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Transcript
Integrated
tegrated active PCR chips - 253
Ground-loop check
A simple experiment was devised to check out ground-loop effects. The
sensing and drive circuitries were physically disconnected, and the driver
was stimulated manually by direct connection to the driver power supply.
Results (see Figure 146) proved beyond reasonable doubt that the nonlinearity observed in polysilicon resistance measurements was due to
ground-loop effects, and a serious redrawing of the underlying circuitry was
undertaken.
PCR Cycles
Pt100 temperature
120
Polysilicon resistance
Linear interpolation
110
Temperature (ºC)
100
90
80
70
60
50
40
30
0
20
40
Time (s)
60
Figure 146 - Temperature sensing with physically isolated ground sources. Polysilicon
sensor output becomes again manifestly linear (as demonstrated by the linear fit with
Pt100 read temperature (green line)) and thus calls for discarding both infrared radiation
and capacitive coupling hypotheses in favor of a simpler ground-loop explanation.
Circuitry reconfiguration
Although ground loops provide a far simpler account of sensor non-linearity
than either infrared or capacitive coupling, this does not imply that
circumventing them is a simple issue. In fact, dealing with ground loops is
an extremely tricky design issue in power electronics and is often found to
be at the core of many unapparent design dysfunctions. In this particular
case, the elimination of ground loops required a careful redesign of the
driver circuitry and, perhaps, of some parts of the sensing circuitry.
Moreover, since ground-loop effects had not been observed during
calibration and direct coupling factors had been already dismissed, the
obvious conclusion was that the own heater resistor grid (a 5-6 Ω resistor)
254
4 - Active PCR-chips
was closing a relatively mild ground path, and circumventing this problem
could ask for to very elaborate ground control mechanisms. Therefore,
considering the foreseeable amount of time that such a circuitry redesign
and adjustment would take, a more drastic alternative (photoelectric
ground isolation) was taken for practical reasons, even thought this
solution was not extremely elegant in an electronic sense.
Figure 147 - Schematic circuit for photoelectric isolation of the PC-DAC drive output.
Green lines represent the only possible current flows. A similar circuit was developed for
sensing of driver power consumption voltage outputs.
Effective photoelectric isolation between sensing and driving circuitries was
accomplished by means of a HCNR200 high-linearity analog opto-coupler
(Agilent Technologies). In contrast to conventional, digital opto-couplers, the
HCRN200 exhibits two separate photodiode (instead of transistor-like)
outputs. A light-emitting photodiode (LED) is placed in between, delivering
approximately the same amount of light to both receiving photodiodes. An
external OP07 amplifier (Analog Devices) monitors the LED output light
through a negative feedback link using the first photodiode, and thus
stabilizes the LED output, which is in turn regulated by the input voltage.
The output photodiode then converts the stable, linear light output of the
LED back into current, and a second OP07 amplifier reverts this current to
voltage. Since op-amps tightly limit current flow, the following assumptions
can be made on the circuit:
Ii =
Vi
;
100kΩ
I i = k ·I o ;
Vo = I o ·100kΩ ; ⇒ Vo =
Vi
k
Equation 12 - Basic equations governing Figure 147 circuit. k is a linear gain constant
provided by the opto-coupler manufacturer.
Integrated
tegrated active PCR chips - 255
Therefore, by thoroughly adjusting both feedback resistors, a linear relation
between input and output voltages can be attained. Additionally, the 1.5 kΩ
load resistor is used to limit the maximum current that could flow through
the LED (~15 mA) and thus avoid LED damage. After the incorporation of
the opto-coupler isolation boards, the resulting complete circuitry setup
was as shown in Figure 148.
Figure 148 - Circuit interconnection diagram for photo-electrically insulated sensing and
driving circuitries.
Initial functional tests
Sensor-actuator test
Using the previously calibration-derived (see p.251) third-order polynomial
interpolations and the new circuitry setup, a preliminary test of active PCRchips working in dual (sensor-actuator) mode was carried out with the
clamped Pt100 sensor as an external control check. The plot in Figure 149
demonstrated the validity of the ground isolation circuitry setup, and
prompted to the first functional PCR tests using active chips as fully
independent devices.