Download : BIOGRAPHICAL SKETCH

Principal Investigator/Program Director (Last, First, Middle):
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Jihyeon Lim
Assistant Professor
eRA COMMONS USER NAME
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
Korea University
Korea University
University of Massachusetts at Amherst
Albert Einstein College of Medicine
DEGREE
(if applicable)
YEAR(s)
BS
MS
PhD
Postdoc
1991-1995
1995-1997
1999-2004
2004-2005
FIELD OF STUDY
Chemistry
Inorganic Chemistry
Analytical Chemistry
BioAnalytical Chemistry
Positions and Honors
1995-1997
Research Assistant, Korea Basic Science Institute, Seoul branch, Korea
1997-1998
Research Scientist, Product Research Department, Hanwha Energy Co., Ltd., Daejon,
South Korea
1999-2004
Research Assistant (Ph.D. Candidate), University of Massachusetts at Amherst, MA, NY
2004–2005
Research Associate, Department of Molecular Pharmacology, Albert Einstein College of
Medicine at Bronx, NY
2005-2007
Associate, Laboratory for Macromolecular Analysis & Proteomics
Albert Einstein College of Medicine at Bronx, NY
2007-present Assistant Professor, Department of Pathology,
Albert Einstein College of Medicine at Bronx, NY
Professional service
2006-present Reviewer, Analytical Biochemistry
Memberships:
American Chemical Society, American Society for Mass Spectrometry
Publications
1. Du, P. C.; Stolovitzky, G.; Horvatovich, P.; Bischoff, R.; Lim, J.; Suits, F. “A noise model for mass
spectrometry based proteomics” Bioinformatics 2008, 24 (8), 1070-1077.
2. Che, F.Y; Zhang, X.; Berezniuk I.; Callaway, M.; Lim, J; Fricker, L.D. “Optimization of neuropeptide
extraction from the mouse hypothalamus” J. Proteome Res. 2007, 6(12), 4667-4676.
3. Srikanth, R.; Wilson, J; Bridgewater, J. D.; Numbers, J. R.; Lim, J.; Olbris, M.R.; Kettani,A.; Vachet,
R.W. “Improved Sequencing of Oxidized Cysteine and Methionine Containing Peptides Using Electron
Transfer Dissociation” J. Am. Soc. Mass Spectrom. 2007, 18(8),1499-506.
4. Bridgewater, J.D.; Srikhanth, R.; Lim, J.; Vachet, R.W. “The Effect of Histidine Oxidation on the
Dissociation Patterns of Peptide Ions” J. Am. Soc. Mass Spectrom. 2007, 18(3), 553-562.
5. Bridgewater, J.D.; Lim, J.; Vachet, R.W. “Using Metal-Catalyzed Oxidation Reactions and Mass
Spectrometry to Identify Amino Acid Residues within 10Å of the Metal Center in Cu-Binding Proteins” J.
Am. Soc. Mass Spectrom. 2006, 17(11), 1552-1559.
6. Bridgewater, J.D.; Lim, J.; Vachet, R.W. “Transition Metal-Peptide Binding Studied by Metal-Catalyzed
Oxidation Reactions and Mass Spectrometry” Anal. Chem. 2006, 78(7), 2432-2438.
7. Lim, J.; Berezniuk, I.; Che, F.; Parikh, R.; Biswas, R.; Pan, H.; Fricker, L.D. “Altered Neuropeptide
Processing in Prefrontal Cortex of Cpe (fat/fat) Mice: Implications for Neuropeptide Discovery” J.
Neurochem. 2006, 96(4), 1169-81.
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
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Principal Investigator/Program Director (Last, First, Middle):
8. Fricker, L.D.; Lim, J.; Pan, H.; Che, F. “Peptidomics: Identification and Quantification of Endogenous
Peptides in Neuroendocrine Tissues” Mass Spectrom. Rev. 2006, 25(2), 327-44.
9. Che, F.; Lim, J.; Biswas, R.; Pan, H; Fricker, L.D. “Quantitative Neuropeptidomics of Microwaveirradiated Mouse Brain and Pituitary” Mol. Cell. Proteomics 2005, 4(9),1391-405.
10. Lim, J.; Vachet, R. W. “Using Mass Spectrometry To Study Copper-Protein Binding under Native and
Non-Native Conditions: -2-Microglobulin” Anal. Chem. 2004, 76(13), 3498-3504.
11. Lim, J.; Vachet, R. W. “Development of a Methodology Based on Metal-Catalyzed Oxidation Reactions
and Mass Spectrometry to Determine the Metal Binding Sites in Copper Metalloproteins” Anal. Chem.
2003, 75(5), 1164-1172.
12. Lim, J. H.; Park, T. H.; Lee, H. J.; Lee, K. B.; Jang, H. G. “A New Functional Model Complex of
Extradiol-Cleaving Catechol Dioxygenases: Properties and Reactivity of [Fe-II(BLPA)DBCH] BPh4” B.
KOR. CHEM. SOC. 1999, 20 (12), 1428-1432.
13. Lim, J. H.; Lee, H. J.; Lee, K. B.; Jang, H. G. “A New Functional Model of Catechol Dioxygenases:
Properties and Reactivity of [Fe(BLPA)DBC]BPh4 ” B. KOR. CHEM. SOC. 1997, 18 (11), 1166-1172.
Research Support
ƒ Recently, Dr. Lim’s research is more focused on the cancer biomarker discovery. She is developing
independent and significant predictive measures of tumor behavior for head and neck squamous cell
carcinoma and hepatocellular carcinoma. Quantitative measurements of protein expression either in
tissues or sera utilizing multidimensional separation and mass spectrometry have been major tools to
achieve the goal. A few peptide markers, which distinguished different tumor behaviors, have been
discovered successfully. The results will be integrated with the RNA expression data to identify the best
potential diagnostic and prognostic biomarkers to develop clinical diagnostics as a part of the system
biology approach.
ƒ
Dr. Lim’s skills in the mass spectrometry area have been directed toward neuropeptidomics.
Neuropeptides influence a wide range of physiological processes including feeding and body weight
regulation, circadian rhythms, sleep, pain, fear, anxiety, learning and memory, and reward
mechanisms. Quantitative and qualitative analyses of the vast array of neuropeptides are important for
understanding brain function. Many challenges, however, exist with their analysis, including low
concentrations and difficulties in labeling them for accurate quantitation. She has developed novel
labeling approaches to facilitate neuropeptide quantitation. This method has been utilized to find up- or
down-regulated neuropeptides under different stimuli to help in understanding the function of different
neuropeptide hormones.
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
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