Download biology 160 laboratory objectives practical one

BIOLOGY 160 LABORATORY OBJECTIVES PRACTICAL TWO
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Know that cells use ATP as their energy source; which comes from chemical reactions.
Know the types of nutrients heterotrophs can use for nutrition.
Know the name of the process by which glucose is converted into ATP.
Know the three steps of cellular respiration; know where in the cell each takes place; know which produces the most
ATP and how much.
Know which part of cellular respiration was studied in lab and the reaction that results and the names of the
chemicals involved. Know which is oxidized and which is reduced in the process.
Know the role of DPIP in the cellular respiration lab. Know its color when oxidized and when reduced.
Know what a spectrophotometer is, when we used it in lab and what it tells us.
Know what absorbance means and it relationship to the cellular respiration experiment and results.
Know the results of the cellular respiration experiment and what they mean.
Know that yeast use alcoholic fermentation to make glucose; know this is a type of glycolysis and is the first step of
aerobic or cellular respiration.
Know the name of the yeast used in the fermentation experiment.
Know the products of alcoholic fermentation.
Be able to explain the set-up of the alcoholic fermentation and the cellular respiration experiments.
Be able to explain the results of both the cellular respiration and the fermentation experiments.
Know all the stages of mitosis and meiosis and be able to draw each.
Be able to identify the stages of mitosis in the onion root tip and the white fish blastula and identify that these were
used in the lab.
Know what meristematic tissue refers to and where it can be found.
Be able to identify the root cap.
Be able to identify the number of cells made in meiosis and mitosis.
Be able to explain haploid and diploid and how it relates to meiosis and mitosis.
Be able to identify spindle fibers and cleavage furrows.
Know what a chiasmata is and when it occurs.
Know the importance of crossing over.
Know how to calculate the amount of time cells are in a specific phase of mitosis and how to do the equation
discussed in lab on time in interphase. Know that interphase is the longest phase of the cell cycle and prophase is the
longest phase of mitosis.
Be able to explain and solve genetics problems as discussed in lab and in class. Know how to read and analysis a
pedigree.
Be able identify a trait as dominant or recessive and know what this means and how many genes are needed to
express each.
Be able to explain geneotype and phenotype and give an example of each.
Know what codominance, dominance and incomplete dominance mean.
Know what homozygous and heterozygous mean
Know what a monohydrid and a dihydrid cross are and know how to show on a Punnett square
Know how DNA is replicated.
Know how DNA bases match.
Know what ACTG stand for.
Know how to use a micropipettor. Know the difference between the ones used in lab.
Know what not to do with a micropipettor. Know the best practices of using a micropipettor.
Know how you determined how well you pipetted with the micropipettors.
Know what a DNA restriction enzyme is. Be able to identify ones used in lab. Know the purpose of using a restriction
enzyme.
Be able to explain the experiments conducted in the DNA lab.
Know the results of the DNA experiment.
Know what gel electrophoresis is and the result of your electrophoresis of DNA.
Know the function of the loading dye and what it is composed of and why the ingredients are used.
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Know what happens if the voltage is increased during electrophoresis
Know what happens if the agar is not poured evenly.
Know that agar is and what it was used for in lab.
Know what the microcentrifuge was used for in the lab.
Know that happens if the electrodes are reversed in electrophoresis.
Know why buffer was used in the DNA lab.
Know what happens if there is too much DNA loaded onto a gel.
Be able to use a dissecting and compound microscope and know when each is to be used.
Know the parts of each of the organisms reviewed in the lab: sponge, hydra, planarian, grasshopper, daphnia, and
rotifer. Know the phylum to which each belongs
51. Know what happens when light is shined on a planarian. Know what the organism needs to be able to detect light; know
the name of this reaction.
52. Know what happened when the organism was poked with a probe.
53. Know which organisms had a body cavity and which did not. Know the symmetry of each organism.