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Circuit-Tracing at Neuro-Electronics Research Flanders Amy Kristl, Department of Biology, Northeastern University, Boston MA Introduction Outcomes About Leuven About NERF Research Founded by IMEC, KU Leuven, and VIB with funding from Flemish Government Home of KU Leuven, the world’s oldest Catholic university Boasts the “longest bar” Interdisciplinary research initiative with the goal of understanding the brain and its function world’s • • Home of the original Stella Artois brewery Belgian waffles Injections into the LP and superior colliculus reliably labeled neurons in the superior colliculus and retina, respectively. This virus will be used to guide future optogenetics and in vivo imaging experiments. Currently hosts five laboratories Science frequently discussed over coffee at JAVA About the Farrow Lab Retina 5x Personal The lab studies the retina and downstream brain areas involved in innate visuallyguided behaviors through a computational lens that aims to understand the brain’s computational power. • Refined skills in histology and surgery • Discovered interests in computational neuroscience and biotechnology • Adapted to an entirely new culture • Travelled to ~20 cities across Europe! Activities • • • • Stereotaxic viral injections into the pulvinar, superior colliculus Histology: Perfusions, slicing, immunostaining, slide preparation Confocal imaging to observe labeling Training mice for other experiments Acknowledgements Special thanks to my supervisor Karl Farrow, fellow co-op Leighanne Wang, and the Farrow lab for their support during this co-op; Northeastern University and KU Leuven for funding; Naomi ZingmanDaniels and Logan Griffiths for poster assistance. Retina 40x