, D.A., 2003, Archaea and their potential role in human
disease, Infection and Immunity, 71, 591
Ehling-Schulz, M. & Scherer, S. 1999, UV protection in cyanobacteria, Eur. J. Phycol. 34, 329.
Ehrenfreund, P. 1999, Molecules on a Space Odyssey, Science, 283, 1123
Faison BD. 2003, Microbiological contributions to the search for extraterrestrial life, Adv Appl
Microbiol. 53, 397
Confidential
Page 85
6/20/2017
Fischer, D.A. & Valenti, J.A. 2005, The planet-metallicity correlation, ApJ, 622, 1102
Fischer, D.A. et al, 2005, The N2K Consortium: I A hot Saturn planet orbiting HD 88133, ApJ,
620, 481
Ford, E. B., Seager, S. & Turner, E. L. 2001, Characterization of extrasolar terrestrial planets
from diurnal photometric variability, Nature, 412, 885.
Formisano, V., Atreya, S., Encrenaz, T., Ignatiev, N. & Giuranna, M. 2004, Detection of
Methane in the Atmosphere of Mars, Science, 306, 1758.
Franzmann, P. D., Y. T. Liu, D. L. Balkwill, H. C. Aldrich, E. C. deMacario & D. R. Boone.
1997, Methanogenium frigidum sp. nov., a psychrophilic, H-2-using methanogen from Ace Lake,
Antarctica, Int J Syst Bact 47, 1068
Franzmann, P. D., N. Springer, W. Ludwig, E. Conway de Macario & M. Rohde. 1992, A
methanogenic archaeon from Ace Lake, Antarctica: Methanococcoides burtonii sp. nov.,
Systematic and Applied Microbiology 15, 573
Franzmann, P. D., Stackebrandt, E., Sanderson, K., Volkman, J. K., Cameron, D. E., Stevenson,
P. L., McMeekin, T. A. & Burton, H. R. 1988, Halobacterium lacusprofundii sp. nov., a
halophilic bacterium isolated from Deep Lake, Antarctica, Syst. Appl. Microbiol, 11, 20
Garab, G. 1996, Linear and circular dichroism. Biophysical Techniques in Photosynthesis, eds. J.
Amesz & A. J. Hoff, Kluwer Academic Publishers, Dordrecht, Netherlands, 11.
Gillard, D.W. & Holdaway, R. 2003, The Astronomical Almanac 2005, Washington: U. S.
Government Printing Office. F14-F37 pp.
Gonzalez, G. 1997, the stellar metallicity-giant planet connection, MNRAS, 285, 403
Haberle, R.M., McKay, C.P., Tyler, D. & Reynold, R. 1994, Can synchronously rotating planets
support an atmosphere?, in Circumstellar Habitable Zones, ed. I. Doyle, Travis House
Publications, Meno Park, CA, p. 29
Hader. D.-P., Kumar, H.D., Smith, S.C. & Worrest, R.C. 1998, Effects of UV-B radiation on
aquatic ecosystems. J. Photochem. Photobiol. B: Biol. 46, 53
Hale, A. 1994, Orbital coplanarity in solar-type binary systems, AJ, 107, 306.
Hall, D. T., Strobel, D. F., Feldman, P. D., McGrath, M. A. & Weaver, H. A. 1995, Detection of
an Oxygen Atmosphere on Jupiter's Moon Europa, Nature, 373, 677.
Hallam SJ, Putnam N, Preston CM, Detter JC, Rokhsar D, Richardson PM & DeLong EF. 2004,
Reverse methanogenesis: testing the hypothesis with environmental genomics, Science 305, 1457
Confidential
Page 86
6/20/2017
Hand, K.P., Carlson, R.W., Sun, H.J., Anderson, M.S., Wadsworth, W. & Levy, R. 2005,
Utilizing active mid-infrared microspectrometry for in-situ analysis of cryptoendolithic microbial
communities of Battleship Promontory, Dry Valleys, Antarctica. Presented at Optics &
Photonics, San Diego, CA.
Hand, K.P., Chyba, C.F., Carlson, R.W. & Cooper, J.F. 2006, Clathrate hydrates of oxidants in
the ice shell of Europa, Asbio submitted
Hartzell PL, Millstein J & LaPaglia C. 1999, Biofilm formation in hyperthermophilic Archaea,
Methods Enzymol. 310, 335
Hawley, S.L., Reid, I.N. ,Gizis, J.E. & Byrne, P.B. 1999, Chromospheric activity in low mass
stars, in Solar and Stellar Activity: Similarities and Differences, ASP Conf. Ser. 158, 63 (ed. C.J.
Butler and J.G. Doyle)
Holman, M. J. & Wisdom, J. 1993, Dynamical stability in the outer solar system and the delivery
of short period comets. AJ, 105, 1987.
Houssier, C. & Sauer, K. 1970, Circular dichroism and magnetic circular dichroism of the
chlorophyll and protochlorophyll pigments, J Amer Chem Soc, 92:4, 779
Hughes, T.R. et al, 2001, Expression profiling using microarrays fabricated by an ink-jet
oligonucleotide synthesizer, Nat Biotechnol 19, 342
Hulshoff Pol LW, de Castro Lopes SI, Lettinga G & Lens PN. 2004, Anaerobic sludge
granulation, Water Res. 38, 1376
Inouye M & Phadtare S. 2004 Cold shock response and adaptation at near-freezing temperature
inmicroorganisms. Sci STKE. 9, 237:pe26.
Javor, BJ. 1989, Hypersaline environments. Microbiology and biogeochemistry, Berlin,
Germany: Springer-Verlag KG
Johnson, R.E., Carlson, R.W., Cooper, J.F., Paranicas, C., Moore, M.H. & Wong, M.C. 2004,
Radiation effects on the surface of the Galilean satellites, In Jupiter, ed. F Bagenal, TE Dowling,
WB McKinnon, pp. 485-512. Cambridge: Cambridge University Press.
Joshi, M., Haberle, R.M. & Reynolds, R.T. 1997, Simulations of the atmospheres of
synchronously rotating terretrial planets orbiting M dwarfs, Icarus, 129, 450
Joshi, M. 2003, Climate model studies ofsynchronously rotang planets, AsBio, 3, 415
Jung, K.-H., Spudich, J. L. 1998, Suppressor Mutation Analysis of the Sensory Rhodopsin ITransducer Complex: Insights into the Color-Sensing Mechanism, J. Bacteriol. 180, 2033
Confidential
Page 87
6/20/2017
Kachlany, S. C., P. J. Planet, M. K. Bhattacharjee, E. Kollia, R. DeSalle, D. H. Fine & D. H.
Figurski. 2000, Nonspecific adherence by Actinobacillus actinoycetemcomitans requires genes
widespread in bacteria and archaea, J. Bacteriol. 182, 6169
Kan, J., Hanson, T.E., Ginter, J.M., Wang, K. & Chen, F. 2005, Metaproteomic analysis of
Chesapeake Bay microbial communities, Saline Systems 1, 7
Kasting, J.F., Whitmire, D.P. & Reynolds, R.T. 1993, Habitable zones around main sequence
stars, Icarus, 101, 108
Kelly, S.M. & Price, N.C. 2000, The use of circular dichroism in the investigation of protein
structure and function. Current Protein and Peptide Science, 1, 349
Kemp, J.C., Wolstencroft, R.D. & Swedlund, J.B. 1971, Circular polarization: Jupiter and other
planets, Nature, 232, 165
Kottemann M, Kish A, Iloanusi C, Bjork S & DiRuggiero J., 2005, Physiological responses of
the halophilic archaeon Halobacterium sp. Strain NRC1 to desiccation and gamma irradiation,
Extremophiles. 9, 219
Kral, T. A., C. R. Bekkum & C. P. McKay. 2004, Growth of methanogens on a mars soil
simulant, Origins of Life and Evolution of the Biosphere 34, 615-626.
Krasnopolsky, V. A., Maillard, J. P. & Owen, T. C. 2004, Detection of methane in the martian
atmosphere: evidence for life? Icarus, 172, 537.
Kruszewski, A. & Semeniuk, I. 2003, A Simple Method of Correcting Magnitudes for the Errors
Introduced by Atmospheric Refraction, AcA, 53, 241.
Kuhs, W.F., Chazallon, B., Radaelli, P.G. & Pauer, F. 1997, Cage Occupancy and
Compressibility of Deuterated N2-Clathrate Hydrate by Neutron Diffraction, Journal of
Inclusion Phenomena and Molecular Recognition in Chemistry 29, 65
Landis, G.A. 2001, Martian Water: Are there extant halobacteria on Mars? Asbio, 1, 161
Lazazzera BA. 2005, Lessons from DNA microarray analysis: the gene expression profile of
biofilms, Curr Opin Microbiol. 8, 222
Leigh, C. Cameron, A. C. & Guillot, T. 2003, Prospects for spectroscopic reflected-light planet
searches, MNRAS, 346, 890.
Lissauer, J. J. 1993, Planet formation, ARA&A, 31, 129
Lopez M. F. 1999, Proteome analysis I. Gene products are where the biological action is, J
Chromatogr. 722, 191
Confidential
Page 88
6/20/2017
McCready, S., Müller, J.A., Boubriak, I., Berquist, B.R., Ng, W.L.& DasSarma, S. 2005, UV
irradiation induces homologous recombination genes in the model archaeon, Halobacterium sp.
NRC-1, Saline Systems 1, 3.
McCullough, P.R., Stys, J.E., Valenti, J.A., Fleming, S.W., Janes, K.A., Heasley, J.N. 2005, The
XO Project: Searching for Transiting Extrasolar Planet Candidates, PASP, 117, 783
McGenity TJ, Gemmell RT, Grant WD & Stan-Lotter H. 2000. Environ Microbiol., 2, 243.
McLaughlin, D. B. 1924, Some results of a spectrographic study of the Algol system, ApJ, 60,
22.
Maira-Litran T, Kropec A, Abeygunawardana C, Joyce J, Mark G 3rd, Goldmann DA & Pier GB
2002, Immunochemical properties of the staphylococcal poly-N-acetylglucosamine surface
polysaccharide, Infect Immun. 70, 4433
Mao, S. & Paczynski, B. 1991, Gravitational microlensing by double stars and planetary
systems, ApJ, 374, L37.
Meierhenrich, U.J., Thiemann, W.H.-P., Barbier, B., Brack, A., Alcaraz, C., Nahon, L. &
Wolstencroft, R. 2002, Circular polarization of light by planet Mercury and enantiomorphism of
its surface minerals, Origins of Life and Evolution of the Biosphere, 32, 181
Melosh, H.J. 2003, Exchange of meteorites (and life?) between stellar systems, AsBio, 3, 207215
Metcalf, W.W., Zhang, J.K., Apolinario, E., Sowers, K.R. & Wolfe, R.S. 1997, A genetic system
for Archaea of the genus Methanosarcina. Liposome-mediated transformation and construction
of shuttle vectors, Proc. Natl. Acad. Sci. USA, 94, 2626
Methe BA et al, 2005, The psychrophilic lifestyle as revealed by the genome sequence of
Colwellia psychrerythraea 34H through genomic and proteomic analyses. Proc Natl Acad Sci U
S A. 102, 10913
Mileikowsky, C. et al 2000. Natural transfer of viable microbes in space: 1. From Mars to Earth
and Earth to Mars, Icarus 145: 391
Moore, M.H. & Hudson, R.L. 2000, IR detection of H2O2 at 80K in ion-irradiated laboratory
ices relevant to Europa, Icarus 145, 282
Müller, J.A. & DasSarma, S. 2005, Genomic analysis of anaerobic respiration in the archaeon
Halobacterium sp. strain NRC-1: dimethyl sulfoxide and trimethylamine N-oxide as terminal
electron acceptors, J Bacteriol. 187, 1659
Mumma, M. J., Novak, R. E., DiSanti, M. A. & Bonev, B. P. 2003, A sensitive search for
methane on Mars, DPS, 35, 1418
Confidential
Page 89
6/20/2017
Narita, N., Suto, Y., Winn, J. N., Turner, E. L., Aoki, W., Leigh, C. J., Sato, B., Tamura, M. &
Yamada, T. 2005, Subaru HDS Transmission Spectroscopy of the Transiting Extrasolar Planet
HD 209458b, Pub. Astron. Soc. Japan, 57, 471.
National Research Council 2005, Preventing the Forward Contamination of Mars (Washington
DC: National Academies Press), www.nap.edu
Nidever, D.L., Marcy, G.W., Butler, R.P., Fischer, D.A. & Vogt, S.S. 2002, Radial velocities for
889 late-type stars, ApJS, 141, 503
Nordstrom, B. et al. 2004, The Geneva-Copenhagen survey of the Solar neighbourhood. Ages,
metallicities, and kinematic properties of ~14 000 F and G dwarfs, A&A, 418, 989
Ohta, Y., Taruya, A. & Suto, Y. 2005, The Rossiter-McLaughlin Effect and Analytic Radial
Velocity Curves for Transiting Extrasolar Planetary Systems, ApJ, 622, 1118.
Orphan VJ, House CH, Hinrichs KU, McKeegan KD & DeLong EF. 2001, Methane-consuming
archaea revealed by directly coupled isotopic and phylogenetic analysis, Science, 293, 484
Paczynski, B. 1986, Gravitational microlensing at large optical depth, ApJ, 301, 503
Paranicas, C., Carlson, R.W. & Johnson, R.E. 2001, Electron bombardment of Europa, Geophys.
Res. Lett. 28, 673
Pierazzo, E., Chyba, C.F., 2000, Impact delivery of organics to Europa, LPI, 31, 1656
Pizzarello, S. & Cronin, J.R 2000, Non-racemic amino acids in the Murray and Murchison
meteorites, Geochim. Cosmochim. Acta, 64, 329
Pospergelis, M.M. 1969, Spectroscopic measurements of the four Stokes parameters for light
scattered by natural objects, Soviet Physics - Astronomy, 12, 973
Purdie, N. & Brittain, H.G. 1993, Analytical Applications of Circular Dichroism (Techniques &
Instrumentation in Analytical Chemistry), 14, Elsevier
Pysz, M.A. et al, 2004, Transcriptional analysis of biofilm formation processes in the anaerobic,
hyperthermophilic bacterium Thermatoga maritima, Appl. Environ. Microbiol., 70, 6098
Orphan VJ, House CH, Hinrichs KU, McKeegan KD & DeLong EF. 2001, Methane-consuming
archaea revealed by directly coupled isotopic and phylogenetic analysis, Science, 293, 484
Queloz, D., Eggenberger, A., Mayor, M., Perrier, C., Beuzit, J. L., Naef, D., Sivan, J. P. & Udry,
S. 2000, Detection of a spectroscopic transit by the planet orbiting the star HD209458, A&A,
359, L13.
Confidential
Page 90
6/20/2017
Rafikov, R. R. 2001, Slowing down Planetary Growth by Scattering of Planetesimals, AJ, 122,
2713
Ram R. J., VerBerkmoes, N.C., Thelen, M.P., Tyson, G.W., Baker, B.J., Blake II, R.C., Shah,
M., Hettich, R.L. & Banfield, J.F. 2005, Community proteomics of a natural microbial biofilm,
Science, 308, 1915
Reid, I.N. 2002, How unusual are stars with planets? PASP, 114, 306
Reid, I.N. 2006, Extrasolar planets: a Galactic perspective, in A Decade of Extrasolar Planets,
2005 STScI May Symposium, ed. M. Livio, Cambridge Univ. Press
Reid, I.N. & Cruz, K.L. 2001, Meeting the Cool Neighbors I: Nearby stars in the NLTT
catalogue, AJ, 123, 2806
Reid, I.N. et al. 2004 Meeting the Cool Neighbors VIII: A preliminary 20-parsec census from the
NLTT catalogue, AJ, 128, 463
Reid, I.N., Gizis, J.E. & Hawley, S.L. 2002, The PMSU survey IV: The luminosity function in
the Solar neighborhood and M dwarf kinematics, AJ, 1284 2721
Reid, I.N., W.B. Sparks, S. Lubow, M. McGrath, M. Livio, J. Valenti, K.R. Sowers, H.D.
Shukla, S. MacAuley, T. Miller, R. Suvanasuthi, R. Belas, A. Colman , F.T. Robb, P. DasSarma,
J.A. Müller, J.A. Coker, R. Cavicchioli, F. Chen & S. DasSarma. 2006, Terrestrial models for
extraterrestrial life: methanogens and halophiles at Martian temperatures, Asbio (submitted).
Resch A, Rosenstein R, Nerz C & Gotz F. 2005, Differential gene expression profiling of
Staphylococcus aureus cultivated under biofilm and planktonic conditions, Appl Environ
Microbiol. 71, 2663
Rieke, G. H., et al. 2005, decay of planetary debris disks, ApJ, 620, 1010
Rinker KD & Kelly RM. 2000, Effect of carbon and nitrogen sources on growth dynamics and
exopolysaccharide production for the hyperthermophilic archaeon Thermococcus litoralis and
bacterium Thermotoga maritima.Biotechnol Bioeng. 69, 537
Rossiter, R. A. 1924, On the detection of an effect of rotation during eclipse in the velocity of the
brighter component of beta Lyrae, ApJ, 60, 15.
Rothschild, L. J. & R. L. Mancinelli. 2001, Life in extreme environments, Nature 409, 1092
Rothschild, L. J. 2002, Life in extreme environments: The universe may be more habitable than
we thought: June issue of the National Space Society's Magazine Ad Astra
(http://www.spaceref.com/news/viewnews.html).
Confidential
Page 91
6/20/2017
Saffary R, Nandakumar R, Spencer D, Robb FT, Davila JM, Swartz M, Ofman L, Thomas RJ &
DiRuggiero J. 2002, Microbial survival of space vacuum and extreme ultraviolet irradiation:
strain isolation and analysis during a rocket flight, FEMS Microbiol Lett. 24, 163
Salzman, G.C. & Gregg, C.T. 1984, Current and experimental methods of rapid microbial
identification, Biotechnology, 2, pp 243
Sanford, S.A. 2005, Organic synthesis in space, in The Astrophysics of Life (2002 STScI May
Symposium), ed. M. Livio, I.N. Reid and W.B. Sparks, pp 54-66
Santos, N.C. et al, 2004, The HARPS survey for southern extra-solar planets. II. A 14 Earthmasses exoplanet around mu Arae, A&A, 426, L19
Saunders, N.F.W., Thomas, T., Curmi, P.M.G., Mattick, J.S., Kuczek, E., Slade, R., Davis, J.,
Franzmann, P.D., Boone, D., Rusterholtz, K., Feldman, R., Gates, C., Bench, S., Sowers, K.,
Kadner, K., Aerts, A., Dehal, P., Detter, C., Glavina, T., Lucas, S., Richardson, P., Larimer, F.,
Hauser, L., Land, M. & Cavicchioli, R. 2003, Mechanisms of thermal adaptation revealed from
the genomes of the Antarctic Archaea Methanogenium frigidum and Methanococcoides burtonii,
Genome Research, 13, 1580
Schneider, J. 2005, in The Extrasolar Planets Encyclopedia,
http://vo.obspm.fr/exoplanetes/encyclo/encycl.html .
Schopf, J. W.1993, Microfossils of the Early Archean Apex chert: New evidence of the antiquity
of life, Science 260, 640
Seager, S., Ford, E. B. & Turner, E. L. 2002, Characterizing Earth-like planets with terrestrial
planet finder, Proc SPIE, 4835, 79.
Seager, S., Turner, E.L., Schafer, J. & Ford, E.B. 2005, Vegetation’s red edge: a possible
spectroscopic biosignature, AsBio, 5, 372
Sparks, W.B., Hough, J.H. & Bergeron, L. 2005, A search for chiral signatures on Mars, Asbio,
5, in press
Spear, J.R., Ley, R.E., Berger, A.B., Pace, N.R., 2003, The archae of a hypersaline microbial
mat, Biol. Bull., 204, 168
Spencer, J.R. & Calvin, W.M. 2002, Condensed O2 on Europa and Callisto. AJ, 124, 3400
Spohn, T. & Schubert, G. 2003, Oceans in the icy Galilean satellites of Jupiter. Icarus, 161, 456
Sullivan MB, Coleman ML, Weigele P, Rohwer F & Chisholm SW 2005, Three
Prochlorococcus Cyanophage Genomes: Signature Features and Ecological Interpretations, PLoS
Biol 3, e144
Confidential
Page 92
6/20/2017
Swedlund, J.B., Kemp, J.C., Wolstencroft, R.D. 1973, Circular polarization of Saturn, ApJ, 178,
257
Tamuz, O., Mazeh, T. & Zucker, S. 2005, Correcting systematic effects in a large set of
photometric light curves, MNRAS, 356, 1466
Tanaka, H., Takeuchi, T. & Ward, W. R. 2002, Three-Dimensional Interaction between a Planet
and an Isothermal Gaseous Disk. I. Corotation and Lindblad Torques and Planet Migration, ApJ,
565, 1257.
Thomas, N. A., S. L. Bardy, & K. F. Jarrell. 2001, The archaeal flagellum: a different kind of
prokaryotic motility structure, FEMS Microbiol. Rev. 25, 147
Thomas, T., N. Kumar & R. Cavicchioli. 2001, Effects of ribosomes and intracellular solutes on
activities and stabilities of elongation factor 2 proteins from psychrotolerant and thermophilic
methanogens, J. Bacteriol. 183, 1974
Thieringer H.A., Jones, P.G. & Inouye, M. 1998, Cold shock and adaptation, Bioessays, 1, 4957.
Tinnetti, G., Meadows, V.S., Crisp, D., Kiang, N., Kahn, B.G., Bose, E., Fishbein, E., Velusamy,
T., & Turnbull, M.C., 2005, Detectability of Planetary Characteristics in Disk-averaged Spectra
II: Synthetic Spectra and Light-curves of Earth, AsBio, subm.
Turnbull, M.C. & Tarter, J.C. 2003a, Target Selections for SETI I. A Catalog of Nearby
Habitable Systems, ApJS, 145, 181
Turnbull, M.C. & Tarter, J.C. 2003b, Target Selections for SETI II. Tycho-2 Dwarfs, Old Open
Clusters and the Nearest 100 Stars, ApJS, 149, 423
Turnbull, M.C., Traub, W.A., Juck, K.W., Gorlova, N., Wilson, J. & Skrutskie, M., 2005,
Spectrum of a Habitable World: Earthshine in the Near-Infrared ApJ, in prep.
Turtle, E.P. & Pierazzo, E. 2001, Thickness of a Europan Ice Shell from Impact Crater
Simulations. Science, 294, 1326
Tyson GW, Chapman J, Hugenholtz P, Allen EE, Ram RJ, Richardson PM, Solovyev VV, Rubin
EM, Rokhsar DS & Banfield JF. 2004, Community structure and metabolism through
reconstruction of microbial genomes from the environment, Nature, 428, 37
Udalski, A. 2003, The Optical Gravitational Lensing Experiment. Real Time Data Analysis
Systems in the OGLE-III Survey, AcA, 53, 291.
Udalski, A., Jaroszynski, M., Paczynski, B. at al. 2005, A Jovian-Mass Planet in Microlensing
Event OGLE-2005-BLG-071, ApJ, 628, L109.
Confidential
Page 93
6/20/2017
Valenti, J.A. & Fischer, D.A. 2005, Spectroscopic properties of cool stars I: 1040 FGK dwarfs
from the Keck, Lick and AAT Planet searches, ApJS, 159, 141
Vass, I., D. Kirilovsky & A. L. Etienne. 1999, UV-B radiation-induced donor- and acceptor-side
modifications of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803,
Biochemistry 38, 12780
Vass, I., D. Kirilovsky, 1. Perewoska, Z. Mate, F. Nagy & A. L. Etienne. 2000, UV-B radiation
induced exchange of the D1 reaction centre subunits produced from the psbA2 and psbA3 genes
in the cyanobacterium Synechocystis sp. PCC 6803, Eur. J. Biochem. 267, 2640
Vidal, R.A., Bahr, D., Baragiola, R.A. & Peters, M. 1997, Oxygen on Ganymede: Laboratory
studies, Science 276, 1839
Vidal-Madjar, A., Lecavelier des Etangs, A., Desert, J.-M., Ballester, G. E., Ferlet, R., Hebrard,
G. & Major, M. 2003, An extended upper atmosphere around the extrasolar planet HD 209458b,
Nature, 422, 143.
Vidal-Madjar, A. et al 2004, Deection of oxygen and carbon in the hydrodynamically escaping
atmosphere of the extrasolar planet HD 209458b, ApJ, 604, L79
Vreeland RH, Rosenzweig WD & Powers DW. 2000, Isolation of a 250 million-year-old
halotolerant bacterium from a primary salt crystal, Nature 407, 897
Vuong C, Kocianova S, Voyich JM, Yao Y, Fischer ER, DeLeo FR & Otto M. 2004, A crucial
role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and
virulence. J Biol Chem. 279(52), 54881
Wang, X. & B. Seed. 2003, Selection of oligonucleotide probes for protein coding sequences,
Bioinformatics 7, 796
Watson, J. A., Donahue, T. M. & Walker, J. C. G. 1981, The dynamics of a rapidly escaping
atmosphere – applications to the evolution of Earth and Venus, Icarus, 48, 150.
Weiss BP, Yung YL, Nealson KH. 2000, Atmospheric energy for subsurface life on Mars? Proc
Natl Acad Sci U S A. 97:1395.
Whitton, B. A. & M. Potts 2000, Introduction to the Cyanobacteria. pp. 1-11. In: The Ecology of
Cyanobacteria: Their Diversity in Time and Space. Ed. B. A. Whitton and M. Potts. Kluwer
Academic Publishers, Dordrecht, The Netherlands.
Wickramasinghe, R. H. 1976, Model role for bacteriorhodopsin for solar energy utilization by
primordial organisms, Cytobios. 17, 31
Confidential
Page 94
6/20/2017
Winebrenner, D.P. & Ashner, W.E. 2000, Toward remote detection of life based on circular
polarization differential scattering at optical wavelengths, First Astrobiology Science
Conference, NASA Ames
Winn, J. N., Noyes, R. W., Holman, M. J., Charbonneau, D., Ohta, Y., Taruya, A., Suto, Y.,
Narita, N., Turner, E. L., Johnson, J. A., Marcy, G. W., Butler, R. P. & Vogt, S. S. 2005,
Measurement of spin-orbit alignment in an extrasolar planetary system, ApJ, 631, 1215.
Winn, J. N., Suto, Y., Turner, E. L., Narita, N., Frye, B. L., Aoki, W., Sato, B. & Yamada, T.
2004, A
Pub.
Astron. Soc. Japan, 56, 655.
Woese CR 1987, Bacterial evolution, Microbiol Rev. 51(2), 221
Woese CR & Fox GE. 1977, Phylogenetic structure of the prokaryotic domain: the primary
kingdoms. Proc Natl Acad Sci U S A. 74(11), 5088
Wolstencroft, R. 1974, The circular polarization of light reflected from certain optically active
surfaces, Planets, Stars, and Nebulae: Studied with Photopolarimetry, (Ed Gehrels. University
of Arizona Press, 1974), 495
Wolstencroft, R.D. & Raven, J.A. 2002, Photosynthesis: likelihood of occurrence and possibility
of detection on Earth-like planets, Icarus, 157, 535
Wolstencroft, R., Tranter, G. & D. D. Le Pevelen 2002, Diffuse reflectance circular dichroism
for the detection of molecular chirality: an application in remote sensing of flora. Bioastronomy
2002: Life Among the Stars, I.A.U. Symposium 213, eds. R. Norris & F. Stootman; ASP, 149
Woolf, N.J. & Angel, R.J. 1998, Astronomical Searches for Earth-Like Planets and Signs of Life,
ARA&A, 36, 507
Woolf, N. J., Smith, P. S., Traub, W. A. & Jucks, K. W. 2002, The Spectrum of Earthshine: A
pale blue dot observed from the ground. ApJ, 574, 430.
Wouters JA, Frenkiel H, de Vos WM, Kuipers OP & Abee T. 2001, Cold shock proteins of
Lactococcus lactis MG1363 are involved in cryoprotection and in the production of cold-induced
proteins, Appl Environ Microbiol. 67, 5171
Wu, M, Qinghu Ren, Durkin, A.S., Daugherty, S.C., Brinkac, L.M., Dodson, R.J., Gonzalez,
J.M., Zhulin, I.B., Robb, F.T. & Eisen, J.A 2005, Life in Hot Carbon Monoxide: the Complete
Genome Sequence of Carboxydothermus hydrogenoformans Z-2901 PLos Genetics, in press
Wynn-Williams, D.D. 2000, Cyanobacteria in Dessert – Life at the Limit? pp. 341-366. In: The
Ecology of Cyanobacteria: Their Diversity in Time and Space. Ed. B. A. Whitton and M. Potts.
Kluwer Academic Publishers, Dordrecht, The Netherland
Confidential
Page 95
6/20/2017
Zhang T, Fang HH & Ko BC. 2003, Methanogen population in a marine biofilm corrosive to
mild steel, Appl Microbiol Biotechnol. 63, 101
Zuckerman, B. & Song, I. 2004, Young stars near the Sun, ARA&A, 42, 685
9.2 E/PO references
Christian, C.A. 2004, Creation of Educational Resources: A Research Scientist's Role,
BioAstronomy: Life Among the Stars, IAU Symposium, 213, 545.
Christian, C.A., Eisenhamer, B., Kakadelis, S. & Steiner, M. 2001, NPR Radio & Web
Simulcasts: The Tour the Cosmos Series, WebNet Journal (currently E-Learning), 3, 30.
Frederick, J.A., Jacobs, D. & Jones, W.R. 2000, Biofilms and Biodiversity: an interactive
exploration of aquatic microbial biotechnology and ecology, Journal of Industrial Microbiology
and Biotechnology 24, 334.
Frederick, J. A. and Takacs, J. 2005, Chesapeake Teacher Research Fellowship: opportunities for
teachers in the mid-Atlantic, presentation at Conference on Teacher Research Experiences,
Rhode Island University, Sponsored by NSF.
Confidential
Page 96
6/20/2017
10. Appendix: Glossary of terms and acronyms
2MASS
AAS
ACS
ACP
AMASE
AO
APO
AU
CCD
CIDS
CIW
COEL
COMB
CPD
CSD
CSP
CTIO
DMSO
DNA
DOE
EATT
E/PO
ESA
ESO
ExSUF
FISH
Gaia
Gemini
GMT
GSFC
Gyr
HiCIAO
Hipparcos
HST
IAC
JGI
JWST
KPNO
LINAC
MARTE
MAST
Myr
Confidential
2-micron All-Sky Survey, near-infrared sky survey
American Astronomical Society
Advanced Camera for Surveys (a CCD imaging camera on HST)
Astrobiology Certification program (at PU)
Arctic Mars Analog Svalbard Expedition
Adaptive Optics (astronomical technique for image sharpening)
Apache Point Observatory
Astronomical Unit, the Earth-Sun distance
Charge-coupled device
Circular intensity differential scattering
Carnegie Institution of Washington
Committee on the Origin and Evolution of Life (National Academy of Sciences)
Center of Marine Biotechnology
cyclobutane pyrimidine dimers
cold shock domain
cold shock proteins
Cerro-Tololo Interamerican Observatory, Chile
Dimethyl sulphoxide
Deoxyribonucleic acid
Department of Energy
Extremophiles in Astrobiology: Theory and Techniques
Education/Public Outreach
European Space Agency
European Southern Observatory
Extreomphile scale-up facility
Fluorescence in situ hybridization
ESA astrometric mission, scheduled for launch in 2015
US 8-metre class telescopes on Mauna Kea, Hawaii, and Cerro Pachon, Chile
Greenwich Mean Time
Goddard Space Flight Center
Gigayear
High Contrast Coronagraphic Imager with Adaptive Optics
ESA satellite that measured accurate positions, motions and distances for
118,000 stars, including many in the Solar Neighborhood
The Hubble Space Telescope
Idaho Acceleration Center, Idaho State University
Joint Genome Institute
The James Webb Space Telescope
Kitt Peak National Observatory
Linear accelerator at the IAC
Mars Analog Research and Technology Experiment
Multimission Archive at STScI: an archive of data from several space missions
Megayear
Page 97
6/20/2017
NAI
NAOJ
NASA
NIC-FIPS
NIST
NOAA
NSF
NVO
OGLE
OPO
ORF
NASA Astrobiology Institute
National Astronomical Observatory of Japan
National Aeronatics and Space Administration
Near-infrared camera – Fabry-Perot Spectrograph (at APO)
National Institute of Standards and Technology
National Oceanic and Atmospheric Administration
National Science Foundation
National Virtual Observatory
Optical Gravitational LEns survey
Office of Puiblic Outreach, STScI
Open Reading Frame, the DNA or RNA sequence between the start and stop
codon sequences
parsec
Astronomical distance unit, corresponding to 3.26 light years or 206265 AU
PCR
Polymerase chain reaction
PEM
Photo-elastic modulator
PEMS
Portable Earthsine Monitoring System
PU
Princeton University
RNA
Ribonucleic acid
rRNA
recombinant RNA
RSS
really simple syndicate
SAO
Smithsonian Astrophysical Observatory
SIMBAD
An astronomical database maintained at the University of Strasbourg
SIM Planetquest The Space Interferometry Mission, a NASA mission designed to obtain
high precision positions and detect planetary companions to nearby stars
SMARTS
Consortium of universities and research institutions that run the smaller
telescopes at CTIO
SNH Census
Solar Neighborhood Habitability Census
STIS
Space Telescope Imaging Spectrograph
STScI
Space Telescope Science Institute
TMAO
Trimethylamine N-oxide
TPF
The Terrestrial Planet Finder
TPF-C
The Terrestrial Planet Finder: Coronagraph
TPF-I
The Terrestrial Planet Finder: Interferometer
Tycho
Auxiliary star catalogue produced by the Hipparcos satellite
UCLA
University of California at Los Angeles
UMBI
University of Maryland Biotechnology Institute
UNSW
University of New South Wales
VLT
Very Large Telescope (Four 8-metre telescopes at the ESO Paranal site, Chile)
VPL
Virtual Planetary Laboratory (http://vpl.ipac.caltech.edu/)
VRML
Virtual reality markup language
Confidential
Page 98
6/20/2017